Nature of changes in adrenocortical function in chronic hyperleptinemic female rats

Autores
Perelló, Mario Carlos; Moreno, Griselda Noemí; Camihort, Gisela Amelia; Luna, Georgina Cecilia; Console-Avegliano, Gloria Miriam; Gaillard, Rolf C.; Spinedi, Eduardo Julio
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neonatal treatment of rats with monosodium l-glutamate, which destroys hypothalamic arcuate nucleus neuronal bodies, induces several metabolic abnormalities; as a result, rats develop a phenotype of pseudoobesity. This study was designed to explore, in the monosodium l-glutamate-treated female rat, the influence of chronic hyperleptinemia on adrenal cortex functionality. For this purpose, we evaluated in control and hypothalamic-damaged rats: (a) in vivo and in vitro adrenocortical function, (b) adrenal leptin receptor immunodistribution and mRNA expression, and (c) whether the inhibitory effect of leptin on adrenal function remains. Our results indicate that, compared to normal counterparts, pseudoobese animals displayed (1) hyperadiposity, despite being hypophagic and of lower body weight, (2) in vivo and in vitro enhanced adrenocortical response to ACTH stimulation, (3) an in vitro adrenal fasciculata-reticularis cell hyper-sensitivity to ACTH stimulus, (4) hyperplasia of their adrenal zona fasciculata cells, and (5) adrenal fasciculata-reticularis cell refractoriness to the inhibitory effect of leptin on ACTH-stimulated glucocorticoid production due, at least in part, to decreased adrenal leptin receptor expression. These data further support that increased hypothalamo-pituitary-adrenal axis function, in the adult neurotoxin-lesioned female rat, is mainly dependent on the development of both hyperplasia of adrenal zona fasciculata and adrenal gland refractoriness to leptin inhibitory effect. Our study supports that adrenal leptin resistance could be responsible, at least in part, for enhanced glucocorticoid circulating levels in this phenotype of obesity.
Instituto Multidisciplinario de Biología Celular
Materia
Bioquímica
:ARC damage
Hypothalamic obesity
Positive energetic balance
Ob-Rb
Adiposity
Adrenal gland
Glucocorticoid
Leptin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/138342
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/138342
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Nature of changes in adrenocortical function in chronic hyperleptinemic female ratsPerelló, Mario CarlosMoreno, Griselda NoemíCamihort, Gisela AmeliaLuna, Georgina CeciliaConsole-Avegliano, Gloria MiriamGaillard, Rolf C.Spinedi, Eduardo JulioBioquímica:ARC damageHypothalamic obesityPositive energetic balanceOb-RbAdiposityAdrenal glandGlucocorticoidLeptinNeonatal treatment of rats with monosodium l-glutamate, which destroys hypothalamic arcuate nucleus neuronal bodies, induces several metabolic abnormalities; as a result, rats develop a phenotype of pseudoobesity. This study was designed to explore, in the monosodium l-glutamate-treated female rat, the influence of chronic hyperleptinemia on adrenal cortex functionality. For this purpose, we evaluated in control and hypothalamic-damaged rats: (a) in vivo and in vitro adrenocortical function, (b) adrenal leptin receptor immunodistribution and mRNA expression, and (c) whether the inhibitory effect of leptin on adrenal function remains. Our results indicate that, compared to normal counterparts, pseudoobese animals displayed (1) hyperadiposity, despite being hypophagic and of lower body weight, (2) in vivo and in vitro enhanced adrenocortical response to ACTH stimulation, (3) an in vitro adrenal fasciculata-reticularis cell hyper-sensitivity to ACTH stimulus, (4) hyperplasia of their adrenal zona fasciculata cells, and (5) adrenal fasciculata-reticularis cell refractoriness to the inhibitory effect of leptin on ACTH-stimulated glucocorticoid production due, at least in part, to decreased adrenal leptin receptor expression. These data further support that increased hypothalamo-pituitary-adrenal axis function, in the adult neurotoxin-lesioned female rat, is mainly dependent on the development of both hyperplasia of adrenal zona fasciculata and adrenal gland refractoriness to leptin inhibitory effect. Our study supports that adrenal leptin resistance could be responsible, at least in part, for enhanced glucocorticoid circulating levels in this phenotype of obesity.Instituto Multidisciplinario de Biología Celular2004info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf167-175http://sedici.unlp.edu.ar/handle/10915/138342enginfo:eu-repo/semantics/altIdentifier/issn/1355-008Xinfo:eu-repo/semantics/altIdentifier/issn/0969-711Xinfo:eu-repo/semantics/altIdentifier/doi/10.1385/endo:24:2:167info:eu-repo/semantics/altIdentifier/pmid/15347844info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:26Zoai:sedici.unlp.edu.ar:10915/138342Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:27.12SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Nature of changes in adrenocortical function in chronic hyperleptinemic female rats
title Nature of changes in adrenocortical function in chronic hyperleptinemic female rats
spellingShingle Nature of changes in adrenocortical function in chronic hyperleptinemic female rats
Perelló, Mario Carlos
Bioquímica
:ARC damage
Hypothalamic obesity
Positive energetic balance
Ob-Rb
Adiposity
Adrenal gland
Glucocorticoid
Leptin
title_short Nature of changes in adrenocortical function in chronic hyperleptinemic female rats
title_full Nature of changes in adrenocortical function in chronic hyperleptinemic female rats
title_fullStr Nature of changes in adrenocortical function in chronic hyperleptinemic female rats
title_full_unstemmed Nature of changes in adrenocortical function in chronic hyperleptinemic female rats
title_sort Nature of changes in adrenocortical function in chronic hyperleptinemic female rats
dc.creator.none.fl_str_mv Perelló, Mario Carlos
Moreno, Griselda Noemí
Camihort, Gisela Amelia
Luna, Georgina Cecilia
Console-Avegliano, Gloria Miriam
Gaillard, Rolf C.
