Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study
- Autores
- Vázquez, Diego E.; Latorre Estivalis, José Manuel; Ons, Sheila; Farina, Walter M.
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The honey bee Apis mellifera is the most abundant managed pollinator in diverse crops worldwide. Consequently, it is exposed to a plethora of environmental stressors, among which are the agrochemicals. In agroecosystems, the herbicide glyphosate (GLY) is one of the most applied. In laboratory assessments, GLY affects the honey bee larval development by delaying its moulting, among other negative effects. However, it is still unknown how GLY affects larval physiology when there are no observable signs of toxicity. We carried out a longitudinal experimental design using the in vitro rearing procedure. Larvae were fed with food containing or not a sub-lethal dose of GLY in chronic exposure (120 h). Individuals without observable signs of toxicity were sampled and their gene expression profile was analyzed with a transcriptomic approach to compare between treatments. Even though 29% of larvae were asymptomatic in the exposed group, they showed transcriptional changes in several genes after the GLY chronic intake. A total of 19 transcripts were found to be differentially expressed in the RNA-Seq experiment, mainly linked with defensive response and intermediary metabolism processes. Furthermore, the enriched functional categories in the transcriptome of the exposed asymptomatic larvae were linked with enzymes with catalytic and redox activity. Our results suggest an enhanced catabolism and oxidative metabolism in honey bee larvae as a consequence of the sub-lethal exposure to GLY, even in the absence of observable symptoms.
Centro Regional de Estudios Genómicos - Materia
-
Ciencias Exactas
Biología
Apis mellifera
RNA-Seq
in vitro rearing
transcriptomic
energy metabolism - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/3.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/139120
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Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic studyVázquez, Diego E.Latorre Estivalis, José ManuelOns, SheilaFarina, Walter M.Ciencias ExactasBiologíaApis melliferaRNA-Seqin vitro rearingtranscriptomicenergy metabolismThe honey bee Apis mellifera is the most abundant managed pollinator in diverse crops worldwide. Consequently, it is exposed to a plethora of environmental stressors, among which are the agrochemicals. In agroecosystems, the herbicide glyphosate (GLY) is one of the most applied. In laboratory assessments, GLY affects the honey bee larval development by delaying its moulting, among other negative effects. However, it is still unknown how GLY affects larval physiology when there are no observable signs of toxicity. We carried out a longitudinal experimental design using the in vitro rearing procedure. Larvae were fed with food containing or not a sub-lethal dose of GLY in chronic exposure (120 h). Individuals without observable signs of toxicity were sampled and their gene expression profile was analyzed with a transcriptomic approach to compare between treatments. Even though 29% of larvae were asymptomatic in the exposed group, they showed transcriptional changes in several genes after the GLY chronic intake. A total of 19 transcripts were found to be differentially expressed in the RNA-Seq experiment, mainly linked with defensive response and intermediary metabolism processes. Furthermore, the enriched functional categories in the transcriptome of the exposed asymptomatic larvae were linked with enzymes with catalytic and redox activity. Our results suggest an enhanced catabolism and oxidative metabolism in honey bee larvae as a consequence of the sub-lethal exposure to GLY, even in the absence of observable symptoms.Centro Regional de Estudios Genómicos2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf114148-114148http://sedici.unlp.edu.ar/handle/10915/139120enginfo:eu-repo/semantics/altIdentifier/issn/1873-6424info:eu-repo/semantics/altIdentifier/issn/0269-7491info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envpol.2020.114148info:eu-repo/semantics/altIdentifier/pmid/32062465info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/3.0/Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:31:57Zoai:sedici.unlp.edu.ar:10915/139120Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:31:57.27SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study |
title |
Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study |
spellingShingle |
Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study Vázquez, Diego E. Ciencias Exactas Biología Apis mellifera RNA-Seq in vitro rearing transcriptomic energy metabolism |
title_short |
Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study |
