Supplementary material for “Role of Tad Pili during the transition from Planktonic to Biofilm State in Bradyrhizobium diazoefficiens USDA 110”

Autores
Iglesias, Julián; Colla, Delfina; Serrangelli, Juan Simón; Lozano, Mauricio Javier; Falduti, Ornela; Brignoli, Damián; Medici, Ian; Althabeigoiti, María Julia; Lodeiro, Aníbal Roberto; Abdian, Lorena Patricia; Paczia, Nicole; Becker, Anke; Soler Bistue, Alfonso; Pérez Giménez, Julieta; Mongiardini, Elías Javier
Año de publicación
2025
Idioma
español castellano
Tipo de recurso
conjunto de datos
Estado
versión publicada
Descripción
Free-living soil bacteria can exist in two main states: planktonic, as motile single cells, or sessile, within biofilms. In biofilms, bacterial cells are embedded in an extracellular matrix that provides protection from environmental stresses and enhances long-term survival. The transition from planktonic to biofilm states sometimes involves surface sensing and attachment, processes com-monly mediated by flagella and pili. In this study, we investigated the role of Type IVc Tad pili in surface sensing, adhesion, and biofilm formation in Bradyrhizobium diazoefficiens, a nitrogen-fixing symbiont of soybean. Bioinformatic analyses revealed that Tad pili are widely distributed and high-ly conserved within the Bradyrhizobium genus. While pili deletion in other model organisms typi-cally reduces biofilm formation, we found that deletion of the most conserved genomic cluster encoding Tad pili in B. diazoefficiens led to increased adhesion to abiotic surfaces and impaired motility—indicative of a physiological shift toward a biofilm-associated state. These findings sug-gest that Tad pili may play a sensory or regulatory role, potentially influencing cell-cell or cell-matrix interactions. Furthermore, we identified a link between Tad pili and intracellular c-di-GMP levels. Together, these results highlight the critical role of Tad pili in the physiology of B. diazoeffi-ciens and offer new insights into bacterial surface adaptation, with potential applications in agri-culture and biotechnology. Understanding these mechanisms is essential for improving biofilm management strategies and developing new approaches to enhance bacterial survival in soil and inoculant formulations, ultimately optimizing legume symbiosis.
Fil: Iglesias, J. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Colla, D. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Serrangeli, J. S. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Lozano, M. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Falduti, O. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Brignoli, D. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Medici, J. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Argentina. Fil: Althabegoiti, M. J. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Lodeiro, A. R. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Abdian, P. L. Instituto de Microbiología y Zoología Agrícola. Argentina. Fil: Paczia, N. Max Planck Institute for Terrestrial Microbiology. Alemania. Fil: Becker, A. Center for Synthetic Microbiology. Alemania. Fil: Soler Bistué, A. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Argentina. Fil: Mongiardini, E. J. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina.
Instituto de Biotecnología y Biología Molecular
Materia
Ciencias Exactas
Bradyrhizobium
Biofilm
Pili
Symbiosis
Soybean
c-di-GMP
Surface adhesion
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/186424
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/186424
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Supplementary material for “Role of Tad Pili during the transition from Planktonic to Biofilm State in Bradyrhizobium diazoefficiens USDA 110”Iglesias, JuliánColla, DelfinaSerrangelli, Juan SimónLozano, Mauricio JavierFalduti, OrnelaBrignoli, DamiánMedici, IanAlthabeigoiti, María JuliaLodeiro, Aníbal RobertoAbdian, Lorena PatriciaPaczia, NicoleBecker, AnkeSoler Bistue, AlfonsoPérez Giménez, JulietaMongiardini, Elías JavierCiencias Exactashttps://purl.org/becyt/ford/1.6BradyrhizobiumBiofilmPiliSymbiosisSoybeanc-di-GMPSurface adhesionFree-living soil bacteria can exist in two main states: planktonic, as motile single cells, or sessile, within biofilms. In biofilms, bacterial cells are embedded in an extracellular matrix that provides protection from environmental stresses and enhances long-term survival. The transition from planktonic to biofilm states sometimes involves surface sensing and attachment, processes com-monly mediated by flagella and pili. In this study, we investigated the role of Type IVc Tad pili in surface sensing, adhesion, and biofilm formation in Bradyrhizobium diazoefficiens, a nitrogen-fixing symbiont of soybean. Bioinformatic analyses revealed that Tad pili are widely distributed and high-ly conserved within the Bradyrhizobium genus. While pili deletion in other model organisms typi-cally reduces biofilm formation, we found that deletion of the most conserved genomic cluster encoding Tad pili in B. diazoefficiens led to increased adhesion to abiotic surfaces and impaired motility—indicative of a physiological shift toward a biofilm-associated state. These findings sug-gest that Tad pili may play a sensory or regulatory role, potentially influencing cell-cell or cell-matrix interactions. Furthermore, we identified a link between Tad pili and intracellular c-di-GMP levels. Together, these results highlight the critical role of Tad pili in the physiology of B. diazoeffi-ciens and offer new insights into bacterial surface adaptation, with potential applications in agri-culture and biotechnology. Understanding these mechanisms is essential for improving biofilm management strategies and developing new approaches to enhance bacterial survival in soil and inoculant formulations, ultimately optimizing legume symbiosis.Fil: Iglesias, J. