Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against <i>Bordetella pertussis</i> Infection

Autores
Olivera, María Noelia; Castuma, Celina E.; Hozbor, Daniela Flavia; Gaillard, María Emilia; Rumbo, Martín; Gómez, Ricardo Martín
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study examined the immunogenic properties of the fusion protein fimbria 2 of Bordetella pertussis (Fim2)—cholera toxin B subunit (CTB) in the intranasal murine model of infection. To this end B. pertussis Fim2 coding sequence was cloned downstream of the cholera toxin B subunit coding sequence.Theexpression and assembly of the fusion protein into pentameric structures (CTBFim2) were evaluated by SDS-PAGE and monosialotetrahexosylgaglioside (GM1-ganglioside) enzyme-linked immunosorbent assay (ELISA). To evaluate the protective capacity of CTB-Fim2, an intraperitoneal or intranasal mouse immunization schedule was performed with 50 μg of CTB-Fim2. Recombinant (rFim2) or purified (BpFim2) Fim2, CTB, and phosphate-buffered saline (PBS) were used as controls. The results showed that mice immunized with BpFim2 or CTB-Fim2 intraperitoneally or intranasally presented a significant reduction in bacterial lung counts compared to control groups (P < 0.01 or P < 0.001, resp.). Moreover, intranasal immunization with CTB-Fim2 induced significant levels of Fim2-specific IgG in serum and bronchoalveolar lavage (BAL) and Fim2-specific IgA in BAL. Analysis of IgG isotypes and cytokines mRNA levels showed that CTB-Fim2 results in a mixed Th1/Th2 (T-helper) response. The data presented here provide support for CTB-Fim2 as a promising recombinant antigen against Bordetella pertussis infection.
Facultad de Ciencias Exactas
Materia
Ciencias Exactas
Bordetella pertussis
Inmunización
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/68057
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/68057
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against <i>Bordetella pertussis</i> InfectionOlivera, María NoeliaCastuma, Celina E.Hozbor, Daniela FlaviaGaillard, María EmiliaRumbo, MartínGómez, Ricardo MartínCiencias ExactasBordetella pertussisInmunizaciónThis study examined the immunogenic properties of the fusion protein fimbria 2 of Bordetella pertussis (Fim2)—cholera toxin B subunit (CTB) in the intranasal murine model of infection. To this end B. pertussis Fim2 coding sequence was cloned downstream of the cholera toxin B subunit coding sequence.Theexpression and assembly of the fusion protein into pentameric structures (CTBFim2) were evaluated by SDS-PAGE and monosialotetrahexosylgaglioside (GM1-ganglioside) enzyme-linked immunosorbent assay (ELISA). To evaluate the protective capacity of CTB-Fim2, an intraperitoneal or intranasal mouse immunization schedule was performed with 50 μg of CTB-Fim2. Recombinant (rFim2) or purified (BpFim2) Fim2, CTB, and phosphate-buffered saline (PBS) were used as controls. The results showed that mice immunized with BpFim2 or CTB-Fim2 intraperitoneally or intranasally presented a significant reduction in bacterial lung counts compared to control groups (P < 0.01 or P < 0.001, resp.). Moreover, intranasal immunization with CTB-Fim2 induced significant levels of Fim2-specific IgG in serum and bronchoalveolar lavage (BAL) and Fim2-specific IgA in BAL. Analysis of IgG isotypes and cytokines mRNA levels showed that CTB-Fim2 results in a mixed Th1/Th2 (T-helper) response. The data presented here provide support for CTB-Fim2 as a promising recombinant antigen against Bordetella pertussis infection.Facultad de Ciencias Exactas2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/68057enginfo:eu-repo/semantics/altIdentifier/issn/2314-6141info:eu-repo/semantics/altIdentifier/doi/10.1155/2014/421486info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:10:30Zoai:sedici.unlp.edu.ar:10915/68057Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:10:30.744SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against <i>Bordetella pertussis</i> Infection
title Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against <i>Bordetella pertussis</i> Infection
spellingShingle Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against <i>Bordetella pertussis</i> Infection
Olivera, María Noelia
Ciencias Exactas
Bordetella pertussis
Inmunización
title_short Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against <i>Bordetella pertussis</i> Infection
title_full Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against <i>Bordetella pertussis</i> Infection
title_fullStr Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against <i>Bordetella pertussis</i> Infection
title_full_unstemmed Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against <i>Bordetella pertussis</i> Infection
title_sort Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against <i>Bordetella pertussis</i> Infection
dc.creator.none.fl_str_mv Olivera, María Noelia
Castuma, Celina E.
