Screening for a culture medium yielding optimal colony growth, zoospore yield and infectivity of different isolates of Leptolegnia chapmanii (Straminipila: Peronosporomycetes)

Autores
Pelizza, Sebastián Alberto; Cabello, Marta Noemí; Tranchida, María Cecilia; Scorsetti, Ana Clara; Bisaro, Vilma
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Leptolegnia chapmanii is an aquatic fungus that has demonstrated marked pathogenicity towards the larvae of a number of mosquito species with little or no effect on non-target insects. The aim of this work was to determine the best culture medium for colony growth, and production and infectivity of zoospores among different isolates of L. chapmanii. No significant differences (P >0.01) were observed for the media FORT(agar), PYG, YPSS, and PYGSF in terms of colony diameter of the different isolates evaluated. In these culture media, all L. chapmanii isolates had the highest growth (45 mm2) after 7 days. The nine isolates tested produced the greatest number of zoospores in FORT(agar) medium 3 (1 × 106 zoospores/ml). Medium YPSS exhibited significant differences (P <0.0001) compared to other culture media with respect to maintenance of virulence and infectivity of the isolates, producing 100% larval mortality after 48 h of inoculation. Thus, for the cultivation and maintenance of L. chapmanii isolates, the medium of choice among those tested is FORT(agar), as it is both inexpensive to prepare and ensures optimal colony development, extremely high zoospore production, and excellent maintenance of fungal infectivity.
Centro de Estudios Parasitológicos y de Vectores
Materia
Ciencias Naturales
Ciencias Agrarias
Leptolegnia chapmanii
Isolate
Culture medium
Aedes aegypti
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/131877
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/131877
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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Screening for a culture medium yielding optimal colony growth, zoospore yield and infectivity of different isolates of Leptolegnia chapmanii (Straminipila: Peronosporomycetes)Pelizza, Sebastián AlbertoCabello, Marta NoemíTranchida, María CeciliaScorsetti, Ana ClaraBisaro, VilmaCiencias NaturalesCiencias AgrariasLeptolegnia chapmaniiIsolateCulture mediumAedes aegyptiLeptolegnia chapmanii is an aquatic fungus that has demonstrated marked pathogenicity towards the larvae of a number of mosquito species with little or no effect on non-target insects. The aim of this work was to determine the best culture medium for colony growth, and production and infectivity of zoospores among different isolates of L. chapmanii. No significant differences (P >0.01) were observed for the media FORT(agar), PYG, YPSS, and PYGSF in terms of colony diameter of the different isolates evaluated. In these culture media, all L. chapmanii isolates had the highest growth (45 mm2) after 7 days. The nine isolates tested produced the greatest number of zoospores in FORT(agar) medium 3 (1 × 106 zoospores/ml). Medium YPSS exhibited significant differences (P <0.0001) compared to other culture media with respect to maintenance of virulence and infectivity of the isolates, producing 100% larval mortality after 48 h of inoculation. Thus, for the cultivation and maintenance of L. chapmanii isolates, the medium of choice among those tested is FORT(agar), as it is both inexpensive to prepare and ensures optimal colony development, extremely high zoospore production, and excellent maintenance of fungal infectivity.Centro de Estudios Parasitológicos y de Vectores2011-02-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf991-997http://sedici.unlp.edu.ar/handle/10915/131877enginfo:eu-repo/semantics/altIdentifier/issn/1590-4261info:eu-repo/semantics/altIdentifier/issn/1869-2044info:eu-repo/semantics/altIdentifier/doi/10.1007/s13213-011-0232-7info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-11-12T10:55:59Zoai:sedici.unlp.edu.ar:10915/131877Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-11-12 10:55:59.914SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Screening for a culture medium yielding optimal colony growth, zoospore yield and infectivity of different isolates of Leptolegnia chapmanii (Straminipila: Peronosporomycetes)
title Screening for a culture medium yielding optimal colony growth, zoospore yield and infectivity of different isolates of Leptolegnia chapmanii (Straminipila: Peronosporomycetes)
spellingShingle Screening for a culture medium yielding optimal colony growth, zoospore yield and infectivity of different isolates of Leptolegnia chapmanii (Straminipila: Peronosporomycetes)
Pelizza, Sebastián Alberto
Ciencias Naturales
