First time identification of <i>Pandoraea sputorum</i> from a patient with cystic fibrosis in Argentina: A case report

Autores
Martina, Pablo Francisco; Martínez, Mónica; Frada, Guillermo; Alvarez, Florencia; Leguizamón, Lorena; Prieto, Claudia Inés; Barrias, Carolina; Bettiol, Marisa; Lagares, Antonio; Bosch, María Alejandra; Ferreras, Julián; Von Specht, Martha
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Pandoraea species are considered emerging pathogens in the context of cystic fibrosis (CF) and are difficult to identify by conventional biochemical methods. These multidrug resistant bacteria remain poorly understood particularly in terms of natural resistance, mechanisms of acquired resistance and impact on the prognosis of the disease and the lung function. Among them, Pandoraea sputorum has been previously described in few cases of CF patients from Spain, Australia, France and United States, underlining the need of more clinical data for a better knowledge of its pathogenicity. This is the first report relating to P. sputorum in a CF patient in Argentina. Case presentation: Pandoraea sputorum was identified in a nine-year-old cystic fibrosis patient from Argentina, after treatment failure during an exacerbation. The isolates were successfully identified by combining molecular techniques based on 16S rRNA sequencing and mass spectrometry (MS) methods, after reassessing previous misidentified isolates by conventional methods. After first isolation of P. sputorum, patient's clinical condition worsened but later improved after a change in the treatment. Although isolates showed susceptibility to trimethoprim-sulfamethoxazole and imipenem, in our case, the antibiotic treatment failed in the eradication of P. sputorum. Conclusions: All combined data showed a chronic colonization with P. sputorum associated to a deterioration of lung function. We noted that the presence of P. sputorum can be underestimated in CF patients and MALDI-TOF MS appears to be a promising means of accurate identification of Pandoraea species.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Instituto de Biotecnologia y Biologia Molecular
Facultad de Ciencias Exactas
Materia
Ciencias Exactas
Argentina
Cystic fibrosis
First report
MALDI-TOF MS
Pandoraea sputorum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/87348

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spelling First time identification of <i>Pandoraea sputorum</i> from a patient with cystic fibrosis in Argentina: A case reportMartina, Pablo FranciscoMartínez, MónicaFrada, GuillermoAlvarez, FlorenciaLeguizamón, LorenaPrieto, Claudia InésBarrias, CarolinaBettiol, MarisaLagares, AntonioBosch, María AlejandraFerreras, JuliánVon Specht, MarthaCiencias ExactasArgentinaCystic fibrosisFirst reportMALDI-TOF MSPandoraea sputorumBackground: <i>Pandoraea</i> species are considered emerging pathogens in the context of cystic fibrosis (CF) and are difficult to identify by conventional biochemical methods. These multidrug resistant bacteria remain poorly understood particularly in terms of natural resistance, mechanisms of acquired resistance and impact on the prognosis of the disease and the lung function. Among them, <i>Pandoraea sputorum</i> has been previously described in few cases of CF patients from Spain, Australia, France and United States, underlining the need of more clinical data for a better knowledge of its pathogenicity. This is the first report relating to <i>P. sputorum</i> in a CF patient in Argentina. Case presentation: <i>Pandoraea sputorum</i> was identified in a nine-year-old cystic fibrosis patient from Argentina, after treatment failure during an exacerbation. The isolates were successfully identified by combining molecular techniques based on 16S rRNA sequencing and mass spectrometry (MS) methods, after reassessing previous misidentified isolates by conventional methods. After first isolation of <i>P. sputorum</i>, patient's clinical condition worsened but later improved after a change in the treatment. Although isolates showed susceptibility to trimethoprim-sulfamethoxazole and imipenem, in our case, the antibiotic treatment failed in the eradication of <i>P. sputorum</i>. Conclusions: All combined data showed a chronic colonization with <i>P. sputorum</i> associated to a deterioration of lung function. We noted that the presence of <i>P. sputorum</i> can be underestimated in CF patients and MALDI-TOF MS appears to be a promising means of accurate identification of <i>Pandoraea</i> species.Centro de Investigación y Desarrollo en Fermentaciones IndustrialesInstituto de Biotecnologia y Biologia MolecularFacultad de Ciencias Exactas2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/87348enginfo:eu-repo/semantics/altIdentifier/issn/1471-2466info:eu-repo/semantics/altIdentifier/doi/10.1186/s12890-017-0373-yinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:17:14Zoai:sedici.unlp.edu.ar:10915/87348Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:17:15.296SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv First time identification of <i>Pandoraea sputorum</i> from a patient with cystic fibrosis in Argentina: A case report
title First time identification of <i>Pandoraea sputorum</i> from a patient with cystic fibrosis in Argentina: A case report
spellingShingle First time identification of <i>Pandoraea sputorum</i> from a patient with cystic fibrosis in Argentina: A case report
