Sensitivity of embryos and larvae of the freshwater prawn Macrobrachium borellii to the latest generation pesticide spirotetramat

Autores
Lavarías, Sabrina María Luisa; Arrighetti, Florencia; Landro, Sonia Maribel; Delevati Colpo, Karine
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to evaluate the effects of the last generation insecticide spirotetramat (STM) on embryos and larvae of the freshwater prawn Macrobrachium borellii. Both embryos and larvae were exposed to serial dilutions of STM to determine the LC₅₀ values. After 96-h of exposure, live larvae were fixed for histological analysis. In addition, ovigerous females were exposed to a sublethal concentration of STM (1.7 mg/L) for 96 h to evaluate the activity of the enzymes catalase, glutathione-S-transferase, and superoxide dismutase as well as the lipoperoxidation (LPO) and protein oxidation levels in embryos. The larvae showed a high sensitivity to STM evidenced by the LC₅₀-96 h value (0.011 mg/L). On the contrary, the embryos were highly resistant to STM exposure, and no lethal effect was observed in the treatments with high concentrations of this insecticide (LC₅₀-96 h > 150 mg/L). Among all the biochemical parameters evaluated in the embryos exposed to STM, only LPO showed a significant increase compared to controls. This was probably due to a restricted entry of the insecticide through the embryonic coat. Thus, a preliminary study of the structure and permeability of the embryonic coat was carried out in control embryos. The analysis by electron microscopy revealed that its structure is formed by four embryonic envelopes composed of multiple layers while the assay with a fluorescent probe revealed that the embryonic coat increases its permeability during development. STM caused significant histopathological alterations in the hepatopancreas and gills of larvae. This study showed that although the embryos of M. borellii could be protected by the embryonic coat, the larvae are very vulnerable to the STM toxicity. So, it is necessary to continue evaluating the effects of these new pesticides on non-target organisms, such as aquacultured species, to help predict their ecotoxicological risks derived from the increasing agricultural activity developed worldwide.
Instituto de Limnología "Dr. Raúl A. Ringuelet"
Materia
Ciencias Naturales
aquatic toxicology
histopathology
LC₅₀
oxidative stress
prawn
spirotetramat
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/155695

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spelling Sensitivity of embryos and larvae of the freshwater prawn Macrobrachium borellii to the latest generation pesticide spirotetramatLavarías, Sabrina María LuisaArrighetti, FlorenciaLandro, Sonia MaribelDelevati Colpo, KarineCiencias Naturalesaquatic toxicologyhistopathologyLC₅₀oxidative stressprawnspirotetramatThe aim of this study was to evaluate the effects of the last generation insecticide spirotetramat (STM) on embryos and larvae of the freshwater prawn Macrobrachium borellii. Both embryos and larvae were exposed to serial dilutions of STM to determine the LC₅₀ values. After 96-h of exposure, live larvae were fixed for histological analysis. In addition, ovigerous females were exposed to a sublethal concentration of STM (1.7 mg/L) for 96 h to evaluate the activity of the enzymes catalase, glutathione-S-transferase, and superoxide dismutase as well as the lipoperoxidation (LPO) and protein oxidation levels in embryos. The larvae showed a high sensitivity to STM evidenced by the LC₅₀-96 h value (0.011 mg/L). On the contrary, the embryos were highly resistant to STM exposure, and no lethal effect was observed in the treatments with high concentrations of this insecticide (LC₅₀-96 h > 150 mg/L). Among all the biochemical parameters evaluated in the embryos exposed to STM, only LPO showed a significant increase compared to controls. This was probably due to a restricted entry of the insecticide through the embryonic coat. Thus, a preliminary study of the structure and permeability of the embryonic coat was carried out in control embryos. The analysis by electron microscopy revealed that its structure is formed by four embryonic envelopes composed of multiple layers while the assay with a fluorescent probe revealed that the embryonic coat increases its permeability during development. STM caused significant histopathological alterations in the hepatopancreas and gills of larvae. This study showed that although the embryos of M. borellii could be protected by the embryonic coat, the larvae are very vulnerable to the STM toxicity. So, it is necessary to continue evaluating the effects of these new pesticides on non-target organisms, such as aquacultured species, to help predict their ecotoxicological risks derived from the increasing agricultural activity developed worldwide.Instituto de Limnología "Dr. Raúl A. Ringuelet"2022-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/155695enginfo:eu-repo/semantics/altIdentifier/issn/0147-6513info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2022.114257info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:12:25Zoai:sedici.unlp.edu.ar:10915/155695Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:12:25.925SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Sensitivity of embryos and larvae of the freshwater prawn Macrobrachium borellii to the latest generation pesticide spirotetramat
title Sensitivity of embryos and larvae of the freshwater prawn Macrobrachium borellii to the latest generation pesticide spirotetramat
spellingShingle Sensitivity of embryos and larvae of the freshwater prawn Macrobrachium borellii to the latest generation pesticide spirotetramat
Lavarías, Sabrina María Luisa
Ciencias Naturales
