Sarcocystosis in wild red deer (<i>Cervus elaphus</i>) in Patagonia, Argentina

Autores
Chang Reissig, Elizabeth; Moré, Gastón Andrés; Massone, Adriana Raquel; Uzal, Francisco A.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sarcocystis spp. are protozoan parasites with a heteroxenous life cycle, which produce cysts in the muscle of herbivorous animals. In these animal species, sarcocystosis is frequently asymptomatic, although it may occur with high prevalence. Seven Sarcocystis spp. have been described in red deer (Cervus elephus). The aim of this study was to determine the prevalence of sarcocystosis, and to perform the morphological and molecular characterization of Sarcocystis spp. found in wild red deer of the Nahuel Huapi National Park (NHNP), Patagonia, Argentina. Full necropsies of 62 red deer killed by hunters in the NHNP and neighboring areas were performed. Samples of heart and skeletal muscle were examined histologically and selected samples were also examined by transmission electron microscopy (TEM), PCR and sequencing. Sarcocystis spp. thin walled cysts were detected in 62 % (38/62) of heart, and in 22 % (3/14) of skeletal muscle samples examined histologically. TEM revealed a smooth and thin cyst wall (≤1 μm), with scarce and separated ribbon-like protrusions. A total of three partial and one full 18S ribosomal RNA (rRNA) gene sequences were obtained, and showed the highest identity (≥99 %) with Sarcocystis taeniata, a species described in moose (Alces alces). The morphological and molecular results indicate that red deer in Argentina are frequently infected with S. taeniata, a species for which the definitive host is unknown. The present results also confirm that Sarcocystis spp. using cervids as intermediate host are not host-specific. Further studies are needed to improve the epidemiological knowledge of Sarcocystosis in red deer.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
Sarcocystis taeniata
Red deer
Argentinean nonnative ungulate
TEM
Sequencing
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/139537
Acceder
id SEDICI_7f496c2709a61627e7a69b1e3920d11b
oai_identifier_str oai:sedici.unlp.edu.ar:10915/139537
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Sarcocystosis in wild red deer (<i>Cervus elaphus</i>) in Patagonia, ArgentinaChang Reissig, ElizabethMoré, Gastón AndrésMassone, Adriana RaquelUzal, Francisco A.Ciencias VeterinariasSarcocystis taeniataRed deerArgentinean nonnative ungulateTEMSequencingSarcocystis spp. are protozoan parasites with a heteroxenous life cycle, which produce cysts in the muscle of herbivorous animals. In these animal species, sarcocystosis is frequently asymptomatic, although it may occur with high prevalence. Seven Sarcocystis spp. have been described in red deer (Cervus elephus). The aim of this study was to determine the prevalence of sarcocystosis, and to perform the morphological and molecular characterization of Sarcocystis spp. found in wild red deer of the Nahuel Huapi National Park (NHNP), Patagonia, Argentina. Full necropsies of 62 red deer killed by hunters in the NHNP and neighboring areas were performed. Samples of heart and skeletal muscle were examined histologically and selected samples were also examined by transmission electron microscopy (TEM), PCR and sequencing. Sarcocystis spp. thin walled cysts were detected in 62 % (38/62) of heart, and in 22 % (3/14) of skeletal muscle samples examined histologically. TEM revealed a smooth and thin cyst wall (≤1 μm), with scarce and separated ribbon-like protrusions. A total of three partial and one full 18S ribosomal RNA (rRNA) gene sequences were obtained, and showed the highest identity (≥99 %) with Sarcocystis taeniata, a species described in moose (Alces alces). The morphological and molecular results indicate that red deer in Argentina are frequently infected with S. taeniata, a species for which the definitive host is unknown. The present results also confirm that Sarcocystis spp. using cervids as intermediate host are not host-specific. Further studies are needed to improve the epidemiological knowledge of Sarcocystosis in red deer.Facultad de Ciencias Veterinarias2016-01-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1773-1778http://sedici.unlp.edu.ar/handle/10915/139537enginfo:eu-repo/semantics/altIdentifier/issn/1432-1955info:eu-repo/semantics/altIdentifier/issn/0932-0113info:eu-repo/semantics/altIdentifier/doi/10.1007/s00436-016-4915-7info:eu-repo/semantics/altIdentifier/pmid/26779923info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:31Zoai:sedici.unlp.edu.ar:10915/139537Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:32.184SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Sarcocystosis in wild red deer (<i>Cervus elaphus</i>) in Patagonia, Argentina
title Sarcocystosis in wild red deer (<i>Cervus elaphus</i>) in Patagonia, Argentina
spellingShingle Sarcocystosis in wild red deer (<i>Cervus elaphus</i>) in Patagonia, Argentina
Chang Reissig, Elizabeth
Ciencias Veterinarias
Sarcocystis taeniata
Red deer
Argentinean nonnative ungulate
TEM
Sequencing
title_short Sarcocystosis in wild red deer (<i>Cervus elaphus</i>) in Patagonia, Argentina
title_full Sarcocystosis in wild red deer (<i>Cervus elaphus</i>) in Patagonia, Argentina
title_fullStr Sarcocystosis in wild red deer (<i>Cervus elaphus</i>) in Patagonia, Argentina
title_full_unstemmed Sarcocystosis in wild red deer (<i>Cervus elaphus</i>) in Patagonia, Argentina
title_sort Sarcocystosis in wild red deer (<i>Cervus elaphus</i>) in Patagonia, Argentina
dc.creator.none.fl_str_mv Chang Reissig, Elizabeth
Moré, Gastón Andrés
Massone, Adriana Raquel
Uzal, Francisco A.
