First time identification of <i>Pandoraea sputorum</i> from a patient with cystic fibrosis in Argentina: A case report
- Autores
- Martina, Pablo Francisco; Martínez, Mónica; Frada, Guillermo; Alvarez, Florencia; Leguizamón, Lorena; Prieto, Claudia Inés; Barrias, Carolina; Bettiol, Marisa; Lagares, Antonio; Bosch, María Alejandra; Ferreras, Julián; Von Specht, Martha
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: Pandoraea species are considered emerging pathogens in the context of cystic fibrosis (CF) and are difficult to identify by conventional biochemical methods. These multidrug resistant bacteria remain poorly understood particularly in terms of natural resistance, mechanisms of acquired resistance and impact on the prognosis of the disease and the lung function. Among them, Pandoraea sputorum has been previously described in few cases of CF patients from Spain, Australia, France and United States, underlining the need of more clinical data for a better knowledge of its pathogenicity. This is the first report relating to P. sputorum in a CF patient in Argentina. Case presentation: Pandoraea sputorum was identified in a nine-year-old cystic fibrosis patient from Argentina, after treatment failure during an exacerbation. The isolates were successfully identified by combining molecular techniques based on 16S rRNA sequencing and mass spectrometry (MS) methods, after reassessing previous misidentified isolates by conventional methods. After first isolation of P. sputorum, patient's clinical condition worsened but later improved after a change in the treatment. Although isolates showed susceptibility to trimethoprim-sulfamethoxazole and imipenem, in our case, the antibiotic treatment failed in the eradication of P. sputorum. Conclusions: All combined data showed a chronic colonization with P. sputorum associated to a deterioration of lung function. We noted that the presence of P. sputorum can be underestimated in CF patients and MALDI-TOF MS appears to be a promising means of accurate identification of Pandoraea species.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Instituto de Biotecnologia y Biologia Molecular
Facultad de Ciencias Exactas - Materia
-
Ciencias Exactas
Argentina
Cystic fibrosis
First report
MALDI-TOF MS
Pandoraea sputorum - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/87348
Ver los metadatos del registro completo
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First time identification of <i>Pandoraea sputorum</i> from a patient with cystic fibrosis in Argentina: A case reportMartina, Pablo FranciscoMartínez, MónicaFrada, GuillermoAlvarez, FlorenciaLeguizamón, LorenaPrieto, Claudia InésBarrias, CarolinaBettiol, MarisaLagares, AntonioBosch, María AlejandraFerreras, JuliánVon Specht, MarthaCiencias ExactasArgentinaCystic fibrosisFirst reportMALDI-TOF MSPandoraea sputorumBackground: <i>Pandoraea</i> species are considered emerging pathogens in the context of cystic fibrosis (CF) and are difficult to identify by conventional biochemical methods. These multidrug resistant bacteria remain poorly understood particularly in terms of natural resistance, mechanisms of acquired resistance and impact on the prognosis of the disease and the lung function. Among them, <i>Pandoraea sputorum</i> has been previously described in few cases of CF patients from Spain, Australia, France and United States, underlining the need of more clinical data for a better knowledge of its pathogenicity. This is the first report relating to <i>P. sputorum</i> in a CF patient in Argentina. Case presentation: <i>Pandoraea sputorum</i> was identified in a nine-year-old cystic fibrosis patient from Argentina, after treatment failure during an exacerbation. The isolates were successfully identified by combining molecular techniques based on 16S rRNA sequencing and mass spectrometry (MS) methods, after reassessing previous misidentified isolates by conventional methods. After first isolation of <i>P. sputorum</i>, patient's clinical condition worsened but later improved after a change in the treatment. Although isolates showed susceptibility to trimethoprim-sulfamethoxazole and imipenem, in our case, the antibiotic treatment failed in the eradication of <i>P. sputorum</i>. Conclusions: All combined data showed a chronic colonization with <i>P. sputorum</i> associated to a deterioration of lung function. We noted that the presence of <i>P. sputorum</i> can be underestimated in CF patients and MALDI-TOF MS appears to be a promising means of accurate identification of <i>Pandoraea</i> species.Centro de Investigación y Desarrollo en Fermentaciones IndustrialesInstituto de Biotecnologia y Biologia MolecularFacultad de Ciencias Exactas2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/87348enginfo:eu-repo/semantics/altIdentifier/issn/1471-2466info:eu-repo/semantics/altIdentifier/doi/10.1186/s12890-017-0373-yinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:17:14Zoai:sedici.unlp.edu.ar:10915/87348Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:17:15.296SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
First time identification of <i>Pandoraea sputorum</i> from a patient with cystic fibrosis in Argentina: A case report |
title |
First time identification of <i>Pandoraea sputorum</i> from a patient with cystic fibrosis in Argentina: A case report |
spellingShingle |
First time identification of <i>Pandoraea sputorum</i> from a patient with cystic fibrosis in Argentina: A case report Martina, Pablo Francisco Ciencias Exactas Argentina Cystic fibrosis First report MALDI-TOF MS Pandoraea sputorum |
