A Vaccine Based on Kunitz-Type Molecule Confers Protection Against <i>Fasciola hepatica</i> Challenge by Inducing IFN-γ and Antibody Immune Responses Through IL-17A Production
- Autores
- Silvane, Leonardo Micael; Celias, Daiana Pamela; Romagnoli, Pablo Alberto; Maletto, Belkys Angélica; Sánchez Vallecillo, María Fernanda; Chiapello, Laura Silvina; Palma, Santiago Daniel; Allemandi, Daniel Alberto; Sanabria, Rodrigo Eduardo Fabrizio; Pruzzo, César Iván; Motrán, Claudia Cristina; Cervi, Laura
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fasciola hepatica is helminth parasite found around the world that causes fasciolosis, a chronic disease affecting mainly cattle, sheep, and occasionally humans. Triclabendazole is the drug of choice to treat this parasite. However, the continuous use of this drug has led to the development of parasite resistance and, consequently, the limitation of its effectiveness. Hence, vaccination appears as an attractive option to develop. In this work, we evaluated the potential of F. hepatica Kunitz-type molecule (FhKTM) as an antigen formulated with a liquid crystal nanostructure formed by self-assembly of 6-O-ascorbyl palmitate ester (Coa-ASC16) and the synthetic oligodeoxynucleotide containing unmethylated cytosine-guanine motifs (CpG-ODN) during an experimental model of fasciolosis in mice, and we further dissected the immune response associated with host protection. Our results showed that immunization of mice with FhKTM/CpG-ODN/Coa-ASC16 induces protection against F. hepatica challenge by preventing liver damage and improving survival after F. hepatica infection. FhKTM/CpG-ODN/Coa-ASC16-immunized mice elicited potent IFN-γ and IL-17A with high levels of antigen-specific IgG1, IgG2a, and IgA serum antibodies. Strikingly, IL-17A blockade during infection decreased IgG2a and IgA antibody levels as well as IFN-γ production, leading to an increase in mortality of vaccinated mice. The present study highlights the potential of a new vaccine formulation to improve control and help the eradication of F. hepatica infection, with potential applications for natural hosts such as cattle and sheep.
Facultad de Ciencias Veterinarias - Materia
-
Ciencias Médicas
Ciencias Exactas
Veterinaria
Th17-dependent protection
nanostructure
ascorbyl palmitate
kunitz type molecule
vaccine
Fasciola hepatica - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/124518
Ver los metadatos del registro completo
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A Vaccine Based on Kunitz-Type Molecule Confers Protection Against <i>Fasciola hepatica</i> Challenge by Inducing IFN-γ and Antibody Immune Responses Through IL-17A ProductionSilvane, Leonardo MicaelCelias, Daiana PamelaRomagnoli, Pablo AlbertoMaletto, Belkys AngélicaSánchez Vallecillo, María FernandaChiapello, Laura SilvinaPalma, Santiago DanielAllemandi, Daniel AlbertoSanabria, Rodrigo Eduardo FabrizioPruzzo, César IvánMotrán, Claudia CristinaCervi, LauraCiencias MédicasCiencias ExactasVeterinariaTh17-dependent protectionnanostructureascorbyl palmitatekunitz type moleculevaccineFasciola hepatica<i>Fasciola hepatica</i> is helminth parasite found around the world that causes fasciolosis, a chronic disease affecting mainly cattle, sheep, and occasionally humans. Triclabendazole is the drug of choice to treat this parasite. However, the continuous use of this drug has led to the development of parasite resistance and, consequently, the limitation of its effectiveness. Hence, vaccination appears as an attractive option to develop. In this work, we evaluated the potential of <i>F. hepatica</i> Kunitz-type molecule (FhKTM) as an antigen formulated with a liquid crystal nanostructure formed by self-assembly of 6-O-ascorbyl palmitate ester (Coa-ASC16) and the synthetic oligodeoxynucleotide containing unmethylated cytosine-guanine motifs (CpG-ODN) during an experimental model of fasciolosis in mice, and we further dissected the immune response associated with host protection. Our results showed that immunization of mice with