Purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineering

Autores
Torres, María Luz; Fernández, Juan Manuel; Dellatorre, Fernando Gaspar; Cortizo, Ana María; Oberti, Tamara Gisela
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
There is a growing interest about using natural polymers from renewable sources as biomaterials for applications in tissue engineering. In the present work, alginates were extracted from "Undaria pinnatifida", a brown sea weed invasive in Argentinian coast. The isolated alginate was structurally characterized by IR and ¹H NMR spectroscopies, intrinsic viscosity and TGA. For comparison purposes, commercial sodium alginate was purified and characterized using the same protocol as for raw material. Toxicity and biocompatibility of sodium alginate obtained from algae were studied using a murine macrophage-like cell line (RAW 264.7) and bone marrow stromal cells (BMSC), respectively. The presence of impurities inhibited both RAW 264.7 and bone marrow stromal cell proliferation and increased nitric oxide production from macrophages, while inhibited osteoblastic differentiation of BMSC. All these effects were reverted by the purification of alginate. In conclusion, alginate purification improves biocompatibility and osteo-induction while decreases its toxicity.
Laboratorio de Investigación en Osteopatías y Metabolismo Mineral
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Materia
Química
Biología
sodium alginate
structural characterization
purification
biocompatibility
cytotoxicity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/163984
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/163984
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineeringTorres, María LuzFernández, Juan ManuelDellatorre, Fernando GasparCortizo, Ana MaríaOberti, Tamara GiselaQuímicaBiologíasodium alginatestructural characterizationpurificationbiocompatibilitycytotoxicityThere is a growing interest about using natural polymers from renewable sources as biomaterials for applications in tissue engineering. In the present work, alginates were extracted from "Undaria pinnatifida", a brown sea weed invasive in Argentinian coast. The isolated alginate was structurally characterized by IR and ¹H NMR spectroscopies, intrinsic viscosity and TGA. For comparison purposes, commercial sodium alginate was purified and characterized using the same protocol as for raw material. Toxicity and biocompatibility of sodium alginate obtained from algae were studied using a murine macrophage-like cell line (RAW 264.7) and bone marrow stromal cells (BMSC), respectively. The presence of impurities inhibited both RAW 264.7 and bone marrow stromal cell proliferation and increased nitric oxide production from macrophages, while inhibited osteoblastic differentiation of BMSC. All these effects were reverted by the purification of alginate. In conclusion, alginate purification improves biocompatibility and osteo-induction while decreases its toxicity.Laboratorio de Investigación en Osteopatías y Metabolismo MineralInstituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas2019-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/163984enginfo:eu-repo/semantics/altIdentifier/issn/2211-9264info:eu-repo/semantics/altIdentifier/doi/10.1016/j.algal.2019.101499info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:34:57Zoai:sedici.unlp.edu.ar:10915/163984Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:34:57.849SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineering
title Purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineering
spellingShingle Purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineering
Torres, María Luz
Química
Biología
sodium alginate
structural characterization
purification
biocompatibility
cytotoxicity
title_short Purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineering
title_full Purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineering
title_fullStr Purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineering
title_full_unstemmed Purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineering
title_sort Purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineering
dc.creator.none.fl_str_mv Torres, María Luz
Fernández, Juan Manuel
Dellatorre, Fernando Gaspar
Cortizo, Ana María
Oberti, Tamara Gisela
author Torres, María Luz
author_facet Torres, María Luz
Fernández, Juan Manuel
Dellatorre, Fernando Gaspar
Cortizo, Ana María
Oberti, Tamara Gisela
author_role author
author2 Fernández, Juan Manuel
Dellatorre, Fernando Gaspar
Cortizo, Ana María
Oberti, Tamara Gisela
author2_role author
author
author
author
dc.subject.none.fl_str_mv Química
Biología
sodium alginate
structural characterization
purification
biocompatibility
cytotoxicity
topic Química
Biología
sodium alginate
structural characterization
purification
biocompatibility
cytotoxicity
dc.description.none.fl_txt_mv There is a growing interest about using natural polymers from renewable sources as biomaterials for applications in tissue engineering. In the present work, alginates were extracted from "Undaria pinnatifida", a brown sea weed invasive in Argentinian coast. The isolated alginate was structurally characterized by IR and ¹H NMR spectroscopies, intrinsic viscosity and TGA. For comparison purposes, commercial sodium alginate was purified and characterized using the same protocol as for raw material. Toxicity and biocompatibility of sodium alginate obtained from algae were studied using a murine macrophage-like cell line (RAW 264.7) and bone marrow stromal cells (BMSC), respectively. The presence of impurities inhibited both RAW 264.7 and bone marrow stromal cell proliferation and increased nitric oxide production from macrophages, while inhibited osteoblastic differentiation of BMSC. All these effects were reverted by the purification of alginate. In conclusion, alginate purification improves biocompatibility and osteo-induction while decreases its toxicity.
Laboratorio de Investigación en Osteopatías y Metabolismo Mineral
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
description There is a growing interest about using natural polymers from renewable sources as biomaterials for applications in tissue engineering. In the present work, alginates were extracted from "Undaria pinnatifida", a brown sea weed invasive in Argentinian coast. The isolated alginate was structurally characterized by IR and ¹H NMR spectroscopies, intrinsic viscosity and TGA. For comparison purposes, commercial sodium alginate was purified and characterized using the same protocol as for raw material. Toxicity and biocompatibility of sodium alginate obtained from algae were studied using a murine macrophage-like cell line (RAW 264.7) and bone marrow stromal cells (BMSC), respectively. The presence of impurities inhibited both RAW 264.7 and bone marrow stromal cell proliferation and increased nitric oxide production from macrophages, while inhibited osteoblastic differentiation of BMSC. All these effects were reverted by the purification of alginate. In conclusion, alginate purification improves biocompatibility and osteo-induction while decreases its toxicity.
publishDate 2019
dc.date.none.fl_str_mv 2019-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/163984
url http://sedici.unlp.edu.ar/handle/10915/163984
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2211-9264
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.algal.2019.101499
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.22299

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