aquatic toxicology
histopathology
LC₅₀
oxidative stress
prawn
spirotetramat
title_short Sensitivity of embryos and larvae of the freshwater prawn Macrobrachium borellii to the latest generation pesticide spirotetramat
title_full Sensitivity of embryos and larvae of the freshwater prawn Macrobrachium borellii to the latest generation pesticide spirotetramat
title_fullStr Sensitivity of embryos and larvae of the freshwater prawn Macrobrachium borellii to the latest generation pesticide spirotetramat
title_full_unstemmed Sensitivity of embryos and larvae of the freshwater prawn Macrobrachium borellii to the latest generation pesticide spirotetramat
title_sort Sensitivity of embryos and larvae of the freshwater prawn Macrobrachium borellii to the latest generation pesticide spirotetramat
dc.creator.none.fl_str_mv Lavarías, Sabrina María Luisa
Arrighetti, Florencia
Landro, Sonia Maribel
Delevati Colpo, Karine
author Lavarías, Sabrina María Luisa
author_facet Lavarías, Sabrina María Luisa
Arrighetti, Florencia
Landro, Sonia Maribel
Delevati Colpo, Karine
author_role author
author2 Arrighetti, Florencia
Landro, Sonia Maribel
Delevati Colpo, Karine
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Naturales
aquatic toxicology
histopathology
LC₅₀
oxidative stress
prawn
spirotetramat
topic Ciencias Naturales
aquatic toxicology
histopathology
LC₅₀
oxidative stress
prawn
spirotetramat
dc.description.none.fl_txt_mv The aim of this study was to evaluate the effects of the last generation insecticide spirotetramat (STM) on embryos and larvae of the freshwater prawn Macrobrachium borellii. Both embryos and larvae were exposed to serial dilutions of STM to determine the LC₅₀ values. After 96-h of exposure, live larvae were fixed for histological analysis. In addition, ovigerous females were exposed to a sublethal concentration of STM (1.7 mg/L) for 96 h to evaluate the activity of the enzymes catalase, glutathione-S-transferase, and superoxide dismutase as well as the lipoperoxidation (LPO) and protein oxidation levels in embryos. The larvae showed a high sensitivity to STM evidenced by the LC₅₀-96 h value (0.011 mg/L). On the contrary, the embryos were highly resistant to STM exposure, and no lethal effect was observed in the treatments with high concentrations of this insecticide (LC₅₀-96 h > 150 mg/L). Among all the biochemical parameters evaluated in the embryos exposed to STM, only LPO showed a significant increase compared to controls. This was probably due to a restricted entry of the insecticide through the embryonic coat. Thus, a preliminary study of the structure and permeability of the embryonic coat was carried out in control embryos. The analysis by electron microscopy revealed that its structure is formed by four embryonic envelopes composed of multiple layers while the assay with a fluorescent probe revealed that the embryonic coat increases its permeability during development. STM caused significant histopathological alterations in the hepatopancreas and gills of larvae. This study showed that although the embryos of M. borellii could be protected by the embryonic coat, the larvae are very vulnerable to the STM toxicity. So, it is necessary to continue evaluating the effects of these new pesticides on non-target organisms, such as aquacultured species, to help predict their ecotoxicological risks derived from the increasing agricultural activity developed worldwide.
Instituto de Limnología "Dr. Raúl A. Ringuelet"
description The aim of this study was to evaluate the effects of the last generation insecticide spirotetramat (STM) on embryos and larvae of the freshwater prawn Macrobrachium borellii. Both embryos and larvae were exposed to serial dilutions of STM to determine the LC₅₀ values. After 96-h of exposure, live larvae were fixed for histological analysis. In addition, ovigerous females were exposed to a sublethal concentration of STM (1.7 mg/L) for 96 h to evaluate the activity of the enzymes catalase, glutathione-S-transferase, and superoxide dismutase as well as the lipoperoxidation (LPO) and protein oxidation levels in embryos. The larvae showed a high sensitivity to STM evidenced by the LC₅₀-96 h value (0.011 mg/L). On the contrary, the embryos were highly resistant to STM exposure, and no lethal effect was observed in the treatments with high concentrations of this insecticide (LC₅₀-96 h > 150 mg/L). Among all the biochemical parameters evaluated in the embryos exposed to STM, only LPO showed a significant increase compared to controls. This was probably due to a restricted entry of the insecticide through the embryonic coat. Thus, a preliminary study of the structure and permeability of the embryonic coat was carried out in control embryos. The analysis by electron microscopy revealed that its structure is formed by four embryonic envelopes composed of multiple layers while the assay with a fluorescent probe revealed that the embryonic coat increases its permeability during development. STM caused significant histopathological alterations in the hepatopancreas and gills of larvae. This study showed that although the embryos of M. borellii could be protected by the embryonic coat, the larvae are very vulnerable to the STM toxicity. So, it is necessary to continue evaluating the effects of these new pesticides on non-target organisms, such as aquacultured species, to help predict their ecotoxicological risks derived from the increasing agricultural activity developed worldwide.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
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info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2022.114257
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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