FhKTM/CpG-ODN/Coa-ASC16 induces protection against <i>F. hepatica</i> challenge by preventing liver damage and improving survival after <i>F. hepatica</i> infection. FhKTM/CpG-ODN/Coa-ASC16-immunized mice elicited potent IFN-γ and IL-17A with high levels of antigen-specific IgG1, IgG2a, and IgA serum antibodies. Strikingly, IL-17A blockade during infection decreased IgG2a and IgA antibody levels as well as IFN-γ production, leading to an increase in mortality of vaccinated mice. The present study highlights the potential of a new vaccine formulation to improve control and help the eradication of <i>F. hepatica</i> infection, with potential applications for natural hosts such as cattle and sheep.Facultad de Ciencias Veterinarias2020-10-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/124518enginfo:eu-repo/semantics/altIdentifier/issn/1664-3224info:eu-repo/semantics/altIdentifier/pmid/33193292info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2020.02087info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:01:54Zoai:sedici.unlp.edu.ar:10915/124518Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:01:54.889SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
A Vaccine Based on Kunitz-Type Molecule Confers Protection Against <i>Fasciola hepatica</i> Challenge by Inducing IFN-γ and Antibody Immune Responses Through IL-17A Production |
| title |
A Vaccine Based on Kunitz-Type Molecule Confers Protection Against <i>Fasciola hepatica</i> Challenge by Inducing IFN-γ and Antibody Immune Responses Through IL-17A Production |
| spellingShingle |
A Vaccine Based on Kunitz-Type Molecule Confers Protection Against <i>Fasciola hepatica</i> Challenge by Inducing IFN-γ and Antibody Immune Responses Through IL-17A Production Silvane, Leonardo Micael Ciencias Médicas Ciencias Exactas Veterinaria Th17-dependent protection nanostructure ascorbyl palmitate kunitz type molecule vaccine Fasciola hepatica |
| title_short |
A Vaccine Based on Kunitz-Type Molecule Confers Protection Against <i>Fasciola hepatica</i> Challenge by Inducing IFN-γ and Antibody Immune Responses Through IL-17A Production |
| title_full |
A Vaccine Based on Kunitz-Type Molecule Confers Protection Against <i>Fasciola hepatica</i> Challenge by Inducing IFN-γ and Antibody Immune Responses Through IL-17A Production |
| title_fullStr |
A Vaccine Based on Kunitz-Type Molecule Confers Protection Against <i>Fasciola hepatica</i> Challenge by Inducing IFN-γ and Antibody Immune Responses Through IL-17A Production |
| title_full_unstemmed |
A Vaccine Based on Kunitz-Type Molecule Confers Protection Against <i>Fasciola hepatica</i> Challenge by Inducing IFN-γ and Antibody Immune Responses Through IL-17A Production |
| title_sort |
A Vaccine Based on Kunitz-Type Molecule Confers Protection Against <i>Fasciola hepatica</i> Challenge by Inducing IFN-γ and Antibody Immune Responses Through IL-17A Production |
| dc.creator.none.fl_str_mv |
Silvane, Leonardo Micael Celias, Daiana Pamela Romagnoli, Pablo Alberto Maletto, Belkys Angélica Sánchez Vallecillo, María Fernanda Chiapello, Laura Silvina Palma, Santiago Daniel Allemandi, Daniel Alberto Sanabria, Rodrigo Eduardo Fabrizio Pruzzo, César Iván Motrán, Claudia Cristina Cervi, Laura |
| author |
Silvane, Leonardo Micael |
| author_facet |
Silvane, Leonardo Micael Celias, Daiana Pamela Romagnoli, Pablo Alberto Maletto, Belkys Angélica Sánchez Vallecillo, María Fernanda Chiapello, Laura Silvina Palma, Santiago Daniel Allemandi, Daniel Alberto Sanabria, Rodrigo Eduardo Fabrizio Pruzzo, César Iván Motrán, Claudia Cristina Cervi, Laura |
| author_role |
author |
| author2 |
Celias, Daiana Pamela Romagnoli, Pablo Alberto Maletto, Belkys Angélica Sánchez Vallecillo, María Fernanda Chiapello, Laura Silvina Palma, Santiago Daniel Allemandi, Daniel Alberto Sanabria, Rodrigo Eduardo Fabrizio Pruzzo, César Iván Motrán, Claudia Cristina Cervi, Laura |
| author2_role |
author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Ciencias Médicas Ciencias Exactas Veterinaria Th17-dependent protection nanostructure ascorbyl palmitate kunitz type molecule vaccine Fasciola hepatica |
