Air drying technique for cytogenetic studies in freshwater planarians (Platyhelminthes)

Autores
Margaría, Cecilia Beatriz; Dulout, Fernando Noel
Año de publicación
1997
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cytogenetic studies in seawater or land planarians have been generally performed by means of the squash technique (Ball and De Vries 1983; Curini-Galletti et at., 1985, 1989a, b; Martens et at. 1989a, b; Oki et at. 1991; Puccinelli and Curum-Galletti 1987; Tamura et at. 1991). However, the quality of chromosome preparations obtained by squashing in terms of spreading and detritus background is not as good as that obtained by using the air drying technique in other taxa. In addition, few information is available about citogenetic techniques for freshwater invertebrates. The air drying technique (Rothfelds and Siminovitch 1958) was proposed for cytogenetic analysis of mammalian bone marrow cells and it included previous tissue disgregation. For nearly forty years the technique became widely used, with several modifications, for cytogenetic studies even in plant cells. This paper describes the modifications made in the technique for the analysis of planarians chromosomes.
Facultad de Ciencias Naturales y Museo
Facultad de Ciencias Veterinarias
Materia
Biología
Air drying technique
Planarians chromosomes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/122975
Acceder
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network_name_str SEDICI (UNLP)
spelling Air drying technique for cytogenetic studies in freshwater planarians (Platyhelminthes)Margaría, Cecilia BeatrizDulout, Fernando NoelBiologíaAir drying techniquePlanarians chromosomesCytogenetic studies in seawater or land planarians have been generally performed by means of the squash technique (Ball and De Vries 1983; Curini-Galletti et at., 1985, 1989a, b; Martens et at. 1989a, b; Oki et at. 1991; Puccinelli and Curum-Galletti 1987; Tamura et at. 1991). However, the quality of chromosome preparations obtained by squashing in terms of spreading and detritus background is not as good as that obtained by using the air drying technique in other taxa. In addition, few information is available about citogenetic techniques for freshwater invertebrates. The air drying technique (Rothfelds and Siminovitch 1958) was proposed for cytogenetic analysis of mammalian bone marrow cells and it included previous tissue disgregation. For nearly forty years the technique became widely used, with several modifications, for cytogenetic studies even in plant cells. This paper describes the modifications made in the technique for the analysis of planarians chromosomes.Facultad de Ciencias Naturales y MuseoFacultad de Ciencias Veterinarias1997info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf31-33http://sedici.unlp.edu.ar/handle/10915/122975enginfo:eu-repo/semantics/altIdentifier/issn/0008-7114info:eu-repo/semantics/altIdentifier/issn/2165-5391info:eu-repo/semantics/altIdentifier/doi/10.1080/00087114.1997.10797382info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:29:14Zoai:sedici.unlp.edu.ar:10915/122975Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:29:15.139SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Air drying technique for cytogenetic studies in freshwater planarians (Platyhelminthes)
title Air drying technique for cytogenetic studies in freshwater planarians (Platyhelminthes)
spellingShingle Air drying technique for cytogenetic studies in freshwater planarians (Platyhelminthes)
Margaría, Cecilia Beatriz
Biología
Air drying technique
Planarians chromosomes
title_short Air drying technique for cytogenetic studies in freshwater planarians (Platyhelminthes)
title_full Air drying technique for cytogenetic studies in freshwater planarians (Platyhelminthes)
title_fullStr Air drying technique for cytogenetic studies in freshwater planarians (Platyhelminthes)
title_full_unstemmed Air drying technique for cytogenetic studies in freshwater planarians (Platyhelminthes)
title_sort Air drying technique for cytogenetic studies in freshwater planarians (Platyhelminthes)
dc.creator.none.fl_str_mv Margaría, Cecilia Beatriz
Dulout, Fernando Noel
author Margaría, Cecilia Beatriz
author_facet Margaría, Cecilia Beatriz
Dulout, Fernando Noel
author_role author
author2 Dulout, Fernando Noel
author2_role author
dc.subject.none.fl_str_mv Biología
Air drying technique
Planarians chromosomes
topic Biología
Air drying technique
Planarians chromosomes
dc.description.none.fl_txt_mv Cytogenetic studies in seawater or land planarians have been generally performed by means of the squash technique (Ball and De Vries 1983; Curini-Galletti et at., 1985, 1989a, b; Martens et at. 1989a, b; Oki et at. 1991; Puccinelli and Curum-Galletti 1987; Tamura et at. 1991). However, the quality of chromosome preparations obtained by squashing in terms of spreading and detritus background is not as good as that obtained by using the air drying technique in other taxa. In addition, few information is available about citogenetic techniques for freshwater invertebrates. The air drying technique (Rothfelds and Siminovitch 1958) was proposed for cytogenetic analysis of mammalian bone marrow cells and it included previous tissue disgregation. For nearly forty years the technique became widely used, with several modifications, for cytogenetic studies even in plant cells. This paper describes the modifications made in the technique for the analysis of planarians chromosomes.
Facultad de Ciencias Naturales y Museo
Facultad de Ciencias Veterinarias
description Cytogenetic studies in seawater or land planarians have been generally performed by means of the squash technique (Ball and De Vries 1983; Curini-Galletti et at., 1985, 1989a, b; Martens et at. 1989a, b; Oki et at. 1991; Puccinelli and Curum-Galletti 1987; Tamura et at. 1991). However, the quality of chromosome preparations obtained by squashing in terms of spreading and detritus background is not as good as that obtained by using the air drying technique in other taxa. In addition, few information is available about citogenetic techniques for freshwater invertebrates. The air drying technique (Rothfelds and Siminovitch 1958) was proposed for cytogenetic analysis of mammalian bone marrow cells and it included previous tissue disgregation. For nearly forty years the technique became widely used, with several modifications, for cytogenetic studies even in plant cells. This paper describes the modifications made in the technique for the analysis of planarians chromosomes.
publishDate 1997
dc.date.none.fl_str_mv 1997
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/122975
url http://sedici.unlp.edu.ar/handle/10915/122975
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0008-7114
info:eu-repo/semantics/altIdentifier/issn/2165-5391
info:eu-repo/semantics/altIdentifier/doi/10.1080/00087114.1997.10797382
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
31-33
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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