Genomic analysis of an Argentinean isolate of <i>Spodoptera frugiperda granulovirus</i> reveals that various baculoviruses code for Lef-7 proteins with three F-box domains

Autores
Ferrelli, María Leticia; Pidre, Matías Luis; Ghiringhelli, Pablo Daniel; Torres, Sofía; Fabre, María Laura; Masson, Tomás; Cedola, Maia Tatiana; Sciocco, Alicia Inés; Romanowski, Víctor
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A new isolate of the Spodoptera frugiperda granulovirus, SfGV ARG, was completely sequenced and analyzed. The SfGV ARG genome is 139,812 bp long and encodes 151 putative open reading frames. Of these ORFs, 56 were found in betabaculoviruses, 19 of which are present only in GVs closely related to SfGV. Seven ORFs found homologs in this small GV group and also in noctuid NPVs. ORF066 codes a 74 amino acid protein, overlapped with nudix gene, with several homologs in baculovirus, found by tblastn search. Comparison with the genome of the Colombian isolate SfGV VG008 resulted in SfGV being 1101 bp smaller and lacking a homologue of VG008 ORF084, which codes for Lef-7. However, we found that ORF051 shows remote homology to Lef-7 proteins. Moreover, analysis of ORF051 along with Lef-7 proteins coded by a group of noctuid specific GVs and NPVs indicated that Lef-7 proteins coded by these viruses include three F-box domains in contrast to the single one reported for AcMNPV Lef-7. SfGV ARG genome also contains a split photolyase as a distinct feature not found in VG008. BlastX analysis revealed that a complete photolyase is coded considering a putative frameshift in a poly-A tract, which resembles known slippery sequences involved in programmed ribosome frameshifting.
Facultad de Ciencias Exactas
Instituto de Biotecnologia y Biologia Molecular
Materia
Ciencias Exactas
Biología
Baculovirus
Granulovirus
Spodoptera frugiperda
genome
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/104532
Acceder
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spelling Genomic analysis of an Argentinean isolate of <i>Spodoptera frugiperda granulovirus</i> reveals that various baculoviruses code for Lef-7 proteins with three F-box domainsFerrelli, María LeticiaPidre, Matías LuisGhiringhelli, Pablo DanielTorres, SofíaFabre, María LauraMasson, TomásCedola, Maia TatianaSciocco, Alicia InésRomanowski, VíctorCiencias ExactasBiologíaBaculovirusGranulovirusSpodoptera frugiperdagenomeA new isolate of the <i>Spodoptera frugiperda granulovirus</i>, SfGV ARG, was completely sequenced and analyzed. The SfGV ARG genome is 139,812 bp long and encodes 151 putative open reading frames. Of these ORFs, 56 were found in betabaculoviruses, 19 of which are present only in GVs closely related to SfGV. Seven ORFs found homologs in this small GV group and also in noctuid NPVs. ORF066 codes a 74 amino acid protein, overlapped with <i>nudix</i> gene, with several homologs in baculovirus, found by tblastn search. Comparison with the genome of the Colombian isolate SfGV VG008 resulted in SfGV being 1101 bp smaller and lacking a homologue of VG008 ORF084, which codes for Lef-7. However, we found that ORF051 shows remote homology to Lef-7 proteins. Moreover, analysis of ORF051 along with Lef-7 proteins coded by a group of noctuid specific GVs and NPVs indicated that Lef-7 proteins coded by these viruses include three F-box domains in contrast to the single one reported for AcMNPV Lef-7. SfGV ARG genome also contains a split photolyase as a distinct feature not found in VG008. BlastX analysis revealed that a complete photolyase is coded considering a putative frameshift in a poly-A tract, which resembles known slippery sequences involved in programmed ribosome frameshifting.Facultad de Ciencias ExactasInstituto de Biotecnologia y Biologia Molecular2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/104532enginfo:eu-repo/semantics/altIdentifier/url/http://hdl.handle.net/11336/96973info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0202598info:eu-repo/semantics/altIdentifier/issn/1932-6203info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0202598info:eu-repo/semantics/altIdentifier/hdl/11336/96973info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:22:47Zoai:sedici.unlp.edu.ar:10915/104532Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:22:48.227SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Genomic analysis of an Argentinean isolate of <i>Spodoptera frugiperda granulovirus</i> reveals that various baculoviruses code for Lef-7 proteins with three F-box domains
title Genomic analysis of an Argentinean isolate of <i>Spodoptera frugiperda granulovirus</i> reveals that various baculoviruses code for Lef-7 proteins with three F-box domains
spellingShingle Genomic analysis of an Argentinean isolate of <i>Spodoptera frugiperda granulovirus</i> reveals that various baculoviruses code for Lef-7 proteins with three F-box domains
Ferrelli, María Leticia
Ciencias Exactas
Biología
Baculovirus
Granulovirus
Spodoptera frugiperda
genome
title_short Genomic analysis of an Argentinean isolate of <i>Spodoptera frugiperda granulovirus</i> reveals that various baculoviruses code for Lef-7 proteins with three F-box domains
