Role of glucocorticoid receptor (GR) in white adipose tissue beiging

Autores
Martín, Florencia M.; Alzamendi, Ana; Harnichar, Alejandro Ezequiel; Castrogiovanni, Daniel Cayetano; Zubiría, María Guillermina; Spinedi, Eduardo Julio; Giovambattista, Andrés
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aim: Glucocorticoids (GCs) play a crucial role in energy homeostasis including white adipose tissue function; however, chronic GC excess is detrimental to mammals' health. White hypertrophic adiposity is a main factor for neuroendocrine-metabolic dysfunctions in monosodium L-glutamate (MSG)-damaged hypercorticosteronemic rat. Nevertheless, little is known about the receptor path in endogenous GC impact on white adipose tissueresident precursor cells to bring them into beige lineage. Thus, our aim was to explore whether transient/ chronic endogenous hypercorticosteronemia affects browning capacity in white adipose tissue pads from MSG rats during development. Main methods: Control and MSG male rats aged 30 and 90 days were 7-day exposed to cold conditions in order to stimulate wet white epidydimal adipose tissue (wEAT) beiging capacity. This procedure was also replicated in adrenalectomized rats. Key findings: Data indicated that whereas epidydimal white adipose tissue pads from prepubertal hypercorticosteronemic rats retained full expression of GR/MR genes resulting in a drastic reduction in wEAT beiging capacity, conversely, chronic hypercorticosteronemic adult MSG rats developed down-regulation of corticoid genes (and reduced GR cytosolic mediators) in wEAT pads and consequently partially restored local beiging capacity. Finally, wEAT pads from adrenalectomized rats revealed up-regulation of GR gene accompanied by full local beiging capacity. Significance: This study strongly supports a GR-dependent inhibitory effect of GC excess on white adipose tissue browning, an issue strongly supporting a key role of GR in the non-shivering thermogenic process. As a consequence, normalizing the GC milieu could be a relevant factor to handle dysmetabolism in white hyperadipose phenotypes.
Centro de Endocrinología Experimental y Aplicada
Instituto Multidisciplinario de Biología Celular
Materia
Biología
Glucocorticoid excess
Monosodim L-glutamate
Obesity
White adiposity browning
Non-shivering thermogenesis
Adrenalectomy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/159808
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/159808
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Role of glucocorticoid receptor (GR) in white adipose tissue beigingMartín, Florencia M.Alzamendi, AnaHarnichar, Alejandro EzequielCastrogiovanni, Daniel CayetanoZubiría, María GuillerminaSpinedi, Eduardo JulioGiovambattista, AndrésBiologíaGlucocorticoid excessMonosodim L-glutamateObesityWhite adiposity browningNon-shivering thermogenesisAdrenalectomyAim: Glucocorticoids (GCs) play a crucial role in energy homeostasis including white adipose tissue function; however, chronic GC excess is detrimental to mammals' health. White hypertrophic adiposity is a main factor for neuroendocrine-metabolic dysfunctions in monosodium L-glutamate (MSG)-damaged hypercorticosteronemic rat. Nevertheless, little is known about the receptor path in endogenous GC impact on white adipose tissueresident precursor cells to bring them into beige lineage. Thus, our aim was to explore whether transient/ chronic endogenous hypercorticosteronemia affects browning capacity in white adipose tissue pads from MSG rats during development. Main methods: Control and MSG male rats aged 30 and 90 days were 7-day exposed to cold conditions in order to stimulate wet white epidydimal adipose tissue (wEAT) beiging capacity. This procedure was also replicated in adrenalectomized rats. Key findings: Data indicated that whereas epidydimal white adipose tissue pads from prepubertal hypercorticosteronemic rats retained full expression of GR/MR genes resulting in a drastic reduction in wEAT beiging capacity, conversely, chronic hypercorticosteronemic adult MSG rats developed down-regulation of corticoid genes (and reduced GR cytosolic mediators) in wEAT pads and consequently partially restored local beiging capacity. Finally, wEAT pads from adrenalectomized rats revealed up-regulation of GR gene accompanied by full local beiging capacity. Significance: This study strongly supports a GR-dependent inhibitory effect of GC excess on white adipose tissue browning, an issue strongly supporting a key role of GR in the non-shivering thermogenic process. As a consequence, normalizing the GC milieu could be a relevant factor to handle dysmetabolism in white hyperadipose phenotypes.Centro de Endocrinología Experimental y AplicadaInstituto Multidisciplinario de Biología Celular2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/159808enginfo:eu-repo/semantics/altIdentifier/issn/1879-0631info:eu-repo/semantics/altIdentifier/issn/0024-3205info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lfs.2023.121681info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:41:47Zoai:sedici.unlp.edu.ar:10915/159808Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:41:47.61SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Role of glucocorticoid receptor (GR) in white adipose tissue beiging
title Role of glucocorticoid receptor (GR) in white adipose tissue beiging
spellingShingle Role of glucocorticoid receptor (GR) in white adipose tissue beiging
Martín, Florencia M.
