A highly stable, nondigestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs

Autores
Brola, Tabata Romina; Dreon, Marcos Sebastián; Qiu, Jian-Wen; Heras, Horacio
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The acquisition of egg protection is vital for species survival. Poisonous eggs from Pomacea apple snails have defensive macromolecules for protection. Here we isolated and characterized a novel lectin called PdPV1 that is massively accumulated in the eggs of Pomacea diffusa and seems part of its protective cocktail. The native protein, an oligomer of ca. 256 kDa, has high structural stability, withstanding 15 min boiling and denaturing by sodium dodecyl sulphate. It resists in vitro proteinase digestion and displays structural stability between pH 2.0-12.0 and up to 85 °C. These properties, as well as its subunit sequences, glycosylation pattern, presence of carotenoids, size, and global shape resemble those of its orthologs from other Pomacea. Further, like members of the canaliculata clade, PdPV1 is recovered unchanged in faeces of mice ingesting it, supporting an antinutritive defensive function. PdPV1 also displays a strong hemagglutinating activity specifically recognizing selected ganglioside motifs with high affinity. This activity is only shared with PsSC, a perivitelline from the same clade (bridgesii clade). As a whole, these results indicate that species in the genus Pomacea have diversified their eggs defences: Those from the bridgesii clade are protected mostly by non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades, indicating apple snail egg defensive strategies are clade-specific. The harsh gastrointestinal environment of predators would have favoured their appearance, extending by convergent evolution the presence of plant-like highly stable lectins, a strategy not reported in other animals.
Instituto de Investigaciones Bioquímicas de La Plata
Facultad de Ciencias Naturales y Museo
Materia
Biología
Ampullariidae
Egg defense
Gastropods
Glycan array
Lectin activity
Protein structure
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/126164

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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling A highly stable, nondigestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggsBrola, Tabata RominaDreon, Marcos SebastiánQiu, Jian-WenHeras, HoracioBiologíaAmpullariidaeEgg defenseGastropodsGlycan arrayLectin activityProtein structureThe acquisition of egg protection is vital for species survival. Poisonous eggs from Pomacea apple snails have defensive macromolecules for protection. Here we isolated and characterized a novel lectin called PdPV1 that is massively accumulated in the eggs of Pomacea diffusa and seems part of its protective cocktail. The native protein, an oligomer of ca. 256 kDa, has high structural stability, withstanding 15 min boiling and denaturing by sodium dodecyl sulphate. It resists in vitro proteinase digestion and displays structural stability between pH 2.0-12.0 and up to 85 °C. These properties, as well as its subunit sequences, glycosylation pattern, presence of carotenoids, size, and global shape resemble those of its orthologs from other Pomacea. Further, like members of the canaliculata clade, PdPV1 is recovered unchanged in faeces of mice ingesting it, supporting an antinutritive defensive function. PdPV1 also displays a strong hemagglutinating activity specifically recognizing selected ganglioside motifs with high affinity. This activity is only shared with PsSC, a perivitelline from the same clade (bridgesii clade). As a whole, these results indicate that species in the genus Pomacea have diversified their eggs defences: Those from the bridgesii clade are protected mostly by non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades, indicating apple snail egg defensive strategies are clade-specific. The harsh gastrointestinal environment of predators would have favoured their appearance, extending by convergent evolution the presence of plant-like highly stable lectins, a strategy not reported in other animals.Instituto de Investigaciones Bioquímicas de La PlataFacultad de Ciencias Naturales y Museo2020-10-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/126164enginfo:eu-repo/semantics/altIdentifier/issn/1477-9145info:eu-repo/semantics/altIdentifier/issn/0022-0949info:eu-repo/semantics/altIdentifier/pmid/32719049info:eu-repo/semantics/altIdentifier/doi/10.1242/jeb.231878info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:30:17Zoai:sedici.unlp.edu.ar:10915/126164Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:30:18.155SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv A highly stable, nondigestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs
title A highly stable, nondigestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs
spellingShingle A highly stable, nondigestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs
Brola, Tabata Romina
Biología
Ampullariidae
