Role of Ca2+-calmodulin dependent phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists

Autores
Vittone, Leticia Beatriz; Mundiña-Weilenmann, Cecilia; Chiappe de Cingolani, Gladys Ethel; Mattiazzi, Alicia Ramona
Año de publicación
1993
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The role of the Ca2+-calmodulin dependent pathway of phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists was studied in isolated perfused rat heart. Administration of the calmodulin antagonist W7 or lowering [Ca]0 from 1.35 mM (control) to 0.25 mM, were used as experimental tools to inhibit the Ca2+-calmodulin dependent protein kinase activity. 3×10−8 M isoproterenol increased cAMP levels from 0.613±0.109 pmol/mg wet weight to 1.581±0.123, phospholamban phosphorylation from 36±6 pmol32P/mg protein to 277±26 and decreased time to half relaxation (t1/2) from 61±2 msec to 39±2. Simultaneous perfusion of isoproterenol with 10−6 M W7, decreased phospholamban phosphorylation to 170±23 and prolongated t1/2 to 47±3 but did not affect the increase either in cAMP levels or myocardial contractility produced by isoproterenol. Similar effects on phospholamban phosphorylation and myocardial relaxation were obtained when isoproterenol was perfused in low [Ca]0. Low [Ca]0 did not affect the increase in cAMP elicited by isoproterenol but offset the positive inotropic effect of the β-agonist.
Centro de Investigaciones Cardiovasculares
Materia
Ciencias Médicas
myocardial relaxation
phospholamban
Ca2+-calmodulin dependent phosphorylation
W7
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/140836
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/140836
network_acronym_str SEDICI
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network_name_str SEDICI (UNLP)
spelling Role of Ca2+-calmodulin dependent phospholamban phosphorylation on the relaxant effect of β-adrenergic agonistsVittone, Leticia BeatrizMundiña-Weilenmann, CeciliaChiappe de Cingolani, Gladys EthelMattiazzi, Alicia RamonaCiencias Médicasmyocardial relaxationphospholambanCa2+-calmodulin dependent phosphorylationW7The role of the Ca2+-calmodulin dependent pathway of phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists was studied in isolated perfused rat heart. Administration of the calmodulin antagonist W7 or lowering [Ca]0 from 1.35 mM (control) to 0.25 mM, were used as experimental tools to inhibit the Ca2+-calmodulin dependent protein kinase activity. 3×10−8 M isoproterenol increased cAMP levels from 0.613±0.109 pmol/mg wet weight to 1.581±0.123, phospholamban phosphorylation from 36±6 pmol32P/mg protein to 277±26 and decreased time to half relaxation (t1/2) from 61±2 msec to 39±2. Simultaneous perfusion of isoproterenol with 10−6 M W7, decreased phospholamban phosphorylation to 170±23 and prolongated t1/2 to 47±3 but did not affect the increase either in cAMP levels or myocardial contractility produced by isoproterenol. Similar effects on phospholamban phosphorylation and myocardial relaxation were obtained when isoproterenol was perfused in low [Ca]0. Low [Ca]0 did not affect the increase in cAMP elicited by isoproterenol but offset the positive inotropic effect of the β-agonist.Centro de Investigaciones Cardiovasculares1993-07-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf33-42http://sedici.unlp.edu.ar/handle/10915/140836enginfo:eu-repo/semantics/altIdentifier/issn/0300-8177info:eu-repo/semantics/altIdentifier/issn/1573-4919info:eu-repo/semantics/altIdentifier/doi/10.1007/bf01096379info:eu-repo/semantics/altIdentifier/pmid/8232275info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:31:53Zoai:sedici.unlp.edu.ar:10915/140836Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:31:53.404SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Role of Ca2+-calmodulin dependent phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists
title Role of Ca2+-calmodulin dependent phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists
spellingShingle Role of Ca2+-calmodulin dependent phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists
Vittone, Leticia Beatriz
Ciencias Médicas
