A recombinant iron transport protein from Bordetella pertussis confers protection against Bordetella parapertussis

Autores
Álvarez Hayes, Jimena; Oviedo, Juan Marcos; Valdez, Hugo Alberto; Laborde, Juan Martín; Maschi, Fabricio Alejandro; Ayala, Miguel Ángel; Shah, Rohan; Fernandez Lahore, Marcelo; Rodríguez, María Eugenia
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Whooping cough, which is caused by Bordetella pertussis and B. parapertussis, is a reemerging disease. New protective antigens are needed to improve the efficacy of current vaccines against both species. Using proteomic tools, it was here found that B. parapertussis expresses a homolog of AfuA, a previously reported new vaccine candidate against B. pertussis. It was found that this homolog, named AfuABpp, is expressed during B. parapertussis infection, exposed on the surface of the bacteria and recognized by specific antibodies induced by the recombinant AfuA cloned from B. pertussis (rAfuA). Importantly, the presence of the O-antigen, a molecule that has been found to shield surface antigens on B. parapertussis, showed no influence on antibody recognition of AfuABpp on the bacterial surface. The present study further showed that antibodies induced by immunization with the recombinant protein were able to opsonize B. parapertussis and promote bacterial uptake by neutrophils. Finally, it was shown that this antigen confers protection against B. parapertussis infection in a mouse model. Altogether, these results indicate that AfuA is a good vaccine candidate for acellular vaccines protective against both causative agents of whooping cough.
Facultad de Ciencias Exactas
Facultad de Ciencias Veterinarias (FCV)
Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI)
Materia
Química
Bordetella parapertussis
new antigens
vaccine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/79424
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/79424
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling A recombinant iron transport protein from Bordetella pertussis confers protection against Bordetella parapertussisÁlvarez Hayes, JimenaOviedo, Juan MarcosValdez, Hugo AlbertoLaborde, Juan MartínMaschi, Fabricio AlejandroAyala, Miguel ÁngelShah, RohanFernandez Lahore, MarceloRodríguez, María EugeniaQuímicaBordetella parapertussisnew antigensvaccineWhooping cough, which is caused by Bordetella pertussis and B. parapertussis, is a reemerging disease. New protective antigens are needed to improve the efficacy of current vaccines against both species. Using proteomic tools, it was here found that B. parapertussis expresses a homolog of AfuA, a previously reported new vaccine candidate against B. pertussis. It was found that this homolog, named AfuABpp, is expressed during B. parapertussis infection, exposed on the surface of the bacteria and recognized by specific antibodies induced by the recombinant AfuA cloned from B. pertussis (rAfuA). Importantly, the presence of the O-antigen, a molecule that has been found to shield surface antigens on B. parapertussis, showed no influence on antibody recognition of AfuABpp on the bacterial surface. The present study further showed that antibodies induced by immunization with the recombinant protein were able to opsonize B. parapertussis and promote bacterial uptake by neutrophils. Finally, it was shown that this antigen confers protection against B. parapertussis infection in a mouse model. Altogether, these results indicate that AfuA is a good vaccine candidate for acellular vaccines protective against both causative agents of whooping cough.Facultad de Ciencias ExactasFacultad de Ciencias Veterinarias (FCV)Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI)2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf407-415http://sedici.unlp.edu.ar/handle/10915/79424enginfo:eu-repo/semantics/altIdentifier/doi/10.1111/1348-0421.12532info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:06:24Zoai:sedici.unlp.edu.ar:10915/79424Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:06:24.425SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv A recombinant iron transport protein from Bordetella pertussis confers protection against Bordetella parapertussis
title A recombinant iron transport protein from Bordetella pertussis confers protection against Bordetella parapertussis
spellingShingle A recombinant iron transport protein from Bordetella pertussis confers protection against Bordetella parapertussis
Álvarez Hayes, Jimena
Química
Bordetella parapertussis
