Influence of calcitonin gene-related peptide release on pH-induced mechanical depression in rat atria

Autores
Gómez Alvis, Alicia; Milesi, María Verónica; Rebolledo, Alejandro; Raingo, Jesica; Grassi de Gende, Ángela Ofelia
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rat atria is richly innervated by sensory nerve fibers that release CGRP when stimulated either by capsaicin or acid pH. We studied the physiological relevance of acid pH-induced CGRP release on changes in atrial contractility and relaxation produced by lowering the pH. Isolated atria electrically paced at 2.77 Hz were exposed to a 10-minute period of metabolic acidosis (pH=6.73±0.01, n=28) after: 1) CGRP release induced by capsaicin 0.5 μM; 2) blockage of CGRP release with ruthenium red (RR) 5 μM; 3) no pretreatment; and 4) CGRP receptor blockage with CGRP8-37 1 μM. Contractility and relaxation were significantly less depressed by acid pH when CGRP release was prevented by RR or CGRP receptor activation was blocked by CGRP8-37. The results suggest that CGRP release and the activation of CGRP receptors may be physiologically involved in contributing to the depression of contractility and relaxation induced by acid pH in rat atria.
Facultad de Ciencias Exactas
Materia
Ciencias Exactas
Acid pH
Capsaicin
CGRP receptor blockage
Rat atria
Ruthenium red
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/82983
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/82983
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Influence of calcitonin gene-related peptide release on pH-induced mechanical depression in rat atriaGómez Alvis, AliciaMilesi, María VerónicaRebolledo, AlejandroRaingo, JesicaGrassi de Gende, Ángela OfeliaCiencias ExactasAcid pHCapsaicinCGRP receptor blockageRat atriaRuthenium redRat atria is richly innervated by sensory nerve fibers that release CGRP when stimulated either by capsaicin or acid pH. We studied the physiological relevance of acid pH-induced CGRP release on changes in atrial contractility and relaxation produced by lowering the pH. Isolated atria electrically paced at 2.77 Hz were exposed to a 10-minute period of metabolic acidosis (pH=6.73±0.01, n=28) after: 1) CGRP release induced by capsaicin 0.5 μM; 2) blockage of CGRP release with ruthenium red (RR) 5 μM; 3) no pretreatment; and 4) CGRP receptor blockage with CGRP<SUB>8-37</SUB> 1 μM. Contractility and relaxation were significantly less depressed by acid pH when CGRP release was prevented by RR or CGRP receptor activation was blocked by CGRP<SUB>8-37</SUB>. The results suggest that CGRP release and the activation of CGRP receptors may be physiologically involved in contributing to the depression of contractility and relaxation induced by acid pH in rat atria.Facultad de Ciencias Exactas2001info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf507-517http://sedici.unlp.edu.ar/handle/10915/82983enginfo:eu-repo/semantics/altIdentifier/issn/0021-4868info:eu-repo/semantics/altIdentifier/doi/10.1536/jhj.42.507info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:15:50Zoai:sedici.unlp.edu.ar:10915/82983Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:15:50.933SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Influence of calcitonin gene-related peptide release on pH-induced mechanical depression in rat atria
title Influence of calcitonin gene-related peptide release on pH-induced mechanical depression in rat atria
spellingShingle Influence of calcitonin gene-related peptide release on pH-induced mechanical depression in rat atria
Gómez Alvis, Alicia
Ciencias Exactas
Acid pH
Capsaicin
CGRP receptor blockage
Rat atria
Ruthenium red
title_short Influence of calcitonin gene-related peptide release on pH-induced mechanical depression in rat atria
title_full Influence of calcitonin gene-related peptide release on pH-induced mechanical depression in rat atria
title_fullStr Influence of calcitonin gene-related peptide release on pH-induced mechanical depression in rat atria
title_full_unstemmed Influence of calcitonin gene-related peptide release on pH-induced mechanical depression in rat atria
title_sort Influence of calcitonin gene-related peptide release on pH-induced mechanical depression in rat atria
dc.creator.none.fl_str_mv Gómez Alvis, Alicia
Milesi, María Verónica
Rebolledo, Alejandro
Raingo, Jesica
Grassi de Gende, Ángela Ofelia
author Gómez Alvis, Alicia
author_facet Gómez Alvis, Alicia
Milesi, María Verónica
Rebolledo, Alejandro
Raingo, Jesica
Grassi de Gende, Ángela Ofelia
author_role author
author2 Milesi, María Verónica
Rebolledo, Alejandro
Raingo, Jesica
Grassi de Gende, Ángela Ofelia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Acid pH
Capsaicin
CGRP receptor blockage
Rat atria
Ruthenium red
topic Ciencias Exactas
Acid pH
Capsaicin
CGRP receptor blockage
Rat atria
Ruthenium red
dc.description.none.fl_txt_mv Rat atria is richly innervated by sensory nerve fibers that release CGRP when stimulated either by capsaicin or acid pH. We studied the physiological relevance of acid pH-induced CGRP release on changes in atrial contractility and relaxation produced by lowering the pH. Isolated atria electrically paced at 2.77 Hz were exposed to a 10-minute period of metabolic acidosis (pH=6.73±0.01, n=28) after: 1) CGRP release induced by capsaicin 0.5 μM; 2) blockage of CGRP release with ruthenium red (RR) 5 μM; 3) no pretreatment; and 4) CGRP receptor blockage with CGRP<SUB>8-37</SUB> 1 μM. Contractility and relaxation were significantly less depressed by acid pH when CGRP release was prevented by RR or CGRP receptor activation was blocked by CGRP<SUB>8-37</SUB>. The results suggest that CGRP release and the activation of CGRP receptors may be physiologically involved in contributing to the depression of contractility and relaxation induced by acid pH in rat atria.
Facultad de Ciencias Exactas
description Rat atria is richly innervated by sensory nerve fibers that release CGRP when stimulated either by capsaicin or acid pH. We studied the physiological relevance of acid pH-induced CGRP release on changes in atrial contractility and relaxation produced by lowering the pH. Isolated atria electrically paced at 2.77 Hz were exposed to a 10-minute period of metabolic acidosis (pH=6.73±0.01, n=28) after: 1) CGRP release induced by capsaicin 0.5 μM; 2) blockage of CGRP release with ruthenium red (RR) 5 μM; 3) no pretreatment; and 4) CGRP receptor blockage with CGRP<SUB>8-37</SUB> 1 μM. Contractility and relaxation were significantly less depressed by acid pH when CGRP release was prevented by RR or CGRP receptor activation was blocked by CGRP<SUB>8-37</SUB>. The results suggest that CGRP release and the activation of CGRP receptors may be physiologically involved in contributing to the depression of contractility and relaxation induced by acid pH in rat atria.
publishDate 2001
dc.date.none.fl_str_mv 2001
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/82983
url http://sedici.unlp.edu.ar/handle/10915/82983
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0021-4868
info:eu-repo/semantics/altIdentifier/doi/10.1536/jhj.42.507
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
507-517
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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