Alterations of the antioxidant enzyme activities are not general characteristics of the colonization process by arbuscular mycorrhizal fungi

Autores
Rodríguez, Yakelin; Vierheilig, Horst; Mazorra, Luis M.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Antioxidant system is involved in arbuscular mycorrhizal symbiosis, but its role during the colonization process is still poorly understood. To gain new insights into the role of antioxidant system during root colonization by arbuscular mycorrhizal fungi, the activities of key antioxidant enzymes were evaluated in tomato (Solanum lycopersicum L.) roots inoculated with six strains of different genera and species: two Glomus mosseae, Glomus cubense, Glomus intraradices, Glomus sp. and Acaulospora scrobiculata. Glomus cubense and A. scrobiculata strains reached the highest infectivity levels with maximum values of colonization frequency and intensity of 29-10.88% and 18-9.20%, respectively; G. mosseae strains showed an intermediate infectivity, both with 15% of colonization frequency and maximum intensities of 7.64- 7.06%, respectively; while the infectivity levels of Glomus sp. and G. intraradices strains were the lowest with colonization frequency- 13% and intensities- 5.07 and 5.41, respectively. Some activity patterns of peroxidase, superoxide dismutase, and polyphenol oxidase enzymes were not specific for early or late colonization stages neither for the colonization level and type of strain. However, a unique superoxide dismutase-band presents at early colonization and the low level of guaiacolperoxidase activity at later stages presents in all inoculated roots indicate that these antioxidant responses are independent of colonization degree and strain. Taking together, our data suggest that alterations of the antioxidant enzyme activities are not general characteristics of the colonization process by arbuscular mycorrhizal fungi, probably having the key role on those responses the specific feature of each strain rather than colonization per se.
El sistema antioxidante está involucrado en la simbiosis micorrízico-arbuscular, pero su rol durante el proceso de colonización es aún escasamente comprendido. Para esclarecer el papel del sistema antioxidante durante la colonización radical por los hongos micorrízicos arbusculares, se evaluaron las actividades de enzimas antioxidantes claves en raíces de tomate (Solanum lycopersicum L.) inoculadas con seis cepas diferentes: dos Glomus mosseae, Glomus cubense, Glomus intraradices, Glomus sp. y A. scrobiculata. Las cepas G. cubense y A. scrobiculata alcanzaron los niveles de infectividad superiores con valores máximos de colonización e intensidad de 29-10,88% y 18-9,20%, respectivamente; las cepas G. mosseae mostraron una infectividad intermedia, ambas con 15% de colonización e intensidades máximas de 7,64-7,06%, respectivamente; mientras que los niveles de infectividad de las cepas Glomus sp. y G. intraradices fueron inferiores con colonización del 13% e intensidades de 5,07 y 5,41, respectivamente. Algunos patrones de actividad de las enzimas peroxidasa, superóxido dismutasa y polifenol oxidasa no resultaron específicos para los niveles y estadios de colonización, temprano o tardío, ni tipo de cepa. No obstante, la única banda de superóxido dismutasa presente en la colonización temprana y el bajo nivel de actividad guayacol-peroxidasa en estadio tardío presente en todos los tratamientos micorrizados indican que estas respuestas antioxidantes son independientes de la cepa y el grado de colonización. En conjunto, los datos sugieren que las alteraciones de las actividades enzimáticas antioxidantes no son características generales del proceso de colonización por los hongos micorrízicos arbusculares, teniendo probablemente el rol clave sobre estas respuestas las características específicas de cada cepa más que la colonización per se.
Instituto de Fisiología Vegetal, Universidad Nacional de La Plata
Materia
Ciencias Agrarias
Colonization intensity
Peroxidases
Polyphenol oxidases
Solanum lycopersicum
Superoxide dismutase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/84413

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oai_identifier_str oai:sedici.unlp.edu.ar:10915/84413
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Alterations of the antioxidant enzyme activities are not general characteristics of the colonization process by arbuscular mycorrhizal fungiAlteraciones de las actividades de enzimas antioxidantes no son características generales del proceso de colonización por hongos micorrízicos arbuscularesRodríguez, YakelinVierheilig, HorstMazorra, Luis M.Ciencias AgrariasColonization intensityPeroxidasesPolyphenol oxidasesSolanum lycopersicumSuperoxide dismutaseAntioxidant system is involved in arbuscular mycorrhizal symbiosis, but its role during the colonization process is still poorly understood. To gain new insights into the role of antioxidant system during root colonization by arbuscular mycorrhizal fungi, the activities of key antioxidant enzymes were evaluated in tomato (Solanum lycopersicum L.) roots inoculated with six strains of different genera and species: two Glomus mosseae, Glomus cubense, Glomus intraradices, Glomus sp. and Acaulospora scrobiculata. Glomus cubense and A. scrobiculata strains reached