Impact of transient correction of increased adrenocortical activity in hypothalamo-damaged, hyperadipose female rats

Autores
Moreno, Griselda; Perelló, Mario; Camihort, Gisela; Luna, Georgina Cecilia; Cónsole-Avegliano, Gloria Miriam; Gaillard, R. C.; Spinedi, Eduardo
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: To explore the effects of transient correction of enhanced corticoadrenal activity in monosodium L-glutamate (MSG)-damaged female rats on peripheral insulin sensitivity and in vitro retroperitoneal (RP) adipocyte function. Designs: A dose of 4 mg/g body weight (BW) of MSG or vehicle (CTR) was i.p. injected, once every 2 days, between days 2 and 10 of age, in female rats. Intact and 21 day-operated (sham or adrenal enucleation (AE)) rats from both (CTR and MSG) groups were used for experimentation on day 120 of age. Circulating levels of several hormones, in basal and after i.v. high-glucose load conditions, and RP adiposity morphology and function were then evaluated. Results: MSG rats developed increased adrenocortical function, hyperadiposity, hyperleptinemia, hyperinsulinemia and decreased peripheral insulin sensitivity. These characteristics were fully reversed after transient correction of corticoadrenal hyperactivity induced by AE. In addition, in vitro experimentation with isolated RP adipocytes indicated that cells from intact MSG animals displayed decreased sensitivity to insulin and dexamethasone stimulation of leptin secretion. Interestingly, adipocyte dysfunction in MSG rats was fully abrogated after AE-induced transient correction of insulinemia, leptinemia and adrenocortical activity. Importantly, the reversion of these metabolic abnormalities, induced by AE for 21 days, in MSG animals did occur, despite no significant changes in BW values. Conclusion: Our results support that the changes in adipocyte characteristics and peripheral insulin resistance, developed in this pseudo-obese female rat model, are mainly due to increased glucocorticoid production. Importantly, appropriate correction of the enhanced adrenocortical activity fully reversed these abnormal functions.
Instituto Multidisciplinario de Biología Celular
Facultad de Ciencias Médicas
Facultad de Ciencias Exactas
Materia
Ciencias Médicas
Ciencias Exactas
ACTH
Fat morphometry
Glucocorticoid
Glucose
Insulin
Leptin
MSG
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/83294

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oai_identifier_str oai:sedici.unlp.edu.ar:10915/83294
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Impact of transient correction of increased adrenocortical activity in hypothalamo-damaged, hyperadipose female ratsMoreno, GriseldaPerelló, MarioCamihort, GiselaLuna, Georgina CeciliaCónsole-Avegliano, Gloria MiriamGaillard, R. C.Spinedi, EduardoCiencias MédicasCiencias ExactasACTHFat morphometryGlucocorticoidGlucoseInsulinLeptinMSGObjective: To explore the effects of transient correction of enhanced corticoadrenal activity in monosodium L-glutamate (MSG)-damaged female rats on peripheral insulin sensitivity and <i>in vitro</i> retroperitoneal (RP) adipocyte function. Designs: A dose of 4 mg/g body weight (BW) of MSG or vehicle (CTR) was i.p. injected, once every 2 days, between days 2 and 10 of age, in female rats. Intact and 21 day-operated (sham or adrenal enucleation (AE)) rats from both (CTR and MSG) groups were used for experimentation on day 120 of age. Circulating levels of several hormones, in basal and after i.v. high-glucose load conditions, and RP adiposity morphology and function were then evaluated. Results: MSG rats developed increased adrenocortical function, hyperadiposity, hyperleptinemia, hyperinsulinemia and decreased peripheral insulin sensitivity. These characteristics were fully reversed after transient correction of corticoadrenal hyperactivity induced by AE. In addition, <i>in vitro</i> experimentation with isolated RP adipocytes indicated that cells from intact MSG animals displayed decreased sensitivity to insulin and dexamethasone stimulation of leptin secretion. Interestingly, adipocyte dysfunction in MSG rats was fully abrogated after AE-induced transient correction of insulinemia, leptinemia and adrenocortical activity. Importantly, the reversion of these metabolic abnormalities, induced by AE for 21 days, in MSG animals did occur, despite no significant changes in BW values. Conclusion: Our results support that the changes in adipocyte characteristics and peripheral insulin resistance, developed in this pseudo-obese female rat model, are mainly due to increased glucocorticoid production. Importantly, appropriate correction of the enhanced adrenocortical activity fully reversed these abnormal functions.Instituto Multidisciplinario de Biología CelularFacultad de Ciencias MédicasFacultad de Ciencias Exactas2006info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf73-82http://sedici.unlp.edu.ar/handle/10915/83294enginfo:eu-repo/semantics/altIdentifier/issn/0307-0565info:eu-repo/semantics/altIdentifier/doi/10.1038/sj.ijo.0803109info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:15:46Zoai:sedici.unlp.edu.ar:10915/83294Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:15:46.804SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Impact of transient correction of increased adrenocortical activity in hypothalamo-damaged, hyperadipose female rats
title Impact of transient correction of increased adrenocortical activity in hypothalamo-damaged, hyperadipose female rats
spellingShingle Impact of transient correction of increased adrenocortical activity in hypothalamo-damaged, hyperadipose female rats
Moreno, Griselda
Ciencias Médicas
Ciencias Exactas
ACTH
Fat morphometry
Glucocorticoid
Glucose
Insulin
Leptin
MSG
title_short Impact of transient correction of increased adrenocortical activity in hypothalamo-damaged, hyperadipose female rats
title_full Impact of transient correction of increased adrenocortical activity in hypothalamo-damaged, hyperadipose female rats
title_fullStr Impact of transient correction of increased adrenocortical activity in hypothalamo-damaged, hyperadipose female rats
title_full_unstemmed Impact of transient correction of increased adrenocortical activity in hypothalamo-damaged, hyperadipose female rats
title_sort Impact of transient correction of increased adrenocortical activity in hypothalamo-damaged, hyperadipose female rats
dc.creator.none.fl_str_mv Moreno, Griselda
Perelló, Mario
Camihort, Gisela
Luna, Georgina Cecilia
Cónsole-Avegliano, Gloria Miriam
Gaillard, R. C.
