Are the damaging effects induced by the imazethapyr formulation Pivot® H in <i>Boana pulchella</i> (Anura) reversible upon ceasing exposure?
- Autores
- Pérez Iglesias, Juan Manuel; Natale, Guillermo Sebastián; Soloneski, Sonia María Elsa; Larramendy, Marcelo Luis
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In the present study, the damage recovery capabilities of Boana pulchella tadpoles after acute exposure (96 h) to 0.39 mg/L concentration of the imazethapyr (IMZT)-based herbicide formulation Pivot® H (25% IMZT LC50 value) were assessed during a period of 7 to −21 days. To appraise the recovery capabilities, frequency of micronuclei (MNs), other nuclear abnormalities and DNA single-strand breaks evaluated by single cell gel electrophoresis assay on circulating blood cells were employed as endpoints for genotoxicity. Growth, development, body mass, and morphological abnormalities were also employed as individual endpoints in the recovery assay. Results demonstrated that IMZT induced sublethal effects at both the individual (i.e., loss of keratodonts) and cytogenetic levels (e.g., increase of MN frequency, other nuclear abnormalities and DNA single-strand breaks). At 11 days of the exposure phase, tadpoles recovered their basal levels of frequency of MNs, other nuclear abnormalities, and comets. However, loss of keratodonts, observed at the end of the exposure period, was present up to 21 days thereafter. Finally, axial abnormalities and delay in development stage were observed only during the postexposure phase in IMZT-exposed tadpoles at 18 and 25 days, respectively and were observed until the end of the experiment. This is the first evidence of use the comet assay as cytogenetic biomarker of genotoxicity in evaluating the recovery capabilities of amphibians in general and also those of B. pulchella after exposure to IMZT.
Facultad de Ciencias Naturales y Museo
Facultad de Ciencias Exactas
Centro de Investigaciones del Medioambiente - Materia
-
Ciencias Naturales
Ciencias Exactas
imazethapyr
Pivot® H
recovery capabilities
comet assay
micronuclei
morphological abnormalities - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/109182
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Are the damaging effects induced by the imazethapyr formulation Pivot® H in <i>Boana pulchella</i> (Anura) reversible upon ceasing exposure?Pérez Iglesias, Juan ManuelNatale, Guillermo SebastiánSoloneski, Sonia María ElsaLarramendy, Marcelo LuisCiencias NaturalesCiencias ExactasimazethapyrPivot® Hrecovery capabilitiescomet assaymicronucleimorphological abnormalitiesIn the present study, the damage recovery capabilities of <i>Boana pulchella</i> tadpoles after acute exposure (96 h) to 0.39 mg/L concentration of the imazethapyr (IMZT)-based herbicide formulation Pivot® H (25% IMZT LC<sub>50</sub> value) were assessed during a period of 7 to −21 days. To appraise the recovery capabilities, frequency of micronuclei (MNs), other nuclear abnormalities and DNA single-strand breaks evaluated by single cell gel electrophoresis assay on circulating blood cells were employed as endpoints for genotoxicity. Growth, development, body mass, and morphological abnormalities were also employed as individual endpoints in the recovery assay. Results demonstrated that IMZT induced sublethal effects at both the individual (i.e., loss of keratodonts) and cytogenetic levels (e.g., increase of MN frequency, other nuclear abnormalities and DNA single-strand breaks). At 11 days of the exposure phase, tadpoles recovered their basal levels of frequency of MNs, other nuclear abnormalities, and comets. However, loss of keratodonts, observed at the end of the exposure period, was present up to 21 days thereafter. Finally, axial abnormalities and delay in development stage were observed only during the postexposure phase in IMZT-exposed tadpoles at 18 and 25 days, respectively and were observed until the end of the experiment. This is the first evidence of use the comet assay as cytogenetic biomarker of genotoxicity in evaluating the recovery capabilities of amphibians in general and also those of <i>B. pulchella</i> after exposure to IMZT.Facultad de Ciencias Naturales y MuseoFacultad de Ciencias ExactasCentro de Investigaciones del Medioambiente2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1-10http://sedici.unlp.edu.ar/handle/10915/109182enginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0147651317306814info:eu-repo/semantics/altIdentifier/issn/0147-6513info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2017.10.009info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:24:42Zoai:sedici.unlp.edu.ar:10915/109182Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:24:43.234SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Are the damaging effects induced by the imazethapyr formulation Pivot® H in <i>Boana pulchella</i> (Anura) reversible upon ceasing exposure? |
title |
Are the damaging effects induced by the imazethapyr formulation Pivot® H in <i>Boana pulchella</i> (Anura) reversible upon ceasing exposure? |
spellingShingle |
Are the damaging effects induced by the imazethapyr formulation Pivot® H in <i>Boana pulchella</i> (Anura) reversible upon ceasing exposure? Pérez Iglesias, Juan Manuel Ciencias Naturales Ciencias Exactas imazethapyr Pivot® H recovery capabilities comet assay micronuclei morphological abnormalities |
