Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients

Autores
Bernardo, David; Garrote, José Antonio; Allegretti, Yessica Lorena; Leon, Alberto J.; Gómez, E.; Bermejo-Martin, Jesus F.; Calvo, C.; Riestra, Sabino; Fernández-Salazar, Luis; Blanco-Quirós, Alfredo; Chirdo, Fernando Gabriel; Arranz, Eduardo
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The IL-15 triggering effect of gliadin is not exclusive to coeliac disease (CD) patients, whereas the secondary response is CD specific. We have studied the expression of the IL-15 receptor, and the IL-15 response upon stimulation, in non-CD and CD patients, and the possible existence of a lower immunological threshold in the latter. Forty-two CD patients (20 on a gluten-containing diet, GCD, and 22 on gluten-free diet, GFD) and 24 non-CD healthy individuals were studied. IL15Rα mRNA expression, and tissue characterization, were assayed in the duodenum. Biopsies from six CD patients on GFD and 10 non-CD individuals were studied in vitro using organ culture in basal conditions, as well as after IL-15 stimulation discarding basal IL-15 production. Secretion of immune mediators was measured in the culture supernatants. IL15Rα mRNA expression was increased in CD patients, as compared with non-CD controls (on GFD P = 0·0334, on GCD P = 0·0062, respectively), and confirmed also by immunofluorescence. No differences were found between CD patients on GFD and on GCD. After in vitro IL-15 stimulation, IL15Rα expression was only triggered in non-CD controls (P = 0·0313), though it remained increased in CD patients. Moreover, IL-15 induced a more intense immunological response in CD patients after triggering the production of both nitrites and IFNγ (P = 0·0313, P = 0·0313, respectively). Gliadin-induced IL15 has a lower response threshold in CD patients, leading to the production of other immune mediators and the development of the intestinal lesion, and thus magnifying its effects within the CD intestine.
Facultad de Ciencias Exactas
Laboratorio de Investigaciones del Sistema Inmune
Materia
Medicina
Ciencias Exactas
Biología
Coeliac disease
IL-15
IL15R
immune threshold
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/127321

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network_name_str SEDICI (UNLP)
spelling Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patientsBernardo, DavidGarrote, José AntonioAllegretti, Yessica LorenaLeon, Alberto J.Gómez, E.Bermejo-Martin, Jesus F.Calvo, C.Riestra, SabinoFernández-Salazar, LuisBlanco-Quirós, AlfredoChirdo, Fernando GabrielArranz, EduardoMedicinaCiencias ExactasBiologíaCoeliac diseaseIL-15IL15Rimmune thresholdThe IL-15 triggering effect of gliadin is not exclusive to coeliac disease (CD) patients, whereas the secondary response is CD specific. We have studied the expression of the IL-15 receptor, and the IL-15 response upon stimulation, in non-CD and CD patients, and the possible existence of a lower immunological threshold in the latter. Forty-two CD patients (20 on a gluten-containing diet, GCD, and 22 on gluten-free diet, GFD) and 24 non-CD healthy individuals were studied. IL15Rα mRNA expression, and tissue characterization, were assayed in the duodenum. Biopsies from six CD patients on GFD and 10 non-CD individuals were studied <i>in vitro</i> using organ culture in basal conditions, as well as after IL-15 stimulation discarding basal IL-15 production. Secretion of immune mediators was measured in the culture supernatants. IL15Rα mRNA expression was increased in CD patients, as compared with non-CD controls (on GFD P = 0·0334, on GCD P = 0·0062, respectively), and confirmed also by immunofluorescence. No differences were found between CD patients on GFD and on GCD. After <i>in vitro</i> IL-15 stimulation, IL15Rα expression was only triggered in non-CD controls (P = 0·0313), though it remained increased in CD patients. Moreover, IL-15 induced a more intense immunological response in CD patients after triggering the production of both nitrites and IFNγ (P = 0·0313, P = 0·0313, respectively). Gliadin-induced IL15 has a lower response threshold in CD patients, leading to the production of other immune mediators and the development of the intestinal lesion, and thus magnifying its effects within the CD intestine.Facultad de Ciencias ExactasLaboratorio de Investigaciones del Sistema Inmune2008-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf64-73http://sedici.unlp.edu.ar/handle/10915/127321enginfo:eu-repo/semantics/altIdentifier/issn/1365-2249info:eu-repo/semantics/altIdentifier/issn/0009-9104info:eu-repo/semantics/altIdentifier/pmid/18821940info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2249.2008.03743.xinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:30:42Zoai:sedici.unlp.edu.ar:10915/127321Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:30:43.257SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients
title Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients
spellingShingle Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients
Bernardo, David
Medicina
Ciencias Exactas
Biología
Coeliac disease
IL-15
IL15R
immune threshold
title_short Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients
title_full Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients
title_fullStr Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients
title_full_unstemmed Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients
title_sort Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients
dc.creator.none.fl_str_mv Bernardo, David
Garrote, José Antonio
Allegretti, Yessica Lorena
Leon, Alberto J.
