Proinflammatory response of human trophoblastic cells to Brucella abortus infection and upon interactions with infected phagocytes

Autores
Fernández, Andrea G.; Ferrero, Mariana C.; Hielpos, M. Soledad; Fossati, Carlos Alberto; Baldi, Pablo C.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trophoblasts are targets of infection by Brucella spp. but their role in the pathophysiology of pregnancy complications of brucellosis is unknown. Here we show that Brucella abortus invades and replicates in the human trophoblastic cell line Swan-71 and that the intracellular survival of the bacterium depends on a functional virB operon. The infection elicited significant increments of interleukin 8 (IL8), monocyte chemotactic protein 1 (MCP-1), and IL6 secretion, but levels of IL1beta and tumor necrosis factor-alpha (TNF-alpha) did not vary significantly. Such proinflammatory response was not modified by the absence of the Brucella TIR domain-containing proteins BtpA and BtpB. The stimulation of Swan-71 cells with conditioned medium (CM) from B. abortus-infected human monocytes (THP-1 cells) or macrophages induced a significant increase of IL8, MCP-1 and IL6 as compared to stimulation with CM from non-infected cells. Similar results were obtained when stimulation was performed with CM from infected neutrophils. Neutralization studies showed that IL1beta and/or TNF-alpha mediated the stimulating effects of CM from infected phagocytes. Reciprocally, stimulation of monocytes and neutrophils with CM from Brucella-infected trophoblasts increased IL8 and/or IL6 secretion. These results suggest that human trophoblasts may provide a local inflammatory environment during B. abortus infections either through a direct response to the pathogen or through interactions with monocytes/macrophages or neutrophils, potentially contributing to the pregnancy complications of brucellosis.
Instituto de Estudios Inmunológicos y Fisiopatológicos
Materia
Biología
Ciencias Exactas
Brucella
Cellular interactions
Inflammatory response
Trophoblast
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/146065

id SEDICI_ac39cfbf9c0657297fbe200b4db4f14f
oai_identifier_str oai:sedici.unlp.edu.ar:10915/146065
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Proinflammatory response of human trophoblastic cells to Brucella abortus infection and upon interactions with infected phagocytesFernández, Andrea G.Ferrero, Mariana C.Hielpos, M. SoledadFossati, Carlos AlbertoBaldi, Pablo C.BiologíaCiencias ExactasBrucellaCellular interactionsInflammatory responseTrophoblastTrophoblasts are targets of infection by Brucella spp. but their role in the pathophysiology of pregnancy complications of brucellosis is unknown. Here we show that Brucella abortus invades and replicates in the human trophoblastic cell line Swan-71 and that the intracellular survival of the bacterium depends on a functional virB operon. The infection elicited significant increments of interleukin 8 (IL8), monocyte chemotactic protein 1 (MCP-1), and IL6 secretion, but levels of IL1beta and tumor necrosis factor-alpha (TNF-alpha) did not vary significantly. Such proinflammatory response was not modified by the absence of the Brucella TIR domain-containing proteins BtpA and BtpB. The stimulation of Swan-71 cells with conditioned medium (CM) from B. abortus-infected human monocytes (THP-1 cells) or macrophages induced a significant increase of IL8, MCP-1 and IL6 as compared to stimulation with CM from non-infected cells. Similar results were obtained when stimulation was performed with CM from infected neutrophils. Neutralization studies showed that IL1beta and/or TNF-alpha mediated the stimulating effects of CM from infected phagocytes. Reciprocally, stimulation of monocytes and neutrophils with CM from Brucella-infected trophoblasts increased IL8 and/or IL6 secretion. These results suggest that human trophoblasts may provide a local inflammatory environment during B. abortus infections either through a direct response to the pathogen or through interactions with monocytes/macrophages or neutrophils, potentially contributing to the pregnancy complications of brucellosis.Instituto de Estudios Inmunológicos y Fisiopatológicos2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/146065enginfo:eu-repo/semantics/altIdentifier/issn/1529-7268info:eu-repo/semantics/altIdentifier/issn/0006-3363info:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.115.131706info:eu-repo/semantics/altIdentifier/pmid/26792938info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:26Zoai:sedici.unlp.edu.ar:10915/146065Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:27.23SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Proinflammatory response of human trophoblastic cells to Brucella abortus infection and upon interactions with infected phagocytes
title Proinflammatory response of human trophoblastic cells to Brucella abortus infection and upon interactions with infected phagocytes
spellingShingle Proinflammatory response of human trophoblastic cells to Brucella abortus infection and upon interactions with infected phagocytes
Fernández, Andrea G.
