Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen

Autores
Bottero, Daniela; Zurita, María Eugenia; Gaillard, María Emilia; Bartel, Erika Belén; Vercellini, María Clara; Hozbor, Daniela Flavia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bordetella bronchiseptica, a Gram-negative bacterium, causes chronic respiratory tract infections in a wide variety of mammalian hosts, including humans (albeit rarely). We recently designed Bordetella pertussis and Bordetella parapertussis experimental vaccines based on outer membrane vesicles (OMVs) derived from each pathogen, and we obtained protection against the respective infections in mice. Here, we demonstrated that OMVs derived from virulent-phase B. bronchiseptica (OMVBbvir⁺) protected mice against sublethal infections with different B. bronchiseptica strains, two isolated from farm animals and one isolated from a human patient. In all infections, we observed that the B. bronchiseptica loads were significantly reduced in the lungs of vaccinated animals; the lung-recovered CFU were decreased by ≥4 log units, compared with those detected in the lungs of nonimmunized animals (P < 0.001). In the OMVBbvir⁺-immunized mice, we detected IgG antibody titers against B. bronchiseptica whole-cell lysates, along with an immune serum having bacterial killing activity that both recognized B. bronchiseptica lipopolysaccharides and polypeptides such as GroEL and outer membrane protein C (OMPc) and demonstrated an essential protective capacity against B. bronchiseptica infection, as detected by passive in vivo transfer experiments. Stimulation of cultured splenocytes from immunized mice with OMVBbvir⁺ resulted in interleukin 5 (IL-5), gamma interferon (IFN-γ), and IL-17 production, indicating that the vesicles induced mixed Th2, Th1, and Th17 T-cell immune responses. We detected, by adoptive transfer assays, that spleen cells from OMVBbvir⁺-immunized mice also contributed to the observed protection against B. bronchiseptica infection. OMVs from avirulent-phase B. bronchiseptica and the resulting induced immune sera were also able to protect mice against B. bronchiseptica infection.
Facultad de Ciencias Exactas
Instituto de Biotecnologia y Biologia Molecular
Instituto de Estudios Inmunológicos y Fisiopatológicos
Materia
Ciencias Exactas
Biología
Bordetella bronchiseptica
outer membrane vesicles
phenotypic phases
vaccine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/104593

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network_name_str SEDICI (UNLP)
spelling Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogenBottero, DanielaZurita, María EugeniaGaillard, María EmiliaBartel, Erika BelénVercellini, María ClaraHozbor, Daniela FlaviaCiencias ExactasBiologíaBordetella bronchisepticaouter membrane vesiclesphenotypic phasesvaccine<i>Bordetella bronchiseptica</i>, a Gram-negative bacterium, causes chronic respiratory tract infections in a wide variety of mammalian hosts, including humans (albeit rarely). We recently designed <i>Bordetella pertussis</i> and <i>Bordetella parapertussis</i> experimental vaccines based on outer membrane vesicles (OMVs) derived from each pathogen, and we obtained protection against the respective infections in mice. Here, we demonstrated that OMVs derived from virulent-phase <i>B. bronchiseptica</i> (OMVBbvir⁺) protected mice against sublethal infections with different <i>B. bronchiseptica</i> strains, two isolated from farm animals and one isolated from a human patient. In all infections, we observed that the <i>B. bronchiseptica</i> loads were significantly reduced in the lungs of vaccinated animals; the lung-recovered CFU were decreased by ≥4 log units, compared with those detected in the lungs of nonimmunized animals (P < 0.001). In the OMVBbvir⁺-immunized mice, we detected IgG antibody titers against <i>B. bronchiseptica</i> whole-cell lysates, along with an immune serum having bacterial killing activity that both recognized <i>B. bronchiseptica</i> lipopolysaccharides and polypeptides such as GroEL and outer membrane protein C (OMPc) and demonstrated an essential protective capacity against <i>B. bronchiseptica</i> infection, as detected by passive in vivo transfer experiments. Stimulation of cultured splenocytes from immunized mice with OMVBbvir⁺ resulted in interleukin 5 (IL-5), gamma interferon (IFN-γ), and IL-17 production, indicating that the vesicles induced mixed Th2, Th1, and Th17 T-cell immune responses. We detected, by adoptive transfer assays, that spleen cells from OMVBbvir⁺-immunized mice also contributed to the observed protection against <i>B. bronchiseptica</i> infection. OMVs from avirulent-phase <i>B. bronchiseptica</i> and the resulting induced immune sera were also able to protect mice against <i>B. bronchiseptica</i> infection.Facultad de Ciencias ExactasInstituto de Biotecnologia y Biologia MolecularInstituto de Estudios Inmunológicos y Fisiopatológicos2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/104593enginfo:eu-repo/semantics/altIdentifier/url/http://hdl.handle.net/11336/96975info:eu-repo/semantics/altIdentifier/url/https://aem.asm.org/content/84/4/e01877-17info:eu-repo/semantics/altIdentifier/issn/0099-2240info:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.01877-17info:eu-repo/semantics/altIdentifier/hdl/11336/96975info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:22:47Zoai:sedici.unlp.edu.ar:10915/104593Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:22:48.23SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen
title Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen
spellingShingle Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen
Bottero, Daniela
Ciencias Exactas
Biología
Bordetella bronchiseptica
outer membrane vesicles
phenotypic phases
vaccine
title_short Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen
