Are the damaging effects induced by the imazethapyr formulation Pivot® H in <i>Boana pulchella</i> (Anura) reversible upon ceasing exposure?

Autores
Pérez Iglesias, Juan Manuel; Natale, Guillermo Sebastián; Soloneski, Sonia María Elsa; Larramendy, Marcelo Luis
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present study, the damage recovery capabilities of Boana pulchella tadpoles after acute exposure (96 h) to 0.39 mg/L concentration of the imazethapyr (IMZT)-based herbicide formulation Pivot® H (25% IMZT LC50 value) were assessed during a period of 7 to −21 days. To appraise the recovery capabilities, frequency of micronuclei (MNs), other nuclear abnormalities and DNA single-strand breaks evaluated by single cell gel electrophoresis assay on circulating blood cells were employed as endpoints for genotoxicity. Growth, development, body mass, and morphological abnormalities were also employed as individual endpoints in the recovery assay. Results demonstrated that IMZT induced sublethal effects at both the individual (i.e., loss of keratodonts) and cytogenetic levels (e.g., increase of MN frequency, other nuclear abnormalities and DNA single-strand breaks). At 11 days of the exposure phase, tadpoles recovered their basal levels of frequency of MNs, other nuclear abnormalities, and comets. However, loss of keratodonts, observed at the end of the exposure period, was present up to 21 days thereafter. Finally, axial abnormalities and delay in development stage were observed only during the postexposure phase in IMZT-exposed tadpoles at 18 and 25 days, respectively and were observed until the end of the experiment. This is the first evidence of use the comet assay as cytogenetic biomarker of genotoxicity in evaluating the recovery capabilities of amphibians in general and also those of B. pulchella after exposure to IMZT.
Facultad de Ciencias Naturales y Museo
Facultad de Ciencias Exactas
Centro de Investigaciones del Medioambiente
Materia
Ciencias Naturales
Ciencias Exactas
imazethapyr
Pivot® H
recovery capabilities
comet assay
micronuclei
morphological abnormalities
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/109182

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network_name_str SEDICI (UNLP)
spelling Are the damaging effects induced by the imazethapyr formulation Pivot® H in <i>Boana pulchella</i> (Anura) reversible upon ceasing exposure?Pérez Iglesias, Juan ManuelNatale, Guillermo SebastiánSoloneski, Sonia María ElsaLarramendy, Marcelo LuisCiencias NaturalesCiencias ExactasimazethapyrPivot® Hrecovery capabilitiescomet assaymicronucleimorphological abnormalitiesIn the present study, the damage recovery capabilities of <i>Boana pulchella</i> tadpoles after acute exposure (96 h) to 0.39 mg/L concentration of the imazethapyr (IMZT)-based herbicide formulation Pivot® H (25% IMZT LC<sub>50</sub> value) were assessed during a period of 7 to −21 days. To appraise the recovery capabilities, frequency of micronuclei (MNs), other nuclear abnormalities and DNA single-strand breaks evaluated by single cell gel electrophoresis assay on circulating blood cells were employed as endpoints for genotoxicity. Growth, development, body mass, and morphological abnormalities were also employed as individual endpoints in the recovery assay. Results demonstrated that IMZT induced sublethal effects at both the individual (i.e., loss of keratodonts) and cytogenetic levels (e.g., increase of MN frequency, other nuclear abnormalities and DNA single-strand breaks). At 11 days of the exposure phase, tadpoles recovered their basal levels of frequency of MNs, other nuclear abnormalities, and comets. However, loss of keratodonts, observed at the end of the exposure period, was present up to 21 days thereafter. Finally, axial abnormalities and delay in development stage were observed only during the postexposure phase in IMZT-exposed tadpoles at 18 and 25 days, respectively and were observed until the end of the experiment. This is the first evidence of use the comet assay as cytogenetic biomarker of genotoxicity in evaluating the recovery capabilities of amphibians in general and also those of <i>B. pulchella</i> after exposure to IMZT.Facultad de Ciencias Naturales y MuseoFacultad de Ciencias ExactasCentro de Investigaciones del Medioambiente2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1-10http://sedici.unlp.edu.ar/handle/10915/109182enginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0147651317306814info:eu-repo/semantics/altIdentifier/issn/0147-6513info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2017.10.009info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:24:42Zoai:sedici.unlp.edu.ar:10915/109182Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:24:43.234SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Are the damaging effects induced by the imazethapyr formulation Pivot® H in <i>Boana pulchella</i> (Anura) reversible upon ceasing exposure?
title Are the damaging effects induced by the imazethapyr formulation Pivot® H in <i>Boana pulchella</i> (Anura) reversible upon ceasing exposure?
spellingShingle Are the damaging effects induced by the imazethapyr formulation Pivot® H in <i>Boana pulchella</i> (Anura) reversible upon ceasing exposure?