new antigens
vaccine
title_short A recombinant iron transport protein from Bordetella pertussis confers protection against Bordetella parapertussis
title_full A recombinant iron transport protein from Bordetella pertussis confers protection against Bordetella parapertussis
title_fullStr A recombinant iron transport protein from Bordetella pertussis confers protection against Bordetella parapertussis
title_full_unstemmed A recombinant iron transport protein from Bordetella pertussis confers protection against Bordetella parapertussis
title_sort A recombinant iron transport protein from Bordetella pertussis confers protection against Bordetella parapertussis
dc.creator.none.fl_str_mv Álvarez Hayes, Jimena
Oviedo, Juan Marcos
Valdez, Hugo Alberto
Laborde, Juan Martín
Maschi, Fabricio Alejandro
Ayala, Miguel Ángel
Shah, Rohan
Fernandez Lahore, Marcelo
Rodríguez, María Eugenia
author Álvarez Hayes, Jimena
author_facet Álvarez Hayes, Jimena
Oviedo, Juan Marcos
Valdez, Hugo Alberto
Laborde, Juan Martín
Maschi, Fabricio Alejandro
Ayala, Miguel Ángel
Shah, Rohan
Fernandez Lahore, Marcelo
Rodríguez, María Eugenia
author_role author
author2 Oviedo, Juan Marcos
Valdez, Hugo Alberto
Laborde, Juan Martín
Maschi, Fabricio Alejandro
Ayala, Miguel Ángel
Shah, Rohan
Fernandez Lahore, Marcelo
Rodríguez, María Eugenia
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Química
Bordetella parapertussis
new antigens
vaccine
topic Química
Bordetella parapertussis
new antigens
vaccine
dc.description.none.fl_txt_mv Whooping cough, which is caused by Bordetella pertussis and B. parapertussis, is a reemerging disease. New protective antigens are needed to improve the efficacy of current vaccines against both species. Using proteomic tools, it was here found that B. parapertussis expresses a homolog of AfuA, a previously reported new vaccine candidate against B. pertussis. It was found that this homolog, named AfuABpp, is expressed during B. parapertussis infection, exposed on the surface of the bacteria and recognized by specific antibodies induced by the recombinant AfuA cloned from B. pertussis (rAfuA). Importantly, the presence of the O-antigen, a molecule that has been found to shield surface antigens on B. parapertussis, showed no influence on antibody recognition of AfuABpp on the bacterial surface. The present study further showed that antibodies induced by immunization with the recombinant protein were able to opsonize B. parapertussis and promote bacterial uptake by neutrophils. Finally, it was shown that this antigen confers protection against B. parapertussis infection in a mouse model. Altogether, these results indicate that AfuA is a good vaccine candidate for acellular vaccines protective against both causative agents of whooping cough.
Facultad de Ciencias Exactas
Facultad de Ciencias Veterinarias (FCV)
Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI)
description Whooping cough, which is caused by Bordetella pertussis and B. parapertussis, is a reemerging disease. New protective antigens are needed to improve the efficacy of current vaccines against both species. Using proteomic tools, it was here found that B. parapertussis expresses a homolog of AfuA, a previously reported new vaccine candidate against B. pertussis. It was found that this homolog, named AfuABpp, is expressed during B. parapertussis infection, exposed on the surface of the bacteria and recognized by specific antibodies induced by the recombinant AfuA cloned from B. pertussis (rAfuA). Importantly, the presence of the O-antigen, a molecule that has been found to shield surface antigens on B. parapertussis, showed no influence on antibody recognition of AfuABpp on the bacterial surface. The present study further showed that antibodies induced by immunization with the recombinant protein were able to opsonize B. parapertussis and promote bacterial uptake by neutrophils. Finally, it was shown that this antigen confers protection against B. parapertussis infection in a mouse model. Altogether, these results indicate that AfuA is a good vaccine candidate for acellular vaccines protective against both causative agents of whooping cough.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/79424
url http://sedici.unlp.edu.ar/handle/10915/79424
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/1348-0421.12532
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
407-415
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.22299

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