Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers

Autores
Henning, María Florencia; Sánchez, Susana A.; Bakás, Laura Susana
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lipopolysaccharide (LPS) is an endotoxin released from the outer membrane of Gram-negative bacteria during infections. It have been reported that LPS may play a role in the outer membrane of bacteria similar to that of cholesterol in eukaryotic plasma membranes. In this article we compare the effect of introducing LPS or cholesterol in liposomes made of dipalmitoylphosphatidylcholine/dioleoylphosphatidylcholine on the solubilization process by Triton X-100. The results show that liposomes containing LPS or cholesterol are more resistant to solubilization by Triton X-100 than the binary phospholipid mixtures at 4 °C. The LPS distribution was analyzed on GUVs of DPPC:DOPC using FITC-LPS. Solid and liquid-crystalline domains were visualized labeling the GUVs with LAURDAN and GP images were acquired using a two-photon microscope. The images show a selective distribution of LPS in gel domains. Our results support the hypothesis that LPS could aggregate and concentrate selectively in biological membranes providing a mechanism to bring together several components of the LPS-sensing machinery.
Instituto de Investigaciones Bioquímicas de La Plata
Materia
Bioquímica
Membrane raft
Giant unilamellar vesicles
Two-photon microscopy
LAURDANFITC-LPS
Detergent solubilization
Triton X-100
LAURDAN generalized polarization
Cholesterol
Phase segregation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/128273

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oai_identifier_str oai:sedici.unlp.edu.ar:10915/128273
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network_name_str SEDICI (UNLP)
spelling Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayersHenning, María FlorenciaSánchez, Susana A.Bakás, Laura SusanaBioquímicaMembrane raftGiant unilamellar vesiclesTwo-photon microscopyLAURDANFITC-LPSDetergent solubilizationTriton X-100LAURDAN generalized polarizationCholesterolPhase segregationLipopolysaccharide (LPS) is an endotoxin released from the outer membrane of Gram-negative bacteria during infections. It have been reported that LPS may play a role in the outer membrane of bacteria similar to that of cholesterol in eukaryotic plasma membranes. In this article we compare the effect of introducing LPS or cholesterol in liposomes made of dipalmitoylphosphatidylcholine/dioleoylphosphatidylcholine on the solubilization process by Triton X-100. The results show that liposomes containing LPS or cholesterol are more resistant to solubilization by Triton X-100 than the binary phospholipid mixtures at 4 °C. The LPS distribution was analyzed on GUVs of DPPC:DOPC using FITC-LPS. Solid and liquid-crystalline domains were visualized labeling the GUVs with LAURDAN and GP images were acquired using a two-photon microscope. The images show a selective distribution of LPS in gel domains. Our results support the hypothesis that LPS could aggregate and concentrate selectively in biological membranes providing a mechanism to bring together several components of the LPS-sensing machinery.Instituto de Investigaciones Bioquímicas de La Plata2009-03-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf22-26http://sedici.unlp.edu.ar/handle/10915/128273enginfo:eu-repo/semantics/altIdentifier/issn/1090-2104info:eu-repo/semantics/altIdentifier/issn/0006-291xinfo:eu-repo/semantics/altIdentifier/pmid/19324006info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbrc.2009.03.116info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:30:58Zoai:sedici.unlp.edu.ar:10915/128273Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:30:59.085SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers
title Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers
spellingShingle Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers
Henning, María Florencia
Bioquímica
Membrane raft
Giant unilamellar vesicles
Two-photon microscopy
LAURDANFITC-LPS
Detergent solubilization
Triton X-100
LAURDAN generalized polarization
Cholesterol
Phase segregation
title_short Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers
title_full Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers
title_fullStr Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers
title_full_unstemmed Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers
title_sort Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers
dc.creator.none.fl_str_mv Henning, María Florencia
Sánchez, Susana A.
Bakás, Laura Susana
author Henning, María Florencia
author_facet Henning, María Florencia
Sánchez, Susana A.
Bakás, Laura Susana
author_role author
author2 Sánchez, Susana A.
Bakás, Laura Susana
author2_role author
author
dc.subject.none.fl_str_mv Bioquímica
Membrane raft
Giant unilamellar vesicles
Two-photon microscopy
LAURDANFITC-LPS
Detergent solubilization
Triton X-100
LAURDAN generalized polarization
Cholesterol
Phase segregation
topic Bioquímica
Membrane raft
Giant unilamellar vesicles
Two-photon microscopy
LAURDANFITC-LPS
Detergent solubilization
Triton X-100
LAURDAN generalized polarization
Cholesterol
Phase segregation
dc.description.none.fl_txt_mv Lipopolysaccharide (LPS) is an endotoxin released from the outer membrane of Gram-negative bacteria during infections. It have been reported that LPS may play a role in the outer membrane of bacteria similar to that of cholesterol in eukaryotic plasma membranes. In this article we compare the effect of introducing LPS or cholesterol in liposomes made of dipalmitoylphosphatidylcholine/dioleoylphosphatidylcholine on the solubilization process by Triton X-100. The results show that liposomes containing LPS or cholesterol are more resistant to solubilization by Triton X-100 than the binary phospholipid mixtures at 4 °C. The LPS distribution was analyzed on GUVs of DPPC:DOPC using FITC-LPS. Solid and liquid-crystalline domains were visualized labeling the GUVs with LAURDAN and GP images were acquired using a two-photon microscope. The images show a selective distribution of LPS in gel domains. Our results support the hypothesis that LPS could aggregate and concentrate selectively in biological membranes providing a mechanism to bring together several components of the LPS-sensing machinery.
Instituto de Investigaciones Bioquímicas de La Plata
description Lipopolysaccharide (LPS) is an endotoxin released from the outer membrane of Gram-negative bacteria during infections. It have been reported that LPS may play a role in the outer membrane of bacteria similar to that of cholesterol in eukaryotic plasma membranes. In this article we compare the effect of introducing LPS or cholesterol in liposomes made of dipalmitoylphosphatidylcholine/dioleoylphosphatidylcholine on the solubilization process by Triton X-100. The results show that liposomes containing LPS or cholesterol are more resistant to solubilization by Triton X-100 than the binary phospholipid mixtures at 4 °C. The LPS distribution was analyzed on GUVs of DPPC:DOPC using FITC-LPS. Solid and liquid-crystalline domains were visualized labeling the GUVs with LAURDAN and GP images were acquired using a two-photon microscope. The images show a selective distribution of LPS in gel domains. Our results support the hypothesis that LPS could aggregate and concentrate selectively in biological membranes providing a mechanism to bring together several components of the LPS-sensing machinery.
publishDate 2009
dc.date.none.fl_str_mv 2009-03-24
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/128273
url http://sedici.unlp.edu.ar/handle/10915/128273
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1090-2104
info:eu-repo/semantics/altIdentifier/issn/0006-291x
info:eu-repo/semantics/altIdentifier/pmid/19324006
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbrc.2009.03.116
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
22-26
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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