| topic |
Ciencias Médicas Ciencias Exactas Veterinaria Th17-dependent protection nanostructure ascorbyl palmitate kunitz type molecule vaccine Fasciola hepatica |
| dc.description.none.fl_txt_mv |
<i>Fasciola hepatica</i> is helminth parasite found around the world that causes fasciolosis, a chronic disease affecting mainly cattle, sheep, and occasionally humans. Triclabendazole is the drug of choice to treat this parasite. However, the continuous use of this drug has led to the development of parasite resistance and, consequently, the limitation of its effectiveness. Hence, vaccination appears as an attractive option to develop. In this work, we evaluated the potential of <i>F. hepatica</i> Kunitz-type molecule (FhKTM) as an antigen formulated with a liquid crystal nanostructure formed by self-assembly of 6-O-ascorbyl palmitate ester (Coa-ASC16) and the synthetic oligodeoxynucleotide containing unmethylated cytosine-guanine motifs (CpG-ODN) during an experimental model of fasciolosis in mice, and we further dissected the immune response associated with host protection. Our results showed that immunization of mice with FhKTM/CpG-ODN/Coa-ASC16 induces protection against <i>F. hepatica</i> challenge by preventing liver damage and improving survival after <i>F. hepatica</i> infection. FhKTM/CpG-ODN/Coa-ASC16-immunized mice elicited potent IFN-γ and IL-17A with high levels of antigen-specific IgG1, IgG2a, and IgA serum antibodies. Strikingly, IL-17A blockade during infection decreased IgG2a and IgA antibody levels as well as IFN-γ production, leading to an increase in mortality of vaccinated mice. The present study highlights the potential of a new vaccine formulation to improve control and help the eradication of <i>F. hepatica</i> infection, with potential applications for natural hosts such as cattle and sheep. Facultad de Ciencias Veterinarias |
| description |
<i>Fasciola hepatica</i> is helminth parasite found around the world that causes fasciolosis, a chronic disease affecting mainly cattle, sheep, and occasionally humans. Triclabendazole is the drug of choice to treat this parasite. However, the continuous use of this drug has led to the development of parasite resistance and, consequently, the limitation of its effectiveness. Hence, vaccination appears as an attractive option to develop. In this work, we evaluated the potential of <i>F. hepatica</i> Kunitz-type molecule (FhKTM) as an antigen formulated with a liquid crystal nanostructure formed by self-assembly of 6-O-ascorbyl palmitate ester (Coa-ASC16) and the synthetic oligodeoxynucleotide containing unmethylated cytosine-guanine motifs (CpG-ODN) during an experimental model of fasciolosis in mice, and we further dissected the immune response associated with host protection. Our results showed that immunization of mice with FhKTM/CpG-ODN/Coa-ASC16 induces protection against <i>F. hepatica</i> challenge by preventing liver damage and improving survival after <i>F. hepatica</i> infection. FhKTM/CpG-ODN/Coa-ASC16-immunized mice elicited potent IFN-γ and IL-17A with high levels of antigen-specific IgG1, IgG2a, and IgA serum antibodies. Strikingly, IL-17A blockade during infection decreased IgG2a and IgA antibody levels as well as IFN-γ production, leading to an increase in mortality of vaccinated mice. The present study highlights the potential of a new vaccine formulation to improve control and help the eradication of <i>F. hepatica</i> infection, with potential applications for natural hosts such as cattle and sheep. |
| publishDate |
2020 |
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2020-10-20 |
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article |
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publishedVersion |
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| dc.language.none.fl_str_mv |
eng |
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eng |
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