Humulus lupulus Promoting Osteoblast Activity and Bone Integrity: Effects and Mechanisms
- Autores
- Wanionok, Nahuel Ezequiel; Colareda, Germán Andrés; Fernández, Juan Manuel
- Año de publicación
- 2025
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Osteoporosis is characterized by an imbalance between bone formation and resorption, leading to decreased bone mass and an increased fracture risk, mainly associated with aging. Current treatments include anti-resorptive and anabolic drugs. However, these often have side effects, leadingmany patients to seek natural biological alternatives. We have demonstrated previously that hops extract, rich in compounds with estrogenic activity classified as phytoestrogens, exerts osteogenic effects by promoting the osteoblastic differentiation of bone marrowstemcells (BMSCs)while inhibiting osteoclast activity in vitro. In our study, youngmale Sprague Dawley rats were randomized into two groups: one received hops extract (LPL, 1% w/v in drinking water) for two months, and the other drank water alone (C). The rats were euthanized, and their femurs were dissected and processed for static histomorphometry and bone biomechanics. Additionally, BMSCs were isolated from the humeri to evaluate their osteogenic potential. Our result demonstrated that LPL treatment enhanced the osteogenic potential of humeral BMSCs in ex vivo assays, upregulating osteogenic genes and downregulating proresorptivemarkers. These findings correlated with improved femoral bonemicroarchitecture and biomechanical parameters. In conclusion, a two-month treatment with LPL enhanced the osteogenic capacity of BMSCs, improving bonemicroarchitecture and biomechanical properties. These results suggest its potential as a natural alternative for promoting bone health.
Laboratorio de Investigación en Osteopatías y Metabolismo Mineral - Materia
-
Biología
osteoblast
bone marrow stem cells
bone microarchitecture
biomechanics
hops
phytoestrogens - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/181453
Ver los metadatos del registro completo
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Humulus lupulus Promoting Osteoblast Activity and Bone Integrity: Effects and MechanismsWanionok, Nahuel EzequielColareda, Germán AndrésFernández, Juan ManuelBiologíaosteoblastbone marrow stem cellsbone microarchitecturebiomechanicshopsphytoestrogensOsteoporosis is characterized by an imbalance between bone formation and resorption, leading to decreased bone mass and an increased fracture risk, mainly associated with aging. Current treatments include anti-resorptive and anabolic drugs. However, these often have side effects, leadingmany patients to seek natural biological alternatives. We have demonstrated previously that hops extract, rich in compounds with estrogenic activity classified as phytoestrogens, exerts osteogenic effects by promoting the osteoblastic differentiation of bone marrowstemcells (BMSCs)while inhibiting osteoclast activity in vitro. In our study, youngmale Sprague Dawley rats were randomized into two groups: one received hops extract (LPL, 1% w/v in drinking water) for two months, and the other drank water alone (C). The rats were euthanized, and their femurs were dissected and processed for static histomorphometry and bone biomechanics. Additionally, BMSCs were isolated from the humeri to evaluate their osteogenic potential. Our result demonstrated that LPL treatment enhanced the osteogenic potential of humeral BMSCs in ex vivo assays, upregulating osteogenic genes and downregulating proresorptivemarkers. These findings correlated with improved femoral bonemicroarchitecture and biomechanical parameters. In conclusion, a two-month treatment with LPL enhanced the osteogenic capacity of BMSCs, improving bonemicroarchitecture and biomechanical properties. These results suggest its potential as a natural alternative for promoting bone health.Laboratorio de Investigación en Osteopatías y Metabolismo Mineral2025-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/181453enginfo:eu-repo/semantics/altIdentifier/issn/2079-7737info:eu-repo/semantics/altIdentifier/doi/10.3390/biology14050582info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:49:33Zoai:sedici.unlp.edu.ar:10915/181453Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:49:33.931SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Humulus lupulus Promoting Osteoblast Activity and Bone Integrity: Effects and Mechanisms |
title |
Humulus lupulus Promoting Osteoblast Activity and Bone Integrity: Effects and Mechanisms |
spellingShingle |
Humulus lupulus Promoting Osteoblast Activity and Bone Integrity: Effects and Mechanisms Wanionok, Nahuel Ezequiel Biología osteoblast bone marrow stem cells bone microarchitecture biomechanics hops phytoestrogens |
title_short |
Humulus lupulus Promoting Osteoblast Activity and Bone Integrity: Effects and Mechanisms |
title_full |
