Antiheart antibody-dependent cytotoxicity in the sera of mice chronically infected with <i>Trypanosoma cruzi</i>

Autores
Laguens, Rubén P.; Cabeza Meckert, Patricia Mildred; Chambó, Jorge Guillermo
Año de publicación
1988
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sera of mice chronically infected with Trypanosoma cruzi contain antibodies that bind to the surface of living adult syngeneic heart muscle cells. In a syngeneic system, with nonadherent spleen mononuclear cells as effector cells and cardiocytes as targets, antibody-dependent cytotoxicity (ADCC), revealed by the liberation of creatine phosphokinase from damaged cardiocytes, was observed after incorporation of serum samples from infected mice. Target damage was decreased after absorption with syngeneic myocardium, but absorption with T. cruzi epimastigotes or trypomastigotes or with syngeneic skeletal muscle had no effect on ADCC. No complement-dependent lysis against heart muscle cells was detected in the same serum samples. These observations indicate that serum from chronically chagasic mice contain antibodies that bind to the surface of living adult syngeneic cardiocytes and are able to exert ADCC, suggesting that they could play a role in the pathogenesis of the heart damage that occurs in Chagas' disease.
Facultad de Ciencias Médicas
Materia
Ciencias Médicas
Trypanosoma cruzi
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/108002
Acceder
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network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Antiheart antibody-dependent cytotoxicity in the sera of mice chronically infected with <i>Trypanosoma cruzi</i>Laguens, Rubén P.Cabeza Meckert, Patricia MildredChambó, Jorge GuillermoCiencias MédicasTrypanosoma cruziSera of mice chronically infected with Trypanosoma cruzi contain antibodies that bind to the surface of living adult syngeneic heart muscle cells. In a syngeneic system, with nonadherent spleen mononuclear cells as effector cells and cardiocytes as targets, antibody-dependent cytotoxicity (ADCC), revealed by the liberation of creatine phosphokinase from damaged cardiocytes, was observed after incorporation of serum samples from infected mice. Target damage was decreased after absorption with syngeneic myocardium, but absorption with T. cruzi epimastigotes or trypomastigotes or with syngeneic skeletal muscle had no effect on ADCC. No complement-dependent lysis against heart muscle cells was detected in the same serum samples. These observations indicate that serum from chronically chagasic mice contain antibodies that bind to the surface of living adult syngeneic cardiocytes and are able to exert ADCC, suggesting that they could play a role in the pathogenesis of the heart damage that occurs in Chagas' disease.Facultad de Ciencias Médicas1988info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf993-997http://sedici.unlp.edu.ar/handle/10915/108002enginfo:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC259404&blobtype=pdfinfo:eu-repo/semantics/altIdentifier/pmid/3126153info:eu-repo/semantics/altIdentifier/doi/10.1128/iai.56.4.993-997.1988info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:23:56Zoai:sedici.unlp.edu.ar:10915/108002Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:23:56.988SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Antiheart antibody-dependent cytotoxicity in the sera of mice chronically infected with <i>Trypanosoma cruzi</i>
title Antiheart antibody-dependent cytotoxicity in the sera of mice chronically infected with <i>Trypanosoma cruzi</i>
spellingShingle Antiheart antibody-dependent cytotoxicity in the sera of mice chronically infected with <i>Trypanosoma cruzi</i>
Laguens, Rubén P.
Ciencias Médicas
Trypanosoma cruzi
title_short Antiheart antibody-dependent cytotoxicity in the sera of mice chronically infected with <i>Trypanosoma cruzi</i>
title_full Antiheart antibody-dependent cytotoxicity in the sera of mice chronically infected with <i>Trypanosoma cruzi</i>
title_fullStr Antiheart antibody-dependent cytotoxicity in the sera of mice chronically infected with <i>Trypanosoma cruzi</i>
title_full_unstemmed Antiheart antibody-dependent cytotoxicity in the sera of mice chronically infected with <i>Trypanosoma cruzi</i>
title_sort Antiheart antibody-dependent cytotoxicity in the sera of mice chronically infected with <i>Trypanosoma cruzi</i>
dc.creator.none.fl_str_mv Laguens, Rubén P.
Cabeza Meckert, Patricia Mildred
Chambó, Jorge Guillermo
author Laguens, Rubén P.
author_facet Laguens, Rubén P.
Cabeza Meckert, Patricia Mildred
Chambó, Jorge Guillermo
author_role author
author2 Cabeza Meckert, Patricia Mildred
Chambó, Jorge Guillermo
author2_role author
author
dc.subject.none.fl_str_mv Ciencias Médicas
Trypanosoma cruzi
topic Ciencias Médicas
Trypanosoma cruzi
dc.description.none.fl_txt_mv Sera of mice chronically infected with Trypanosoma cruzi contain antibodies that bind to the surface of living adult syngeneic heart muscle cells. In a syngeneic system, with nonadherent spleen mononuclear cells as effector cells and cardiocytes as targets, antibody-dependent cytotoxicity (ADCC), revealed by the liberation of creatine phosphokinase from damaged cardiocytes, was observed after incorporation of serum samples from infected mice. Target damage was decreased after absorption with syngeneic myocardium, but absorption with T. cruzi epimastigotes or trypomastigotes or with syngeneic skeletal muscle had no effect on ADCC. No complement-dependent lysis against heart muscle cells was detected in the same serum samples. These observations indicate that serum from chronically chagasic mice contain antibodies that bind to the surface of living adult syngeneic cardiocytes and are able to exert ADCC, suggesting that they could play a role in the pathogenesis of the heart damage that occurs in Chagas' disease.
Facultad de Ciencias Médicas
description Sera of mice chronically infected with Trypanosoma cruzi contain antibodies that bind to the surface of living adult syngeneic heart muscle cells. In a syngeneic system, with nonadherent spleen mononuclear cells as effector cells and cardiocytes as targets, antibody-dependent cytotoxicity (ADCC), revealed by the liberation of creatine phosphokinase from damaged cardiocytes, was observed after incorporation of serum samples from infected mice. Target damage was decreased after absorption with syngeneic myocardium, but absorption with T. cruzi epimastigotes or trypomastigotes or with syngeneic skeletal muscle had no effect on ADCC. No complement-dependent lysis against heart muscle cells was detected in the same serum samples. These observations indicate that serum from chronically chagasic mice contain antibodies that bind to the surface of living adult syngeneic cardiocytes and are able to exert ADCC, suggesting that they could play a role in the pathogenesis of the heart damage that occurs in Chagas' disease.
publishDate 1988
dc.date.none.fl_str_mv 1988
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/108002
url http://sedici.unlp.edu.ar/handle/10915/108002
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC259404&blobtype=pdf
info:eu-repo/semantics/altIdentifier/pmid/3126153
info:eu-repo/semantics/altIdentifier/doi/10.1128/iai.56.4.993-997.1988
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
993-997
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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