Ciencias Agrarias
Leptolegnia chapmanii
Isolate
Culture medium
Aedes aegypti
title_short Screening for a culture medium yielding optimal colony growth, zoospore yield and infectivity of different isolates of Leptolegnia chapmanii (Straminipila: Peronosporomycetes)
title_full Screening for a culture medium yielding optimal colony growth, zoospore yield and infectivity of different isolates of Leptolegnia chapmanii (Straminipila: Peronosporomycetes)
title_fullStr Screening for a culture medium yielding optimal colony growth, zoospore yield and infectivity of different isolates of Leptolegnia chapmanii (Straminipila: Peronosporomycetes)
title_full_unstemmed Screening for a culture medium yielding optimal colony growth, zoospore yield and infectivity of different isolates of Leptolegnia chapmanii (Straminipila: Peronosporomycetes)
title_sort Screening for a culture medium yielding optimal colony growth, zoospore yield and infectivity of different isolates of Leptolegnia chapmanii (Straminipila: Peronosporomycetes)
dc.creator.none.fl_str_mv Pelizza, Sebastián Alberto
Cabello, Marta Noemí
Tranchida, María Cecilia
Scorsetti, Ana Clara
Bisaro, Vilma
author Pelizza, Sebastián Alberto
author_facet Pelizza, Sebastián Alberto
Cabello, Marta Noemí
Tranchida, María Cecilia
Scorsetti, Ana Clara
Bisaro, Vilma
author_role author
author2 Cabello, Marta Noemí
Tranchida, María Cecilia
Scorsetti, Ana Clara
Bisaro, Vilma
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Naturales
Ciencias Agrarias
Leptolegnia chapmanii
Isolate
Culture medium
Aedes aegypti
topic Ciencias Naturales
Ciencias Agrarias
Leptolegnia chapmanii
Isolate
Culture medium
Aedes aegypti
dc.description.none.fl_txt_mv Leptolegnia chapmanii is an aquatic fungus that has demonstrated marked pathogenicity towards the larvae of a number of mosquito species with little or no effect on non-target insects. The aim of this work was to determine the best culture medium for colony growth, and production and infectivity of zoospores among different isolates of L. chapmanii. No significant differences (P >0.01) were observed for the media FORT(agar), PYG, YPSS, and PYGSF in terms of colony diameter of the different isolates evaluated. In these culture media, all L. chapmanii isolates had the highest growth (45 mm2) after 7 days. The nine isolates tested produced the greatest number of zoospores in FORT(agar) medium 3 (1 × 106 zoospores/ml). Medium YPSS exhibited significant differences (P <0.0001) compared to other culture media with respect to maintenance of virulence and infectivity of the isolates, producing 100% larval mortality after 48 h of inoculation. Thus, for the cultivation and maintenance of L. chapmanii isolates, the medium of choice among those tested is FORT(agar), as it is both inexpensive to prepare and ensures optimal colony development, extremely high zoospore production, and excellent maintenance of fungal infectivity.
Centro de Estudios Parasitológicos y de Vectores
description Leptolegnia chapmanii is an aquatic fungus that has demonstrated marked pathogenicity towards the larvae of a number of mosquito species with little or no effect on non-target insects. The aim of this work was to determine the best culture medium for colony growth, and production and infectivity of zoospores among different isolates of L. chapmanii. No significant differences (P >0.01) were observed for the media FORT(agar), PYG, YPSS, and PYGSF in terms of colony diameter of the different isolates evaluated. In these culture media, all L. chapmanii isolates had the highest growth (45 mm2) after 7 days. The nine isolates tested produced the greatest number of zoospores in FORT(agar) medium 3 (1 × 106 zoospores/ml). Medium YPSS exhibited significant differences (P <0.0001) compared to other culture media with respect to maintenance of virulence and infectivity of the isolates, producing 100% larval mortality after 48 h of inoculation. Thus, for the cultivation and maintenance of L. chapmanii isolates, the medium of choice among those tested is FORT(agar), as it is both inexpensive to prepare and ensures optimal colony development, extremely high zoospore production, and excellent maintenance of fungal infectivity.
publishDate 2011
dc.date.none.fl_str_mv 2011-02-24
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/131877
url http://sedici.unlp.edu.ar/handle/10915/131877
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1590-4261
info:eu-repo/semantics/altIdentifier/issn/1869-2044
info:eu-repo/semantics/altIdentifier/doi/10.1007/s13213-011-0232-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
991-997
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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