Humulus lupulus Promoting Osteoblast Activity and Bone Integrity: Effects and Mechanisms |
title_fullStr |
Humulus lupulus Promoting Osteoblast Activity and Bone Integrity: Effects and Mechanisms |
title_full_unstemmed |
Humulus lupulus Promoting Osteoblast Activity and Bone Integrity: Effects and Mechanisms |
title_sort |
Humulus lupulus Promoting Osteoblast Activity and Bone Integrity: Effects and Mechanisms |
dc.creator.none.fl_str_mv |
Wanionok, Nahuel Ezequiel Colareda, Germán Andrés Fernández, Juan Manuel |
author |
Wanionok, Nahuel Ezequiel |
author_facet |
Wanionok, Nahuel Ezequiel Colareda, Germán Andrés Fernández, Juan Manuel |
author_role |
author |
author2 |
Colareda, Germán Andrés Fernández, Juan Manuel |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Biología osteoblast bone marrow stem cells bone microarchitecture biomechanics hops phytoestrogens |
topic |
Biología osteoblast bone marrow stem cells bone microarchitecture biomechanics hops phytoestrogens |
dc.description.none.fl_txt_mv |
Osteoporosis is characterized by an imbalance between bone formation and resorption, leading to decreased bone mass and an increased fracture risk, mainly associated with aging. Current treatments include anti-resorptive and anabolic drugs. However, these often have side effects, leadingmany patients to seek natural biological alternatives. We have demonstrated previously that hops extract, rich in compounds with estrogenic activity classified as phytoestrogens, exerts osteogenic effects by promoting the osteoblastic differentiation of bone marrowstemcells (BMSCs)while inhibiting osteoclast activity in vitro. In our study, youngmale Sprague Dawley rats were randomized into two groups: one received hops extract (LPL, 1% w/v in drinking water) for two months, and the other drank water alone (C). The rats were euthanized, and their femurs were dissected and processed for static histomorphometry and bone biomechanics. Additionally, BMSCs were isolated from the humeri to evaluate their osteogenic potential. Our result demonstrated that LPL treatment enhanced the osteogenic potential of humeral BMSCs in ex vivo assays, upregulating osteogenic genes and downregulating proresorptivemarkers. These findings correlated with improved femoral bonemicroarchitecture and biomechanical parameters. In conclusion, a two-month treatment with LPL enhanced the osteogenic capacity of BMSCs, improving bonemicroarchitecture and biomechanical properties. These results suggest its potential as a natural alternative for promoting bone health. Laboratorio de Investigación en Osteopatías y Metabolismo Mineral |
description |
Osteoporosis is characterized by an imbalance between bone formation and resorption, leading to decreased bone mass and an increased fracture risk, mainly associated with aging. Current treatments include anti-resorptive and anabolic drugs. However, these often have side effects, leadingmany patients to seek natural biological alternatives. We have demonstrated previously that hops extract, rich in compounds with estrogenic activity classified as phytoestrogens, exerts osteogenic effects by promoting the osteoblastic differentiation of bone marrowstemcells (BMSCs)while inhibiting osteoclast activity in vitro. In our study, youngmale Sprague Dawley rats were randomized into two groups: one received hops extract (LPL, 1% w/v in drinking water) for two months, and the other drank water alone (C). The rats were euthanized, and their femurs were dissected and processed for static histomorphometry and bone biomechanics. Additionally, BMSCs were isolated from the humeri to evaluate their osteogenic potential. Our result demonstrated that LPL treatment enhanced the osteogenic potential of humeral BMSCs in ex vivo assays, upregulating osteogenic genes and downregulating proresorptivemarkers. These findings correlated with improved femoral bonemicroarchitecture and biomechanical parameters. In conclusion, a two-month treatment with LPL enhanced the osteogenic capacity of BMSCs, improving bonemicroarchitecture and biomechanical properties. These results suggest its potential as a natural alternative for promoting bone health. |
publishDate |
2025 |
dc.date.none.fl_str_mv |
2025-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
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publishedVersion |
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http://sedici.unlp.edu.ar/handle/10915/181453 |
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http://sedici.unlp.edu.ar/handle/10915/181453 |
dc.language.none.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/altIdentifier/issn/2079-7737 info:eu-repo/semantics/altIdentifier/doi/10.3390/biology14050582 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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openAccess |
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http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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