title_short |
Are the damaging effects induced by the imazethapyr formulation Pivot® H in <i>Boana pulchella</i> (Anura) reversible upon ceasing exposure? |
title_full |
Are the damaging effects induced by the imazethapyr formulation Pivot® H in <i>Boana pulchella</i> (Anura) reversible upon ceasing exposure? |
title_fullStr |
Are the damaging effects induced by the imazethapyr formulation Pivot® H in <i>Boana pulchella</i> (Anura) reversible upon ceasing exposure? |
title_full_unstemmed |
Are the damaging effects induced by the imazethapyr formulation Pivot® H in <i>Boana pulchella</i> (Anura) reversible upon ceasing exposure? |
title_sort |
Are the damaging effects induced by the imazethapyr formulation Pivot® H in <i>Boana pulchella</i> (Anura) reversible upon ceasing exposure? |
dc.creator.none.fl_str_mv |
Pérez Iglesias, Juan Manuel Natale, Guillermo Sebastián Soloneski, Sonia María Elsa Larramendy, Marcelo Luis |
author |
Pérez Iglesias, Juan Manuel |
author_facet |
Pérez Iglesias, Juan Manuel Natale, Guillermo Sebastián Soloneski, Sonia María Elsa Larramendy, Marcelo Luis |
author_role |
author |
author2 |
Natale, Guillermo Sebastián Soloneski, Sonia María Elsa Larramendy, Marcelo Luis |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Ciencias Naturales Ciencias Exactas imazethapyr Pivot® H recovery capabilities comet assay micronuclei morphological abnormalities |
topic |
Ciencias Naturales Ciencias Exactas imazethapyr Pivot® H recovery capabilities comet assay micronuclei morphological abnormalities |
dc.description.none.fl_txt_mv |
In the present study, the damage recovery capabilities of <i>Boana pulchella</i> tadpoles after acute exposure (96 h) to 0.39 mg/L concentration of the imazethapyr (IMZT)-based herbicide formulation Pivot® H (25% IMZT LC<sub>50</sub> value) were assessed during a period of 7 to −21 days. To appraise the recovery capabilities, frequency of micronuclei (MNs), other nuclear abnormalities and DNA single-strand breaks evaluated by single cell gel electrophoresis assay on circulating blood cells were employed as endpoints for genotoxicity. Growth, development, body mass, and morphological abnormalities were also employed as individual endpoints in the recovery assay. Results demonstrated that IMZT induced sublethal effects at both the individual (i.e., loss of keratodonts) and cytogenetic levels (e.g., increase of MN frequency, other nuclear abnormalities and DNA single-strand breaks). At 11 days of the exposure phase, tadpoles recovered their basal levels of frequency of MNs, other nuclear abnormalities, and comets. However, loss of keratodonts, observed at the end of the exposure period, was present up to 21 days thereafter. Finally, axial abnormalities and delay in development stage were observed only during the postexposure phase in IMZT-exposed tadpoles at 18 and 25 days, respectively and were observed until the end of the experiment. This is the first evidence of use the comet assay as cytogenetic biomarker of genotoxicity in evaluating the recovery capabilities of amphibians in general and also those of <i>B. pulchella</i> after exposure to IMZT. Facultad de Ciencias Naturales y Museo Facultad de Ciencias Exactas Centro de Investigaciones del Medioambiente |
description |
In the present study, the damage recovery capabilities of <i>Boana pulchella</i> tadpoles after acute exposure (96 h) to 0.39 mg/L concentration of the imazethapyr (IMZT)-based herbicide formulation Pivot® H (25% IMZT LC<sub>50</sub> value) were assessed during a period of 7 to −21 days. To appraise the recovery capabilities, frequency of micronuclei (MNs), other nuclear abnormalities and DNA single-strand breaks evaluated by single cell gel electrophoresis assay on circulating blood cells were employed as endpoints for genotoxicity. Growth, development, body mass, and morphological abnormalities were also employed as individual endpoints in the recovery assay. Results demonstrated that IMZT induced sublethal effects at both the individual (i.e., loss of keratodonts) and cytogenetic levels (e.g., increase of MN frequency, other nuclear abnormalities and DNA single-strand breaks). At 11 days of the exposure phase, tadpoles recovered their basal levels of frequency of MNs, other nuclear abnormalities, and comets. However, loss of keratodonts, observed at the end of the exposure period, was present up to 21 days thereafter. Finally, axial abnormalities and delay in development stage were observed only during the postexposure phase in IMZT-exposed tadpoles at 18 and 25 days, respectively and were observed until the end of the experiment. This is the first evidence of use the comet assay as cytogenetic biomarker of genotoxicity in evaluating the recovery capabilities of amphibians in general and also those of <i>B. pulchella</i> after exposure to IMZT. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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http://sedici.unlp.edu.ar/handle/10915/109182 |
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http://sedici.unlp.edu.ar/handle/10915/109182 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
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dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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