Spinedi, Eduardo Julio
author Perelló, Mario Carlos
author_facet Perelló, Mario Carlos
Moreno, Griselda Noemí
Camihort, Gisela Amelia
Luna, Georgina Cecilia
Console-Avegliano, Gloria Miriam
Gaillard, Rolf C.
Spinedi, Eduardo Julio
author_role author
author2 Moreno, Griselda Noemí
Camihort, Gisela Amelia
Luna, Georgina Cecilia
Console-Avegliano, Gloria Miriam
Gaillard, Rolf C.
Spinedi, Eduardo Julio
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Bioquímica
:ARC damage
Hypothalamic obesity
Positive energetic balance
Ob-Rb
Adiposity
Adrenal gland
Glucocorticoid
Leptin
topic Bioquímica
:ARC damage
Hypothalamic obesity
Positive energetic balance
Ob-Rb
Adiposity
Adrenal gland
Glucocorticoid
Leptin
dc.description.none.fl_txt_mv Neonatal treatment of rats with monosodium l-glutamate, which destroys hypothalamic arcuate nucleus neuronal bodies, induces several metabolic abnormalities; as a result, rats develop a phenotype of pseudoobesity. This study was designed to explore, in the monosodium l-glutamate-treated female rat, the influence of chronic hyperleptinemia on adrenal cortex functionality. For this purpose, we evaluated in control and hypothalamic-damaged rats: (a) in vivo and in vitro adrenocortical function, (b) adrenal leptin receptor immunodistribution and mRNA expression, and (c) whether the inhibitory effect of leptin on adrenal function remains. Our results indicate that, compared to normal counterparts, pseudoobese animals displayed (1) hyperadiposity, despite being hypophagic and of lower body weight, (2) in vivo and in vitro enhanced adrenocortical response to ACTH stimulation, (3) an in vitro adrenal fasciculata-reticularis cell hyper-sensitivity to ACTH stimulus, (4) hyperplasia of their adrenal zona fasciculata cells, and (5) adrenal fasciculata-reticularis cell refractoriness to the inhibitory effect of leptin on ACTH-stimulated glucocorticoid production due, at least in part, to decreased adrenal leptin receptor expression. These data further support that increased hypothalamo-pituitary-adrenal axis function, in the adult neurotoxin-lesioned female rat, is mainly dependent on the development of both hyperplasia of adrenal zona fasciculata and adrenal gland refractoriness to leptin inhibitory effect. Our study supports that adrenal leptin resistance could be responsible, at least in part, for enhanced glucocorticoid circulating levels in this phenotype of obesity.
Instituto Multidisciplinario de Biología Celular
description Neonatal treatment of rats with monosodium l-glutamate, which destroys hypothalamic arcuate nucleus neuronal bodies, induces several metabolic abnormalities; as a result, rats develop a phenotype of pseudoobesity. This study was designed to explore, in the monosodium l-glutamate-treated female rat, the influence of chronic hyperleptinemia on adrenal cortex functionality. For this purpose, we evaluated in control and hypothalamic-damaged rats: (a) in vivo and in vitro adrenocortical function, (b) adrenal leptin receptor immunodistribution and mRNA expression, and (c) whether the inhibitory effect of leptin on adrenal function remains. Our results indicate that, compared to normal counterparts, pseudoobese animals displayed (1) hyperadiposity, despite being hypophagic and of lower body weight, (2) in vivo and in vitro enhanced adrenocortical response to ACTH stimulation, (3) an in vitro adrenal fasciculata-reticularis cell hyper-sensitivity to ACTH stimulus, (4) hyperplasia of their adrenal zona fasciculata cells, and (5) adrenal fasciculata-reticularis cell refractoriness to the inhibitory effect of leptin on ACTH-stimulated glucocorticoid production due, at least in part, to decreased adrenal leptin receptor expression. These data further support that increased hypothalamo-pituitary-adrenal axis function, in the adult neurotoxin-lesioned female rat, is mainly dependent on the development of both hyperplasia of adrenal zona fasciculata and adrenal gland refractoriness to leptin inhibitory effect. Our study supports that adrenal leptin resistance could be responsible, at least in part, for enhanced glucocorticoid circulating levels in this phenotype of obesity.
publishDate 2004
dc.date.none.fl_str_mv 2004
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/138342
url http://sedici.unlp.edu.ar/handle/10915/138342
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1355-008X
info:eu-repo/semantics/altIdentifier/issn/0969-711X
info:eu-repo/semantics/altIdentifier/doi/10.1385/endo:24:2:167
info:eu-repo/semantics/altIdentifier/pmid/15347844
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
167-175
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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