Humulus lupulus Promoting Osteoblast Activity and Bone Integrity: Effects and Mechanisms

Autores
Wanionok, Nahuel Ezequiel; Colareda, Germán Andrés; Fernández, Juan Manuel
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Osteoporosis is characterized by an imbalance between bone formation and resorption, leading to decreased bone mass and an increased fracture risk, mainly associated with aging. Current treatments include anti-resorptive and anabolic drugs. However, these often have side effects, leadingmany patients to seek natural biological alternatives. We have demonstrated previously that hops extract, rich in compounds with estrogenic activity classified as phytoestrogens, exerts osteogenic effects by promoting the osteoblastic differentiation of bone marrowstemcells (BMSCs)while inhibiting osteoclast activity in vitro. In our study, youngmale Sprague Dawley rats were randomized into two groups: one received hops extract (LPL, 1% w/v in drinking water) for two months, and the other drank water alone (C). The rats were euthanized, and their femurs were dissected and processed for static histomorphometry and bone biomechanics. Additionally, BMSCs were isolated from the humeri to evaluate their osteogenic potential. Our result demonstrated that LPL treatment enhanced the osteogenic potential of humeral BMSCs in ex vivo assays, upregulating osteogenic genes and downregulating proresorptivemarkers. These findings correlated with improved femoral bonemicroarchitecture and biomechanical parameters. In conclusion, a two-month treatment with LPL enhanced the osteogenic capacity of BMSCs, improving bonemicroarchitecture and biomechanical properties. These results suggest its potential as a natural alternative for promoting bone health.
Laboratorio de Investigación en Osteopatías y Metabolismo Mineral
Materia
Biología
osteoblast
bone marrow stem cells
bone microarchitecture
biomechanics
hops
phytoestrogens
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/181453
Acceder
id SEDICI_719137a970e41ce4378fbe5de95d007e
oai_identifier_str oai:sedici.unlp.edu.ar:10915/181453
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Humulus lupulus Promoting Osteoblast Activity and Bone Integrity: Effects and MechanismsWanionok, Nahuel EzequielColareda, Germán AndrésFernández, Juan ManuelBiologíaosteoblastbone marrow stem cellsbone microarchitecturebiomechanicshopsphytoestrogensOsteoporosis is characterized by an imbalance between bone formation and resorption, leading to decreased bone mass and an increased fracture risk, mainly associated with aging. Current treatments include anti-resorptive and anabolic drugs. However, these often have side effects, leadingmany patients to seek natural biological alternatives. We have demonstrated previously that hops extract, rich in compounds with estrogenic activity classified as phytoestrogens, exerts osteogenic effects by promoting the osteoblastic differentiation of bone marrowstemcells (BMSCs)while inhibiting osteoclast activity in vitro. In our study, youngmale Sprague Dawley rats were randomized into two groups: one received hops extract (LPL, 1% w/v in drinking water) for two months, and the other drank water alone (C). The rats were euthanized, and their femurs were dissected and processed for static histomorphometry and bone biomechanics. Additionally, BMSCs were isolated from the humeri to evaluate their osteogenic potential. Our result demonstrated that LPL treatment enhanced the osteogenic potential of humeral BMSCs in ex vivo assays, upregulating osteogenic genes and downregulating proresorptivemarkers. These findings correlated with improved femoral bonemicroarchitecture and biomechanical parameters. In conclusion, a two-month treatment with LPL enhanced the osteogenic capacity of BMSCs, improving bonemicroarchitecture and biomechanical properties. These results suggest its potential as a natural alternative for promoting bone health.Laboratorio de Investigación en Osteopatías y Metabolismo Mineral2025-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/181453enginfo:eu-repo/semantics/altIdentifier/issn/2079-7737info:eu-repo/semantics/altIdentifier/doi/10.3390/biology14050582info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:30:26Zoai:sedici.unlp.edu.ar:10915/181453Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:30:26.471SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Humulus lupulus Promoting Osteoblast Activity and Bone Integrity: Effects and Mechanisms
title Humulus lupulus Promoting Osteoblast Activity and Bone Integrity: Effects and Mechanisms
spellingShingle Humulus lupulus Promoting Osteoblast Activity and Bone Integrity: Effects and Mechanisms
Wanionok, Nahuel Ezequiel
Biología
