Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study

Autores
Vázquez, Diego E.; Latorre Estivalis, José Manuel; Ons, Sheila; Farina, Walter M.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The honey bee Apis mellifera is the most abundant managed pollinator in diverse crops worldwide. Consequently, it is exposed to a plethora of environmental stressors, among which are the agrochemicals. In agroecosystems, the herbicide glyphosate (GLY) is one of the most applied. In laboratory assessments, GLY affects the honey bee larval development by delaying its moulting, among other negative effects. However, it is still unknown how GLY affects larval physiology when there are no observable signs of toxicity. We carried out a longitudinal experimental design using the in vitro rearing procedure. Larvae were fed with food containing or not a sub-lethal dose of GLY in chronic exposure (120 h). Individuals without observable signs of toxicity were sampled and their gene expression profile was analyzed with a transcriptomic approach to compare between treatments. Even though 29% of larvae were asymptomatic in the exposed group, they showed transcriptional changes in several genes after the GLY chronic intake. A total of 19 transcripts were found to be differentially expressed in the RNA-Seq experiment, mainly linked with defensive response and intermediary metabolism processes. Furthermore, the enriched functional categories in the transcriptome of the exposed asymptomatic larvae were linked with enzymes with catalytic and redox activity. Our results suggest an enhanced catabolism and oxidative metabolism in honey bee larvae as a consequence of the sub-lethal exposure to GLY, even in the absence of observable symptoms.
Centro Regional de Estudios Genómicos
Materia
Ciencias Exactas
Biología
Apis mellifera
RNA-Seq
in vitro rearing
transcriptomic
energy metabolism
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/3.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/139120

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network_name_str SEDICI (UNLP)
spelling Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic studyVázquez, Diego E.Latorre Estivalis, José ManuelOns, SheilaFarina, Walter M.Ciencias ExactasBiologíaApis melliferaRNA-Seqin vitro rearingtranscriptomicenergy metabolismThe honey bee Apis mellifera is the most abundant managed pollinator in diverse crops worldwide. Consequently, it is exposed to a plethora of environmental stressors, among which are the agrochemicals. In agroecosystems, the herbicide glyphosate (GLY) is one of the most applied. In laboratory assessments, GLY affects the honey bee larval development by delaying its moulting, among other negative effects. However, it is still unknown how GLY affects larval physiology when there are no observable signs of toxicity. We carried out a longitudinal experimental design using the in vitro rearing procedure. Larvae were fed with food containing or not a sub-lethal dose of GLY in chronic exposure (120 h). Individuals without observable signs of toxicity were sampled and their gene expression profile was analyzed with a transcriptomic approach to compare between treatments. Even though 29% of larvae were asymptomatic in the exposed group, they showed transcriptional changes in several genes after the GLY chronic intake. A total of 19 transcripts were found to be differentially expressed in the RNA-Seq experiment, mainly linked with defensive response and intermediary metabolism processes. Furthermore, the enriched functional categories in the transcriptome of the exposed asymptomatic larvae were linked with enzymes with catalytic and redox activity. Our results suggest an enhanced catabolism and oxidative metabolism in honey bee larvae as a consequence of the sub-lethal exposure to GLY, even in the absence of observable symptoms.Centro Regional de Estudios Genómicos2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf114148-114148http://sedici.unlp.edu.ar/handle/10915/139120enginfo:eu-repo/semantics/altIdentifier/issn/1873-6424info:eu-repo/semantics/altIdentifier/issn/0269-7491info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envpol.2020.114148info:eu-repo/semantics/altIdentifier/pmid/32062465info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/3.0/Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:31:57Zoai:sedici.unlp.edu.ar:10915/139120Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:31:57.27SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study
title Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study
spellingShingle Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study
Vázquez, Diego E.
Ciencias Exactas
Biología
Apis mellifera
RNA-Seq
in vitro rearing
transcriptomic
energy metabolism
title_short Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study
title_full Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study
title_fullStr Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study
title_full_unstemmed Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study
title_sort Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study
dc.creator.none.fl_str_mv Vázquez, Diego E.
Latorre Estivalis, José Manuel
Ons, Sheila
Farina, Walter M.
author Vázquez, Diego E.
author_facet Vázquez, Diego E.
Latorre Estivalis, José Manuel
Ons, Sheila
Farina, Walter M.
author_role author
author2 Latorre Estivalis, José Manuel
Ons, Sheila
Farina, Walter M.
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Biología
Apis mellifera
RNA-Seq
in vitro rearing
transcriptomic
energy metabolism
topic Ciencias Exactas
Biología
Apis mellifera
RNA-Seq
in vitro rearing
transcriptomic
energy metabolism
dc.description.none.fl_txt_mv The honey bee Apis mellifera is the most abundant managed pollinator in diverse crops worldwide. Consequently, it is exposed to a plethora of environmental stressors, among which are the agrochemicals. In agroecosystems, the herbicide glyphosate (GLY) is one of the most applied. In laboratory assessments, GLY affects the honey bee larval development by delaying its moulting, among other negative effects. However, it is still unknown how GLY affects larval physiology when there are no observable signs of toxicity. We carried out a longitudinal experimental design using the in vitro rearing procedure. Larvae were fed with food containing or not a sub-lethal dose of GLY in chronic exposure (120 h). Individuals without observable signs of toxicity were sampled and their gene expression profile was analyzed with a transcriptomic approach to compare between treatments. Even though 29% of larvae were asymptomatic in the exposed group, they showed transcriptional changes in several genes after the GLY chronic intake. A total of 19 transcripts were found to be differentially expressed in the RNA-Seq experiment, mainly linked with defensive response and intermediary metabolism processes. Furthermore, the enriched functional categories in the transcriptome of the exposed asymptomatic larvae were linked with enzymes with catalytic and redox activity. Our results suggest an enhanced catabolism and oxidative metabolism in honey bee larvae as a consequence of the sub-lethal exposure to GLY, even in the absence of observable symptoms.
Centro Regional de Estudios Genómicos
description The honey bee Apis mellifera is the most abundant managed pollinator in diverse crops worldwide. Consequently, it is exposed to a plethora of environmental stressors, among which are the agrochemicals. In agroecosystems, the herbicide glyphosate (GLY) is one of the most applied. In laboratory assessments, GLY affects the honey bee larval development by delaying its moulting, among other negative effects. However, it is still unknown how GLY affects larval physiology when there are no observable signs of toxicity. We carried out a longitudinal experimental design using the in vitro rearing procedure. Larvae were fed with food containing or not a sub-lethal dose of GLY in chronic exposure (120 h). Individuals without observable signs of toxicity were sampled and their gene expression profile was analyzed with a transcriptomic approach to compare between treatments. Even though 29% of larvae were asymptomatic in the exposed group, they showed transcriptional changes in several genes after the GLY chronic intake. A total of 19 transcripts were found to be differentially expressed in the RNA-Seq experiment, mainly linked with defensive response and intermediary metabolism processes. Furthermore, the enriched functional categories in the transcriptome of the exposed asymptomatic larvae were linked with enzymes with catalytic and redox activity. Our results suggest an enhanced catabolism and oxidative metabolism in honey bee larvae as a consequence of the sub-lethal exposure to GLY, even in the absence of observable symptoms.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/139120
url http://sedici.unlp.edu.ar/handle/10915/139120
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1873-6424
info:eu-repo/semantics/altIdentifier/issn/0269-7491
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envpol.2020.114148
info:eu-repo/semantics/altIdentifier/pmid/32062465
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/3.0/
Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/3.0/
Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0)
dc.format.none.fl_str_mv application/pdf
114148-114148
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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