Egg defense
Gastropods
Glycan array
Lectin activity
Protein structure
title_short A highly stable, nondigestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs
title_full A highly stable, nondigestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs
title_fullStr A highly stable, nondigestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs
title_full_unstemmed A highly stable, nondigestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs
title_sort A highly stable, nondigestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs
dc.creator.none.fl_str_mv Brola, Tabata Romina
Dreon, Marcos Sebastián
Qiu, Jian-Wen
Heras, Horacio
author Brola, Tabata Romina
author_facet Brola, Tabata Romina
Dreon, Marcos Sebastián
Qiu, Jian-Wen
Heras, Horacio
author_role author
author2 Dreon, Marcos Sebastián
Qiu, Jian-Wen
Heras, Horacio
author2_role author
author
author
dc.subject.none.fl_str_mv Biología
Ampullariidae
Egg defense
Gastropods
Glycan array
Lectin activity
Protein structure
topic Biología
Ampullariidae
Egg defense
Gastropods
Glycan array
Lectin activity
Protein structure
dc.description.none.fl_txt_mv The acquisition of egg protection is vital for species survival. Poisonous eggs from Pomacea apple snails have defensive macromolecules for protection. Here we isolated and characterized a novel lectin called PdPV1 that is massively accumulated in the eggs of Pomacea diffusa and seems part of its protective cocktail. The native protein, an oligomer of ca. 256 kDa, has high structural stability, withstanding 15 min boiling and denaturing by sodium dodecyl sulphate. It resists in vitro proteinase digestion and displays structural stability between pH 2.0-12.0 and up to 85 °C. These properties, as well as its subunit sequences, glycosylation pattern, presence of carotenoids, size, and global shape resemble those of its orthologs from other Pomacea. Further, like members of the canaliculata clade, PdPV1 is recovered unchanged in faeces of mice ingesting it, supporting an antinutritive defensive function. PdPV1 also displays a strong hemagglutinating activity specifically recognizing selected ganglioside motifs with high affinity. This activity is only shared with PsSC, a perivitelline from the same clade (bridgesii clade). As a whole, these results indicate that species in the genus Pomacea have diversified their eggs defences: Those from the bridgesii clade are protected mostly by non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades, indicating apple snail egg defensive strategies are clade-specific. The harsh gastrointestinal environment of predators would have favoured their appearance, extending by convergent evolution the presence of plant-like highly stable lectins, a strategy not reported in other animals.
Instituto de Investigaciones Bioquímicas de La Plata
Facultad de Ciencias Naturales y Museo
description The acquisition of egg protection is vital for species survival. Poisonous eggs from Pomacea apple snails have defensive macromolecules for protection. Here we isolated and characterized a novel lectin called PdPV1 that is massively accumulated in the eggs of Pomacea diffusa and seems part of its protective cocktail. The native protein, an oligomer of ca. 256 kDa, has high structural stability, withstanding 15 min boiling and denaturing by sodium dodecyl sulphate. It resists in vitro proteinase digestion and displays structural stability between pH 2.0-12.0 and up to 85 °C. These properties, as well as its subunit sequences, glycosylation pattern, presence of carotenoids, size, and global shape resemble those of its orthologs from other Pomacea. Further, like members of the canaliculata clade, PdPV1 is recovered unchanged in faeces of mice ingesting it, supporting an antinutritive defensive function. PdPV1 also displays a strong hemagglutinating activity specifically recognizing selected ganglioside motifs with high affinity. This activity is only shared with PsSC, a perivitelline from the same clade (bridgesii clade). As a whole, these results indicate that species in the genus Pomacea have diversified their eggs defences: Those from the bridgesii clade are protected mostly by non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades, indicating apple snail egg defensive strategies are clade-specific. The harsh gastrointestinal environment of predators would have favoured their appearance, extending by convergent evolution the presence of plant-like highly stable lectins, a strategy not reported in other animals.
publishDate 2020
dc.date.none.fl_str_mv 2020-10-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/126164
url http://sedici.unlp.edu.ar/handle/10915/126164
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1477-9145
info:eu-repo/semantics/altIdentifier/issn/0022-0949
info:eu-repo/semantics/altIdentifier/pmid/32719049
info:eu-repo/semantics/altIdentifier/doi/10.1242/jeb.231878
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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