Biología
Ciencias Exactas
Brucella
Cellular interactions
Inflammatory response
Trophoblast
title_short Proinflammatory response of human trophoblastic cells to Brucella abortus infection and upon interactions with infected phagocytes
title_full Proinflammatory response of human trophoblastic cells to Brucella abortus infection and upon interactions with infected phagocytes
title_fullStr Proinflammatory response of human trophoblastic cells to Brucella abortus infection and upon interactions with infected phagocytes
title_full_unstemmed Proinflammatory response of human trophoblastic cells to Brucella abortus infection and upon interactions with infected phagocytes
title_sort Proinflammatory response of human trophoblastic cells to Brucella abortus infection and upon interactions with infected phagocytes
dc.creator.none.fl_str_mv Fernández, Andrea G.
Ferrero, Mariana C.
Hielpos, M. Soledad
Fossati, Carlos Alberto
Baldi, Pablo C.
author Fernández, Andrea G.
author_facet Fernández, Andrea G.
Ferrero, Mariana C.
Hielpos, M. Soledad
Fossati, Carlos Alberto
Baldi, Pablo C.
author_role author
author2 Ferrero, Mariana C.
Hielpos, M. Soledad
Fossati, Carlos Alberto
Baldi, Pablo C.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Biología
Ciencias Exactas
Brucella
Cellular interactions
Inflammatory response
Trophoblast
topic Biología
Ciencias Exactas
Brucella
Cellular interactions
Inflammatory response
Trophoblast
dc.description.none.fl_txt_mv Trophoblasts are targets of infection by Brucella spp. but their role in the pathophysiology of pregnancy complications of brucellosis is unknown. Here we show that Brucella abortus invades and replicates in the human trophoblastic cell line Swan-71 and that the intracellular survival of the bacterium depends on a functional virB operon. The infection elicited significant increments of interleukin 8 (IL8), monocyte chemotactic protein 1 (MCP-1), and IL6 secretion, but levels of IL1beta and tumor necrosis factor-alpha (TNF-alpha) did not vary significantly. Such proinflammatory response was not modified by the absence of the Brucella TIR domain-containing proteins BtpA and BtpB. The stimulation of Swan-71 cells with conditioned medium (CM) from B. abortus-infected human monocytes (THP-1 cells) or macrophages induced a significant increase of IL8, MCP-1 and IL6 as compared to stimulation with CM from non-infected cells. Similar results were obtained when stimulation was performed with CM from infected neutrophils. Neutralization studies showed that IL1beta and/or TNF-alpha mediated the stimulating effects of CM from infected phagocytes. Reciprocally, stimulation of monocytes and neutrophils with CM from Brucella-infected trophoblasts increased IL8 and/or IL6 secretion. These results suggest that human trophoblasts may provide a local inflammatory environment during B. abortus infections either through a direct response to the pathogen or through interactions with monocytes/macrophages or neutrophils, potentially contributing to the pregnancy complications of brucellosis.
Instituto de Estudios Inmunológicos y Fisiopatológicos
description Trophoblasts are targets of infection by Brucella spp. but their role in the pathophysiology of pregnancy complications of brucellosis is unknown. Here we show that Brucella abortus invades and replicates in the human trophoblastic cell line Swan-71 and that the intracellular survival of the bacterium depends on a functional virB operon. The infection elicited significant increments of interleukin 8 (IL8), monocyte chemotactic protein 1 (MCP-1), and IL6 secretion, but levels of IL1beta and tumor necrosis factor-alpha (TNF-alpha) did not vary significantly. Such proinflammatory response was not modified by the absence of the Brucella TIR domain-containing proteins BtpA and BtpB. The stimulation of Swan-71 cells with conditioned medium (CM) from B. abortus-infected human monocytes (THP-1 cells) or macrophages induced a significant increase of IL8, MCP-1 and IL6 as compared to stimulation with CM from non-infected cells. Similar results were obtained when stimulation was performed with CM from infected neutrophils. Neutralization studies showed that IL1beta and/or TNF-alpha mediated the stimulating effects of CM from infected phagocytes. Reciprocally, stimulation of monocytes and neutrophils with CM from Brucella-infected trophoblasts increased IL8 and/or IL6 secretion. These results suggest that human trophoblasts may provide a local inflammatory environment during B. abortus infections either through a direct response to the pathogen or through interactions with monocytes/macrophages or neutrophils, potentially contributing to the pregnancy complications of brucellosis.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/146065
url http://sedici.unlp.edu.ar/handle/10915/146065
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1529-7268
info:eu-repo/semantics/altIdentifier/issn/0006-3363
info:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.115.131706
info:eu-repo/semantics/altIdentifier/pmid/26792938
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1844616203975262208
score 13.070432