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Colla, D. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Serrangeli, J. S. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Lozano, M. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Falduti, O. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Brignoli, D. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Medici, J. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Argentina. Fil: Althabegoiti, M. J. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Lodeiro, A. R. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Abdian, P. L. Instituto de Microbiología y Zoología Agrícola. Argentina. Fil: Paczia, N. Max Planck Institute for Terrestrial Microbiology. Alemania. Fil: Becker, A. Center for Synthetic Microbiology. Alemania. Fil: Soler Bistué, A. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Argentina. Fil: Mongiardini, E. J. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina.Instituto de Biotecnología y Biología Molecular2025-10-24info:eu-repo/semantics/publishedVersionConjunto de datoshttp://purl.org/coar/resource_type/c_ddb1info:ar-repo/semantics/conjuntoDeDatosinfo:eu-repo/semantics/dataSetapplication/zipLos materiales y técnicas que se emplearon para la obtención y análisis de los datos que se adjuntan en este material suplementario se pueden encontrar en la sección materiales y métodos subida como preprint en el sitio bioRxiv (ver "Documentos relacionados").http://sedici.unlp.edu.ar/handle/10915/186424https://doi.org/10.35537/10915/186424spainfo:eu-repo/semantics/reference/doi/10.1101/2025.01.14.633045info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-11-05T13:30:21Zoai:sedici.unlp.edu.ar:10915/186424Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-11-05 13:30:21.845SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Supplementary material for “Role of Tad Pili during the transition from Planktonic to Biofilm State in Bradyrhizobium diazoefficiens USDA 110”
title Supplementary material for “Role of Tad Pili during the transition from Planktonic to Biofilm State in Bradyrhizobium diazoefficiens USDA 110”
spellingShingle Supplementary material for “Role of Tad Pili during the transition from Planktonic to Biofilm State in Bradyrhizobium diazoefficiens USDA 110”
Iglesias, Julián
Ciencias Exactas
Bradyrhizobium
Biofilm
Pili
Symbiosis
Soybean
c-di-GMP
Surface adhesion
title_short Supplementary material for “Role of Tad Pili during the transition from Planktonic to Biofilm State in Bradyrhizobium diazoefficiens USDA 110”
title_full Supplementary material for “Role of Tad Pili during the transition from Planktonic to Biofilm State in Bradyrhizobium diazoefficiens USDA 110”
title_fullStr Supplementary material for “Role of Tad Pili during the transition from Planktonic to Biofilm State in Bradyrhizobium diazoefficiens USDA 110”
title_full_unstemmed Supplementary material for “Role of Tad Pili during the transition from Planktonic to Biofilm State in Bradyrhizobium diazoefficiens USDA 110”
title_sort Supplementary material for “Role of Tad Pili during the transition from Planktonic to Biofilm State in Bradyrhizobium diazoefficiens USDA 110”
dc.creator.none.fl_str_mv Iglesias, Julián
Colla, Delfina
Serrangelli, Juan Simón
Lozano, Mauricio Javier
Falduti, Ornela
Brignoli, Damián
Medici, Ian
Althabeigoiti, María Julia
Lodeiro, Aníbal Roberto
Abdian, Lorena Patricia
Paczia, Nicole
Becker, Anke
Soler Bistue, Alfonso
Pérez Giménez, Julieta
Mongiardini, Elías Javier
author Iglesias, Julián
author_facet Iglesias, Julián
Colla, Delfina
Serrangelli, Juan Simón
Lozano, Mauricio Javier
Falduti, Ornela
Brignoli, Damián
Medici, Ian
Althabeigoiti, María Julia
Lodeiro, Aníbal Roberto
Abdian, Lorena Patricia
Paczia, Nicole
Becker, Anke
Soler Bistue, Alfonso
Pérez Giménez, Julieta
Mongiardini, Elías Javier
author_role author
author2 Colla, Delfina
Serrangelli, Juan Simón
Lozano, Mauricio Javier
Falduti, Ornela
Brignoli, Damián
Medici, Ian
Althabeigoiti, María Julia
Lodeiro, Aníbal Roberto
Abdian, Lorena Patricia
Paczia, Nicole
Becker, Anke
Soler Bistue, Alfonso
Pérez Giménez, Julieta
Mongiardini, Elías Javier
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Bradyrhizobium
Biofilm
Pili
Symbiosis
Soybean
c-di-GMP
Surface adhesion
topic Ciencias Exactas
Bradyrhizobium
Biofilm
Pili
Symbiosis
Soybean
c-di-GMP
Surface adhesion
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
