Detection of β-Lactamase-Producing <i>Enterococcus faecalis</i> and Vancomycin-Resistant <i>Enterococcus faecium</i> Isolates in Human Invasive Infections in the Public Hospital of...

Autores
Schell, Celia María Beatriz; Tedim, Ana P.; Rodríguez Baños, Mercedes; Sparo, Mónica Delfina; Lissarrague, Sabina; Basualdo Farjat, Juan Ángel; Coque, Teresa M.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The study’s aim was to analyze the population structure of enterococci causing human invasive infections in a medium-sized Argentinian Hospital coincidental with a 5 year-period of increased recovery of antibiotic resistant enterococci (2010–2014). Species identification (biochemical testing/MALDI-TOF-MS), antimicrobial susceptibility (disk-diffusion) and clonal relatedness (PFGE/MLST/BAPS) were determined according to standard guidelines. β-lactamase production was determined by a nitrocefin test and confirmed by PCR/sequencing. The isolates were identified as Enterococcus faecalis and Enterococcus faecium at a 2:1 ratio. Most of the E. faecalis isolates, grouped in 25 PFGE-types (ST9/ST179/ST236/ST281/ST388/ST604/ST720), were resistant to high-levels (HLR) of gentamicin/streptomycin. A ST9 clone (bla+/HLR-gentamicin) was detected in patients of different wards during 2014. E. faecium isolates were grouped in 10 PFGE-types (ST25/ST18/ST19/ST52/ST792), with a low rate of ampicillin resistance. Five vancomycin-resistant E. faecium, three vanA (ST792/ST25) and two vanB (ST25) were detected. The ST25 clone carried either vanA or vanB. The recovery of a bla+-ST9-E. faecalis clone similar to that described in the late 1980s in Argentina suggests the possibility of a local hidden reservoir. These results reflect the relevance of local epidemiology in understanding the population structure of enterococci as well as the emergence and spread of antimicrobial resistance in predominant enterococcal clonal lineages.
Comisión de Investigaciones Científicas de la provincia de Buenos Aires
Centro Universitario de Estudios Microbiológicos y Parasitológicos
Materia
Ciencias Médicas
Enterococcus faecalis
Enterococcus faecium
invasive infections
antibiotic resistance
VRE
bla+
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/107346
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/107346
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Detection of β-Lactamase-Producing <i>Enterococcus faecalis</i> and Vancomycin-Resistant <i>Enterococcus faecium</i> Isolates in Human Invasive Infections in the Public Hospital of Tandil, ArgentinaSchell, Celia María BeatrizTedim, Ana P.Rodríguez Baños, MercedesSparo, Mónica DelfinaLissarrague, SabinaBasualdo Farjat, Juan ÁngelCoque, Teresa M.Ciencias MédicasEnterococcus faecalisEnterococcus faeciuminvasive infectionsantibiotic resistanceVREbla+The study’s aim was to analyze the population structure of enterococci causing human invasive infections in a medium-sized Argentinian Hospital coincidental with a 5 year-period of increased recovery of antibiotic resistant enterococci (2010–2014). Species identification (biochemical testing/MALDI-TOF-MS), antimicrobial susceptibility (disk-diffusion) and clonal relatedness (PFGE/MLST/BAPS) were determined according to standard guidelines. β-lactamase production was determined by a nitrocefin test and confirmed by PCR/sequencing. The isolates were identified as Enterococcus faecalis and Enterococcus faecium at a 2:1 ratio. Most of the E. faecalis isolates, grouped in 25 PFGE-types (ST9/ST179/ST236/ST281/ST388/ST604/ST720), were resistant to high-levels (HLR) of gentamicin/streptomycin. A ST9 clone (bla+/HLR-gentamicin) was detected in patients of different wards during 2014. E. faecium isolates were grouped in 10 PFGE-types (ST25/ST18/ST19/ST52/ST792), with a low rate of ampicillin resistance. Five vancomycin-resistant E. faecium, three vanA (ST792/ST25) and two vanB (ST25) were detected. The ST25 clone carried either vanA or vanB. The recovery of a bla+-ST9-E. faecalis clone similar to that described in the late 1980s in Argentina suggests the possibility of a local hidden reservoir. These results reflect the relevance of local epidemiology in understanding the population structure of enterococci as well as the emergence and spread of antimicrobial resistance in predominant enterococcal clonal lineages.Comisión de Investigaciones Científicas de la provincia de Buenos AiresCentro Universitario de Estudios Microbiológicos y Parasitológicos2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/107346enginfo:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7168638&blobtype=pdfinfo:eu-repo/semantics/altIdentifier/issn/2076-0817info:eu-repo/semantics/altIdentifier/pmid/32093230info:eu-repo/semantics/altIdentifier/doi/10.3390/pathogens9020142info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:56:06Zoai:sedici.unlp.edu.ar:10915/107346Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:56:06.856SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Detection of β-Lactamase-Producing <i>Enterococcus faecalis</i> and Vancomycin-Resistant <i>Enterococcus faecium</i> Isolates in Human Invasive Infections in the Public Hospital of Tandil, Argentina
title Detection of β-Lactamase-Producing <i>Enterococcus faecalis</i> and Vancomycin-Resistant <i>Enterococcus faecium</i> Isolates in Human Invasive Infections in the Public Hospital of Tandil, Argentina
spellingShingle Detection of β-Lactamase-Producing <i>Enterococcus faecalis</i> and Vancomycin-Resistant <i>Enterococcus faecium</i> Isolates in Human Invasive Infections in the Public Hospital of Tandil, Argentina
Schell, Celia María Beatriz
Ciencias Médicas
Enterococcus faecalis
Enterococcus faecium
invasive infections
antibiotic resistance
VRE
bla+
title_short Detection of β-Lactamase-Producing <i>Enterococcus faecalis</i> and Vancomycin-Resistant <i>Enterococcus faecium</i> Isolates in Human Invasive Infections in the Public Hospital of Tandil, Argentina
title_full Detection of β-Lactamase-Producing <i>Enterococcus faecalis</i> and Vancomycin-Resistant <i>Enterococcus faecium</i> Isolates in Human Invasive Infections in the Public Hospital of Tandil, Argentina
title_fullStr Detection of β-Lactamase-Producing <i>Enterococcus faecalis</i> and Vancomycin-Resistant <i>Enterococcus faecium</i> Isolates in Human Invasive Infections in the Public Hospital of Tandil, Argentina
title_full_unstemmed Detection of β-Lactamase-Producing <i>Enterococcus faecalis</i> and Vancomycin-Resistant <i>Enterococcus faecium</i> Isolates in Human Invasive Infections in the Public Hospital of Tandil, Argentina
title_sort Detection of β-Lactamase-Producing <i>Enterococcus faecalis</i> and Vancomycin-Resistant <i>Enterococcus faecium</i> Isolates in Human Invasive Infections in the Public Hospital of Tandil, Argentina
dc.creator.none.fl_str_mv Schell, Celia María Beatriz
Tedim, Ana P.
