Paneth cells: histochemical and morphometric study in control and Solanum glaucophyllum intoxicated rabbits

Autores
Zanuzzi, Carolina Natalia; Fontana, Paula Andrea; Barbeito, Claudio Gustavo; Portiansky, Enrique Leo; Gimeno, Eduardo Juan
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The intestinal epithelium has a critical roll in host defence. One specialised cell type involved in this function is the Paneth cell, which secretes many substances with antimicrobial properties in response to different stimuli. Under pathological conditions, changes in the Paneth cell number, morphology and location as well as in granule number, morphology and composition have been reported. In the normal animal, 1,25-dihydroxyvitamin D3 participates in the maintenance of mineral homeostasis, immunomodulation and cell proliferation and differentiation. Solanum glaucophyllum, a calcinogenic plant containing high levels of 1,25-dihydroxyvitamin D3, is responsible for a condition known as enzootic calcinosis in ruminants, characterised by loss of body condition and mineralization of soft tissues. Using and established rabbit model, this study analyses the changes that rabbit Paneth cells undergo during intoxication with S. glaucophyllum. Male New Zealand white rabbits were experimentally intoxicated with S. glaucophyllum for 15 or 30 days. Lectin, immunohistochemical and morphometric studies were carried out on Paneth cells from samples of jejunum. SBA, DBA and WGA lectins bound to Paneth cells-granules in both normal and intoxicated rabbits, with more heterogenity in the labelling of granules from intoxicated rabbits. Paneth cells in both groups were immunonegative for lysosyme. A time and dose-dependent increase in the size and number of Paneth cells was found in both intoxicated groups. We suggest that the changes described in these cells may be directly or indirectly induced by S. glaucophyllum intoxication.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
Intestine
Plant-induced hypervitaminosis D3
Morphometry
Immunohistochemistry
Lectinhistochemistry
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/127973
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/127973
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network_name_str SEDICI (UNLP)
spelling Paneth cells: histochemical and morphometric study in control and Solanum glaucophyllum intoxicated rabbitsZanuzzi, Carolina NataliaFontana, Paula AndreaBarbeito, Claudio GustavoPortiansky, Enrique LeoGimeno, Eduardo JuanCiencias VeterinariasIntestinePlant-induced hypervitaminosis D3MorphometryImmunohistochemistryLectinhistochemistryThe intestinal epithelium has a critical roll in host defence. One specialised cell type involved in this function is the Paneth cell, which secretes many substances with antimicrobial properties in response to different stimuli. Under pathological conditions, changes in the Paneth cell number, morphology and location as well as in granule number, morphology and composition have been reported. In the normal animal, 1,25-dihydroxyvitamin D3 participates in the maintenance of mineral homeostasis, immunomodulation and cell proliferation and differentiation. Solanum glaucophyllum, a calcinogenic plant containing high levels of 1,25-dihydroxyvitamin D3, is responsible for a condition known as enzootic calcinosis in ruminants, characterised by loss of body condition and mineralization of soft tissues. Using and established rabbit model, this study analyses the changes that rabbit Paneth cells undergo during intoxication with S. glaucophyllum. Male New Zealand white rabbits were experimentally intoxicated with S. glaucophyllum for 15 or 30 days. Lectin, immunohistochemical and morphometric studies were carried out on Paneth cells from samples of jejunum. SBA, DBA and WGA lectins bound to Paneth cells-granules in both normal and intoxicated rabbits, with more heterogenity in the labelling of granules from intoxicated rabbits. Paneth cells in both groups were immunonegative for lysosyme. A time and dose-dependent increase in the size and number of Paneth cells was found in both intoxicated groups. We suggest that the changes described in these cells may be directly or indirectly induced by S. glaucophyllum intoxication.Facultad de Ciencias Veterinarias2008info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf93-100http://sedici.unlp.edu.ar/handle/10915/127973enginfo:eu-repo/semantics/altIdentifier/issn/1121-760Xinfo:eu-repo/semantics/altIdentifier/issn/2038-8306info:eu-repo/semantics/altIdentifier/pmid/18591155info:eu-repo/semantics/altIdentifier/doi/10.4081/1193info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0/Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:31:02Zoai:sedici.unlp.edu.ar:10915/127973Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:31:02.847SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Paneth cells: histochemical and morphometric study in control and Solanum glaucophyllum intoxicated rabbits
title Paneth cells: histochemical and morphometric study in control and Solanum glaucophyllum intoxicated rabbits
spellingShingle Paneth cells: histochemical and morphometric study in control and Solanum glaucophyllum intoxicated rabbits
Zanuzzi, Carolina Natalia
Ciencias Veterinarias
Intestine
Plant-induced hypervitaminosis D3
Morphometry
