Vaginal isolation of beta-haemolytic Streptococcus from bitches with and without neonatal deaths in the litters

Autores
Guerrero López, Ana Elizabeth; Stornelli, María Cecilia; Jurado, Susana Beatriz; Giacoboni, Gabriela Isabel; Sguazza, Guillermo Hernán; Sota, Rodolfo Luzbel de la; Stornelli, María Alejandra
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the study was to identify beta-haemolytic streptococci in the vagina of bitches who had delivered healthy litters and bitches who had delivered litters in which neonatal deaths occurred. Fifty-one bitches divided into two groups were used. Group 1 (G1) included 28 bitches that had delivered healthy litters and group 2 (G2) included 23 bitches that had delivered puppies who died in the neonatal period. Two vaginal samples were taken, one in proestrus and the other at the end of gestation (EG). Beta-haemolytic Streptococcus (BS) was isolated from 16 bitches (57%) in G1 and from 21 bitches (91%) in G2. The bacteriological cultures, serological tests (Streptex®) and PCR assay allowed identification of Streptococcus canis and Streptococcus dysgalactiae in G1 and G2. Ultramicroscopic studies allowed the observation of M Protein and capsules in strains of S. dysgalactiae and S. canis in G1 and G2. The S. canis strains isolated from G2 showed thicker capsules than S. canis strains isolated from G1 (234 ± 24.2 vs 151.23 ± 28.93 nm; p < .001.). No differences were observed in capsule thickness between strains of S. dysgalactiae isolated from G1 and G2 (210 ± 13.54 vs 211.66 ± 19.67 nm; p > .70). All strains of beta-haemolytic Streptococcus isolated were penicillin sensitive. Penicillin was administered from EG to 5 days post-partum in 10 G2 females with isolation of BS (G2A). Saline solution was administered in eleven G2 females with isolation of BS (G2B). Ninety per cent of the puppies survived in G2A and 25% survived in G2B. Our results suggest BS is involved in canine neonatal deaths.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
Bitch
Neonatal deaths
Streptococcus canis
Streptococcus dysgalactiae
Vagina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/104834
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/104834
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Vaginal isolation of beta-haemolytic Streptococcus from bitches with and without neonatal deaths in the littersGuerrero López, Ana ElizabethStornelli, María CeciliaJurado, Susana BeatrizGiacoboni, Gabriela IsabelSguazza, Guillermo HernánSota, Rodolfo Luzbel de laStornelli, María AlejandraCiencias VeterinariasBitchNeonatal deathsStreptococcus canisStreptococcus dysgalactiaeVaginaThe aim of the study was to identify beta-haemolytic streptococci in the vagina of bitches who had delivered healthy litters and bitches who had delivered litters in which neonatal deaths occurred. Fifty-one bitches divided into two groups were used. Group 1 (G1) included 28 bitches that had delivered healthy litters and group 2 (G2) included 23 bitches that had delivered puppies who died in the neonatal period. Two vaginal samples were taken, one in proestrus and the other at the end of gestation (EG). Beta-haemolytic Streptococcus (BS) was isolated from 16 bitches (57%) in G1 and from 21 bitches (91%) in G2. The bacteriological cultures, serological tests (Streptex®) and PCR assay allowed identification of Streptococcus canis and Streptococcus dysgalactiae in G1 and G2. Ultramicroscopic studies allowed the observation of M Protein and capsules in strains of S. dysgalactiae and S. canis in G1 and G2. The S. canis strains isolated from G2 showed thicker capsules than S. canis strains isolated from G1 (234 ± 24.2 vs 151.23 ± 28.93 nm; p &lt; .001.). No differences were observed in capsule thickness between strains of S. dysgalactiae isolated from G1 and G2 (210 ± 13.54 vs 211.66 ± 19.67 nm; p &gt; .70). All strains of beta-haemolytic Streptococcus isolated were penicillin sensitive. Penicillin was administered from EG to 5 days post-partum in 10 G2 females with isolation of BS (G2A). Saline solution was administered in eleven G2 females with isolation of BS (G2B). Ninety per cent of the puppies survived in G2A and 25% survived in G2B. Our results suggest BS is involved in canine neonatal deaths.Facultad de Ciencias Veterinarias2018-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf609-616http://sedici.unlp.edu.ar/handle/10915/104834enginfo:eu-repo/semantics/altIdentifier/issn/0936-6768info:eu-repo/semantics/altIdentifier/doi/10.1111/rda.13147info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:22:57Zoai:sedici.unlp.edu.ar:10915/104834Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:22:58.068SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Vaginal isolation of beta-haemolytic Streptococcus from bitches with and without neonatal deaths in the litters
title Vaginal isolation of beta-haemolytic Streptococcus from bitches with and without neonatal deaths in the litters
spellingShingle Vaginal isolation of beta-haemolytic Streptococcus from bitches with and without neonatal deaths in the litters
Guerrero López, Ana Elizabeth
Ciencias Veterinarias
Bitch
Neonatal deaths
Streptococcus canis
Streptococcus dysgalactiae
Vagina