the highest infectivity levels with maximum values of colonization frequency and intensity of 29-10.88% and 18-9.20%, respectively; G. mosseae strains showed an intermediate infectivity, both with 15% of colonization frequency and maximum intensities of 7.64- 7.06%, respectively; while the infectivity levels of Glomus sp. and G. intraradices strains were the lowest with colonization frequency- 13% and intensities- 5.07 and 5.41, respectively. Some activity patterns of peroxidase, superoxide dismutase, and polyphenol oxidase enzymes were not specific for early or late colonization stages neither for the colonization level and type of strain. However, a unique superoxide dismutase-band presents at early colonization and the low level of guaiacolperoxidase activity at later stages presents in all inoculated roots indicate that these antioxidant responses are independent of colonization degree and strain. Taking together, our data suggest that alterations of the antioxidant enzyme activities are not general characteristics of the colonization process by arbuscular mycorrhizal fungi, probably having the key role on those responses the specific feature of each strain rather than colonization per se.El sistema antioxidante está involucrado en la simbiosis micorrízico-arbuscular, pero su rol durante el proceso de colonización es aún escasamente comprendido. Para esclarecer el papel del sistema antioxidante durante la colonización radical por los hongos micorrízicos arbusculares, se evaluaron las actividades de enzimas antioxidantes claves en raíces de tomate (Solanum lycopersicum L.) inoculadas con seis cepas diferentes: dos Glomus mosseae, Glomus cubense, Glomus intraradices, Glomus sp. y A. scrobiculata. Las cepas G. cubense y A. scrobiculata alcanzaron los niveles de infectividad superiores con valores máximos de colonización e intensidad de 29-10,88% y 18-9,20%, respectivamente; las cepas G. mosseae mostraron una infectividad intermedia, ambas con 15% de colonización e intensidades máximas de 7,64-7,06%, respectivamente; mientras que los niveles de infectividad de las cepas Glomus sp. y G. intraradices fueron inferiores con colonización del 13% e intensidades de 5,07 y 5,41, respectivamente. Algunos patrones de actividad de las enzimas peroxidasa, superóxido dismutasa y polifenol oxidasa no resultaron específicos para los niveles y estadios de colonización, temprano o tardío, ni tipo de cepa. No obstante, la única banda de superóxido dismutasa presente en la colonización temprana y el bajo nivel de actividad guayacol-peroxidasa en estadio tardío presente en todos los tratamientos micorrizados indican que estas respuestas antioxidantes son independientes de la cepa y el grado de colonización. En conjunto, los datos sugieren que las alteraciones de las actividades enzimáticas antioxidantes no son características generales del proceso de colonización por los hongos micorrízicos arbusculares, teniendo probablemente el rol clave sobre estas respuestas las características específicas de cada cepa más que la colonización per se.Instituto de Fisiología Vegetal, Universidad Nacional de La Plata2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf411-418http://sedici.unlp.edu.ar/handle/10915/84413enginfo:eu-repo/semantics/altIdentifier/issn/0718-5820info:eu-repo/semantics/altIdentifier/doi/10.4067/S0718-58392012000300016info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:16:05Zoai:sedici.unlp.edu.ar:10915/84413Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:16:05.57SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Alterations of the antioxidant enzyme activities are not general characteristics of the colonization process by arbuscular mycorrhizal fungi
Alteraciones de las actividades de enzimas antioxidantes no son características generales del proceso de colonización por hongos micorrízicos arbusculares
title Alterations of the antioxidant enzyme activities are not general characteristics of the colonization process by arbuscular mycorrhizal fungi
spellingShingle Alterations of the antioxidant enzyme activities are not general characteristics of the colonization process by arbuscular mycorrhizal fungi
Rodríguez, Yakelin
Ciencias Agrarias
Colonization intensity
Peroxidases
Polyphenol oxidases
Solanum lycopersicum
Superoxide dismutase
title_short Alterations of the antioxidant enzyme activities are not general characteristics of the colonization process by arbuscular mycorrhizal fungi
title_full Alterations of the antioxidant enzyme activities are not general characteristics of the colonization process by arbuscular mycorrhizal fungi
title_fullStr Alterations of the antioxidant enzyme activities are not general characteristics of the colonization process by arbuscular mycorrhizal fungi
title_full_unstemmed Alterations of the antioxidant enzyme activities are not general characteristics of the colonization process by arbuscular mycorrhizal fungi
title_sort Alterations of the antioxidant enzyme activities are not general characteristics of the colonization process by arbuscular mycorrhizal fungi
dc.creator.none.fl_str_mv Rodríguez, Yakelin
Vierheilig, Horst
Mazorra, Luis M.
author Rodríguez, Yakelin
author_facet Rodríguez, Yakelin
Vierheilig, Horst
Mazorra, Luis M.
author_role author
author2 Vierheilig, Horst
Mazorra, Luis M.
