Detection of equine herpesvirus 1 genome 1B in Argentina
- Autores
- Martínez, J. P.; Martín Ocampos, Giselle Paula; Fernandez, L. C.; Fuentealba, Nadia Analía; Cid de La Paz, Viviana; Barrandeguy, M.; Galosi, Cecilia Mónica
- Año de publicación
- 2006
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- To determine the genomic variation of equine herpesviruses (EHVs) isolated in Argentina between 1979 and the first half of 2004, DNA sequences from all 69 strains isolated were analysed. Sixty strains were recovered from aborted fetuses, one from leucocyte-rich plasma from a horse with respiratory signs and eight from cases of neonatal disease. The DNA was extracted from rabbit kidney epithelial (RK13) cells infected with each strain and digested with three restriction endonucleases (BamHI, Bg/II and KpnI). Two strains could be differentiated using BamHI restriction and were assigned to the EHV-1 1B prototype group. Only one of these two strains was typed EHV-1 1B with Bg/II. DNA digestion with KpnI was ineffective. The results obtained in this study demonstrate that the EHV-1 1B genome has been present in Argentina since at least 1996. The finding of two strains with this electropherotype suggests that there is genomic heterogeneity among Argentinian isolates.
Facultad de Ciencias Veterinarias - Materia
-
Ciencias Veterinarias
1B genome
Argentina
Equine herpesvirus 1
First detection
Restriction endonuclease analysis - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/83202
Ver los metadatos del registro completo
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Detection of equine herpesvirus 1 genome 1B in ArgentinaMartínez, J. P.Martín Ocampos, Giselle PaulaFernandez, L. C.Fuentealba, Nadia AnalíaCid de La Paz, VivianaBarrandeguy, M.Galosi, Cecilia MónicaCiencias Veterinarias1B genomeArgentinaEquine herpesvirus 1First detectionRestriction endonuclease analysisTo determine the genomic variation of equine herpesviruses (EHVs) isolated in Argentina between 1979 and the first half of 2004, DNA sequences from all 69 strains isolated were analysed. Sixty strains were recovered from aborted fetuses, one from leucocyte-rich plasma from a horse with respiratory signs and eight from cases of neonatal disease. The DNA was extracted from rabbit kidney epithelial (RK13) cells infected with each strain and digested with three restriction endonucleases (BamHI, Bg/II and KpnI). Two strains could be differentiated using BamHI restriction and were assigned to the EHV-1 1B prototype group. Only one of these two strains was typed EHV-1 1B with Bg/II. DNA digestion with KpnI was ineffective. The results obtained in this study demonstrate that the EHV-1 1B genome has been present in Argentina since at least 1996. The finding of two strains with this electropherotype suggests that there is genomic heterogeneity among Argentinian isolates.Facultad de Ciencias Veterinarias2006info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1075-1079http://sedici.unlp.edu.ar/handle/10915/83202enginfo:eu-repo/semantics/altIdentifier/issn/0253-1933info:eu-repo/semantics/altIdentifier/doi/10.20506/rst.25.3.1711info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:15:46Zoai:sedici.unlp.edu.ar:10915/83202Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:15:46.846SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
Detection of equine herpesvirus 1 genome 1B in Argentina |
| title |
Detection of equine herpesvirus 1 genome 1B in Argentina |
| spellingShingle |
Detection of equine herpesvirus 1 genome 1B in Argentina Martínez, J. P. Ciencias Veterinarias 1B genome Argentina Equine herpesvirus 1 First detection Restriction endonuclease analysis |
| title_short |
Detection of equine herpesvirus 1 genome 1B in Argentina |
| title_full |
Detection of equine herpesvirus 1 genome 1B in Argentina |
| title_fullStr |
Detection of equine herpesvirus 1 genome 1B in Argentina |
| title_full_unstemmed |
Detection of equine herpesvirus 1 genome 1B in Argentina |
| title_sort |
Detection of equine herpesvirus 1 genome 1B in Argentina |
| dc.creator.none.fl_str_mv |
Martínez, J. P. Martín Ocampos, Giselle Paula Fernandez, L. C. Fuentealba, Nadia Analía Cid de La Paz, Viviana Barrandeguy, M. Galosi, Cecilia Mónica |
| author |
Martínez, J. P. |
| author_facet |
Martínez, J. P. Martín Ocampos, Giselle Paula Fernandez, L. C. Fuentealba, Nadia Analía Cid de La Paz, Viviana Barrandeguy, M. Galosi, Cecilia Mónica |
| author_role |
author |
| author2 |
Martín Ocampos, Giselle Paula Fernandez, L. C. Fuentealba, Nadia Analía Cid de La Paz, Viviana Barrandeguy, M. Galosi, Cecilia Mónica |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
Ciencias Veterinarias 1B genome Argentina Equine herpesvirus 1 First detection Restriction endonuclease analysis |
| topic |
Ciencias Veterinarias 1B genome Argentina Equine herpesvirus 1 First detection Restriction endonuclease analysis |
| dc.description.none.fl_txt_mv |
To determine the genomic variation of equine herpesviruses (EHVs) isolated in Argentina between 1979 and the first half of 2004, DNA sequences from all 69 strains isolated were analysed. Sixty strains were recovered from aborted fetuses, one from leucocyte-rich plasma from a horse with respiratory signs and eight from cases of neonatal disease. The DNA was extracted from rabbit kidney epithelial (RK13) cells infected with each strain and digested with three restriction endonucleases (BamHI, Bg/II and KpnI). Two strains could be differentiated using BamHI restriction and were assigned to the EHV-1 1B prototype group. Only one of these two strains was typed EHV-1 1B with Bg/II. DNA digestion with KpnI was ineffective. The results obtained in this study demonstrate that the EHV-1 1B genome has been present in Argentina since at least 1996. The finding of two strains with this electropherotype suggests that there is genomic heterogeneity among Argentinian isolates. Facultad de Ciencias Veterinarias |
| description |
To determine the genomic variation of equine herpesviruses (EHVs) isolated in Argentina between 1979 and the first half of 2004, DNA sequences from all 69 strains isolated were analysed. Sixty strains were recovered from aborted fetuses, one from leucocyte-rich plasma from a horse with respiratory signs and eight from cases of neonatal disease. The DNA was extracted from rabbit kidney epithelial (RK13) cells infected with each strain and digested with three restriction endonucleases (BamHI, Bg/II and KpnI). Two strains could be differentiated using BamHI restriction and were assigned to the EHV-1 1B prototype group. Only one of these two strains was typed EHV-1 1B with Bg/II. DNA digestion with KpnI was ineffective. The results obtained in this study demonstrate that the EHV-1 1B genome has been present in Argentina since at least 1996. The finding of two strains with this electropherotype suggests that there is genomic heterogeneity among Argentinian isolates. |
| publishDate |
2006 |
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2006 |
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eng |
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