Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen
- Autores
- Bottero, Daniela; Zurita, María Eugenia; Gaillard, María Emilia; Bartel, Erika Belén; Vercellini, María Clara; Hozbor, Daniela Flavia
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Bordetella bronchiseptica, a Gram-negative bacterium, causes chronic respiratory tract infections in a wide variety of mammalian hosts, including humans (albeit rarely). We recently designed Bordetella pertussis and Bordetella parapertussis experimental vaccines based on outer membrane vesicles (OMVs) derived from each pathogen, and we obtained protection against the respective infections in mice. Here, we demonstrated that OMVs derived from virulent-phase B. bronchiseptica (OMVBbvir⁺) protected mice against sublethal infections with different B. bronchiseptica strains, two isolated from farm animals and one isolated from a human patient. In all infections, we observed that the B. bronchiseptica loads were significantly reduced in the lungs of vaccinated animals; the lung-recovered CFU were decreased by ≥4 log units, compared with those detected in the lungs of nonimmunized animals (P < 0.001). In the OMVBbvir⁺-immunized mice, we detected IgG antibody titers against B. bronchiseptica whole-cell lysates, along with an immune serum having bacterial killing activity that both recognized B. bronchiseptica lipopolysaccharides and polypeptides such as GroEL and outer membrane protein C (OMPc) and demonstrated an essential protective capacity against B. bronchiseptica infection, as detected by passive in vivo transfer experiments. Stimulation of cultured splenocytes from immunized mice with OMVBbvir⁺ resulted in interleukin 5 (IL-5), gamma interferon (IFN-γ), and IL-17 production, indicating that the vesicles induced mixed Th2, Th1, and Th17 T-cell immune responses. We detected, by adoptive transfer assays, that spleen cells from OMVBbvir⁺-immunized mice also contributed to the observed protection against B. bronchiseptica infection. OMVs from avirulent-phase B. bronchiseptica and the resulting induced immune sera were also able to protect mice against B. bronchiseptica infection.
Facultad de Ciencias Exactas
Instituto de Biotecnologia y Biologia Molecular
Instituto de Estudios Inmunológicos y Fisiopatológicos - Materia
-
Ciencias Exactas
Biología
Bordetella bronchiseptica
outer membrane vesicles
phenotypic phases
vaccine - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/104593
Ver los metadatos del registro completo
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Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogenBottero, DanielaZurita, María EugeniaGaillard, María EmiliaBartel, Erika BelénVercellini, María ClaraHozbor, Daniela FlaviaCiencias ExactasBiologíaBordetella bronchisepticaouter membrane vesiclesphenotypic phasesvaccine<i>Bordetella bronchiseptica</i>, a Gram-negative bacterium, causes chronic respiratory tract infections in a wide variety of mammalian hosts, including humans (albeit rarely). We recently designed <i>Bordetella pertussis</i> and <i>Bordetella parapertussis</i> experimental vaccines based on outer membrane vesicles (OMVs) derived from each pathogen, and we obtained protection against the respective infections in mice. Here, we demonstrated that OMVs derived from virulent-phase <i>B. bronchiseptica</i> (OMVBbvir⁺) protected mice against sublethal infections with different <i>B. bronchiseptica</i> strains, two isolated from farm animals and one isolated from a human patient. In all infections, we observed that the <i>B. bronchiseptica</i> loads were significantly reduced in the lungs of vaccinated animals; the lung-recovered CFU were decreased by ≥4 log units, compared with those detected in the lungs of nonimmunized animals (P < 0.001). In the OMVBbvir⁺-immunized mice, we detected IgG antibody titers against <i>B. bronchiseptica</i> whole-cell lysates, along with an immune serum having bacterial killing activity that both recognized <i>B. bronchiseptica</i> lipopolysaccharides and polypeptides such as GroEL and outer membrane protein C (OMPc) and demonstrated an essential protective capacity against <i>B. bronchiseptica</i> infection, as detected by passive in vivo transfer experiments. Stimulation of cultured splenocytes from immunized mice with OMVBbvir⁺ resulted in interleukin 5 (IL-5), gamma interferon (IFN-γ), and IL-17 production, indicating that the vesicles induced mixed Th2, Th1, and Th17 T-cell immune responses. We detected, by adoptive transfer assays, that spleen cells from OMVBbvir⁺-immunized mice also contributed to the observed protection against <i>B. bronchiseptica</i> infection. OMVs from avirulent-phase <i>B. bronchiseptica</i> and the resulting induced immune sera were also able to protect mice against <i>B. bronchiseptica</i> infection.Facultad de Ciencias ExactasInstituto de Biotecnologia y Biologia MolecularInstituto de Estudios Inmunológicos y Fisiopatológicos2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/104593enginfo:eu-repo/semantics/altIdentifier/url/http://hdl.handle.net/11336/96975info:eu-repo/semantics/altIdentifier/url/https://aem.asm.org/content/84/4/e01877-17info:eu-repo/semantics/altIdentifier/issn/0099-2240info:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.01877-17info:eu-repo/semantics/altIdentifier/hdl/11336/96975info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:22:47Zoai:sedici.unlp.edu.ar:10915/104593Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:22:48.23SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen |
title |
Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen |
spellingShingle |
Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen Bottero, Daniela Ciencias Exactas Biología Bordetella bronchiseptica outer membrane vesicles phenotypic phases vaccine |