Hozbor, Daniela Flavia
Gaillard, María Emilia
Rumbo, Martín
Gómez, Ricardo Martín
author Olivera, María Noelia
author_facet Olivera, María Noelia
Castuma, Celina E.
Hozbor, Daniela Flavia
Gaillard, María Emilia
Rumbo, Martín
Gómez, Ricardo Martín
author_role author
author2 Castuma, Celina E.
Hozbor, Daniela Flavia
Gaillard, María Emilia
Rumbo, Martín
Gómez, Ricardo Martín
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Bordetella pertussis
Inmunización
topic Ciencias Exactas
Bordetella pertussis
Inmunización
dc.description.none.fl_txt_mv This study examined the immunogenic properties of the fusion protein fimbria 2 of Bordetella pertussis (Fim2)—cholera toxin B subunit (CTB) in the intranasal murine model of infection. To this end B. pertussis Fim2 coding sequence was cloned downstream of the cholera toxin B subunit coding sequence.Theexpression and assembly of the fusion protein into pentameric structures (CTBFim2) were evaluated by SDS-PAGE and monosialotetrahexosylgaglioside (GM1-ganglioside) enzyme-linked immunosorbent assay (ELISA). To evaluate the protective capacity of CTB-Fim2, an intraperitoneal or intranasal mouse immunization schedule was performed with 50 μg of CTB-Fim2. Recombinant (rFim2) or purified (BpFim2) Fim2, CTB, and phosphate-buffered saline (PBS) were used as controls. The results showed that mice immunized with BpFim2 or CTB-Fim2 intraperitoneally or intranasally presented a significant reduction in bacterial lung counts compared to control groups (P < 0.01 or P < 0.001, resp.). Moreover, intranasal immunization with CTB-Fim2 induced significant levels of Fim2-specific IgG in serum and bronchoalveolar lavage (BAL) and Fim2-specific IgA in BAL. Analysis of IgG isotypes and cytokines mRNA levels showed that CTB-Fim2 results in a mixed Th1/Th2 (T-helper) response. The data presented here provide support for CTB-Fim2 as a promising recombinant antigen against Bordetella pertussis infection.
Facultad de Ciencias Exactas
description This study examined the immunogenic properties of the fusion protein fimbria 2 of Bordetella pertussis (Fim2)—cholera toxin B subunit (CTB) in the intranasal murine model of infection. To this end B. pertussis Fim2 coding sequence was cloned downstream of the cholera toxin B subunit coding sequence.Theexpression and assembly of the fusion protein into pentameric structures (CTBFim2) were evaluated by SDS-PAGE and monosialotetrahexosylgaglioside (GM1-ganglioside) enzyme-linked immunosorbent assay (ELISA). To evaluate the protective capacity of CTB-Fim2, an intraperitoneal or intranasal mouse immunization schedule was performed with 50 μg of CTB-Fim2. Recombinant (rFim2) or purified (BpFim2) Fim2, CTB, and phosphate-buffered saline (PBS) were used as controls. The results showed that mice immunized with BpFim2 or CTB-Fim2 intraperitoneally or intranasally presented a significant reduction in bacterial lung counts compared to control groups (P < 0.01 or P < 0.001, resp.). Moreover, intranasal immunization with CTB-Fim2 induced significant levels of Fim2-specific IgG in serum and bronchoalveolar lavage (BAL) and Fim2-specific IgA in BAL. Analysis of IgG isotypes and cytokines mRNA levels showed that CTB-Fim2 results in a mixed Th1/Th2 (T-helper) response. The data presented here provide support for CTB-Fim2 as a promising recombinant antigen against Bordetella pertussis infection.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/68057
url http://sedici.unlp.edu.ar/handle/10915/68057
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2314-6141
info:eu-repo/semantics/altIdentifier/doi/10.1155/2014/421486
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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