Immunohistochemistry
Lectinhistochemistry
title_short Paneth cells: histochemical and morphometric study in control and Solanum glaucophyllum intoxicated rabbits
title_full Paneth cells: histochemical and morphometric study in control and Solanum glaucophyllum intoxicated rabbits
title_fullStr Paneth cells: histochemical and morphometric study in control and Solanum glaucophyllum intoxicated rabbits
title_full_unstemmed Paneth cells: histochemical and morphometric study in control and Solanum glaucophyllum intoxicated rabbits
title_sort Paneth cells: histochemical and morphometric study in control and Solanum glaucophyllum intoxicated rabbits
dc.creator.none.fl_str_mv Zanuzzi, Carolina Natalia
Fontana, Paula Andrea
Barbeito, Claudio Gustavo
Portiansky, Enrique Leo
Gimeno, Eduardo Juan
author Zanuzzi, Carolina Natalia
author_facet Zanuzzi, Carolina Natalia
Fontana, Paula Andrea
Barbeito, Claudio Gustavo
Portiansky, Enrique Leo
Gimeno, Eduardo Juan
author_role author
author2 Fontana, Paula Andrea
Barbeito, Claudio Gustavo
Portiansky, Enrique Leo
Gimeno, Eduardo Juan
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Intestine
Plant-induced hypervitaminosis D3
Morphometry
Immunohistochemistry
Lectinhistochemistry
topic Ciencias Veterinarias
Intestine
Plant-induced hypervitaminosis D3
Morphometry
Immunohistochemistry
Lectinhistochemistry
dc.description.none.fl_txt_mv The intestinal epithelium has a critical roll in host defence. One specialised cell type involved in this function is the Paneth cell, which secretes many substances with antimicrobial properties in response to different stimuli. Under pathological conditions, changes in the Paneth cell number, morphology and location as well as in granule number, morphology and composition have been reported. In the normal animal, 1,25-dihydroxyvitamin D3 participates in the maintenance of mineral homeostasis, immunomodulation and cell proliferation and differentiation. Solanum glaucophyllum, a calcinogenic plant containing high levels of 1,25-dihydroxyvitamin D3, is responsible for a condition known as enzootic calcinosis in ruminants, characterised by loss of body condition and mineralization of soft tissues. Using and established rabbit model, this study analyses the changes that rabbit Paneth cells undergo during intoxication with S. glaucophyllum. Male New Zealand white rabbits were experimentally intoxicated with S. glaucophyllum for 15 or 30 days. Lectin, immunohistochemical and morphometric studies were carried out on Paneth cells from samples of jejunum. SBA, DBA and WGA lectins bound to Paneth cells-granules in both normal and intoxicated rabbits, with more heterogenity in the labelling of granules from intoxicated rabbits. Paneth cells in both groups were immunonegative for lysosyme. A time and dose-dependent increase in the size and number of Paneth cells was found in both intoxicated groups. We suggest that the changes described in these cells may be directly or indirectly induced by S. glaucophyllum intoxication.
Facultad de Ciencias Veterinarias
description The intestinal epithelium has a critical roll in host defence. One specialised cell type involved in this function is the Paneth cell, which secretes many substances with antimicrobial properties in response to different stimuli. Under pathological conditions, changes in the Paneth cell number, morphology and location as well as in granule number, morphology and composition have been reported. In the normal animal, 1,25-dihydroxyvitamin D3 participates in the maintenance of mineral homeostasis, immunomodulation and cell proliferation and differentiation. Solanum glaucophyllum, a calcinogenic plant containing high levels of 1,25-dihydroxyvitamin D3, is responsible for a condition known as enzootic calcinosis in ruminants, characterised by loss of body condition and mineralization of soft tissues. Using and established rabbit model, this study analyses the changes that rabbit Paneth cells undergo during intoxication with S. glaucophyllum. Male New Zealand white rabbits were experimentally intoxicated with S. glaucophyllum for 15 or 30 days. Lectin, immunohistochemical and morphometric studies were carried out on Paneth cells from samples of jejunum. SBA, DBA and WGA lectins bound to Paneth cells-granules in both normal and intoxicated rabbits, with more heterogenity in the labelling of granules from intoxicated rabbits. Paneth cells in both groups were immunonegative for lysosyme. A time and dose-dependent increase in the size and number of Paneth cells was found in both intoxicated groups. We suggest that the changes described in these cells may be directly or indirectly induced by S. glaucophyllum intoxication.
publishDate 2008
dc.date.none.fl_str_mv 2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/127973
url http://sedici.unlp.edu.ar/handle/10915/127973
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1121-760X
info:eu-repo/semantics/altIdentifier/issn/2038-8306
info:eu-repo/semantics/altIdentifier/pmid/18591155
info:eu-repo/semantics/altIdentifier/doi/10.4081/1193
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc/4.0/
Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
dc.format.none.fl_str_mv application/pdf
93-100
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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