title_short |
Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen |
title_full |
Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen |
title_fullStr |
Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen |
title_full_unstemmed |
Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen |
title_sort |
Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen |
dc.creator.none.fl_str_mv |
Bottero, Daniela Zurita, María Eugenia Gaillard, María Emilia Bartel, Erika Belén Vercellini, María Clara Hozbor, Daniela Flavia |
author |
Bottero, Daniela |
author_facet |
Bottero, Daniela Zurita, María Eugenia Gaillard, María Emilia Bartel, Erika Belén Vercellini, María Clara Hozbor, Daniela Flavia |
author_role |
author |
author2 |
Zurita, María Eugenia Gaillard, María Emilia Bartel, Erika Belén Vercellini, María Clara Hozbor, Daniela Flavia |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Ciencias Exactas Biología Bordetella bronchiseptica outer membrane vesicles phenotypic phases vaccine |
topic |
Ciencias Exactas Biología Bordetella bronchiseptica outer membrane vesicles phenotypic phases vaccine |
dc.description.none.fl_txt_mv |
<i>Bordetella bronchiseptica</i>, a Gram-negative bacterium, causes chronic respiratory tract infections in a wide variety of mammalian hosts, including humans (albeit rarely). We recently designed <i>Bordetella pertussis</i> and <i>Bordetella parapertussis</i> experimental vaccines based on outer membrane vesicles (OMVs) derived from each pathogen, and we obtained protection against the respective infections in mice. Here, we demonstrated that OMVs derived from virulent-phase <i>B. bronchiseptica</i> (OMVBbvir⁺) protected mice against sublethal infections with different <i>B. bronchiseptica</i> strains, two isolated from farm animals and one isolated from a human patient. In all infections, we observed that the <i>B. bronchiseptica</i> loads were significantly reduced in the lungs of vaccinated animals; the lung-recovered CFU were decreased by ≥4 log units, compared with those detected in the lungs of nonimmunized animals (P < 0.001). In the OMVBbvir⁺-immunized mice, we detected IgG antibody titers against <i>B. bronchiseptica</i> whole-cell lysates, along with an immune serum having bacterial killing activity that both recognized <i>B. bronchiseptica</i> lipopolysaccharides and polypeptides such as GroEL and outer membrane protein C (OMPc) and demonstrated an essential protective capacity against <i>B. bronchiseptica</i> infection, as detected by passive in vivo transfer experiments. Stimulation of cultured splenocytes from immunized mice with OMVBbvir⁺ resulted in interleukin 5 (IL-5), gamma interferon (IFN-γ), and IL-17 production, indicating that the vesicles induced mixed Th2, Th1, and Th17 T-cell immune responses. We detected, by adoptive transfer assays, that spleen cells from OMVBbvir⁺-immunized mice also contributed to the observed protection against <i>B. bronchiseptica</i> infection. OMVs from avirulent-phase <i>B. bronchiseptica</i> and the resulting induced immune sera were also able to protect mice against <i>B. bronchiseptica</i> infection. Facultad de Ciencias Exactas Instituto de Biotecnologia y Biologia Molecular Instituto de Estudios Inmunológicos y Fisiopatológicos |
description |
<i>Bordetella bronchiseptica</i>, a Gram-negative bacterium, causes chronic respiratory tract infections in a wide variety of mammalian hosts, including humans (albeit rarely). We recently designed <i>Bordetella pertussis</i> and <i>Bordetella parapertussis</i> experimental vaccines based on outer membrane vesicles (OMVs) derived from each pathogen, and we obtained protection against the respective infections in mice. Here, we demonstrated that OMVs derived from virulent-phase <i>B. bronchiseptica</i> (OMVBbvir⁺) protected mice against sublethal infections with different <i>B. bronchiseptica</i> strains, two isolated from farm animals and one isolated from a human patient. In all infections, we observed that the <i>B. bronchiseptica</i> loads were significantly reduced in the lungs of vaccinated animals; the lung-recovered CFU were decreased by ≥4 log units, compared with those detected in the lungs of nonimmunized animals (P < 0.001). In the OMVBbvir⁺-immunized mice, we detected IgG antibody titers against <i>B. bronchiseptica</i> whole-cell lysates, along with an immune serum having bacterial killing activity that both recognized <i>B. bronchiseptica</i> lipopolysaccharides and polypeptides such as GroEL and outer membrane protein C (OMPc) and demonstrated an essential protective capacity against <i>B. bronchiseptica</i> infection, as detected by passive in vivo transfer experiments. Stimulation of cultured splenocytes from immunized mice with OMVBbvir⁺ resulted in interleukin 5 (IL-5), gamma interferon (IFN-γ), and IL-17 production, indicating that the vesicles induced mixed Th2, Th1, and Th17 T-cell immune responses. We detected, by adoptive transfer assays, that spleen cells from OMVBbvir⁺-immunized mice also contributed to the observed protection against <i>B. bronchiseptica</i> infection. OMVs from avirulent-phase <i>B. bronchiseptica</i> and the resulting induced immune sera were also able to protect mice against <i>B. bronchiseptica</i> infection. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
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http://sedici.unlp.edu.ar/handle/10915/104593 |
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http://sedici.unlp.edu.ar/handle/10915/104593 |
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eng |
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eng |
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openAccess |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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