Antarctic yeasts: potential use in a biologic treatment of textile azo dyes

Autores
Ruscasso, María Florencia; Cavello, Ivana Alejandra; Curutchet, Gustavo Andrés; Cavalitto, Sebastián Fernando
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We investigated the dye-removal potential of a collection of 61 cold-adapted yeasts from the King George Island, Antarctica, on agar plates supplemented with 100 mg L⁻¹ of several textile dyes; among which isolates 81% decolorized Reactive Black 5 (RB-5), with 56% decolorizing Reactive Orange 16, but only 26% doing so with Reactive Blue 19 and Acid Blue 74. Furthermore, we evaluated the ligninolytic potential using 2,2ʹ-azino-bis(3-ethylbenzothiazoline-6- sulfonic-acid) diammonium salt-, 3,5-dimethoxy-4-hydroxybenzaldehydazine-, or manganese-supplemented plates but detected no activity, possibly due to a dye-removal mechanism involving reductases. The removal kinetics were studied in liquid medium supplemented with 100 mg L⁻¹ of RB-5 in a selection of 9 yeasts. The highest volumetricremoval rates (η) were found for Candida sake 41E (4.14 mg L⁻¹ h⁻¹), Leucosporidium muscorum F20A (3.90 mg L⁻¹ h⁻¹), and Cystofilobasidium infirmominiatum F13E (3.90 mg L⁻¹ h⁻¹). Different UV–Vis spectra were obtained if the dye removal occurred by biodegradation or biosorption/bioaccumulation. L. muscorum F20A was selected to study the dye-removal mechanism of RB-5 and the effect of different chemical and environmental parameters on the process. Optimum dye-removal conditions were obtained with 10 g L⁻¹ of glucose within an initial medium pH range of 5.0 to 6.0. Up to 700 mg ⁻¹ of dye could be removed in 45 h. High-performance liquid chromatography profiles obtained were consistent with a biodegradation of the dye. Phytotoxicity was estimated by calculating the 50%-inhibition concentration ( IC50) with Lactuca sativa L. seeds. These findings propose psychrophilic yeasts as a novel environmentally suitable alternative for the treatment of dye-industry wastewaters.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Bioquímica
Leucosporidium muscorum F20A
Cold-adapted yeasts
Textile wastewaters
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/154820

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spelling Antarctic yeasts: potential use in a biologic treatment of textile azo dyesRuscasso, María FlorenciaCavello, Ivana AlejandraCurutchet, Gustavo AndrésCavalitto, Sebastián FernandoBioquímicaLeucosporidium muscorum F20ACold-adapted yeastsTextile wastewatersWe investigated the dye-removal potential of a collection of 61 cold-adapted yeasts from the King George Island, Antarctica, on agar plates supplemented with 100 mg L⁻¹ of several textile dyes; among which isolates 81% decolorized Reactive Black 5 (RB-5), with 56% decolorizing Reactive Orange 16, but only 26% doing so with Reactive Blue 19 and Acid Blue 74. Furthermore, we evaluated the ligninolytic potential using 2,2ʹ-azino-bis(3-ethylbenzothiazoline-6- sulfonic-acid) diammonium salt-, 3,5-dimethoxy-4-hydroxybenzaldehydazine-, or manganese-supplemented plates but detected no activity, possibly due to a dye-removal mechanism involving reductases. The removal kinetics were studied in liquid medium supplemented with 100 mg L⁻¹ of RB-5 in a selection of 9 yeasts. The highest volumetricremoval rates (η) were found for Candida sake 41E (4.14 mg L⁻¹ h⁻¹), Leucosporidium muscorum F20A (3.90 mg L⁻¹ h⁻¹), and Cystofilobasidium infirmominiatum F13E (3.90 mg L⁻¹ h⁻¹). Different UV–Vis spectra were obtained if the dye removal occurred by biodegradation or biosorption/bioaccumulation. L. muscorum F20A was selected to study the dye-removal mechanism of RB-5 and the effect of different chemical and environmental parameters on the process. Optimum dye-removal conditions were obtained with 10 g L⁻¹ of glucose within an initial medium pH range of 5.0 to 6.0. Up to 700 mg ⁻¹ of dye could be removed in 45 h. High-performance liquid chromatography profiles obtained were consistent with a biodegradation of the dye. Phytotoxicity was estimated by calculating the 50%-inhibition concentration ( IC50) with Lactuca sativa L. seeds. These findings propose psychrophilic yeasts as a novel environmentally suitable alternative for the treatment of dye-industry wastewaters.Centro de Investigación y Desarrollo en Fermentaciones Industriales2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/154820enginfo:eu-repo/semantics/altIdentifier/issn/2197-4365info:eu-repo/semantics/altIdentifier/doi/10.1186/s40643-022-00507-5info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:40:15Zoai:sedici.unlp.edu.ar:10915/154820Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:40:15.507SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Antarctic yeasts: potential use in a biologic treatment of textile azo dyes
title Antarctic yeasts: potential use in a biologic treatment of textile azo dyes
spellingShingle Antarctic yeasts: potential use in a biologic treatment of textile azo dyes
