Novel biopolymer matrices for microencapsulation of phages: enhanced protection against acidity and protease activity

Autores
Dini, Cecilia; Islan, Germán Abel; Urraza, Patricio José de; Castro, Guillermo Raúl
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Phage therapy by oral administration requires enhanced resistance of phages to the harsh gastric conditions. The aim of this work is the microencapsulation of phages in natural biopolymeric matrices as a protective barrier against the gastric environment. Alginate and pectin are used as base polymers. Further emulsification with oleic acid or coating with a different biopolymer is also studied. Emulsified pectin shows the maximum encapsulation efficiency and the highest protection against acidity, leaving more than 10³ active phages after 30 min exposure at pH¼1.6, and protects phage from pepsin activity (4.2mgmL⁻¹). Non-encapsulated phages are fully inactivated at pH¼1.6 or with pepsin (0.5mgmL⁻¹) after 10 min.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Biología
Biocontrol
Biological applications of polymers
Biopolymers
Microencapsulation
Phages
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/153287
Acceder
id SEDICI_8a975ff6c7325e3f9bb947604e300693
oai_identifier_str oai:sedici.unlp.edu.ar:10915/153287
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Novel biopolymer matrices for microencapsulation of phages: enhanced protection against acidity and protease activityDini, CeciliaIslan, Germán AbelUrraza, Patricio José deCastro, Guillermo RaúlBiologíaBiocontrolBiological applications of polymersBiopolymersMicroencapsulationPhagesPhage therapy by oral administration requires enhanced resistance of phages to the harsh gastric conditions. The aim of this work is the microencapsulation of phages in natural biopolymeric matrices as a protective barrier against the gastric environment. Alginate and pectin are used as base polymers. Further emulsification with oleic acid or coating with a different biopolymer is also studied. Emulsified pectin shows the maximum encapsulation efficiency and the highest protection against acidity, leaving more than 10³ active phages after 30 min exposure at pH¼1.6, and protects phage from pepsin activity (4.2mgmL⁻¹). Non-encapsulated phages are fully inactivated at pH¼1.6 or with pepsin (0.5mgmL⁻¹) after 10 min.Centro de Investigación y Desarrollo en Fermentaciones Industriales2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1200–1208http://sedici.unlp.edu.ar/handle/10915/153287enginfo:eu-repo/semantics/altIdentifier/issn/1616-5195info:eu-repo/semantics/altIdentifier/doi/10.1002/mabi.201200109info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:11:38Zoai:sedici.unlp.edu.ar:10915/153287Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:11:39.217SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Novel biopolymer matrices for microencapsulation of phages: enhanced protection against acidity and protease activity
title Novel biopolymer matrices for microencapsulation of phages: enhanced protection against acidity and protease activity
spellingShingle Novel biopolymer matrices for microencapsulation of phages: enhanced protection against acidity and protease activity
Dini, Cecilia
Biología
Biocontrol
Biological applications of polymers
Biopolymers
Microencapsulation
Phages
title_short Novel biopolymer matrices for microencapsulation of phages: enhanced protection against acidity and protease activity
title_full Novel biopolymer matrices for microencapsulation of phages: enhanced protection against acidity and protease activity
title_fullStr Novel biopolymer matrices for microencapsulation of phages: enhanced protection against acidity and protease activity
title_full_unstemmed Novel biopolymer matrices for microencapsulation of phages: enhanced protection against acidity and protease activity
title_sort Novel biopolymer matrices for microencapsulation of phages: enhanced protection against acidity and protease activity
dc.creator.none.fl_str_mv Dini, Cecilia
Islan, Germán Abel
Urraza, Patricio José de
Castro, Guillermo Raúl
author Dini, Cecilia
author_facet Dini, Cecilia
Islan, Germán Abel
Urraza, Patricio José de
Castro, Guillermo Raúl
author_role author
author2 Islan, Germán Abel
Urraza, Patricio José de
Castro, Guillermo Raúl
author2_role author
author
author
dc.subject.none.fl_str_mv Biología
Biocontrol
Biological applications of polymers
Biopolymers
Microencapsulation
Phages
topic Biología
Biocontrol
Biological applications of polymers
Biopolymers
Microencapsulation
Phages
dc.description.none.fl_txt_mv Phage therapy by oral administration requires enhanced resistance of phages to the harsh gastric conditions. The aim of this work is the microencapsulation of phages in natural biopolymeric matrices as a protective barrier against the gastric environment. Alginate and pectin are used as base polymers. Further emulsification with oleic acid or coating with a different biopolymer is also studied. Emulsified pectin shows the maximum encapsulation efficiency and the highest protection against acidity, leaving more than 10³ active phages after 30 min exposure at pH¼1.6, and protects phage from pepsin activity (4.2mgmL⁻¹). Non-encapsulated phages are fully inactivated at pH¼1.6 or with pepsin (0.5mgmL⁻¹) after 10 min.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description Phage therapy by oral administration requires enhanced resistance of phages to the harsh gastric conditions. The aim of this work is the microencapsulation of phages in natural biopolymeric matrices as a protective barrier against the gastric environment. Alginate and pectin are used as base polymers. Further emulsification with oleic acid or coating with a different biopolymer is also studied. Emulsified pectin shows the maximum encapsulation efficiency and the highest protection against acidity, leaving more than 10³ active phages after 30 min exposure at pH¼1.6, and protects phage from pepsin activity (4.2mgmL⁻¹). Non-encapsulated phages are fully inactivated at pH¼1.6 or with pepsin (0.5mgmL⁻¹) after 10 min.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/153287
url http://sedici.unlp.edu.ar/handle/10915/153287
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1616-5195
info:eu-repo/semantics/altIdentifier/doi/10.1002/mabi.201200109
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
1200–1208
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1842260620497387520
score 13.13397

Publicaciones similares