title_short Vaginal isolation of beta-haemolytic Streptococcus from bitches with and without neonatal deaths in the litters
title_full Vaginal isolation of beta-haemolytic Streptococcus from bitches with and without neonatal deaths in the litters
title_fullStr Vaginal isolation of beta-haemolytic Streptococcus from bitches with and without neonatal deaths in the litters
title_full_unstemmed Vaginal isolation of beta-haemolytic Streptococcus from bitches with and without neonatal deaths in the litters
title_sort Vaginal isolation of beta-haemolytic Streptococcus from bitches with and without neonatal deaths in the litters
dc.creator.none.fl_str_mv Guerrero López, Ana Elizabeth
Stornelli, María Cecilia
Jurado, Susana Beatriz
Giacoboni, Gabriela Isabel
Sguazza, Guillermo Hernán
Sota, Rodolfo Luzbel de la
Stornelli, María Alejandra
author Guerrero López, Ana Elizabeth
author_facet Guerrero López, Ana Elizabeth
Stornelli, María Cecilia
Jurado, Susana Beatriz
Giacoboni, Gabriela Isabel
Sguazza, Guillermo Hernán
Sota, Rodolfo Luzbel de la
Stornelli, María Alejandra
author_role author
author2 Stornelli, María Cecilia
Jurado, Susana Beatriz
Giacoboni, Gabriela Isabel
Sguazza, Guillermo Hernán
Sota, Rodolfo Luzbel de la
Stornelli, María Alejandra
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Bitch
Neonatal deaths
Streptococcus canis
Streptococcus dysgalactiae
Vagina
topic Ciencias Veterinarias
Bitch
Neonatal deaths
Streptococcus canis
Streptococcus dysgalactiae
Vagina
dc.description.none.fl_txt_mv The aim of the study was to identify beta-haemolytic streptococci in the vagina of bitches who had delivered healthy litters and bitches who had delivered litters in which neonatal deaths occurred. Fifty-one bitches divided into two groups were used. Group 1 (G1) included 28 bitches that had delivered healthy litters and group 2 (G2) included 23 bitches that had delivered puppies who died in the neonatal period. Two vaginal samples were taken, one in proestrus and the other at the end of gestation (EG). Beta-haemolytic Streptococcus (BS) was isolated from 16 bitches (57%) in G1 and from 21 bitches (91%) in G2. The bacteriological cultures, serological tests (Streptex®) and PCR assay allowed identification of Streptococcus canis and Streptococcus dysgalactiae in G1 and G2. Ultramicroscopic studies allowed the observation of M Protein and capsules in strains of S. dysgalactiae and S. canis in G1 and G2. The S. canis strains isolated from G2 showed thicker capsules than S. canis strains isolated from G1 (234 ± 24.2 vs 151.23 ± 28.93 nm; p &lt; .001.). No differences were observed in capsule thickness between strains of S. dysgalactiae isolated from G1 and G2 (210 ± 13.54 vs 211.66 ± 19.67 nm; p &gt; .70). All strains of beta-haemolytic Streptococcus isolated were penicillin sensitive. Penicillin was administered from EG to 5 days post-partum in 10 G2 females with isolation of BS (G2A). Saline solution was administered in eleven G2 females with isolation of BS (G2B). Ninety per cent of the puppies survived in G2A and 25% survived in G2B. Our results suggest BS is involved in canine neonatal deaths.
Facultad de Ciencias Veterinarias
description The aim of the study was to identify beta-haemolytic streptococci in the vagina of bitches who had delivered healthy litters and bitches who had delivered litters in which neonatal deaths occurred. Fifty-one bitches divided into two groups were used. Group 1 (G1) included 28 bitches that had delivered healthy litters and group 2 (G2) included 23 bitches that had delivered puppies who died in the neonatal period. Two vaginal samples were taken, one in proestrus and the other at the end of gestation (EG). Beta-haemolytic Streptococcus (BS) was isolated from 16 bitches (57%) in G1 and from 21 bitches (91%) in G2. The bacteriological cultures, serological tests (Streptex®) and PCR assay allowed identification of Streptococcus canis and Streptococcus dysgalactiae in G1 and G2. Ultramicroscopic studies allowed the observation of M Protein and capsules in strains of S. dysgalactiae and S. canis in G1 and G2. The S. canis strains isolated from G2 showed thicker capsules than S. canis strains isolated from G1 (234 ± 24.2 vs 151.23 ± 28.93 nm; p &lt; .001.). No differences were observed in capsule thickness between strains of S. dysgalactiae isolated from G1 and G2 (210 ± 13.54 vs 211.66 ± 19.67 nm; p &gt; .70). All strains of beta-haemolytic Streptococcus isolated were penicillin sensitive. Penicillin was administered from EG to 5 days post-partum in 10 G2 females with isolation of BS (G2A). Saline solution was administered in eleven G2 females with isolation of BS (G2B). Ninety per cent of the puppies survived in G2A and 25% survived in G2B. Our results suggest BS is involved in canine neonatal deaths.
publishDate 2018
dc.date.none.fl_str_mv 2018-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/104834
url http://sedici.unlp.edu.ar/handle/10915/104834
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0936-6768
info:eu-repo/semantics/altIdentifier/doi/10.1111/rda.13147
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
609-616
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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