Ruscasso, María Florencia
Bioquímica
Leucosporidium muscorum F20A
Cold-adapted yeasts
Textile wastewaters
title_short Antarctic yeasts: potential use in a biologic treatment of textile azo dyes
title_full Antarctic yeasts: potential use in a biologic treatment of textile azo dyes
title_fullStr Antarctic yeasts: potential use in a biologic treatment of textile azo dyes
title_full_unstemmed Antarctic yeasts: potential use in a biologic treatment of textile azo dyes
title_sort Antarctic yeasts: potential use in a biologic treatment of textile azo dyes
dc.creator.none.fl_str_mv Ruscasso, María Florencia
Cavello, Ivana Alejandra
Curutchet, Gustavo Andrés
Cavalitto, Sebastián Fernando
author Ruscasso, María Florencia
author_facet Ruscasso, María Florencia
Cavello, Ivana Alejandra
Curutchet, Gustavo Andrés
Cavalitto, Sebastián Fernando
author_role author
author2 Cavello, Ivana Alejandra
Curutchet, Gustavo Andrés
Cavalitto, Sebastián Fernando
author2_role author
author
author
dc.subject.none.fl_str_mv Bioquímica
Leucosporidium muscorum F20A
Cold-adapted yeasts
Textile wastewaters
topic Bioquímica
Leucosporidium muscorum F20A
Cold-adapted yeasts
Textile wastewaters
dc.description.none.fl_txt_mv We investigated the dye-removal potential of a collection of 61 cold-adapted yeasts from the King George Island, Antarctica, on agar plates supplemented with 100 mg L⁻¹ of several textile dyes; among which isolates 81% decolorized Reactive Black 5 (RB-5), with 56% decolorizing Reactive Orange 16, but only 26% doing so with Reactive Blue 19 and Acid Blue 74. Furthermore, we evaluated the ligninolytic potential using 2,2ʹ-azino-bis(3-ethylbenzothiazoline-6- sulfonic-acid) diammonium salt-, 3,5-dimethoxy-4-hydroxybenzaldehydazine-, or manganese-supplemented plates but detected no activity, possibly due to a dye-removal mechanism involving reductases. The removal kinetics were studied in liquid medium supplemented with 100 mg L⁻¹ of RB-5 in a selection of 9 yeasts. The highest volumetricremoval rates (η) were found for Candida sake 41E (4.14 mg L⁻¹ h⁻¹), Leucosporidium muscorum F20A (3.90 mg L⁻¹ h⁻¹), and Cystofilobasidium infirmominiatum F13E (3.90 mg L⁻¹ h⁻¹). Different UV–Vis spectra were obtained if the dye removal occurred by biodegradation or biosorption/bioaccumulation. L. muscorum F20A was selected to study the dye-removal mechanism of RB-5 and the effect of different chemical and environmental parameters on the process. Optimum dye-removal conditions were obtained with 10 g L⁻¹ of glucose within an initial medium pH range of 5.0 to 6.0. Up to 700 mg ⁻¹ of dye could be removed in 45 h. High-performance liquid chromatography profiles obtained were consistent with a biodegradation of the dye. Phytotoxicity was estimated by calculating the 50%-inhibition concentration ( IC50) with Lactuca sativa L. seeds. These findings propose psychrophilic yeasts as a novel environmentally suitable alternative for the treatment of dye-industry wastewaters.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description We investigated the dye-removal potential of a collection of 61 cold-adapted yeasts from the King George Island, Antarctica, on agar plates supplemented with 100 mg L⁻¹ of several textile dyes; among which isolates 81% decolorized Reactive Black 5 (RB-5), with 56% decolorizing Reactive Orange 16, but only 26% doing so with Reactive Blue 19 and Acid Blue 74. Furthermore, we evaluated the ligninolytic potential using 2,2ʹ-azino-bis(3-ethylbenzothiazoline-6- sulfonic-acid) diammonium salt-, 3,5-dimethoxy-4-hydroxybenzaldehydazine-, or manganese-supplemented plates but detected no activity, possibly due to a dye-removal mechanism involving reductases. The removal kinetics were studied in liquid medium supplemented with 100 mg L⁻¹ of RB-5 in a selection of 9 yeasts. The highest volumetricremoval rates (η) were found for Candida sake 41E (4.14 mg L⁻¹ h⁻¹), Leucosporidium muscorum F20A (3.90 mg L⁻¹ h⁻¹), and Cystofilobasidium infirmominiatum F13E (3.90 mg L⁻¹ h⁻¹). Different UV–Vis spectra were obtained if the dye removal occurred by biodegradation or biosorption/bioaccumulation. L. muscorum F20A was selected to study the dye-removal mechanism of RB-5 and the effect of different chemical and environmental parameters on the process. Optimum dye-removal conditions were obtained with 10 g L⁻¹ of glucose within an initial medium pH range of 5.0 to 6.0. Up to 700 mg ⁻¹ of dye could be removed in 45 h. High-performance liquid chromatography profiles obtained were consistent with a biodegradation of the dye. Phytotoxicity was estimated by calculating the 50%-inhibition concentration ( IC50) with Lactuca sativa L. seeds. These findings propose psychrophilic yeasts as a novel environmentally suitable alternative for the treatment of dye-industry wastewaters.
publishDate 2022
dc.date.none.fl_str_mv 2022
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Creative Commons Attribution 4.0 International (CC BY 4.0)
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