myocardial relaxation
phospholamban
Ca2+-calmodulin dependent phosphorylation
W7
title_short Role of Ca2+-calmodulin dependent phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists
title_full Role of Ca2+-calmodulin dependent phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists
title_fullStr Role of Ca2+-calmodulin dependent phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists
title_full_unstemmed Role of Ca2+-calmodulin dependent phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists
title_sort Role of Ca2+-calmodulin dependent phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists
dc.creator.none.fl_str_mv Vittone, Leticia Beatriz
Mundiña-Weilenmann, Cecilia
Chiappe de Cingolani, Gladys Ethel
Mattiazzi, Alicia Ramona
author Vittone, Leticia Beatriz
author_facet Vittone, Leticia Beatriz
Mundiña-Weilenmann, Cecilia
Chiappe de Cingolani, Gladys Ethel
Mattiazzi, Alicia Ramona
author_role author
author2 Mundiña-Weilenmann, Cecilia
Chiappe de Cingolani, Gladys Ethel
Mattiazzi, Alicia Ramona
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Médicas
myocardial relaxation
phospholamban
Ca2+-calmodulin dependent phosphorylation
W7
topic Ciencias Médicas
myocardial relaxation
phospholamban
Ca2+-calmodulin dependent phosphorylation
W7
dc.description.none.fl_txt_mv The role of the Ca2+-calmodulin dependent pathway of phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists was studied in isolated perfused rat heart. Administration of the calmodulin antagonist W7 or lowering [Ca]0 from 1.35 mM (control) to 0.25 mM, were used as experimental tools to inhibit the Ca2+-calmodulin dependent protein kinase activity. 3×10−8 M isoproterenol increased cAMP levels from 0.613±0.109 pmol/mg wet weight to 1.581±0.123, phospholamban phosphorylation from 36±6 pmol32P/mg protein to 277±26 and decreased time to half relaxation (t1/2) from 61±2 msec to 39±2. Simultaneous perfusion of isoproterenol with 10−6 M W7, decreased phospholamban phosphorylation to 170±23 and prolongated t1/2 to 47±3 but did not affect the increase either in cAMP levels or myocardial contractility produced by isoproterenol. Similar effects on phospholamban phosphorylation and myocardial relaxation were obtained when isoproterenol was perfused in low [Ca]0. Low [Ca]0 did not affect the increase in cAMP elicited by isoproterenol but offset the positive inotropic effect of the β-agonist.
Centro de Investigaciones Cardiovasculares
description The role of the Ca2+-calmodulin dependent pathway of phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists was studied in isolated perfused rat heart. Administration of the calmodulin antagonist W7 or lowering [Ca]0 from 1.35 mM (control) to 0.25 mM, were used as experimental tools to inhibit the Ca2+-calmodulin dependent protein kinase activity. 3×10−8 M isoproterenol increased cAMP levels from 0.613±0.109 pmol/mg wet weight to 1.581±0.123, phospholamban phosphorylation from 36±6 pmol32P/mg protein to 277±26 and decreased time to half relaxation (t1/2) from 61±2 msec to 39±2. Simultaneous perfusion of isoproterenol with 10−6 M W7, decreased phospholamban phosphorylation to 170±23 and prolongated t1/2 to 47±3 but did not affect the increase either in cAMP levels or myocardial contractility produced by isoproterenol. Similar effects on phospholamban phosphorylation and myocardial relaxation were obtained when isoproterenol was perfused in low [Ca]0. Low [Ca]0 did not affect the increase in cAMP elicited by isoproterenol but offset the positive inotropic effect of the β-agonist.
publishDate 1993
dc.date.none.fl_str_mv 1993-07-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/140836
url http://sedici.unlp.edu.ar/handle/10915/140836
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0300-8177
info:eu-repo/semantics/altIdentifier/issn/1573-4919
info:eu-repo/semantics/altIdentifier/doi/10.1007/bf01096379
info:eu-repo/semantics/altIdentifier/pmid/8232275
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
33-42
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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