osteoblast
bone marrow stem cells
bone microarchitecture
biomechanics
hops
phytoestrogens
title_short Humulus lupulus Promoting Osteoblast Activity and Bone Integrity: Effects and Mechanisms
title_full Humulus lupulus Promoting Osteoblast Activity and Bone Integrity: Effects and Mechanisms
title_fullStr Humulus lupulus Promoting Osteoblast Activity and Bone Integrity: Effects and Mechanisms
title_full_unstemmed Humulus lupulus Promoting Osteoblast Activity and Bone Integrity: Effects and Mechanisms
title_sort Humulus lupulus Promoting Osteoblast Activity and Bone Integrity: Effects and Mechanisms
dc.creator.none.fl_str_mv Wanionok, Nahuel Ezequiel
Colareda, Germán Andrés
Fernández, Juan Manuel
author Wanionok, Nahuel Ezequiel
author_facet Wanionok, Nahuel Ezequiel
Colareda, Germán Andrés
Fernández, Juan Manuel
author_role author
author2 Colareda, Germán Andrés
Fernández, Juan Manuel
author2_role author
author
dc.subject.none.fl_str_mv Biología
osteoblast
bone marrow stem cells
bone microarchitecture
biomechanics
hops
phytoestrogens
topic Biología
osteoblast
bone marrow stem cells
bone microarchitecture
biomechanics
hops
phytoestrogens
dc.description.none.fl_txt_mv Osteoporosis is characterized by an imbalance between bone formation and resorption, leading to decreased bone mass and an increased fracture risk, mainly associated with aging. Current treatments include anti-resorptive and anabolic drugs. However, these often have side effects, leadingmany patients to seek natural biological alternatives. We have demonstrated previously that hops extract, rich in compounds with estrogenic activity classified as phytoestrogens, exerts osteogenic effects by promoting the osteoblastic differentiation of bone marrowstemcells (BMSCs)while inhibiting osteoclast activity in vitro. In our study, youngmale Sprague Dawley rats were randomized into two groups: one received hops extract (LPL, 1% w/v in drinking water) for two months, and the other drank water alone (C). The rats were euthanized, and their femurs were dissected and processed for static histomorphometry and bone biomechanics. Additionally, BMSCs were isolated from the humeri to evaluate their osteogenic potential. Our result demonstrated that LPL treatment enhanced the osteogenic potential of humeral BMSCs in ex vivo assays, upregulating osteogenic genes and downregulating proresorptivemarkers. These findings correlated with improved femoral bonemicroarchitecture and biomechanical parameters. In conclusion, a two-month treatment with LPL enhanced the osteogenic capacity of BMSCs, improving bonemicroarchitecture and biomechanical properties. These results suggest its potential as a natural alternative for promoting bone health.
Laboratorio de Investigación en Osteopatías y Metabolismo Mineral
description Osteoporosis is characterized by an imbalance between bone formation and resorption, leading to decreased bone mass and an increased fracture risk, mainly associated with aging. Current treatments include anti-resorptive and anabolic drugs. However, these often have side effects, leadingmany patients to seek natural biological alternatives. We have demonstrated previously that hops extract, rich in compounds with estrogenic activity classified as phytoestrogens, exerts osteogenic effects by promoting the osteoblastic differentiation of bone marrowstemcells (BMSCs)while inhibiting osteoclast activity in vitro. In our study, youngmale Sprague Dawley rats were randomized into two groups: one received hops extract (LPL, 1% w/v in drinking water) for two months, and the other drank water alone (C). The rats were euthanized, and their femurs were dissected and processed for static histomorphometry and bone biomechanics. Additionally, BMSCs were isolated from the humeri to evaluate their osteogenic potential. Our result demonstrated that LPL treatment enhanced the osteogenic potential of humeral BMSCs in ex vivo assays, upregulating osteogenic genes and downregulating proresorptivemarkers. These findings correlated with improved femoral bonemicroarchitecture and biomechanical parameters. In conclusion, a two-month treatment with LPL enhanced the osteogenic capacity of BMSCs, improving bonemicroarchitecture and biomechanical properties. These results suggest its potential as a natural alternative for promoting bone health.
publishDate 2025
dc.date.none.fl_str_mv 2025-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/181453
url http://sedici.unlp.edu.ar/handle/10915/181453
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2079-7737
info:eu-repo/semantics/altIdentifier/doi/10.3390/biology14050582
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 12.982451

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