title_full Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen
title_fullStr Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen
title_full_unstemmed Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen
title_sort Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen
dc.creator.none.fl_str_mv Bottero, Daniela
Zurita, María Eugenia
Gaillard, María Emilia
Bartel, Erika Belén
Vercellini, María Clara
Hozbor, Daniela Flavia
author Bottero, Daniela
author_facet Bottero, Daniela
Zurita, María Eugenia
Gaillard, María Emilia
Bartel, Erika Belén
Vercellini, María Clara
Hozbor, Daniela Flavia
author_role author
author2 Zurita, María Eugenia
Gaillard, María Emilia
Bartel, Erika Belén
Vercellini, María Clara
Hozbor, Daniela Flavia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Biología
Bordetella bronchiseptica
outer membrane vesicles
phenotypic phases
vaccine
topic Ciencias Exactas
Biología
Bordetella bronchiseptica
outer membrane vesicles
phenotypic phases
vaccine
dc.description.none.fl_txt_mv <i>Bordetella bronchiseptica</i>, a Gram-negative bacterium, causes chronic respiratory tract infections in a wide variety of mammalian hosts, including humans (albeit rarely). We recently designed <i>Bordetella pertussis</i> and <i>Bordetella parapertussis</i> experimental vaccines based on outer membrane vesicles (OMVs) derived from each pathogen, and we obtained protection against the respective infections in mice. Here, we demonstrated that OMVs derived from virulent-phase <i>B. bronchiseptica</i> (OMVBbvir⁺) protected mice against sublethal infections with different <i>B. bronchiseptica</i> strains, two isolated from farm animals and one isolated from a human patient. In all infections, we observed that the <i>B. bronchiseptica</i> loads were significantly reduced in the lungs of vaccinated animals; the lung-recovered CFU were decreased by ≥4 log units, compared with those detected in the lungs of nonimmunized animals (P < 0.001). In the OMVBbvir⁺-immunized mice, we detected IgG antibody titers against <i>B. bronchiseptica</i> whole-cell lysates, along with an immune serum having bacterial killing activity that both recognized <i>B. bronchiseptica</i> lipopolysaccharides and polypeptides such as GroEL and outer membrane protein C (OMPc) and demonstrated an essential protective capacity against <i>B. bronchiseptica</i> infection, as detected by passive in vivo transfer experiments. Stimulation of cultured splenocytes from immunized mice with OMVBbvir⁺ resulted in interleukin 5 (IL-5), gamma interferon (IFN-γ), and IL-17 production, indicating that the vesicles induced mixed Th2, Th1, and Th17 T-cell immune responses. We detected, by adoptive transfer assays, that spleen cells from OMVBbvir⁺-immunized mice also contributed to the observed protection against <i>B. bronchiseptica</i> infection. OMVs from avirulent-phase <i>B. bronchiseptica</i> and the resulting induced immune sera were also able to protect mice against <i>B. bronchiseptica</i> infection.
Facultad de Ciencias Exactas
Instituto de Biotecnologia y Biologia Molecular
Instituto de Estudios Inmunológicos y Fisiopatológicos
description <i>Bordetella bronchiseptica</i>, a Gram-negative bacterium, causes chronic respiratory tract infections in a wide variety of mammalian hosts, including humans (albeit rarely). We recently designed <i>Bordetella pertussis</i> and <i>Bordetella parapertussis</i> experimental vaccines based on outer membrane vesicles (OMVs) derived from each pathogen, and we obtained protection against the respective infections in mice. Here, we demonstrated that OMVs derived from virulent-phase <i>B. bronchiseptica</i> (OMVBbvir⁺) protected mice against sublethal infections with different <i>B. bronchiseptica</i> strains, two isolated from farm animals and one isolated from a human patient. In all infections, we observed that the <i>B. bronchiseptica</i> loads were significantly reduced in the lungs of vaccinated animals; the lung-recovered CFU were decreased by ≥4 log units, compared with those detected in the lungs of nonimmunized animals (P < 0.001). In the OMVBbvir⁺-immunized mice, we detected IgG antibody titers against <i>B. bronchiseptica</i> whole-cell lysates, along with an immune serum having bacterial killing activity that both recognized <i>B. bronchiseptica</i> lipopolysaccharides and polypeptides such as GroEL and outer membrane protein C (OMPc) and demonstrated an essential protective capacity against <i>B. bronchiseptica</i> infection, as detected by passive in vivo transfer experiments. Stimulation of cultured splenocytes from immunized mice with OMVBbvir⁺ resulted in interleukin 5 (IL-5), gamma interferon (IFN-γ), and IL-17 production, indicating that the vesicles induced mixed Th2, Th1, and Th17 T-cell immune responses. We detected, by adoptive transfer assays, that spleen cells from OMVBbvir⁺-immunized mice also contributed to the observed protection against <i>B. bronchiseptica</i> infection. OMVs from avirulent-phase <i>B. bronchiseptica</i> and the resulting induced immune sera were also able to protect mice against <i>B. bronchiseptica</i> infection.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
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format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/104593
url http://sedici.unlp.edu.ar/handle/10915/104593
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language eng
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info:eu-repo/semantics/altIdentifier/url/https://aem.asm.org/content/84/4/e01877-17
info:eu-repo/semantics/altIdentifier/issn/0099-2240
info:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.01877-17
info:eu-repo/semantics/altIdentifier/hdl/11336/96975
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
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