Insulin-mimetic action of vanadium compounds on osteoblast-like cells in culture
- Autores
- Etcheverry, Susana B.; Crans, D. C.; Keramidas, A. D.; Cortizo, Ana María
- Año de publicación
- 1997
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Vanadium compounds mimic insulin actions in different cell types. The present study concerns the insulin-like effects of three vanadium(V) derivatives and one vanadium(IV) complex on osteoblast-like (UMR106 and MC3T3E1) cells in culture. The vanadium oxalate and vanadium citrate complexes hydrolyzed completely under the culture conditions, whereas more than 40% of the vanadium tartrate and nitrilotriacetate complexes remained. Vanadate, as well as vanadium oxalate, citrate, and tartrate complexes enhanced cell proliferation (as measured by the crystal violet assay), glucose consumption, and protein content in UMR106 and MC3T3E1 osteoblast-like cells. The vanadium nitrilotriacetate complex (the only peroxo complex tested) stimulated cell proliferation in UMR106 but not in MC3T3E1 cells. This derivative strongly transformed the morphology of the MC3T3E1 cells. All vanadium(V) compounds inhibited cell differentiation (alkaline phosphatase activity) in UMR106 cells. Our data are consistent with the interpretation that vanadium oxalate and citrate complexes hydrolyze to vanadate. Vanadium nitrilotriacetate would appear to be toxic for normal MC3T3E1 osteoblasts. In contrast, the vanadium tartrate complex induced a proliferative effect; however, it did not alter cell differentiation.
Facultad de Ciencias Exactas - Materia
-
Ciencias Exactas
vanadate, aqueous vanadium chemistry, vanastability, spectroscopy, proliferation, differentiation, glucose consumption, protein content, alkaline phosphatase, osteoblast-like cells
Química - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/76585
Ver los metadatos del registro completo
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Insulin-mimetic action of vanadium compounds on osteoblast-like cells in cultureEtcheverry, Susana B.Crans, D. C.Keramidas, A. D.Cortizo, Ana MaríaCiencias Exactasvanadate, aqueous vanadium chemistry, vanastability, spectroscopy, proliferation, differentiation, glucose consumption, protein content, alkaline phosphatase, osteoblast-like cellsQuímicaVanadium compounds mimic insulin actions in different cell types. The present study concerns the insulin-like effects of three vanadium(V) derivatives and one vanadium(IV) complex on osteoblast-like (UMR106 and MC3T3E1) cells in culture. The vanadium oxalate and vanadium citrate complexes hydrolyzed completely under the culture conditions, whereas more than 40% of the vanadium tartrate and nitrilotriacetate complexes remained. Vanadate, as well as vanadium oxalate, citrate, and tartrate complexes enhanced cell proliferation (as measured by the crystal violet assay), glucose consumption, and protein content in UMR106 and MC3T3E1 osteoblast-like cells. The vanadium nitrilotriacetate complex (the only peroxo complex tested) stimulated cell proliferation in UMR106 but not in MC3T3E1 cells. This derivative strongly transformed the morphology of the MC3T3E1 cells. All vanadium(V) compounds inhibited cell differentiation (alkaline phosphatase activity) in UMR106 cells. Our data are consistent with the interpretation that vanadium oxalate and citrate complexes hydrolyze to vanadate. Vanadium nitrilotriacetate would appear to be toxic for normal MC3T3E1 osteoblasts. In contrast, the vanadium tartrate complex induced a proliferative effect; however, it did not alter cell differentiation.Facultad de Ciencias Exactas1997info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf7-14http://sedici.unlp.edu.ar/handle/10915/76585enginfo:eu-repo/semantics/altIdentifier/hdl/11746/4779info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:45:35Zoai:sedici.unlp.edu.ar:10915/76585Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:45:35.866SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Insulin-mimetic action of vanadium compounds on osteoblast-like cells in culture |
title |
Insulin-mimetic action of vanadium compounds on osteoblast-like cells in culture |
spellingShingle |
Insulin-mimetic action of vanadium compounds on osteoblast-like cells in culture Etcheverry, Susana B. Ciencias Exactas vanadate, aqueous vanadium chemistry, vanastability, spectroscopy, proliferation, differentiation, glucose consumption, protein content, alkaline phosphatase, osteoblast-like cells Química |
