Optimizing Production of Extracellular Laccase from Grammothele Subargentea CLPS No. 436 Strain

Autores
Saparrat, Mario Carlos Nazareno
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The production of extracellular laccase by the Grammothele subargentea CLPS no. 436 strain in liquid cultures grown on a carbon-limited basal medium was significantly enhanced when culture conditions, including the addition of CuSO4·5H2O or veratryl alcohol, were consecutively optimized. A laccase activity as high as 1954.5 mU ml−1 of liquid medium was obtained under optimum conditions, which corresponded to non-agitated cultures supplemented with 0.6 mM CuSO4·5H2O. Veratryl alcohol at 1 mM was less effective than CuSO4·5H2O for increasing laccase activity levels; the supplementation of veratryl alcohol resulted only in maximum levels of 44 mU ml−1 in non-agitated cultures.
Instituto de Botánica "Dr. Carlos Spegazzini"
Materia
Ciencias Naturales
Biología
Copper
culture agitation
extracellular ligninolytic enzymes
Grammothele subargentea
laccase
veratryl alcohol
white-rot fungi
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/140947
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/140947
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Optimizing Production of Extracellular Laccase from Grammothele Subargentea CLPS No. 436 StrainSaparrat, Mario Carlos NazarenoCiencias NaturalesBiologíaCopperculture agitationextracellular ligninolytic enzymesGrammothele subargentealaccaseveratryl alcoholwhite-rot fungiThe production of extracellular laccase by the Grammothele subargentea CLPS no. 436 strain in liquid cultures grown on a carbon-limited basal medium was significantly enhanced when culture conditions, including the addition of CuSO4·5H2O or veratryl alcohol, were consecutively optimized. A laccase activity as high as 1954.5 mU ml−1 of liquid medium was obtained under optimum conditions, which corresponded to non-agitated cultures supplemented with 0.6 mM CuSO4·5H2O. Veratryl alcohol at 1 mM was less effective than CuSO4·5H2O for increasing laccase activity levels; the supplementation of veratryl alcohol resulted only in maximum levels of 44 mU ml−1 in non-agitated cultures.Instituto de Botánica "Dr. Carlos Spegazzini"2004info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf583-586http://sedici.unlp.edu.ar/handle/10915/140947enginfo:eu-repo/semantics/altIdentifier/issn/0959-3993info:eu-repo/semantics/altIdentifier/issn/1573-0972info:eu-repo/semantics/altIdentifier/doi/10.1023/b:wibi.0000043167.39311.0ainfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:23:59Zoai:sedici.unlp.edu.ar:10915/140947Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:23:59.357SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Optimizing Production of Extracellular Laccase from Grammothele Subargentea CLPS No. 436 Strain
title Optimizing Production of Extracellular Laccase from Grammothele Subargentea CLPS No. 436 Strain
spellingShingle Optimizing Production of Extracellular Laccase from Grammothele Subargentea CLPS No. 436 Strain
Saparrat, Mario Carlos Nazareno
Ciencias Naturales
Biología
Copper
culture agitation
extracellular ligninolytic enzymes
Grammothele subargentea
laccase
veratryl alcohol
white-rot fungi
title_short Optimizing Production of Extracellular Laccase from Grammothele Subargentea CLPS No. 436 Strain
title_full Optimizing Production of Extracellular Laccase from Grammothele Subargentea CLPS No. 436 Strain
title_fullStr Optimizing Production of Extracellular Laccase from Grammothele Subargentea CLPS No. 436 Strain
title_full_unstemmed Optimizing Production of Extracellular Laccase from Grammothele Subargentea CLPS No. 436 Strain
title_sort Optimizing Production of Extracellular Laccase from Grammothele Subargentea CLPS No. 436 Strain
dc.creator.none.fl_str_mv Saparrat, Mario Carlos Nazareno
author Saparrat, Mario Carlos Nazareno
author_facet Saparrat, Mario Carlos Nazareno
author_role author
dc.subject.none.fl_str_mv Ciencias Naturales
Biología
Copper
culture agitation
extracellular ligninolytic enzymes
Grammothele subargentea
laccase
veratryl alcohol
white-rot fungi
topic Ciencias Naturales
Biología
Copper
culture agitation
extracellular ligninolytic enzymes
Grammothele subargentea
laccase
veratryl alcohol
white-rot fungi
dc.description.none.fl_txt_mv The production of extracellular laccase by the Grammothele subargentea CLPS no. 436 strain in liquid cultures grown on a carbon-limited basal medium was significantly enhanced when culture conditions, including the addition of CuSO4·5H2O or veratryl alcohol, were consecutively optimized. A laccase activity as high as 1954.5 mU ml−1 of liquid medium was obtained under optimum conditions, which corresponded to non-agitated cultures supplemented with 0.6 mM CuSO4·5H2O. Veratryl alcohol at 1 mM was less effective than CuSO4·5H2O for increasing laccase activity levels; the supplementation of veratryl alcohol resulted only in maximum levels of 44 mU ml−1 in non-agitated cultures.
Instituto de Botánica "Dr. Carlos Spegazzini"
description The production of extracellular laccase by the Grammothele subargentea CLPS no. 436 strain in liquid cultures grown on a carbon-limited basal medium was significantly enhanced when culture conditions, including the addition of CuSO4·5H2O or veratryl alcohol, were consecutively optimized. A laccase activity as high as 1954.5 mU ml−1 of liquid medium was obtained under optimum conditions, which corresponded to non-agitated cultures supplemented with 0.6 mM CuSO4·5H2O. Veratryl alcohol at 1 mM was less effective than CuSO4·5H2O for increasing laccase activity levels; the supplementation of veratryl alcohol resulted only in maximum levels of 44 mU ml−1 in non-agitated cultures.
publishDate 2004
dc.date.none.fl_str_mv 2004
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/140947
url http://sedici.unlp.edu.ar/handle/10915/140947
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0959-3993
info:eu-repo/semantics/altIdentifier/issn/1573-0972
info:eu-repo/semantics/altIdentifier/doi/10.1023/b:wibi.0000043167.39311.0a
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
583-586
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.22299

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