DNA fingerprint and pathotype diversity of Pyricularia oryzae populations from Argentina

Autores
Consolo, Verónica Fabiana; Cordo, Cristina Alicia; Salerno, Graciela Lidia
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The genetic diversity of the rice blast pathogen, Pyricularia oryzae, was analysed in rice-growing provinces of Argentina. A total of 161 isolates of the fungus was collected from 15 rice cultivars at nine locations during 2000-05 and characterised using Pot2-DNA fingerprinting. Based on DNA analysis (isolates with ≥70% band similarity), five lineages were identified and designated A, B, C, D and E, with 11, 22, 4, 1 and 4 haplotypes identified, respectively. The predominant lineage, B, representing 38% of the collected isolates, was recovered from four cultivars in five locations. In contrast to lineages A and B, which did not contain a dominant haplotype, a single haplotype predominated in lineages C and E. Isolates representing all haplotypes were examined for virulence on a set of differential rice cultivars, near-isogenic lines and commercial cultivars commonly grown in Argentina, revealing 41 pathotypes and 24 international races. There was no significant association between DNA fingerprint similarities and pathotypes. Overall, these data indicated that populations of P. oryzae in Argentina are genetically simple and predominantly clonal yet have a high pathotype diversity.
Centro de Investigaciones en Fitopatología
Materia
Ciencias Agrarias
Magnaporthe oryzae
population structure
rice breeding
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/144408
Acceder
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network_acronym_str SEDICI
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network_name_str SEDICI (UNLP)
spelling DNA fingerprint and pathotype diversity of Pyricularia oryzae populations from ArgentinaConsolo, Verónica FabianaCordo, Cristina AliciaSalerno, Graciela LidiaCiencias AgrariasMagnaporthe oryzaepopulation structurerice breedingThe genetic diversity of the rice blast pathogen, <i>Pyricularia oryzae</i>, was analysed in rice-growing provinces of Argentina. A total of 161 isolates of the fungus was collected from 15 rice cultivars at nine locations during 2000-05 and characterised using Pot2-DNA fingerprinting. Based on DNA analysis (isolates with ≥70% band similarity), five lineages were identified and designated A, B, C, D and E, with 11, 22, 4, 1 and 4 haplotypes identified, respectively. The predominant lineage, B, representing 38% of the collected isolates, was recovered from four cultivars in five locations. In contrast to lineages A and B, which did not contain a dominant haplotype, a single haplotype predominated in lineages C and E. Isolates representing all haplotypes were examined for virulence on a set of differential rice cultivars, near-isogenic lines and commercial cultivars commonly grown in Argentina, revealing 41 pathotypes and 24 international races. There was no significant association between DNA fingerprint similarities and pathotypes. Overall, these data indicated that populations of <i>P. oryzae</i> in Argentina are genetically simple and predominantly clonal yet have a high pathotype diversity.Centro de Investigaciones en Fitopatología2008-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf357-364http://sedici.unlp.edu.ar/handle/10915/144408enginfo:eu-repo/semantics/altIdentifier/issn/0815-3191info:eu-repo/semantics/altIdentifier/issn/1448-6032info:eu-repo/semantics/altIdentifier/issn/0310-1266info:eu-repo/semantics/altIdentifier/doi/10.1071/ap08010info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:32Zoai:sedici.unlp.edu.ar:10915/144408Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:33.205SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv DNA fingerprint and pathotype diversity of Pyricularia oryzae populations from Argentina
title DNA fingerprint and pathotype diversity of Pyricularia oryzae populations from Argentina
spellingShingle DNA fingerprint and pathotype diversity of Pyricularia oryzae populations from Argentina
Consolo, Verónica Fabiana
Ciencias Agrarias
Magnaporthe oryzae
population structure
rice breeding
title_short DNA fingerprint and pathotype diversity of Pyricularia oryzae populations from Argentina
title_full DNA fingerprint and pathotype diversity of Pyricularia oryzae populations from Argentina
title_fullStr DNA fingerprint and pathotype diversity of Pyricularia oryzae populations from Argentina
title_full_unstemmed DNA fingerprint and pathotype diversity of Pyricularia oryzae populations from Argentina
title_sort DNA fingerprint and pathotype diversity of Pyricularia oryzae populations from Argentina
dc.creator.none.fl_str_mv Consolo, Verónica Fabiana
Cordo, Cristina Alicia
Salerno, Graciela Lidia
author Consolo, Verónica Fabiana
author_facet Consolo, Verónica Fabiana
Cordo, Cristina Alicia
Salerno, Graciela Lidia
author_role author
author2 Cordo, Cristina Alicia
Salerno, Graciela Lidia
author2_role author
author
dc.subject.none.fl_str_mv Ciencias Agrarias
Magnaporthe oryzae
population structure
rice breeding
topic Ciencias Agrarias
Magnaporthe oryzae
population structure
rice breeding
dc.description.none.fl_txt_mv The genetic diversity of the rice blast pathogen, <i>Pyricularia oryzae</i>, was analysed in rice-growing provinces of Argentina. A total of 161 isolates of the fungus was collected from 15 rice cultivars at nine locations during 2000-05 and characterised using Pot2-DNA fingerprinting. Based on DNA analysis (isolates with ≥70% band similarity), five lineages were identified and designated A, B, C, D and E, with 11, 22, 4, 1 and 4 haplotypes identified, respectively. The predominant lineage, B, representing 38% of the collected isolates, was recovered from four cultivars in five locations. In contrast to lineages A and B, which did not contain a dominant haplotype, a single haplotype predominated in lineages C and E. Isolates representing all haplotypes were examined for virulence on a set of differential rice cultivars, near-isogenic lines and commercial cultivars commonly grown in Argentina, revealing 41 pathotypes and 24 international races. There was no significant association between DNA fingerprint similarities and pathotypes. Overall, these data indicated that populations of <i>P. oryzae</i> in Argentina are genetically simple and predominantly clonal yet have a high pathotype diversity.
Centro de Investigaciones en Fitopatología
description The genetic diversity of the rice blast pathogen, <i>Pyricularia oryzae</i>, was analysed in rice-growing provinces of Argentina. A total of 161 isolates of the fungus was collected from 15 rice cultivars at nine locations during 2000-05 and characterised using Pot2-DNA fingerprinting. Based on DNA analysis (isolates with ≥70% band similarity), five lineages were identified and designated A, B, C, D and E, with 11, 22, 4, 1 and 4 haplotypes identified, respectively. The predominant lineage, B, representing 38% of the collected isolates, was recovered from four cultivars in five locations. In contrast to lineages A and B, which did not contain a dominant haplotype, a single haplotype predominated in lineages C and E. Isolates representing all haplotypes were examined for virulence on a set of differential rice cultivars, near-isogenic lines and commercial cultivars commonly grown in Argentina, revealing 41 pathotypes and 24 international races. There was no significant association between DNA fingerprint similarities and pathotypes. Overall, these data indicated that populations of <i>P. oryzae</i> in Argentina are genetically simple and predominantly clonal yet have a high pathotype diversity.
publishDate 2008
dc.date.none.fl_str_mv 2008-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/144408
url http://sedici.unlp.edu.ar/handle/10915/144408
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0815-3191
info:eu-repo/semantics/altIdentifier/issn/1448-6032
info:eu-repo/semantics/altIdentifier/issn/0310-1266
info:eu-repo/semantics/altIdentifier/doi/10.1071/ap08010
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
357-364
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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