Gómez, E.
Bermejo-Martin, Jesus F.
Calvo, C.
Riestra, Sabino
Fernández-Salazar, Luis
Blanco-Quirós, Alfredo
Chirdo, Fernando Gabriel
Arranz, Eduardo
author Bernardo, David
author_facet Bernardo, David
Garrote, José Antonio
Allegretti, Yessica Lorena
Leon, Alberto J.
Gómez, E.
Bermejo-Martin, Jesus F.
Calvo, C.
Riestra, Sabino
Fernández-Salazar, Luis
Blanco-Quirós, Alfredo
Chirdo, Fernando Gabriel
Arranz, Eduardo
author_role author
author2 Garrote, José Antonio
Allegretti, Yessica Lorena
Leon, Alberto J.
Gómez, E.
Bermejo-Martin, Jesus F.
Calvo, C.
Riestra, Sabino
Fernández-Salazar, Luis
Blanco-Quirós, Alfredo
Chirdo, Fernando Gabriel
Arranz, Eduardo
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Medicina
Ciencias Exactas
Biología
Coeliac disease
IL-15
IL15R
immune threshold
topic Medicina
Ciencias Exactas
Biología
Coeliac disease
IL-15
IL15R
immune threshold
dc.description.none.fl_txt_mv The IL-15 triggering effect of gliadin is not exclusive to coeliac disease (CD) patients, whereas the secondary response is CD specific. We have studied the expression of the IL-15 receptor, and the IL-15 response upon stimulation, in non-CD and CD patients, and the possible existence of a lower immunological threshold in the latter. Forty-two CD patients (20 on a gluten-containing diet, GCD, and 22 on gluten-free diet, GFD) and 24 non-CD healthy individuals were studied. IL15Rα mRNA expression, and tissue characterization, were assayed in the duodenum. Biopsies from six CD patients on GFD and 10 non-CD individuals were studied <i>in vitro</i> using organ culture in basal conditions, as well as after IL-15 stimulation discarding basal IL-15 production. Secretion of immune mediators was measured in the culture supernatants. IL15Rα mRNA expression was increased in CD patients, as compared with non-CD controls (on GFD P = 0·0334, on GCD P = 0·0062, respectively), and confirmed also by immunofluorescence. No differences were found between CD patients on GFD and on GCD. After <i>in vitro</i> IL-15 stimulation, IL15Rα expression was only triggered in non-CD controls (P = 0·0313), though it remained increased in CD patients. Moreover, IL-15 induced a more intense immunological response in CD patients after triggering the production of both nitrites and IFNγ (P = 0·0313, P = 0·0313, respectively). Gliadin-induced IL15 has a lower response threshold in CD patients, leading to the production of other immune mediators and the development of the intestinal lesion, and thus magnifying its effects within the CD intestine.
Facultad de Ciencias Exactas
Laboratorio de Investigaciones del Sistema Inmune
description The IL-15 triggering effect of gliadin is not exclusive to coeliac disease (CD) patients, whereas the secondary response is CD specific. We have studied the expression of the IL-15 receptor, and the IL-15 response upon stimulation, in non-CD and CD patients, and the possible existence of a lower immunological threshold in the latter. Forty-two CD patients (20 on a gluten-containing diet, GCD, and 22 on gluten-free diet, GFD) and 24 non-CD healthy individuals were studied. IL15Rα mRNA expression, and tissue characterization, were assayed in the duodenum. Biopsies from six CD patients on GFD and 10 non-CD individuals were studied <i>in vitro</i> using organ culture in basal conditions, as well as after IL-15 stimulation discarding basal IL-15 production. Secretion of immune mediators was measured in the culture supernatants. IL15Rα mRNA expression was increased in CD patients, as compared with non-CD controls (on GFD P = 0·0334, on GCD P = 0·0062, respectively), and confirmed also by immunofluorescence. No differences were found between CD patients on GFD and on GCD. After <i>in vitro</i> IL-15 stimulation, IL15Rα expression was only triggered in non-CD controls (P = 0·0313), though it remained increased in CD patients. Moreover, IL-15 induced a more intense immunological response in CD patients after triggering the production of both nitrites and IFNγ (P = 0·0313, P = 0·0313, respectively). Gliadin-induced IL15 has a lower response threshold in CD patients, leading to the production of other immune mediators and the development of the intestinal lesion, and thus magnifying its effects within the CD intestine.
publishDate 2008
dc.date.none.fl_str_mv 2008-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/127321
url http://sedici.unlp.edu.ar/handle/10915/127321
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/issn/0009-9104
info:eu-repo/semantics/altIdentifier/pmid/18821940
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2249.2008.03743.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
64-73
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