title_short |
First time identification of <i>Pandoraea sputorum</i> from a patient with cystic fibrosis in Argentina: A case report |
title_full |
First time identification of <i>Pandoraea sputorum</i> from a patient with cystic fibrosis in Argentina: A case report |
title_fullStr |
First time identification of <i>Pandoraea sputorum</i> from a patient with cystic fibrosis in Argentina: A case report |
title_full_unstemmed |
First time identification of <i>Pandoraea sputorum</i> from a patient with cystic fibrosis in Argentina: A case report |
title_sort |
First time identification of <i>Pandoraea sputorum</i> from a patient with cystic fibrosis in Argentina: A case report |
dc.creator.none.fl_str_mv |
Martina, Pablo Francisco Martínez, Mónica Frada, Guillermo Alvarez, Florencia Leguizamón, Lorena Prieto, Claudia Inés Barrias, Carolina Bettiol, Marisa Lagares, Antonio Bosch, María Alejandra Ferreras, Julián Von Specht, Martha |
author |
Martina, Pablo Francisco |
author_facet |
Martina, Pablo Francisco Martínez, Mónica Frada, Guillermo Alvarez, Florencia Leguizamón, Lorena Prieto, Claudia Inés Barrias, Carolina Bettiol, Marisa Lagares, Antonio Bosch, María Alejandra Ferreras, Julián Von Specht, Martha |
author_role |
author |
author2 |
Martínez, Mónica Frada, Guillermo Alvarez, Florencia Leguizamón, Lorena Prieto, Claudia Inés Barrias, Carolina Bettiol, Marisa Lagares, Antonio Bosch, María Alejandra Ferreras, Julián Von Specht, Martha |
author2_role |
author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Ciencias Exactas Argentina Cystic fibrosis First report MALDI-TOF MS Pandoraea sputorum |
topic |
Ciencias Exactas Argentina Cystic fibrosis First report MALDI-TOF MS Pandoraea sputorum |
dc.description.none.fl_txt_mv |
Background: <i>Pandoraea</i> species are considered emerging pathogens in the context of cystic fibrosis (CF) and are difficult to identify by conventional biochemical methods. These multidrug resistant bacteria remain poorly understood particularly in terms of natural resistance, mechanisms of acquired resistance and impact on the prognosis of the disease and the lung function. Among them, <i>Pandoraea sputorum</i> has been previously described in few cases of CF patients from Spain, Australia, France and United States, underlining the need of more clinical data for a better knowledge of its pathogenicity. This is the first report relating to <i>P. sputorum</i> in a CF patient in Argentina. Case presentation: <i>Pandoraea sputorum</i> was identified in a nine-year-old cystic fibrosis patient from Argentina, after treatment failure during an exacerbation. The isolates were successfully identified by combining molecular techniques based on 16S rRNA sequencing and mass spectrometry (MS) methods, after reassessing previous misidentified isolates by conventional methods. After first isolation of <i>P. sputorum</i>, patient's clinical condition worsened but later improved after a change in the treatment. Although isolates showed susceptibility to trimethoprim-sulfamethoxazole and imipenem, in our case, the antibiotic treatment failed in the eradication of <i>P. sputorum</i>. Conclusions: All combined data showed a chronic colonization with <i>P. sputorum</i> associated to a deterioration of lung function. We noted that the presence of <i>P. sputorum</i> can be underestimated in CF patients and MALDI-TOF MS appears to be a promising means of accurate identification of <i>Pandoraea</i> species. Centro de Investigación y Desarrollo en Fermentaciones Industriales Instituto de Biotecnologia y Biologia Molecular Facultad de Ciencias Exactas |
description |
Background: <i>Pandoraea</i> species are considered emerging pathogens in the context of cystic fibrosis (CF) and are difficult to identify by conventional biochemical methods. These multidrug resistant bacteria remain poorly understood particularly in terms of natural resistance, mechanisms of acquired resistance and impact on the prognosis of the disease and the lung function. Among them, <i>Pandoraea sputorum</i> has been previously described in few cases of CF patients from Spain, Australia, France and United States, underlining the need of more clinical data for a better knowledge of its pathogenicity. This is the first report relating to <i>P. sputorum</i> in a CF patient in Argentina. Case presentation: <i>Pandoraea sputorum</i> was identified in a nine-year-old cystic fibrosis patient from Argentina, after treatment failure during an exacerbation. The isolates were successfully identified by combining molecular techniques based on 16S rRNA sequencing and mass spectrometry (MS) methods, after reassessing previous misidentified isolates by conventional methods. After first isolation of <i>P. sputorum</i>, patient's clinical condition worsened but later improved after a change in the treatment. Although isolates showed susceptibility to trimethoprim-sulfamethoxazole and imipenem, in our case, the antibiotic treatment failed in the eradication of <i>P. sputorum</i>. Conclusions: All combined data showed a chronic colonization with <i>P. sputorum</i> associated to a deterioration of lung function. We noted that the presence of <i>P. sputorum</i> can be underestimated in CF patients and MALDI-TOF MS appears to be a promising means of accurate identification of <i>Pandoraea</i> species. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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