Restorative effect of insulin-like growth factor-I gene therapy in the hypothalamus of senile rats with dopaminergic dysfunction
- Autores
- Hereñú, Claudia Beatriz; Cristina, Silvia Carolina; Rimoldi, Omar Jorge; Becú de Villalobos, Damasia; Cambiaggi, Vanina Laura; Portiansky, Enrique Leo; Goya, Rodolfo Gustavo
- Año de publicación
- 2007
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Insulin-like growth factor-I (IGF-I) is emerging as a powerful neuroprotective molecule that is strongly induced in the central nervous system after different insults. We constructed a recombinant adenoviral vector (RAd-IGFI) harboring the gene for rat IGF-I and used it to implement IGF-I gene therapy in the hypothalamus of senile female rats, which display hypothalamic dopaminergic (DA) neurodegeneration and as a consequence, chronic hyperprolactinemia. Restorative IGF-I gene therapy was implemented in young (5 months) and senile (28 months) female rats, which received a single intrahypothalamic injection of 3 × 109 plaque-forming units of RAd-βgal (a control adenoviral vector expressing β-galactosidase) or RAd-IGFI and were killed 17 days post-injection. In the young animals, neither vector modified serum prolactin levels, but in the RAd-IGFI-injected senile rats a nearly full reversion of their hyperprolactinemic status was recorded. Morphometric analysis revealed a significant increase in the total number of tyrosine hydroxylase-positive cells in the hypothalamus of experimental as compared with control senile animals (5874±486 and 3390±498, respectively). Our results indicate that IGF-I gene therapy in senile female rats is highly effective for restoring their hypothalamic DA dysfunction and thus reversing their chronic hyperprolactinemia.
Instituto de Investigaciones Bioquímicas de La Plata
Facultad de Ciencias Médicas
Facultad de Ciencias Veterinarias
Facultad de Ciencias Exactas - Materia
-
Ciencias Médicas
Ciencias Veterinarias
aging
DA neurodegeneration
TIDA neurons
hyperprolactinemia
IGF-I - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/82997
Ver los metadatos del registro completo
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Restorative effect of insulin-like growth factor-I gene therapy in the hypothalamus of senile rats with dopaminergic dysfunctionHereñú, Claudia BeatrizCristina, Silvia CarolinaRimoldi, Omar JorgeBecú de Villalobos, DamasiaCambiaggi, Vanina LauraPortiansky, Enrique LeoGoya, Rodolfo GustavoCiencias MédicasCiencias VeterinariasagingDA neurodegenerationTIDA neuronshyperprolactinemiaIGF-IInsulin-like growth factor-I (IGF-I) is emerging as a powerful neuroprotective molecule that is strongly induced in the central nervous system after different insults. We constructed a recombinant adenoviral vector (RAd-IGFI) harboring the gene for rat IGF-I and used it to implement IGF-I gene therapy in the hypothalamus of senile female rats, which display hypothalamic dopaminergic (DA) neurodegeneration and as a consequence, chronic hyperprolactinemia. Restorative IGF-I gene therapy was implemented in young (5 months) and senile (28 months) female rats, which received a single intrahypothalamic injection of 3 × 10<SUP>9</SUP> plaque-forming units of RAd-βgal (a control adenoviral vector expressing β-galactosidase) or RAd-IGFI and were killed 17 days post-injection. In the young animals, neither vector modified serum prolactin levels, but in the RAd-IGFI-injected senile rats a nearly full reversion of their hyperprolactinemic status was recorded. Morphometric analysis revealed a significant increase in the total number of tyrosine hydroxylase-positive cells in the hypothalamus of experimental as compared with control senile animals (5874±486 and 3390±498, respectively). Our results indicate that IGF-I gene therapy in senile female rats is highly effective for restoring their hypothalamic DA dysfunction and thus reversing their chronic hyperprolactinemia.Instituto de Investigaciones Bioquímicas de La PlataFacultad de Ciencias MédicasFacultad de Ciencias VeterinariasFacultad de Ciencias Exactas2007info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf237-245http://sedici.unlp.edu.ar/handle/10915/82997enginfo:eu-repo/semantics/altIdentifier/issn/0969-7128info:eu-repo/semantics/altIdentifier/doi/10.1038/sj.gt.3302870info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:15:45Zoai:sedici.unlp.edu.ar:10915/82997Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:15:45.878SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
Restorative effect of insulin-like growth factor-I gene therapy in the hypothalamus of senile rats with dopaminergic dysfunction |
| title |
Restorative effect of insulin-like growth factor-I gene therapy in the hypothalamus of senile rats with dopaminergic dysfunction |
| spellingShingle |
