Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients
- Autores
- Bernardo, David; Garrote, José Antonio; Allegretti, Yessica Lorena; Leon, Alberto J.; Gómez, E.; Bermejo-Martin, Jesus F.; Calvo, C.; Riestra, Sabino; Fernández-Salazar, Luis; Blanco-Quirós, Alfredo; Chirdo, Fernando Gabriel; Arranz, Eduardo
- Año de publicación
- 2008
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The IL-15 triggering effect of gliadin is not exclusive to coeliac disease (CD) patients, whereas the secondary response is CD specific. We have studied the expression of the IL-15 receptor, and the IL-15 response upon stimulation, in non-CD and CD patients, and the possible existence of a lower immunological threshold in the latter. Forty-two CD patients (20 on a gluten-containing diet, GCD, and 22 on gluten-free diet, GFD) and 24 non-CD healthy individuals were studied. IL15Rα mRNA expression, and tissue characterization, were assayed in the duodenum. Biopsies from six CD patients on GFD and 10 non-CD individuals were studied in vitro using organ culture in basal conditions, as well as after IL-15 stimulation discarding basal IL-15 production. Secretion of immune mediators was measured in the culture supernatants. IL15Rα mRNA expression was increased in CD patients, as compared with non-CD controls (on GFD P = 0·0334, on GCD P = 0·0062, respectively), and confirmed also by immunofluorescence. No differences were found between CD patients on GFD and on GCD. After in vitro IL-15 stimulation, IL15Rα expression was only triggered in non-CD controls (P = 0·0313), though it remained increased in CD patients. Moreover, IL-15 induced a more intense immunological response in CD patients after triggering the production of both nitrites and IFNγ (P = 0·0313, P = 0·0313, respectively). Gliadin-induced IL15 has a lower response threshold in CD patients, leading to the production of other immune mediators and the development of the intestinal lesion, and thus magnifying its effects within the CD intestine.
Facultad de Ciencias Exactas
Laboratorio de Investigaciones del Sistema Inmune - Materia
-
Medicina
Ciencias Exactas
Biología
Coeliac disease
IL-15
IL15R
immune threshold - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/127321
Ver los metadatos del registro completo
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Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patientsBernardo, DavidGarrote, José AntonioAllegretti, Yessica LorenaLeon, Alberto J.Gómez, E.Bermejo-Martin, Jesus F.Calvo, C.Riestra, SabinoFernández-Salazar, LuisBlanco-Quirós, AlfredoChirdo, Fernando GabrielArranz, EduardoMedicinaCiencias ExactasBiologíaCoeliac diseaseIL-15IL15Rimmune thresholdThe IL-15 triggering effect of gliadin is not exclusive to coeliac disease (CD) patients, whereas the secondary response is CD specific. We have studied the expression of the IL-15 receptor, and the IL-15 response upon stimulation, in non-CD and CD patients, and the possible existence of a lower immunological threshold in the latter. Forty-two CD patients (20 on a gluten-containing diet, GCD, and 22 on gluten-free diet, GFD) and 24 non-CD healthy individuals were studied. IL15Rα mRNA expression, and tissue characterization, were assayed in the duodenum. Biopsies from six CD patients on GFD and 10 non-CD individuals were studied <i>in vitro</i> using organ culture in basal conditions, as well as after IL-15 stimulation discarding basal IL-15 production. Secretion of immune mediators was measured in the culture supernatants. IL15Rα mRNA expression was increased in CD patients, as compared with non-CD controls (on GFD P = 0·0334, on GCD P = 0·0062, respectively), and confirmed also by immunofluorescence. No differences were found between CD patients on GFD and on GCD. After <i>in vitro</i> IL-15 stimulation, IL15Rα expression was only triggered in non-CD controls (P = 0·0313), though it remained increased in CD patients. Moreover, IL-15 induced a more intense immunological response in CD patients after triggering the production of both nitrites and IFNγ (P = 0·0313, P = 0·0313, respectively). Gliadin-induced IL15 has a lower response threshold in CD patients, leading to the production of other immune mediators and the development of the intestinal lesion, and thus magnifying its effects within the CD intestine.Facultad de Ciencias ExactasLaboratorio de Investigaciones del Sistema Inmune2008-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf64-73http://sedici.unlp.edu.ar/handle/10915/127321enginfo:eu-repo/semantics/altIdentifier/issn/1365-2249info:eu-repo/semantics/altIdentifier/issn/0009-9104info:eu-repo/semantics/altIdentifier/pmid/18821940info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2249.2008.03743.xinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:11:37Zoai:sedici.unlp.edu.ar:10915/127321Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:11:37.45SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients |
| title |
Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients |
| spellingShingle |
Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients Bernardo, David Medicina Ciencias Exactas Biología Coeliac disease IL-15 IL15R immune threshold |
