Detection of equine herpesvirus 1 genome 1B in Argentina

Autores
Martínez, J. P.; Martín Ocampos, Giselle Paula; Fernandez, L. C.; Fuentealba, Nadia Analía; Cid de La Paz, Viviana; Barrandeguy, M.; Galosi, Cecilia Mónica
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To determine the genomic variation of equine herpesviruses (EHVs) isolated in Argentina between 1979 and the first half of 2004, DNA sequences from all 69 strains isolated were analysed. Sixty strains were recovered from aborted fetuses, one from leucocyte-rich plasma from a horse with respiratory signs and eight from cases of neonatal disease. The DNA was extracted from rabbit kidney epithelial (RK13) cells infected with each strain and digested with three restriction endonucleases (BamHI, Bg/II and KpnI). Two strains could be differentiated using BamHI restriction and were assigned to the EHV-1 1B prototype group. Only one of these two strains was typed EHV-1 1B with Bg/II. DNA digestion with KpnI was ineffective. The results obtained in this study demonstrate that the EHV-1 1B genome has been present in Argentina since at least 1996. The finding of two strains with this electropherotype suggests that there is genomic heterogeneity among Argentinian isolates.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
1B genome
Argentina
Equine herpesvirus 1
First detection
Restriction endonuclease analysis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/83202
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/83202
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Detection of equine herpesvirus 1 genome 1B in ArgentinaMartínez, J. P.Martín Ocampos, Giselle PaulaFernandez, L. C.Fuentealba, Nadia AnalíaCid de La Paz, VivianaBarrandeguy, M.Galosi, Cecilia MónicaCiencias Veterinarias1B genomeArgentinaEquine herpesvirus 1First detectionRestriction endonuclease analysisTo determine the genomic variation of equine herpesviruses (EHVs) isolated in Argentina between 1979 and the first half of 2004, DNA sequences from all 69 strains isolated were analysed. Sixty strains were recovered from aborted fetuses, one from leucocyte-rich plasma from a horse with respiratory signs and eight from cases of neonatal disease. The DNA was extracted from rabbit kidney epithelial (RK13) cells infected with each strain and digested with three restriction endonucleases (BamHI, Bg/II and KpnI). Two strains could be differentiated using BamHI restriction and were assigned to the EHV-1 1B prototype group. Only one of these two strains was typed EHV-1 1B with Bg/II. DNA digestion with KpnI was ineffective. The results obtained in this study demonstrate that the EHV-1 1B genome has been present in Argentina since at least 1996. The finding of two strains with this electropherotype suggests that there is genomic heterogeneity among Argentinian isolates.Facultad de Ciencias Veterinarias2006info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1075-1079http://sedici.unlp.edu.ar/handle/10915/83202enginfo:eu-repo/semantics/altIdentifier/issn/0253-1933info:eu-repo/semantics/altIdentifier/doi/10.20506/rst.25.3.1711info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:48:01Zoai:sedici.unlp.edu.ar:10915/83202Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:48:02.091SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Detection of equine herpesvirus 1 genome 1B in Argentina
title Detection of equine herpesvirus 1 genome 1B in Argentina
spellingShingle Detection of equine herpesvirus 1 genome 1B in Argentina
Martínez, J. P.
Ciencias Veterinarias
1B genome
Argentina
Equine herpesvirus 1
First detection
Restriction endonuclease analysis
title_short Detection of equine herpesvirus 1 genome 1B in Argentina
title_full Detection of equine herpesvirus 1 genome 1B in Argentina
title_fullStr Detection of equine herpesvirus 1 genome 1B in Argentina
title_full_unstemmed Detection of equine herpesvirus 1 genome 1B in Argentina
title_sort Detection of equine herpesvirus 1 genome 1B in Argentina
dc.creator.none.fl_str_mv Martínez, J. P.
Martín Ocampos, Giselle Paula
Fernandez, L. C.
Fuentealba, Nadia Analía
Cid de La Paz, Viviana
Barrandeguy, M.
Galosi, Cecilia Mónica
author Martínez, J. P.
author_facet Martínez, J. P.
Martín Ocampos, Giselle Paula
Fernandez, L. C.
Fuentealba, Nadia Analía
Cid de La Paz, Viviana
Barrandeguy, M.
Galosi, Cecilia Mónica
author_role author
author2 Martín Ocampos, Giselle Paula
Fernandez, L. C.
Fuentealba, Nadia Analía
Cid de La Paz, Viviana
Barrandeguy, M.
Galosi, Cecilia Mónica
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
1B genome
Argentina
Equine herpesvirus 1
First detection
Restriction endonuclease analysis
topic Ciencias Veterinarias
1B genome
Argentina
Equine herpesvirus 1
First detection
Restriction endonuclease analysis
dc.description.none.fl_txt_mv To determine the genomic variation of equine herpesviruses (EHVs) isolated in Argentina between 1979 and the first half of 2004, DNA sequences from all 69 strains isolated were analysed. Sixty strains were recovered from aborted fetuses, one from leucocyte-rich plasma from a horse with respiratory signs and eight from cases of neonatal disease. The DNA was extracted from rabbit kidney epithelial (RK13) cells infected with each strain and digested with three restriction endonucleases (BamHI, Bg/II and KpnI). Two strains could be differentiated using BamHI restriction and were assigned to the EHV-1 1B prototype group. Only one of these two strains was typed EHV-1 1B with Bg/II. DNA digestion with KpnI was ineffective. The results obtained in this study demonstrate that the EHV-1 1B genome has been present in Argentina since at least 1996. The finding of two strains with this electropherotype suggests that there is genomic heterogeneity among Argentinian isolates.
Facultad de Ciencias Veterinarias
description To determine the genomic variation of equine herpesviruses (EHVs) isolated in Argentina between 1979 and the first half of 2004, DNA sequences from all 69 strains isolated were analysed. Sixty strains were recovered from aborted fetuses, one from leucocyte-rich plasma from a horse with respiratory signs and eight from cases of neonatal disease. The DNA was extracted from rabbit kidney epithelial (RK13) cells infected with each strain and digested with three restriction endonucleases (BamHI, Bg/II and KpnI). Two strains could be differentiated using BamHI restriction and were assigned to the EHV-1 1B prototype group. Only one of these two strains was typed EHV-1 1B with Bg/II. DNA digestion with KpnI was ineffective. The results obtained in this study demonstrate that the EHV-1 1B genome has been present in Argentina since at least 1996. The finding of two strains with this electropherotype suggests that there is genomic heterogeneity among Argentinian isolates.
publishDate 2006
dc.date.none.fl_str_mv 2006
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/83202
url http://sedici.unlp.edu.ar/handle/10915/83202
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0253-1933
info:eu-repo/semantics/altIdentifier/doi/10.20506/rst.25.3.1711
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
1075-1079
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.13397

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