Pérez Iglesias, Juan Manuel
Ciencias Naturales
Ciencias Exactas
imazethapyr
Pivot® H
recovery capabilities
comet assay
micronuclei
morphological abnormalities
title_short Are the damaging effects induced by the imazethapyr formulation Pivot® H in <i>Boana pulchella</i> (Anura) reversible upon ceasing exposure?
title_full Are the damaging effects induced by the imazethapyr formulation Pivot® H in <i>Boana pulchella</i> (Anura) reversible upon ceasing exposure?
title_fullStr Are the damaging effects induced by the imazethapyr formulation Pivot® H in <i>Boana pulchella</i> (Anura) reversible upon ceasing exposure?
title_full_unstemmed Are the damaging effects induced by the imazethapyr formulation Pivot® H in <i>Boana pulchella</i> (Anura) reversible upon ceasing exposure?
title_sort Are the damaging effects induced by the imazethapyr formulation Pivot® H in <i>Boana pulchella</i> (Anura) reversible upon ceasing exposure?
dc.creator.none.fl_str_mv Pérez Iglesias, Juan Manuel
Natale, Guillermo Sebastián
Soloneski, Sonia María Elsa
Larramendy, Marcelo Luis
author Pérez Iglesias, Juan Manuel
author_facet Pérez Iglesias, Juan Manuel
Natale, Guillermo Sebastián
Soloneski, Sonia María Elsa
Larramendy, Marcelo Luis
author_role author
author2 Natale, Guillermo Sebastián
Soloneski, Sonia María Elsa
Larramendy, Marcelo Luis
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Naturales
Ciencias Exactas
imazethapyr
Pivot® H
recovery capabilities
comet assay
micronuclei
morphological abnormalities
topic Ciencias Naturales
Ciencias Exactas
imazethapyr
Pivot® H
recovery capabilities
comet assay
micronuclei
morphological abnormalities
dc.description.none.fl_txt_mv In the present study, the damage recovery capabilities of <i>Boana pulchella</i> tadpoles after acute exposure (96 h) to 0.39 mg/L concentration of the imazethapyr (IMZT)-based herbicide formulation Pivot® H (25% IMZT LC<sub>50</sub> value) were assessed during a period of 7 to −21 days. To appraise the recovery capabilities, frequency of micronuclei (MNs), other nuclear abnormalities and DNA single-strand breaks evaluated by single cell gel electrophoresis assay on circulating blood cells were employed as endpoints for genotoxicity. Growth, development, body mass, and morphological abnormalities were also employed as individual endpoints in the recovery assay. Results demonstrated that IMZT induced sublethal effects at both the individual (i.e., loss of keratodonts) and cytogenetic levels (e.g., increase of MN frequency, other nuclear abnormalities and DNA single-strand breaks). At 11 days of the exposure phase, tadpoles recovered their basal levels of frequency of MNs, other nuclear abnormalities, and comets. However, loss of keratodonts, observed at the end of the exposure period, was present up to 21 days thereafter. Finally, axial abnormalities and delay in development stage were observed only during the postexposure phase in IMZT-exposed tadpoles at 18 and 25 days, respectively and were observed until the end of the experiment. This is the first evidence of use the comet assay as cytogenetic biomarker of genotoxicity in evaluating the recovery capabilities of amphibians in general and also those of <i>B. pulchella</i> after exposure to IMZT.
Facultad de Ciencias Naturales y Museo
Facultad de Ciencias Exactas
Centro de Investigaciones del Medioambiente
description In the present study, the damage recovery capabilities of <i>Boana pulchella</i> tadpoles after acute exposure (96 h) to 0.39 mg/L concentration of the imazethapyr (IMZT)-based herbicide formulation Pivot® H (25% IMZT LC<sub>50</sub> value) were assessed during a period of 7 to −21 days. To appraise the recovery capabilities, frequency of micronuclei (MNs), other nuclear abnormalities and DNA single-strand breaks evaluated by single cell gel electrophoresis assay on circulating blood cells were employed as endpoints for genotoxicity. Growth, development, body mass, and morphological abnormalities were also employed as individual endpoints in the recovery assay. Results demonstrated that IMZT induced sublethal effects at both the individual (i.e., loss of keratodonts) and cytogenetic levels (e.g., increase of MN frequency, other nuclear abnormalities and DNA single-strand breaks). At 11 days of the exposure phase, tadpoles recovered their basal levels of frequency of MNs, other nuclear abnormalities, and comets. However, loss of keratodonts, observed at the end of the exposure period, was present up to 21 days thereafter. Finally, axial abnormalities and delay in development stage were observed only during the postexposure phase in IMZT-exposed tadpoles at 18 and 25 days, respectively and were observed until the end of the experiment. This is the first evidence of use the comet assay as cytogenetic biomarker of genotoxicity in evaluating the recovery capabilities of amphibians in general and also those of <i>B. pulchella</i> after exposure to IMZT.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/109182
url http://sedici.unlp.edu.ar/handle/10915/109182
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dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0147651317306814
info:eu-repo/semantics/altIdentifier/issn/0147-6513
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2017.10.009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
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