title_full Genomic analysis of an Argentinean isolate of <i>Spodoptera frugiperda granulovirus</i> reveals that various baculoviruses code for Lef-7 proteins with three F-box domains
title_fullStr Genomic analysis of an Argentinean isolate of <i>Spodoptera frugiperda granulovirus</i> reveals that various baculoviruses code for Lef-7 proteins with three F-box domains
title_full_unstemmed Genomic analysis of an Argentinean isolate of <i>Spodoptera frugiperda granulovirus</i> reveals that various baculoviruses code for Lef-7 proteins with three F-box domains
title_sort Genomic analysis of an Argentinean isolate of <i>Spodoptera frugiperda granulovirus</i> reveals that various baculoviruses code for Lef-7 proteins with three F-box domains
dc.creator.none.fl_str_mv Ferrelli, María Leticia
Pidre, Matías Luis
Ghiringhelli, Pablo Daniel
Torres, Sofía
Fabre, María Laura
Masson, Tomás
Cedola, Maia Tatiana
Sciocco, Alicia Inés
Romanowski, Víctor
author Ferrelli, María Leticia
author_facet Ferrelli, María Leticia
Pidre, Matías Luis
Ghiringhelli, Pablo Daniel
Torres, Sofía
Fabre, María Laura
Masson, Tomás
Cedola, Maia Tatiana
Sciocco, Alicia Inés
Romanowski, Víctor
author_role author
author2 Pidre, Matías Luis
Ghiringhelli, Pablo Daniel
Torres, Sofía
Fabre, María Laura
Masson, Tomás
Cedola, Maia Tatiana
Sciocco, Alicia Inés
Romanowski, Víctor
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Biología
Baculovirus
Granulovirus
Spodoptera frugiperda
genome
topic Ciencias Exactas
Biología
Baculovirus
Granulovirus
Spodoptera frugiperda
genome
dc.description.none.fl_txt_mv A new isolate of the <i>Spodoptera frugiperda granulovirus</i>, SfGV ARG, was completely sequenced and analyzed. The SfGV ARG genome is 139,812 bp long and encodes 151 putative open reading frames. Of these ORFs, 56 were found in betabaculoviruses, 19 of which are present only in GVs closely related to SfGV. Seven ORFs found homologs in this small GV group and also in noctuid NPVs. ORF066 codes a 74 amino acid protein, overlapped with <i>nudix</i> gene, with several homologs in baculovirus, found by tblastn search. Comparison with the genome of the Colombian isolate SfGV VG008 resulted in SfGV being 1101 bp smaller and lacking a homologue of VG008 ORF084, which codes for Lef-7. However, we found that ORF051 shows remote homology to Lef-7 proteins. Moreover, analysis of ORF051 along with Lef-7 proteins coded by a group of noctuid specific GVs and NPVs indicated that Lef-7 proteins coded by these viruses include three F-box domains in contrast to the single one reported for AcMNPV Lef-7. SfGV ARG genome also contains a split photolyase as a distinct feature not found in VG008. BlastX analysis revealed that a complete photolyase is coded considering a putative frameshift in a poly-A tract, which resembles known slippery sequences involved in programmed ribosome frameshifting.
Facultad de Ciencias Exactas
Instituto de Biotecnologia y Biologia Molecular
description A new isolate of the <i>Spodoptera frugiperda granulovirus</i>, SfGV ARG, was completely sequenced and analyzed. The SfGV ARG genome is 139,812 bp long and encodes 151 putative open reading frames. Of these ORFs, 56 were found in betabaculoviruses, 19 of which are present only in GVs closely related to SfGV. Seven ORFs found homologs in this small GV group and also in noctuid NPVs. ORF066 codes a 74 amino acid protein, overlapped with <i>nudix</i> gene, with several homologs in baculovirus, found by tblastn search. Comparison with the genome of the Colombian isolate SfGV VG008 resulted in SfGV being 1101 bp smaller and lacking a homologue of VG008 ORF084, which codes for Lef-7. However, we found that ORF051 shows remote homology to Lef-7 proteins. Moreover, analysis of ORF051 along with Lef-7 proteins coded by a group of noctuid specific GVs and NPVs indicated that Lef-7 proteins coded by these viruses include three F-box domains in contrast to the single one reported for AcMNPV Lef-7. SfGV ARG genome also contains a split photolyase as a distinct feature not found in VG008. BlastX analysis revealed that a complete photolyase is coded considering a putative frameshift in a poly-A tract, which resembles known slippery sequences involved in programmed ribosome frameshifting.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/104532
url http://sedici.unlp.edu.ar/handle/10915/104532
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://hdl.handle.net/11336/96973
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0202598
info:eu-repo/semantics/altIdentifier/issn/1932-6203
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0202598
info:eu-repo/semantics/altIdentifier/hdl/11336/96973
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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reponame_str SEDICI (UNLP)
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instname_str Universidad Nacional de La Plata
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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