Rodríguez Baños, Mercedes
Sparo, Mónica Delfina
Lissarrague, Sabina
Basualdo Farjat, Juan Ángel
Coque, Teresa M.
author Schell, Celia María Beatriz
author_facet Schell, Celia María Beatriz
Tedim, Ana P.
Rodríguez Baños, Mercedes
Sparo, Mónica Delfina
Lissarrague, Sabina
Basualdo Farjat, Juan Ángel
Coque, Teresa M.
author_role author
author2 Tedim, Ana P.
Rodríguez Baños, Mercedes
Sparo, Mónica Delfina
Lissarrague, Sabina
Basualdo Farjat, Juan Ángel
Coque, Teresa M.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Médicas
Enterococcus faecalis
Enterococcus faecium
invasive infections
antibiotic resistance
VRE
bla+
topic Ciencias Médicas
Enterococcus faecalis
Enterococcus faecium
invasive infections
antibiotic resistance
VRE
bla+
dc.description.none.fl_txt_mv The study’s aim was to analyze the population structure of enterococci causing human invasive infections in a medium-sized Argentinian Hospital coincidental with a 5 year-period of increased recovery of antibiotic resistant enterococci (2010–2014). Species identification (biochemical testing/MALDI-TOF-MS), antimicrobial susceptibility (disk-diffusion) and clonal relatedness (PFGE/MLST/BAPS) were determined according to standard guidelines. β-lactamase production was determined by a nitrocefin test and confirmed by PCR/sequencing. The isolates were identified as Enterococcus faecalis and Enterococcus faecium at a 2:1 ratio. Most of the E. faecalis isolates, grouped in 25 PFGE-types (ST9/ST179/ST236/ST281/ST388/ST604/ST720), were resistant to high-levels (HLR) of gentamicin/streptomycin. A ST9 clone (bla+/HLR-gentamicin) was detected in patients of different wards during 2014. E. faecium isolates were grouped in 10 PFGE-types (ST25/ST18/ST19/ST52/ST792), with a low rate of ampicillin resistance. Five vancomycin-resistant E. faecium, three vanA (ST792/ST25) and two vanB (ST25) were detected. The ST25 clone carried either vanA or vanB. The recovery of a bla+-ST9-E. faecalis clone similar to that described in the late 1980s in Argentina suggests the possibility of a local hidden reservoir. These results reflect the relevance of local epidemiology in understanding the population structure of enterococci as well as the emergence and spread of antimicrobial resistance in predominant enterococcal clonal lineages.
Comisión de Investigaciones Científicas de la provincia de Buenos Aires
Centro Universitario de Estudios Microbiológicos y Parasitológicos
description The study’s aim was to analyze the population structure of enterococci causing human invasive infections in a medium-sized Argentinian Hospital coincidental with a 5 year-period of increased recovery of antibiotic resistant enterococci (2010–2014). Species identification (biochemical testing/MALDI-TOF-MS), antimicrobial susceptibility (disk-diffusion) and clonal relatedness (PFGE/MLST/BAPS) were determined according to standard guidelines. β-lactamase production was determined by a nitrocefin test and confirmed by PCR/sequencing. The isolates were identified as Enterococcus faecalis and Enterococcus faecium at a 2:1 ratio. Most of the E. faecalis isolates, grouped in 25 PFGE-types (ST9/ST179/ST236/ST281/ST388/ST604/ST720), were resistant to high-levels (HLR) of gentamicin/streptomycin. A ST9 clone (bla+/HLR-gentamicin) was detected in patients of different wards during 2014. E. faecium isolates were grouped in 10 PFGE-types (ST25/ST18/ST19/ST52/ST792), with a low rate of ampicillin resistance. Five vancomycin-resistant E. faecium, three vanA (ST792/ST25) and two vanB (ST25) were detected. The ST25 clone carried either vanA or vanB. The recovery of a bla+-ST9-E. faecalis clone similar to that described in the late 1980s in Argentina suggests the possibility of a local hidden reservoir. These results reflect the relevance of local epidemiology in understanding the population structure of enterococci as well as the emergence and spread of antimicrobial resistance in predominant enterococcal clonal lineages.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/107346
url http://sedici.unlp.edu.ar/handle/10915/107346
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/issn/2076-0817
info:eu-repo/semantics/altIdentifier/pmid/32093230
info:eu-repo/semantics/altIdentifier/doi/10.3390/pathogens9020142
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
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reponame_str SEDICI (UNLP)
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instname_str Universidad Nacional de La Plata
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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