title_full |
Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study |
title_fullStr |
Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study |
title_full_unstemmed |
Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study |
title_sort |
Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study |
dc.creator.none.fl_str_mv |
Vázquez, Diego E. Latorre Estivalis, José Manuel Ons, Sheila Farina, Walter M. |
author |
Vázquez, Diego E. |
author_facet |
Vázquez, Diego E. Latorre Estivalis, José Manuel Ons, Sheila Farina, Walter M. |
author_role |
author |
author2 |
Latorre Estivalis, José Manuel Ons, Sheila Farina, Walter M. |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Ciencias Exactas Biología Apis mellifera RNA-Seq in vitro rearing transcriptomic energy metabolism |
topic |
Ciencias Exactas Biología Apis mellifera RNA-Seq in vitro rearing transcriptomic energy metabolism |
dc.description.none.fl_txt_mv |
The honey bee Apis mellifera is the most abundant managed pollinator in diverse crops worldwide. Consequently, it is exposed to a plethora of environmental stressors, among which are the agrochemicals. In agroecosystems, the herbicide glyphosate (GLY) is one of the most applied. In laboratory assessments, GLY affects the honey bee larval development by delaying its moulting, among other negative effects. However, it is still unknown how GLY affects larval physiology when there are no observable signs of toxicity. We carried out a longitudinal experimental design using the in vitro rearing procedure. Larvae were fed with food containing or not a sub-lethal dose of GLY in chronic exposure (120 h). Individuals without observable signs of toxicity were sampled and their gene expression profile was analyzed with a transcriptomic approach to compare between treatments. Even though 29% of larvae were asymptomatic in the exposed group, they showed transcriptional changes in several genes after the GLY chronic intake. A total of 19 transcripts were found to be differentially expressed in the RNA-Seq experiment, mainly linked with defensive response and intermediary metabolism processes. Furthermore, the enriched functional categories in the transcriptome of the exposed asymptomatic larvae were linked with enzymes with catalytic and redox activity. Our results suggest an enhanced catabolism and oxidative metabolism in honey bee larvae as a consequence of the sub-lethal exposure to GLY, even in the absence of observable symptoms. Centro Regional de Estudios Genómicos |
description |
The honey bee Apis mellifera is the most abundant managed pollinator in diverse crops worldwide. Consequently, it is exposed to a plethora of environmental stressors, among which are the agrochemicals. In agroecosystems, the herbicide glyphosate (GLY) is one of the most applied. In laboratory assessments, GLY affects the honey bee larval development by delaying its moulting, among other negative effects. However, it is still unknown how GLY affects larval physiology when there are no observable signs of toxicity. We carried out a longitudinal experimental design using the in vitro rearing procedure. Larvae were fed with food containing or not a sub-lethal dose of GLY in chronic exposure (120 h). Individuals without observable signs of toxicity were sampled and their gene expression profile was analyzed with a transcriptomic approach to compare between treatments. Even though 29% of larvae were asymptomatic in the exposed group, they showed transcriptional changes in several genes after the GLY chronic intake. A total of 19 transcripts were found to be differentially expressed in the RNA-Seq experiment, mainly linked with defensive response and intermediary metabolism processes. Furthermore, the enriched functional categories in the transcriptome of the exposed asymptomatic larvae were linked with enzymes with catalytic and redox activity. Our results suggest an enhanced catabolism and oxidative metabolism in honey bee larvae as a consequence of the sub-lethal exposure to GLY, even in the absence of observable symptoms. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/139120 |
url |
http://sedici.unlp.edu.ar/handle/10915/139120 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/1873-6424 info:eu-repo/semantics/altIdentifier/issn/0269-7491 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envpol.2020.114148 info:eu-repo/semantics/altIdentifier/pmid/32062465 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/3.0/ Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0) |
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openAccess |
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http://creativecommons.org/licenses/by-nc-sa/3.0/ Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0) |
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