author Chang Reissig, Elizabeth
author_facet Chang Reissig, Elizabeth
Moré, Gastón Andrés
Massone, Adriana Raquel
Uzal, Francisco A.
author_role author
author2 Moré, Gastón Andrés
Massone, Adriana Raquel
Uzal, Francisco A.
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Sarcocystis taeniata
Red deer
Argentinean nonnative ungulate
TEM
Sequencing
topic Ciencias Veterinarias
Sarcocystis taeniata
Red deer
Argentinean nonnative ungulate
TEM
Sequencing
dc.description.none.fl_txt_mv Sarcocystis spp. are protozoan parasites with a heteroxenous life cycle, which produce cysts in the muscle of herbivorous animals. In these animal species, sarcocystosis is frequently asymptomatic, although it may occur with high prevalence. Seven Sarcocystis spp. have been described in red deer (Cervus elephus). The aim of this study was to determine the prevalence of sarcocystosis, and to perform the morphological and molecular characterization of Sarcocystis spp. found in wild red deer of the Nahuel Huapi National Park (NHNP), Patagonia, Argentina. Full necropsies of 62 red deer killed by hunters in the NHNP and neighboring areas were performed. Samples of heart and skeletal muscle were examined histologically and selected samples were also examined by transmission electron microscopy (TEM), PCR and sequencing. Sarcocystis spp. thin walled cysts were detected in 62 % (38/62) of heart, and in 22 % (3/14) of skeletal muscle samples examined histologically. TEM revealed a smooth and thin cyst wall (≤1 μm), with scarce and separated ribbon-like protrusions. A total of three partial and one full 18S ribosomal RNA (rRNA) gene sequences were obtained, and showed the highest identity (≥99 %) with Sarcocystis taeniata, a species described in moose (Alces alces). The morphological and molecular results indicate that red deer in Argentina are frequently infected with S. taeniata, a species for which the definitive host is unknown. The present results also confirm that Sarcocystis spp. using cervids as intermediate host are not host-specific. Further studies are needed to improve the epidemiological knowledge of Sarcocystosis in red deer.
Facultad de Ciencias Veterinarias
description Sarcocystis spp. are protozoan parasites with a heteroxenous life cycle, which produce cysts in the muscle of herbivorous animals. In these animal species, sarcocystosis is frequently asymptomatic, although it may occur with high prevalence. Seven Sarcocystis spp. have been described in red deer (Cervus elephus). The aim of this study was to determine the prevalence of sarcocystosis, and to perform the morphological and molecular characterization of Sarcocystis spp. found in wild red deer of the Nahuel Huapi National Park (NHNP), Patagonia, Argentina. Full necropsies of 62 red deer killed by hunters in the NHNP and neighboring areas were performed. Samples of heart and skeletal muscle were examined histologically and selected samples were also examined by transmission electron microscopy (TEM), PCR and sequencing. Sarcocystis spp. thin walled cysts were detected in 62 % (38/62) of heart, and in 22 % (3/14) of skeletal muscle samples examined histologically. TEM revealed a smooth and thin cyst wall (≤1 μm), with scarce and separated ribbon-like protrusions. A total of three partial and one full 18S ribosomal RNA (rRNA) gene sequences were obtained, and showed the highest identity (≥99 %) with Sarcocystis taeniata, a species described in moose (Alces alces). The morphological and molecular results indicate that red deer in Argentina are frequently infected with S. taeniata, a species for which the definitive host is unknown. The present results also confirm that Sarcocystis spp. using cervids as intermediate host are not host-specific. Further studies are needed to improve the epidemiological knowledge of Sarcocystosis in red deer.
publishDate 2016
dc.date.none.fl_str_mv 2016-01-16
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/139537
url http://sedici.unlp.edu.ar/handle/10915/139537
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1432-1955
info:eu-repo/semantics/altIdentifier/issn/0932-0113
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00436-016-4915-7
info:eu-repo/semantics/altIdentifier/pmid/26779923
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
1773-1778
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1844616204749111296
score 13.070432

Publicaciones similares