Spinedi, Eduardo
author Moreno, Griselda
author_facet Moreno, Griselda
Perelló, Mario
Camihort, Gisela
Luna, Georgina Cecilia
Cónsole-Avegliano, Gloria Miriam
Gaillard, R. C.
Spinedi, Eduardo
author_role author
author2 Perelló, Mario
Camihort, Gisela
Luna, Georgina Cecilia
Cónsole-Avegliano, Gloria Miriam
Gaillard, R. C.
Spinedi, Eduardo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Médicas
Ciencias Exactas
ACTH
Fat morphometry
Glucocorticoid
Glucose
Insulin
Leptin
MSG
topic Ciencias Médicas
Ciencias Exactas
ACTH
Fat morphometry
Glucocorticoid
Glucose
Insulin
Leptin
MSG
dc.description.none.fl_txt_mv Objective: To explore the effects of transient correction of enhanced corticoadrenal activity in monosodium L-glutamate (MSG)-damaged female rats on peripheral insulin sensitivity and <i>in vitro</i> retroperitoneal (RP) adipocyte function. Designs: A dose of 4 mg/g body weight (BW) of MSG or vehicle (CTR) was i.p. injected, once every 2 days, between days 2 and 10 of age, in female rats. Intact and 21 day-operated (sham or adrenal enucleation (AE)) rats from both (CTR and MSG) groups were used for experimentation on day 120 of age. Circulating levels of several hormones, in basal and after i.v. high-glucose load conditions, and RP adiposity morphology and function were then evaluated. Results: MSG rats developed increased adrenocortical function, hyperadiposity, hyperleptinemia, hyperinsulinemia and decreased peripheral insulin sensitivity. These characteristics were fully reversed after transient correction of corticoadrenal hyperactivity induced by AE. In addition, <i>in vitro</i> experimentation with isolated RP adipocytes indicated that cells from intact MSG animals displayed decreased sensitivity to insulin and dexamethasone stimulation of leptin secretion. Interestingly, adipocyte dysfunction in MSG rats was fully abrogated after AE-induced transient correction of insulinemia, leptinemia and adrenocortical activity. Importantly, the reversion of these metabolic abnormalities, induced by AE for 21 days, in MSG animals did occur, despite no significant changes in BW values. Conclusion: Our results support that the changes in adipocyte characteristics and peripheral insulin resistance, developed in this pseudo-obese female rat model, are mainly due to increased glucocorticoid production. Importantly, appropriate correction of the enhanced adrenocortical activity fully reversed these abnormal functions.
Instituto Multidisciplinario de Biología Celular
Facultad de Ciencias Médicas
Facultad de Ciencias Exactas
description Objective: To explore the effects of transient correction of enhanced corticoadrenal activity in monosodium L-glutamate (MSG)-damaged female rats on peripheral insulin sensitivity and <i>in vitro</i> retroperitoneal (RP) adipocyte function. Designs: A dose of 4 mg/g body weight (BW) of MSG or vehicle (CTR) was i.p. injected, once every 2 days, between days 2 and 10 of age, in female rats. Intact and 21 day-operated (sham or adrenal enucleation (AE)) rats from both (CTR and MSG) groups were used for experimentation on day 120 of age. Circulating levels of several hormones, in basal and after i.v. high-glucose load conditions, and RP adiposity morphology and function were then evaluated. Results: MSG rats developed increased adrenocortical function, hyperadiposity, hyperleptinemia, hyperinsulinemia and decreased peripheral insulin sensitivity. These characteristics were fully reversed after transient correction of corticoadrenal hyperactivity induced by AE. In addition, <i>in vitro</i> experimentation with isolated RP adipocytes indicated that cells from intact MSG animals displayed decreased sensitivity to insulin and dexamethasone stimulation of leptin secretion. Interestingly, adipocyte dysfunction in MSG rats was fully abrogated after AE-induced transient correction of insulinemia, leptinemia and adrenocortical activity. Importantly, the reversion of these metabolic abnormalities, induced by AE for 21 days, in MSG animals did occur, despite no significant changes in BW values. Conclusion: Our results support that the changes in adipocyte characteristics and peripheral insulin resistance, developed in this pseudo-obese female rat model, are mainly due to increased glucocorticoid production. Importantly, appropriate correction of the enhanced adrenocortical activity fully reversed these abnormal functions.
publishDate 2006
dc.date.none.fl_str_mv 2006
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/83294
url http://sedici.unlp.edu.ar/handle/10915/83294
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0307-0565
info:eu-repo/semantics/altIdentifier/doi/10.1038/sj.ijo.0803109
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
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instname_str Universidad Nacional de La Plata
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institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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