Martina, Pablo Francisco
Ciencias Exactas
Argentina
Cystic fibrosis
First report
MALDI-TOF MS
Pandoraea sputorum
title_short First time identification of <i>Pandoraea sputorum</i> from a patient with cystic fibrosis in Argentina: A case report
title_full First time identification of <i>Pandoraea sputorum</i> from a patient with cystic fibrosis in Argentina: A case report
title_fullStr First time identification of <i>Pandoraea sputorum</i> from a patient with cystic fibrosis in Argentina: A case report
title_full_unstemmed First time identification of <i>Pandoraea sputorum</i> from a patient with cystic fibrosis in Argentina: A case report
title_sort First time identification of <i>Pandoraea sputorum</i> from a patient with cystic fibrosis in Argentina: A case report
dc.creator.none.fl_str_mv Martina, Pablo Francisco
Martínez, Mónica
Frada, Guillermo
Alvarez, Florencia
Leguizamón, Lorena
Prieto, Claudia Inés
Barrias, Carolina
Bettiol, Marisa
Lagares, Antonio
Bosch, María Alejandra
Ferreras, Julián
Von Specht, Martha
author Martina, Pablo Francisco
author_facet Martina, Pablo Francisco
Martínez, Mónica
Frada, Guillermo
Alvarez, Florencia
Leguizamón, Lorena
Prieto, Claudia Inés
Barrias, Carolina
Bettiol, Marisa
Lagares, Antonio
Bosch, María Alejandra
Ferreras, Julián
Von Specht, Martha
author_role author
author2 Martínez, Mónica
Frada, Guillermo
Alvarez, Florencia
Leguizamón, Lorena
Prieto, Claudia Inés
Barrias, Carolina
Bettiol, Marisa
Lagares, Antonio
Bosch, María Alejandra
Ferreras, Julián
Von Specht, Martha
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Argentina
Cystic fibrosis
First report
MALDI-TOF MS
Pandoraea sputorum
topic Ciencias Exactas
Argentina
Cystic fibrosis
First report
MALDI-TOF MS
Pandoraea sputorum
dc.description.none.fl_txt_mv Background: <i>Pandoraea</i> species are considered emerging pathogens in the context of cystic fibrosis (CF) and are difficult to identify by conventional biochemical methods. These multidrug resistant bacteria remain poorly understood particularly in terms of natural resistance, mechanisms of acquired resistance and impact on the prognosis of the disease and the lung function. Among them, <i>Pandoraea sputorum</i> has been previously described in few cases of CF patients from Spain, Australia, France and United States, underlining the need of more clinical data for a better knowledge of its pathogenicity. This is the first report relating to <i>P. sputorum</i> in a CF patient in Argentina. Case presentation: <i>Pandoraea sputorum</i> was identified in a nine-year-old cystic fibrosis patient from Argentina, after treatment failure during an exacerbation. The isolates were successfully identified by combining molecular techniques based on 16S rRNA sequencing and mass spectrometry (MS) methods, after reassessing previous misidentified isolates by conventional methods. After first isolation of <i>P. sputorum</i>, patient's clinical condition worsened but later improved after a change in the treatment. Although isolates showed susceptibility to trimethoprim-sulfamethoxazole and imipenem, in our case, the antibiotic treatment failed in the eradication of <i>P. sputorum</i>. Conclusions: All combined data showed a chronic colonization with <i>P. sputorum</i> associated to a deterioration of lung function. We noted that the presence of <i>P. sputorum</i> can be underestimated in CF patients and MALDI-TOF MS appears to be a promising means of accurate identification of <i>Pandoraea</i> species.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Instituto de Biotecnologia y Biologia Molecular
Facultad de Ciencias Exactas
description Background: <i>Pandoraea</i> species are considered emerging pathogens in the context of cystic fibrosis (CF) and are difficult to identify by conventional biochemical methods. These multidrug resistant bacteria remain poorly understood particularly in terms of natural resistance, mechanisms of acquired resistance and impact on the prognosis of the disease and the lung function. Among them, <i>Pandoraea sputorum</i> has been previously described in few cases of CF patients from Spain, Australia, France and United States, underlining the need of more clinical data for a better knowledge of its pathogenicity. This is the first report relating to <i>P. sputorum</i> in a CF patient in Argentina. Case presentation: <i>Pandoraea sputorum</i> was identified in a nine-year-old cystic fibrosis patient from Argentina, after treatment failure during an exacerbation. The isolates were successfully identified by combining molecular techniques based on 16S rRNA sequencing and mass spectrometry (MS) methods, after reassessing previous misidentified isolates by conventional methods. After first isolation of <i>P. sputorum</i>, patient's clinical condition worsened but later improved after a change in the treatment. Although isolates showed susceptibility to trimethoprim-sulfamethoxazole and imipenem, in our case, the antibiotic treatment failed in the eradication of <i>P. sputorum</i>. Conclusions: All combined data showed a chronic colonization with <i>P. sputorum</i> associated to a deterioration of lung function. We noted that the presence of <i>P. sputorum</i> can be underestimated in CF patients and MALDI-TOF MS appears to be a promising means of accurate identification of <i>Pandoraea</i> species.
publishDate 2017
dc.date.none.fl_str_mv 2017
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