On the Thermal Stability of DNA in Solution of Mixed Solvents
- Autores
- Xammar Oro, Juan Roberto de; Grigera, José Raúl
- Año de publicación
- 1995
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The study of the changes in UV absorbance of DNA solutions in water/dioxane and water/ethylene glycol mixture at different concentrations shows that the thermal denaturation of DNA is sensitive to the electrical permittivity of the media and the water content. At relative low concentrations of co-solvent the dominant feature is the electrical permittivity. When water content is lower than a critical value, the electrical permittivity is no longer the determinant of the denaturation temperature but the partial volume fraction of water. The critical water content is about 0.69 partial volume fraction of water.
Instituto de Física de Líquidos y Sistemas Biológicos - Materia
-
Física
DNA stability
hydrophobic interactions
water stabilization effects
electrostatic interactions in DNA - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/139453
Ver los metadatos del registro completo
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On the Thermal Stability of DNA in Solution of Mixed SolventsXammar Oro, Juan Roberto deGrigera, José RaúlFísicaDNA stabilityhydrophobic interactionswater stabilization effectselectrostatic interactions in DNAThe study of the changes in UV absorbance of DNA solutions in water/dioxane and water/ethylene glycol mixture at different concentrations shows that the thermal denaturation of DNA is sensitive to the electrical permittivity of the media and the water content. At relative low concentrations of co-solvent the dominant feature is the electrical permittivity. When water content is lower than a critical value, the electrical permittivity is no longer the determinant of the denaturation temperature but the partial volume fraction of water. The critical water content is about 0.69 partial volume fraction of water.Instituto de Física de Líquidos y Sistemas Biológicos1995info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf151-154http://sedici.unlp.edu.ar/handle/10915/139453enginfo:eu-repo/semantics/altIdentifier/issn/0092-0606info:eu-repo/semantics/altIdentifier/issn/1573-0689info:eu-repo/semantics/altIdentifier/doi/10.1007/bf00712343info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:22Zoai:sedici.unlp.edu.ar:10915/139453Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:23.043SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
On the Thermal Stability of DNA in Solution of Mixed Solvents |
| title |
On the Thermal Stability of DNA in Solution of Mixed Solvents |
| spellingShingle |
On the Thermal Stability of DNA in Solution of Mixed Solvents Xammar Oro, Juan Roberto de Física DNA stability hydrophobic interactions water stabilization effects electrostatic interactions in DNA |
| title_short |
On the Thermal Stability of DNA in Solution of Mixed Solvents |
| title_full |
On the Thermal Stability of DNA in Solution of Mixed Solvents |
| title_fullStr |
On the Thermal Stability of DNA in Solution of Mixed Solvents |
| title_full_unstemmed |
On the Thermal Stability of DNA in Solution of Mixed Solvents |
| title_sort |
On the Thermal Stability of DNA in Solution of Mixed Solvents |
| dc.creator.none.fl_str_mv |
Xammar Oro, Juan Roberto de Grigera, José Raúl |
| author |
Xammar Oro, Juan Roberto de |
| author_facet |
Xammar Oro, Juan Roberto de Grigera, José Raúl |
| author_role |
author |
| author2 |
Grigera, José Raúl |
| author2_role |
author |
| dc.subject.none.fl_str_mv |
Física DNA stability hydrophobic interactions water stabilization effects electrostatic interactions in DNA |
| topic |
Física DNA stability hydrophobic interactions water stabilization effects electrostatic interactions in DNA |
| dc.description.none.fl_txt_mv |
The study of the changes in UV absorbance of DNA solutions in water/dioxane and water/ethylene glycol mixture at different concentrations shows that the thermal denaturation of DNA is sensitive to the electrical permittivity of the media and the water content. At relative low concentrations of co-solvent the dominant feature is the electrical permittivity. When water content is lower than a critical value, the electrical permittivity is no longer the determinant of the denaturation temperature but the partial volume fraction of water. The critical water content is about 0.69 partial volume fraction of water. Instituto de Física de Líquidos y Sistemas Biológicos |
| description |
The study of the changes in UV absorbance of DNA solutions in water/dioxane and water/ethylene glycol mixture at different concentrations shows that the thermal denaturation of DNA is sensitive to the electrical permittivity of the media and the water content. At relative low concentrations of co-solvent the dominant feature is the electrical permittivity. When water content is lower than a critical value, the electrical permittivity is no longer the determinant of the denaturation temperature but the partial volume fraction of water. The critical water content is about 0.69 partial volume fraction of water. |
| publishDate |
1995 |
| dc.date.none.fl_str_mv |
1995 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/139453 |
| url |
http://sedici.unlp.edu.ar/handle/10915/139453 |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/0092-0606 info:eu-repo/semantics/altIdentifier/issn/1573-0689 info:eu-repo/semantics/altIdentifier/doi/10.1007/bf00712343 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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openAccess |
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http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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application/pdf 151-154 |
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SEDICI (UNLP) - Universidad Nacional de La Plata |
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