author2_role author
author
dc.subject.none.fl_str_mv Ciencias Agrarias
Colonization intensity
Peroxidases
Polyphenol oxidases
Solanum lycopersicum
Superoxide dismutase
topic Ciencias Agrarias
Colonization intensity
Peroxidases
Polyphenol oxidases
Solanum lycopersicum
Superoxide dismutase
dc.description.none.fl_txt_mv Antioxidant system is involved in arbuscular mycorrhizal symbiosis, but its role during the colonization process is still poorly understood. To gain new insights into the role of antioxidant system during root colonization by arbuscular mycorrhizal fungi, the activities of key antioxidant enzymes were evaluated in tomato (Solanum lycopersicum L.) roots inoculated with six strains of different genera and species: two Glomus mosseae, Glomus cubense, Glomus intraradices, Glomus sp. and Acaulospora scrobiculata. Glomus cubense and A. scrobiculata strains reached the highest infectivity levels with maximum values of colonization frequency and intensity of 29-10.88% and 18-9.20%, respectively; G. mosseae strains showed an intermediate infectivity, both with 15% of colonization frequency and maximum intensities of 7.64- 7.06%, respectively; while the infectivity levels of Glomus sp. and G. intraradices strains were the lowest with colonization frequency- 13% and intensities- 5.07 and 5.41, respectively. Some activity patterns of peroxidase, superoxide dismutase, and polyphenol oxidase enzymes were not specific for early or late colonization stages neither for the colonization level and type of strain. However, a unique superoxide dismutase-band presents at early colonization and the low level of guaiacolperoxidase activity at later stages presents in all inoculated roots indicate that these antioxidant responses are independent of colonization degree and strain. Taking together, our data suggest that alterations of the antioxidant enzyme activities are not general characteristics of the colonization process by arbuscular mycorrhizal fungi, probably having the key role on those responses the specific feature of each strain rather than colonization per se.
El sistema antioxidante está involucrado en la simbiosis micorrízico-arbuscular, pero su rol durante el proceso de colonización es aún escasamente comprendido. Para esclarecer el papel del sistema antioxidante durante la colonización radical por los hongos micorrízicos arbusculares, se evaluaron las actividades de enzimas antioxidantes claves en raíces de tomate (Solanum lycopersicum L.) inoculadas con seis cepas diferentes: dos Glomus mosseae, Glomus cubense, Glomus intraradices, Glomus sp. y A. scrobiculata. Las cepas G. cubense y A. scrobiculata alcanzaron los niveles de infectividad superiores con valores máximos de colonización e intensidad de 29-10,88% y 18-9,20%, respectivamente; las cepas G. mosseae mostraron una infectividad intermedia, ambas con 15% de colonización e intensidades máximas de 7,64-7,06%, respectivamente; mientras que los niveles de infectividad de las cepas Glomus sp. y G. intraradices fueron inferiores con colonización del 13% e intensidades de 5,07 y 5,41, respectivamente. Algunos patrones de actividad de las enzimas peroxidasa, superóxido dismutasa y polifenol oxidasa no resultaron específicos para los niveles y estadios de colonización, temprano o tardío, ni tipo de cepa. No obstante, la única banda de superóxido dismutasa presente en la colonización temprana y el bajo nivel de actividad guayacol-peroxidasa en estadio tardío presente en todos los tratamientos micorrizados indican que estas respuestas antioxidantes son independientes de la cepa y el grado de colonización. En conjunto, los datos sugieren que las alteraciones de las actividades enzimáticas antioxidantes no son características generales del proceso de colonización por los hongos micorrízicos arbusculares, teniendo probablemente el rol clave sobre estas respuestas las características específicas de cada cepa más que la colonización per se.
Instituto de Fisiología Vegetal, Universidad Nacional de La Plata
description Antioxidant system is involved in arbuscular mycorrhizal symbiosis, but its role during the colonization process is still poorly understood. To gain new insights into the role of antioxidant system during root colonization by arbuscular mycorrhizal fungi, the activities of key antioxidant enzymes were evaluated in tomato (Solanum lycopersicum L.) roots inoculated with six strains of different genera and species: two Glomus mosseae, Glomus cubense, Glomus intraradices, Glomus sp. and Acaulospora scrobiculata. Glomus cubense and A. scrobiculata strains reached the highest infectivity levels with maximum values of colonization frequency and intensity of 29-10.88% and 18-9.20%, respectively; G. mosseae strains showed an intermediate infectivity, both with 15% of colonization frequency and maximum intensities of 7.64- 7.06%, respectively; while the infectivity levels of Glomus sp. and G. intraradices strains were the lowest with colonization frequency- 13% and intensities- 5.07 and 5.41, respectively. Some activity patterns of peroxidase, superoxide dismutase, and polyphenol oxidase enzymes were not specific for early or late colonization stages neither for the colonization level and type of strain. However, a unique superoxide dismutase-band presents at early colonization and the low level of guaiacolperoxidase activity at later stages presents in all inoculated roots indicate that these antioxidant responses are independent of colonization degree and strain. Taking together, our data suggest that alterations of the antioxidant enzyme activities are not general characteristics of the colonization process by arbuscular mycorrhizal fungi, probably having the key role on those responses the specific feature of each strain rather than colonization per se.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/84413
url http://sedici.unlp.edu.ar/handle/10915/84413
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0718-5820
info:eu-repo/semantics/altIdentifier/doi/10.4067/S0718-58392012000300016
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
411-418
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instname:Universidad Nacional de La Plata
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instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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