dc.description.none.fl_txt_mv Free-living soil bacteria can exist in two main states: planktonic, as motile single cells, or sessile, within biofilms. In biofilms, bacterial cells are embedded in an extracellular matrix that provides protection from environmental stresses and enhances long-term survival. The transition from planktonic to biofilm states sometimes involves surface sensing and attachment, processes com-monly mediated by flagella and pili. In this study, we investigated the role of Type IVc Tad pili in surface sensing, adhesion, and biofilm formation in Bradyrhizobium diazoefficiens, a nitrogen-fixing symbiont of soybean. Bioinformatic analyses revealed that Tad pili are widely distributed and high-ly conserved within the Bradyrhizobium genus. While pili deletion in other model organisms typi-cally reduces biofilm formation, we found that deletion of the most conserved genomic cluster encoding Tad pili in B. diazoefficiens led to increased adhesion to abiotic surfaces and impaired motility—indicative of a physiological shift toward a biofilm-associated state. These findings sug-gest that Tad pili may play a sensory or regulatory role, potentially influencing cell-cell or cell-matrix interactions. Furthermore, we identified a link between Tad pili and intracellular c-di-GMP levels. Together, these results highlight the critical role of Tad pili in the physiology of B. diazoeffi-ciens and offer new insights into bacterial surface adaptation, with potential applications in agri-culture and biotechnology. Understanding these mechanisms is essential for improving biofilm management strategies and developing new approaches to enhance bacterial survival in soil and inoculant formulations, ultimately optimizing legume symbiosis.
Fil: Iglesias, J. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Colla, D. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Serrangeli, J. S. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Lozano, M. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Falduti, O. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Brignoli, D. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Medici, J. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Argentina. Fil: Althabegoiti, M. J. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Lodeiro, A. R. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina. Fil: Abdian, P. L. Instituto de Microbiología y Zoología Agrícola. Argentina. Fil: Paczia, N. Max Planck Institute for Terrestrial Microbiology. Alemania. Fil: Becker, A. Center for Synthetic Microbiology. Alemania. Fil: Soler Bistué, A. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Argentina. Fil: Mongiardini, E. J. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina.
Instituto de Biotecnología y Biología Molecular
description Free-living soil bacteria can exist in two main states: planktonic, as motile single cells, or sessile, within biofilms. In biofilms, bacterial cells are embedded in an extracellular matrix that provides protection from environmental stresses and enhances long-term survival. The transition from planktonic to biofilm states sometimes involves surface sensing and attachment, processes com-monly mediated by flagella and pili. In this study, we investigated the role of Type IVc Tad pili in surface sensing, adhesion, and biofilm formation in Bradyrhizobium diazoefficiens, a nitrogen-fixing symbiont of soybean. Bioinformatic analyses revealed that Tad pili are widely distributed and high-ly conserved within the Bradyrhizobium genus. While pili deletion in other model organisms typi-cally reduces biofilm formation, we found that deletion of the most conserved genomic cluster encoding Tad pili in B. diazoefficiens led to increased adhesion to abiotic surfaces and impaired motility—indicative of a physiological shift toward a biofilm-associated state. These findings sug-gest that Tad pili may play a sensory or regulatory role, potentially influencing cell-cell or cell-matrix interactions. Furthermore, we identified a link between Tad pili and intracellular c-di-GMP levels. Together, these results highlight the critical role of Tad pili in the physiology of B. diazoeffi-ciens and offer new insights into bacterial surface adaptation, with potential applications in agri-culture and biotechnology. Understanding these mechanisms is essential for improving biofilm management strategies and developing new approaches to enhance bacterial survival in soil and inoculant formulations, ultimately optimizing legume symbiosis.
publishDate 2025
dc.date.none.fl_str_mv 2025-10-24
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/186424
https://doi.org/10.35537/10915/186424
url http://sedici.unlp.edu.ar/handle/10915/186424
https://doi.org/10.35537/10915/186424
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/reference/doi/10.1101/2025.01.14.633045
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
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dc.format.none.fl_str_mv application/zip
Los materiales y técnicas que se emplearon para la obtención y análisis de los datos que se adjuntan en este material suplementario se pueden encontrar en la sección materiales y métodos subida como preprint en el sitio bioRxiv (ver "Documentos relacionados").
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