Restorative effect of insulin-like growth factor-I gene therapy in the hypothalamus of senile rats with dopaminergic dysfunction Hereñú, Claudia Beatriz Ciencias Médicas Ciencias Veterinarias aging DA neurodegeneration TIDA neurons hyperprolactinemia IGF-I |
| title_short |
Restorative effect of insulin-like growth factor-I gene therapy in the hypothalamus of senile rats with dopaminergic dysfunction |
| title_full |
Restorative effect of insulin-like growth factor-I gene therapy in the hypothalamus of senile rats with dopaminergic dysfunction |
| title_fullStr |
Restorative effect of insulin-like growth factor-I gene therapy in the hypothalamus of senile rats with dopaminergic dysfunction |
| title_full_unstemmed |
Restorative effect of insulin-like growth factor-I gene therapy in the hypothalamus of senile rats with dopaminergic dysfunction |
| title_sort |
Restorative effect of insulin-like growth factor-I gene therapy in the hypothalamus of senile rats with dopaminergic dysfunction |
| dc.creator.none.fl_str_mv |
Hereñú, Claudia Beatriz Cristina, Silvia Carolina Rimoldi, Omar Jorge Becú de Villalobos, Damasia Cambiaggi, Vanina Laura Portiansky, Enrique Leo Goya, Rodolfo Gustavo |
| author |
Hereñú, Claudia Beatriz |
| author_facet |
Hereñú, Claudia Beatriz Cristina, Silvia Carolina Rimoldi, Omar Jorge Becú de Villalobos, Damasia Cambiaggi, Vanina Laura Portiansky, Enrique Leo Goya, Rodolfo Gustavo |
| author_role |
author |
| author2 |
Cristina, Silvia Carolina Rimoldi, Omar Jorge Becú de Villalobos, Damasia Cambiaggi, Vanina Laura Portiansky, Enrique Leo Goya, Rodolfo Gustavo |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
Ciencias Médicas Ciencias Veterinarias aging DA neurodegeneration TIDA neurons hyperprolactinemia IGF-I |
| topic |
Ciencias Médicas Ciencias Veterinarias aging DA neurodegeneration TIDA neurons hyperprolactinemia IGF-I |
| dc.description.none.fl_txt_mv |
Insulin-like growth factor-I (IGF-I) is emerging as a powerful neuroprotective molecule that is strongly induced in the central nervous system after different insults. We constructed a recombinant adenoviral vector (RAd-IGFI) harboring the gene for rat IGF-I and used it to implement IGF-I gene therapy in the hypothalamus of senile female rats, which display hypothalamic dopaminergic (DA) neurodegeneration and as a consequence, chronic hyperprolactinemia. Restorative IGF-I gene therapy was implemented in young (5 months) and senile (28 months) female rats, which received a single intrahypothalamic injection of 3 × 10<SUP>9</SUP> plaque-forming units of RAd-βgal (a control adenoviral vector expressing β-galactosidase) or RAd-IGFI and were killed 17 days post-injection. In the young animals, neither vector modified serum prolactin levels, but in the RAd-IGFI-injected senile rats a nearly full reversion of their hyperprolactinemic status was recorded. Morphometric analysis revealed a significant increase in the total number of tyrosine hydroxylase-positive cells in the hypothalamus of experimental as compared with control senile animals (5874±486 and 3390±498, respectively). Our results indicate that IGF-I gene therapy in senile female rats is highly effective for restoring their hypothalamic DA dysfunction and thus reversing their chronic hyperprolactinemia. Instituto de Investigaciones Bioquímicas de La Plata Facultad de Ciencias Médicas Facultad de Ciencias Veterinarias Facultad de Ciencias Exactas |
| description |
Insulin-like growth factor-I (IGF-I) is emerging as a powerful neuroprotective molecule that is strongly induced in the central nervous system after different insults. We constructed a recombinant adenoviral vector (RAd-IGFI) harboring the gene for rat IGF-I and used it to implement IGF-I gene therapy in the hypothalamus of senile female rats, which display hypothalamic dopaminergic (DA) neurodegeneration and as a consequence, chronic hyperprolactinemia. Restorative IGF-I gene therapy was implemented in young (5 months) and senile (28 months) female rats, which received a single intrahypothalamic injection of 3 × 10<SUP>9</SUP> plaque-forming units of RAd-βgal (a control adenoviral vector expressing β-galactosidase) or RAd-IGFI and were killed 17 days post-injection. In the young animals, neither vector modified serum prolactin levels, but in the RAd-IGFI-injected senile rats a nearly full reversion of their hyperprolactinemic status was recorded. Morphometric analysis revealed a significant increase in the total number of tyrosine hydroxylase-positive cells in the hypothalamus of experimental as compared with control senile animals (5874±486 and 3390±498, respectively). Our results indicate that IGF-I gene therapy in senile female rats is highly effective for restoring their hypothalamic DA dysfunction and thus reversing their chronic hyperprolactinemia. |
| publishDate |
2007 |
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2007 |
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eng |
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