Biología
Glucocorticoid excess
Monosodim L-glutamate
Obesity
White adiposity browning
Non-shivering thermogenesis
Adrenalectomy
title_short Role of glucocorticoid receptor (GR) in white adipose tissue beiging
title_full Role of glucocorticoid receptor (GR) in white adipose tissue beiging
title_fullStr Role of glucocorticoid receptor (GR) in white adipose tissue beiging
title_full_unstemmed Role of glucocorticoid receptor (GR) in white adipose tissue beiging
title_sort Role of glucocorticoid receptor (GR) in white adipose tissue beiging
dc.creator.none.fl_str_mv Martín, Florencia M.
Alzamendi, Ana
Harnichar, Alejandro Ezequiel
Castrogiovanni, Daniel Cayetano
Zubiría, María Guillermina
Spinedi, Eduardo Julio
Giovambattista, Andrés
author Martín, Florencia M.
author_facet Martín, Florencia M.
Alzamendi, Ana
Harnichar, Alejandro Ezequiel
Castrogiovanni, Daniel Cayetano
Zubiría, María Guillermina
Spinedi, Eduardo Julio
Giovambattista, Andrés
author_role author
author2 Alzamendi, Ana
Harnichar, Alejandro Ezequiel
Castrogiovanni, Daniel Cayetano
Zubiría, María Guillermina
Spinedi, Eduardo Julio
Giovambattista, Andrés
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Glucocorticoid excess
Monosodim L-glutamate
Obesity
White adiposity browning
Non-shivering thermogenesis
Adrenalectomy
topic Biología
Glucocorticoid excess
Monosodim L-glutamate
Obesity
White adiposity browning
Non-shivering thermogenesis
Adrenalectomy
dc.description.none.fl_txt_mv Aim: Glucocorticoids (GCs) play a crucial role in energy homeostasis including white adipose tissue function; however, chronic GC excess is detrimental to mammals' health. White hypertrophic adiposity is a main factor for neuroendocrine-metabolic dysfunctions in monosodium L-glutamate (MSG)-damaged hypercorticosteronemic rat. Nevertheless, little is known about the receptor path in endogenous GC impact on white adipose tissueresident precursor cells to bring them into beige lineage. Thus, our aim was to explore whether transient/ chronic endogenous hypercorticosteronemia affects browning capacity in white adipose tissue pads from MSG rats during development. Main methods: Control and MSG male rats aged 30 and 90 days were 7-day exposed to cold conditions in order to stimulate wet white epidydimal adipose tissue (wEAT) beiging capacity. This procedure was also replicated in adrenalectomized rats. Key findings: Data indicated that whereas epidydimal white adipose tissue pads from prepubertal hypercorticosteronemic rats retained full expression of GR/MR genes resulting in a drastic reduction in wEAT beiging capacity, conversely, chronic hypercorticosteronemic adult MSG rats developed down-regulation of corticoid genes (and reduced GR cytosolic mediators) in wEAT pads and consequently partially restored local beiging capacity. Finally, wEAT pads from adrenalectomized rats revealed up-regulation of GR gene accompanied by full local beiging capacity. Significance: This study strongly supports a GR-dependent inhibitory effect of GC excess on white adipose tissue browning, an issue strongly supporting a key role of GR in the non-shivering thermogenic process. As a consequence, normalizing the GC milieu could be a relevant factor to handle dysmetabolism in white hyperadipose phenotypes.
Centro de Endocrinología Experimental y Aplicada
Instituto Multidisciplinario de Biología Celular
description Aim: Glucocorticoids (GCs) play a crucial role in energy homeostasis including white adipose tissue function; however, chronic GC excess is detrimental to mammals' health. White hypertrophic adiposity is a main factor for neuroendocrine-metabolic dysfunctions in monosodium L-glutamate (MSG)-damaged hypercorticosteronemic rat. Nevertheless, little is known about the receptor path in endogenous GC impact on white adipose tissueresident precursor cells to bring them into beige lineage. Thus, our aim was to explore whether transient/ chronic endogenous hypercorticosteronemia affects browning capacity in white adipose tissue pads from MSG rats during development. Main methods: Control and MSG male rats aged 30 and 90 days were 7-day exposed to cold conditions in order to stimulate wet white epidydimal adipose tissue (wEAT) beiging capacity. This procedure was also replicated in adrenalectomized rats. Key findings: Data indicated that whereas epidydimal white adipose tissue pads from prepubertal hypercorticosteronemic rats retained full expression of GR/MR genes resulting in a drastic reduction in wEAT beiging capacity, conversely, chronic hypercorticosteronemic adult MSG rats developed down-regulation of corticoid genes (and reduced GR cytosolic mediators) in wEAT pads and consequently partially restored local beiging capacity. Finally, wEAT pads from adrenalectomized rats revealed up-regulation of GR gene accompanied by full local beiging capacity. Significance: This study strongly supports a GR-dependent inhibitory effect of GC excess on white adipose tissue browning, an issue strongly supporting a key role of GR in the non-shivering thermogenic process. As a consequence, normalizing the GC milieu could be a relevant factor to handle dysmetabolism in white hyperadipose phenotypes.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/159808
url http://sedici.unlp.edu.ar/handle/10915/159808
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1879-0631
info:eu-repo/semantics/altIdentifier/issn/0024-3205
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lfs.2023.121681
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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