| title_short |
Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients |
| title_full |
Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients |
| title_fullStr |
Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients |
| title_full_unstemmed |
Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients |
| title_sort |
Higher constitutive IL15Rα expression and lower IL‐15 response threshold in coeliac disease patients |
| dc.creator.none.fl_str_mv |
Bernardo, David Garrote, José Antonio Allegretti, Yessica Lorena Leon, Alberto J. Gómez, E. Bermejo-Martin, Jesus F. Calvo, C. Riestra, Sabino Fernández-Salazar, Luis Blanco-Quirós, Alfredo Chirdo, Fernando Gabriel Arranz, Eduardo |
| author |
Bernardo, David |
| author_facet |
Bernardo, David Garrote, José Antonio Allegretti, Yessica Lorena Leon, Alberto J. Gómez, E. Bermejo-Martin, Jesus F. Calvo, C. Riestra, Sabino Fernández-Salazar, Luis Blanco-Quirós, Alfredo Chirdo, Fernando Gabriel Arranz, Eduardo |
| author_role |
author |
| author2 |
Garrote, José Antonio Allegretti, Yessica Lorena Leon, Alberto J. Gómez, E. Bermejo-Martin, Jesus F. Calvo, C. Riestra, Sabino Fernández-Salazar, Luis Blanco-Quirós, Alfredo Chirdo, Fernando Gabriel Arranz, Eduardo |
| author2_role |
author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Medicina Ciencias Exactas Biología Coeliac disease IL-15 IL15R immune threshold |
| topic |
Medicina Ciencias Exactas Biología Coeliac disease IL-15 IL15R immune threshold |
| dc.description.none.fl_txt_mv |
The IL-15 triggering effect of gliadin is not exclusive to coeliac disease (CD) patients, whereas the secondary response is CD specific. We have studied the expression of the IL-15 receptor, and the IL-15 response upon stimulation, in non-CD and CD patients, and the possible existence of a lower immunological threshold in the latter. Forty-two CD patients (20 on a gluten-containing diet, GCD, and 22 on gluten-free diet, GFD) and 24 non-CD healthy individuals were studied. IL15Rα mRNA expression, and tissue characterization, were assayed in the duodenum. Biopsies from six CD patients on GFD and 10 non-CD individuals were studied <i>in vitro</i> using organ culture in basal conditions, as well as after IL-15 stimulation discarding basal IL-15 production. Secretion of immune mediators was measured in the culture supernatants. IL15Rα mRNA expression was increased in CD patients, as compared with non-CD controls (on GFD P = 0·0334, on GCD P = 0·0062, respectively), and confirmed also by immunofluorescence. No differences were found between CD patients on GFD and on GCD. After <i>in vitro</i> IL-15 stimulation, IL15Rα expression was only triggered in non-CD controls (P = 0·0313), though it remained increased in CD patients. Moreover, IL-15 induced a more intense immunological response in CD patients after triggering the production of both nitrites and IFNγ (P = 0·0313, P = 0·0313, respectively). Gliadin-induced IL15 has a lower response threshold in CD patients, leading to the production of other immune mediators and the development of the intestinal lesion, and thus magnifying its effects within the CD intestine. Facultad de Ciencias Exactas Laboratorio de Investigaciones del Sistema Inmune |
| description |
The IL-15 triggering effect of gliadin is not exclusive to coeliac disease (CD) patients, whereas the secondary response is CD specific. We have studied the expression of the IL-15 receptor, and the IL-15 response upon stimulation, in non-CD and CD patients, and the possible existence of a lower immunological threshold in the latter. Forty-two CD patients (20 on a gluten-containing diet, GCD, and 22 on gluten-free diet, GFD) and 24 non-CD healthy individuals were studied. IL15Rα mRNA expression, and tissue characterization, were assayed in the duodenum. Biopsies from six CD patients on GFD and 10 non-CD individuals were studied <i>in vitro</i> using organ culture in basal conditions, as well as after IL-15 stimulation discarding basal IL-15 production. Secretion of immune mediators was measured in the culture supernatants. IL15Rα mRNA expression was increased in CD patients, as compared with non-CD controls (on GFD P = 0·0334, on GCD P = 0·0062, respectively), and confirmed also by immunofluorescence. No differences were found between CD patients on GFD and on GCD. After <i>in vitro</i> IL-15 stimulation, IL15Rα expression was only triggered in non-CD controls (P = 0·0313), though it remained increased in CD patients. Moreover, IL-15 induced a more intense immunological response in CD patients after triggering the production of both nitrites and IFNγ (P = 0·0313, P = 0·0313, respectively). Gliadin-induced IL15 has a lower response threshold in CD patients, leading to the production of other immune mediators and the development of the intestinal lesion, and thus magnifying its effects within the CD intestine. |
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2008 |
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2008-10 |
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eng |
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eng |
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