Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers
- Autores
- Henning, María Florencia; Sánchez, Susana A.; Bakás, Laura Susana
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Lipopolysaccharide (LPS) is an endotoxin released from the outer membrane of Gram-negative bacteria during infections. It have been reported that LPS may play a role in the outer membrane of bacteria similar to that of cholesterol in eukaryotic plasma membranes. In this article we compare the effect of introducing LPS or cholesterol in liposomes made of dipalmitoylphosphatidylcholine/dioleoylphosphatidylcholine on the solubilization process by Triton X-100. The results show that liposomes containing LPS or cholesterol are more resistant to solubilization by Triton X-100 than the binary phospholipid mixtures at 4 °C. The LPS distribution was analyzed on GUVs of DPPC:DOPC using FITC-LPS. Solid and liquid-crystalline domains were visualized labeling the GUVs with LAURDAN and GP images were acquired using a two-photon microscope. The images show a selective distribution of LPS in gel domains. Our results support the hypothesis that LPS could aggregate and concentrate selectively in biological membranes providing a mechanism to bring together several components of the LPS-sensing machinery.
Instituto de Investigaciones Bioquímicas de La Plata - Materia
-
Bioquímica
Membrane raft
Giant unilamellar vesicles
Two-photon microscopy
LAURDANFITC-LPS
Detergent solubilization
Triton X-100
LAURDAN generalized polarization
Cholesterol
Phase segregation - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/128273
Ver los metadatos del registro completo
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Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayersHenning, María FlorenciaSánchez, Susana A.Bakás, Laura SusanaBioquímicaMembrane raftGiant unilamellar vesiclesTwo-photon microscopyLAURDANFITC-LPSDetergent solubilizationTriton X-100LAURDAN generalized polarizationCholesterolPhase segregationLipopolysaccharide (LPS) is an endotoxin released from the outer membrane of Gram-negative bacteria during infections. It have been reported that LPS may play a role in the outer membrane of bacteria similar to that of cholesterol in eukaryotic plasma membranes. In this article we compare the effect of introducing LPS or cholesterol in liposomes made of dipalmitoylphosphatidylcholine/dioleoylphosphatidylcholine on the solubilization process by Triton X-100. The results show that liposomes containing LPS or cholesterol are more resistant to solubilization by Triton X-100 than the binary phospholipid mixtures at 4 °C. The LPS distribution was analyzed on GUVs of DPPC:DOPC using FITC-LPS. Solid and liquid-crystalline domains were visualized labeling the GUVs with LAURDAN and GP images were acquired using a two-photon microscope. The images show a selective distribution of LPS in gel domains. Our results support the hypothesis that LPS could aggregate and concentrate selectively in biological membranes providing a mechanism to bring together several components of the LPS-sensing machinery.Instituto de Investigaciones Bioquímicas de La Plata2009-03-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf22-26http://sedici.unlp.edu.ar/handle/10915/128273enginfo:eu-repo/semantics/altIdentifier/issn/1090-2104info:eu-repo/semantics/altIdentifier/issn/0006-291xinfo:eu-repo/semantics/altIdentifier/pmid/19324006info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbrc.2009.03.116info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:30:58Zoai:sedici.unlp.edu.ar:10915/128273Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:30:59.085SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers |
title |
Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers |
spellingShingle |
Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers Henning, María Florencia Bioquímica Membrane raft Giant unilamellar vesicles Two-photon microscopy LAURDANFITC-LPS Detergent solubilization Triton X-100 LAURDAN generalized polarization Cholesterol Phase segregation |
title_short |
Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers |
title_full |
Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers |
title_fullStr |
Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers |
title_full_unstemmed |
Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers |
title_sort |
Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers |
dc.creator.none.fl_str_mv |
Henning, María Florencia Sánchez, Susana A. Bakás, Laura Susana |
author |
Henning, María Florencia |
author_facet |
Henning, María Florencia Sánchez, Susana A. Bakás, Laura Susana |
author_role |
author |
author2 |
Sánchez, Susana A. Bakás, Laura Susana |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Bioquímica Membrane raft Giant unilamellar vesicles Two-photon microscopy LAURDANFITC-LPS Detergent solubilization Triton X-100 LAURDAN generalized polarization Cholesterol Phase segregation |
topic |
Bioquímica Membrane raft Giant unilamellar vesicles Two-photon microscopy LAURDANFITC-LPS Detergent solubilization Triton X-100 LAURDAN generalized polarization Cholesterol Phase segregation |
dc.description.none.fl_txt_mv |
Lipopolysaccharide (LPS) is an endotoxin released from the outer membrane of Gram-negative bacteria during infections. It have been reported that LPS may play a role in the outer membrane of bacteria similar to that of cholesterol in eukaryotic plasma membranes. In this article we compare the effect of introducing LPS or cholesterol in liposomes made of dipalmitoylphosphatidylcholine/dioleoylphosphatidylcholine on the solubilization process by Triton X-100. The results show that liposomes containing LPS or cholesterol are more resistant to solubilization by Triton X-100 than the binary phospholipid mixtures at 4 °C. The LPS distribution was analyzed on GUVs of DPPC:DOPC using FITC-LPS. Solid and liquid-crystalline domains were visualized labeling the GUVs with LAURDAN and GP images were acquired using a two-photon microscope. The images show a selective distribution of LPS in gel domains. Our results support the hypothesis that LPS could aggregate and concentrate selectively in biological membranes providing a mechanism to bring together several components of the LPS-sensing machinery. Instituto de Investigaciones Bioquímicas de La Plata |
description |
Lipopolysaccharide (LPS) is an endotoxin released from the outer membrane of Gram-negative bacteria during infections. It have been reported that LPS may play a role in the outer membrane of bacteria similar to that of cholesterol in eukaryotic plasma membranes. In this article we compare the effect of introducing LPS or cholesterol in liposomes made of dipalmitoylphosphatidylcholine/dioleoylphosphatidylcholine on the solubilization process by Triton X-100. The results show that liposomes containing LPS or cholesterol are more resistant to solubilization by Triton X-100 than the binary phospholipid mixtures at 4 °C. The LPS distribution was analyzed on GUVs of DPPC:DOPC using FITC-LPS. Solid and liquid-crystalline domains were visualized labeling the GUVs with LAURDAN and GP images were acquired using a two-photon microscope. The images show a selective distribution of LPS in gel domains. Our results support the hypothesis that LPS could aggregate and concentrate selectively in biological membranes providing a mechanism to bring together several components of the LPS-sensing machinery. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-03-24 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/128273 |
url |
http://sedici.unlp.edu.ar/handle/10915/128273 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/1090-2104 info:eu-repo/semantics/altIdentifier/issn/0006-291x info:eu-repo/semantics/altIdentifier/pmid/19324006 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbrc.2009.03.116 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf 22-26 |
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SEDICI (UNLP) - Universidad Nacional de La Plata |
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