title_short |
Insulin-mimetic action of vanadium compounds on osteoblast-like cells in culture |
title_full |
Insulin-mimetic action of vanadium compounds on osteoblast-like cells in culture |
title_fullStr |
Insulin-mimetic action of vanadium compounds on osteoblast-like cells in culture |
title_full_unstemmed |
Insulin-mimetic action of vanadium compounds on osteoblast-like cells in culture |
title_sort |
Insulin-mimetic action of vanadium compounds on osteoblast-like cells in culture |
dc.creator.none.fl_str_mv |
Etcheverry, Susana B. Crans, D. C. Keramidas, A. D. Cortizo, Ana María |
author |
Etcheverry, Susana B. |
author_facet |
Etcheverry, Susana B. Crans, D. C. Keramidas, A. D. Cortizo, Ana María |
author_role |
author |
author2 |
Crans, D. C. Keramidas, A. D. Cortizo, Ana María |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Ciencias Exactas vanadate, aqueous vanadium chemistry, vanastability, spectroscopy, proliferation, differentiation, glucose consumption, protein content, alkaline phosphatase, osteoblast-like cells Química |
topic |
Ciencias Exactas vanadate, aqueous vanadium chemistry, vanastability, spectroscopy, proliferation, differentiation, glucose consumption, protein content, alkaline phosphatase, osteoblast-like cells Química |
dc.description.none.fl_txt_mv |
Vanadium compounds mimic insulin actions in different cell types. The present study concerns the insulin-like effects of three vanadium(V) derivatives and one vanadium(IV) complex on osteoblast-like (UMR106 and MC3T3E1) cells in culture. The vanadium oxalate and vanadium citrate complexes hydrolyzed completely under the culture conditions, whereas more than 40% of the vanadium tartrate and nitrilotriacetate complexes remained. Vanadate, as well as vanadium oxalate, citrate, and tartrate complexes enhanced cell proliferation (as measured by the crystal violet assay), glucose consumption, and protein content in UMR106 and MC3T3E1 osteoblast-like cells. The vanadium nitrilotriacetate complex (the only peroxo complex tested) stimulated cell proliferation in UMR106 but not in MC3T3E1 cells. This derivative strongly transformed the morphology of the MC3T3E1 cells. All vanadium(V) compounds inhibited cell differentiation (alkaline phosphatase activity) in UMR106 cells. Our data are consistent with the interpretation that vanadium oxalate and citrate complexes hydrolyze to vanadate. Vanadium nitrilotriacetate would appear to be toxic for normal MC3T3E1 osteoblasts. In contrast, the vanadium tartrate complex induced a proliferative effect; however, it did not alter cell differentiation. Facultad de Ciencias Exactas |
description |
Vanadium compounds mimic insulin actions in different cell types. The present study concerns the insulin-like effects of three vanadium(V) derivatives and one vanadium(IV) complex on osteoblast-like (UMR106 and MC3T3E1) cells in culture. The vanadium oxalate and vanadium citrate complexes hydrolyzed completely under the culture conditions, whereas more than 40% of the vanadium tartrate and nitrilotriacetate complexes remained. Vanadate, as well as vanadium oxalate, citrate, and tartrate complexes enhanced cell proliferation (as measured by the crystal violet assay), glucose consumption, and protein content in UMR106 and MC3T3E1 osteoblast-like cells. The vanadium nitrilotriacetate complex (the only peroxo complex tested) stimulated cell proliferation in UMR106 but not in MC3T3E1 cells. This derivative strongly transformed the morphology of the MC3T3E1 cells. All vanadium(V) compounds inhibited cell differentiation (alkaline phosphatase activity) in UMR106 cells. Our data are consistent with the interpretation that vanadium oxalate and citrate complexes hydrolyze to vanadate. Vanadium nitrilotriacetate would appear to be toxic for normal MC3T3E1 osteoblasts. In contrast, the vanadium tartrate complex induced a proliferative effect; however, it did not alter cell differentiation. |
publishDate |
1997 |
dc.date.none.fl_str_mv |
1997 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/76585 |
url |
http://sedici.unlp.edu.ar/handle/10915/76585 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/hdl/11746/4779 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
dc.format.none.fl_str_mv |
application/pdf 7-14 |
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