Equine Erythrocyte Lysed Exposed to T-Butyl Hydroperoxide as a Model to Study the Oxidative Stress Caused by Exercise Using a Chemiluminescence Assay

Autores
Savignone, César A.; Palacios, Alejandro
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present investigation was carried out to determine the presence of oxidative alterations in the horses erythrocyte membrane during a high intensity exercise test. The degree of peroxidation was estimated by chemiluminescence using a suspension of lysed erythrocytes incubated with t-butyl hydroperoxide (t-BHP). Differences were observed in the total values of chemiluminescence throughout the exercise routine, with higher values of light emission obtained with the animal at rest in relation to those observed during and after exercise.The conclusions of this study are the existence of changes in the erythrocyte membranes of the horses exposed to physical exertion, probably associated with the release of ROS caused by the exercise and that the determination of chemiluminescence in suspension of lysates erythrocyte is a sensitive assay applied to detect the existence of oxidative stress associated to physical exercise.
Facultad de Ciencias Veterinarias
Materia
Veterinaria
oxidative stress
exercise
chemiluminescence
erythrocyte
tert-butyl hydroperoxide
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc/3.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/78624
Acceder
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network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Equine Erythrocyte Lysed Exposed to T-Butyl Hydroperoxide as a Model to Study the Oxidative Stress Caused by Exercise Using a Chemiluminescence AssaySavignone, César A.Palacios, AlejandroVeterinariaoxidative stressexercisechemiluminescenceerythrocytetert-butyl hydroperoxideThe present investigation was carried out to determine the presence of oxidative alterations in the horses erythrocyte membrane during a high intensity exercise test. The degree of peroxidation was estimated by chemiluminescence using a suspension of lysed erythrocytes incubated with t-butyl hydroperoxide (t-BHP). Differences were observed in the total values of chemiluminescence throughout the exercise routine, with higher values of light emission obtained with the animal at rest in relation to those observed during and after exercise.The conclusions of this study are the existence of changes in the erythrocyte membranes of the horses exposed to physical exertion, probably associated with the release of ROS caused by the exercise and that the determination of chemiluminescence in suspension of lysates erythrocyte is a sensitive assay applied to detect the existence of oxidative stress associated to physical exercise.Facultad de Ciencias Veterinarias2017-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf19-24http://sedici.unlp.edu.ar/handle/10915/78624enginfo:eu-repo/semantics/altIdentifier/url/https://medicalresearchjournal.org/index.php/GJMR/article/view/1272info:eu-repo/semantics/altIdentifier/issn/2249-4618info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/3.0/Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:46:16Zoai:sedici.unlp.edu.ar:10915/78624Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:46:17.071SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Equine Erythrocyte Lysed Exposed to T-Butyl Hydroperoxide as a Model to Study the Oxidative Stress Caused by Exercise Using a Chemiluminescence Assay
title Equine Erythrocyte Lysed Exposed to T-Butyl Hydroperoxide as a Model to Study the Oxidative Stress Caused by Exercise Using a Chemiluminescence Assay
spellingShingle Equine Erythrocyte Lysed Exposed to T-Butyl Hydroperoxide as a Model to Study the Oxidative Stress Caused by Exercise Using a Chemiluminescence Assay
Savignone, César A.
Veterinaria
oxidative stress
exercise
chemiluminescence
erythrocyte
tert-butyl hydroperoxide
title_short Equine Erythrocyte Lysed Exposed to T-Butyl Hydroperoxide as a Model to Study the Oxidative Stress Caused by Exercise Using a Chemiluminescence Assay
title_full Equine Erythrocyte Lysed Exposed to T-Butyl Hydroperoxide as a Model to Study the Oxidative Stress Caused by Exercise Using a Chemiluminescence Assay
title_fullStr Equine Erythrocyte Lysed Exposed to T-Butyl Hydroperoxide as a Model to Study the Oxidative Stress Caused by Exercise Using a Chemiluminescence Assay
title_full_unstemmed Equine Erythrocyte Lysed Exposed to T-Butyl Hydroperoxide as a Model to Study the Oxidative Stress Caused by Exercise Using a Chemiluminescence Assay
title_sort Equine Erythrocyte Lysed Exposed to T-Butyl Hydroperoxide as a Model to Study the Oxidative Stress Caused by Exercise Using a Chemiluminescence Assay
dc.creator.none.fl_str_mv Savignone, César A.
Palacios, Alejandro
author Savignone, César A.
author_facet Savignone, César A.
Palacios, Alejandro
author_role author
author2 Palacios, Alejandro
author2_role author
dc.subject.none.fl_str_mv Veterinaria
oxidative stress
exercise
chemiluminescence
erythrocyte
tert-butyl hydroperoxide
topic Veterinaria
oxidative stress
exercise
chemiluminescence
erythrocyte
tert-butyl hydroperoxide
dc.description.none.fl_txt_mv The present investigation was carried out to determine the presence of oxidative alterations in the horses erythrocyte membrane during a high intensity exercise test. The degree of peroxidation was estimated by chemiluminescence using a suspension of lysed erythrocytes incubated with t-butyl hydroperoxide (t-BHP). Differences were observed in the total values of chemiluminescence throughout the exercise routine, with higher values of light emission obtained with the animal at rest in relation to those observed during and after exercise.The conclusions of this study are the existence of changes in the erythrocyte membranes of the horses exposed to physical exertion, probably associated with the release of ROS caused by the exercise and that the determination of chemiluminescence in suspension of lysates erythrocyte is a sensitive assay applied to detect the existence of oxidative stress associated to physical exercise.
Facultad de Ciencias Veterinarias
description The present investigation was carried out to determine the presence of oxidative alterations in the horses erythrocyte membrane during a high intensity exercise test. The degree of peroxidation was estimated by chemiluminescence using a suspension of lysed erythrocytes incubated with t-butyl hydroperoxide (t-BHP). Differences were observed in the total values of chemiluminescence throughout the exercise routine, with higher values of light emission obtained with the animal at rest in relation to those observed during and after exercise.The conclusions of this study are the existence of changes in the erythrocyte membranes of the horses exposed to physical exertion, probably associated with the release of ROS caused by the exercise and that the determination of chemiluminescence in suspension of lysates erythrocyte is a sensitive assay applied to detect the existence of oxidative stress associated to physical exercise.
publishDate 2017
dc.date.none.fl_str_mv 2017-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/78624
url http://sedici.unlp.edu.ar/handle/10915/78624
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medicalresearchjournal.org/index.php/GJMR/article/view/1272
info:eu-repo/semantics/altIdentifier/issn/2249-4618
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc/3.0/
Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/3.0/
Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)
dc.format.none.fl_str_mv application/pdf
19-24
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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