Efecto de OPN3 y MC4R en las corrientes mediadas por Kir.1

Autores
Pinto, Victoria
Año de publicación
2022
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Encephalopsin (OPN3) is part of the opsin family of light-sensitive G-protein coupled receptors. OPN3 was discovered in deep brain regions, specifically the arcuate nucleus and the paraventricular nucleus of the hypothalamus, however there have been limited research in the functionality of OPN3. Oancea lab has discovered that OPN3 functions in a light independent manner and signals through Gai to negatively regulate the melanogenic Gαs-coupled MC1R in human epidermal melanocytes. Neuronal melanocortin receptors (MC3R and MC4R) are found in the arcuate nucleus and paraventricular nucleus of the hypothalamus and play non-redundant roles in mediating energy balance. Both MC3R and MC4R share similar structural identities to MC1R and have been found to couple to Gαs. Additionally, Cone and his colleagues found that MC4R couples to Kir7.1 in hypothalamic neurons independent of G-protein activity. Previous data in our lab has revealed that OPN3 colocalizes and forms a physical complex with MC4R. The goal of this study is to test the hypothesis that OPN3 functions to negatively modulate MC4R-mediated signaling. More specifically, we overexpress OPN3 and MC4R in HEK293 cells together with Kir7.1 and measured the potassium current at diferent potentials. We only observed a significant amount of current in cells coexpressing OPN3 and this effect seems to be occluded by MC4R addition. More experiments are required to fully understand the signaling cascade involved and what is the effect of MC4R agonists.
Carrera: Doctorado en Ciencias Exactas Tipo de beca: Beca Doctoral Año de inicio de beca: 2022 Año de finalización de beca: 2027 Organismo: AGENCIA Apellido, Nombre del Director/a/e: Raingo, Jesica Lugar de desarrollo: Instituto Multidisciplinario de Biología Celular (IMBICE) Tipo de investigación: Básica
Facultad de Ciencias Exactas
Materia
Neurociencia
Patch Clamp
Corrientes
Receptores
Patch Clamp
Currents
Receptors
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/145690
Acceder
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spelling Efecto de OPN3 y MC4R en las corrientes mediadas por Kir.1Effect of OPN3 and MC4R on Kir7.1 mediated currents.Pinto, VictoriaNeurocienciaPatch ClampCorrientesReceptoresPatch ClampCurrentsReceptorsEncephalopsin (OPN3) is part of the opsin family of light-sensitive G-protein coupled receptors. OPN3 was discovered in deep brain regions, specifically the arcuate nucleus and the paraventricular nucleus of the hypothalamus, however there have been limited research in the functionality of OPN3. Oancea lab has discovered that OPN3 functions in a light independent manner and signals through Gai to negatively regulate the melanogenic Gαs-coupled MC1R in human epidermal melanocytes. Neuronal melanocortin receptors (MC3R and MC4R) are found in the arcuate nucleus and paraventricular nucleus of the hypothalamus and play non-redundant roles in mediating energy balance. Both MC3R and MC4R share similar structural identities to MC1R and have been found to couple to Gαs. Additionally, Cone and his colleagues found that MC4R couples to Kir7.1 in hypothalamic neurons independent of G-protein activity. Previous data in our lab has revealed that OPN3 colocalizes and forms a physical complex with MC4R. The goal of this study is to test the hypothesis that OPN3 functions to negatively modulate MC4R-mediated signaling. More specifically, we overexpress OPN3 and MC4R in HEK293 cells together with Kir7.1 and measured the potassium current at diferent potentials. We only observed a significant amount of current in cells coexpressing OPN3 and this effect seems to be occluded by MC4R addition. More experiments are required to fully understand the signaling cascade involved and what is the effect of MC4R agonists.Carrera: Doctorado en Ciencias Exactas Tipo de beca: Beca Doctoral Año de inicio de beca: 2022 Año de finalización de beca: 2027 Organismo: AGENCIA Apellido, Nombre del Director/a/e: Raingo, Jesica Lugar de desarrollo: Instituto Multidisciplinario de Biología Celular (IMBICE) Tipo de investigación: BásicaFacultad de Ciencias Exactas2022-11-23info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionObjeto de conferenciahttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/145690spainfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:18:00Zoai:sedici.unlp.edu.ar:10915/145690Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:18:01.233SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Efecto de OPN3 y MC4R en las corrientes mediadas por Kir.1
Effect of OPN3 and MC4R on Kir7.1 mediated currents.
title Efecto de OPN3 y MC4R en las corrientes mediadas por Kir.1
spellingShingle Efecto de OPN3 y MC4R en las corrientes mediadas por Kir.1
Pinto, Victoria
Neurociencia
Patch Clamp
Corrientes
Receptores
Patch Clamp
Currents
Receptors
title_short Efecto de OPN3 y MC4R en las corrientes mediadas por Kir.1
title_full Efecto de OPN3 y MC4R en las corrientes mediadas por Kir.1
title_fullStr Efecto de OPN3 y MC4R en las corrientes mediadas por Kir.1
title_full_unstemmed Efecto de OPN3 y MC4R en las corrientes mediadas por Kir.1
title_sort Efecto de OPN3 y MC4R en las corrientes mediadas por Kir.1
dc.creator.none.fl_str_mv Pinto, Victoria
author Pinto, Victoria
author_facet Pinto, Victoria
author_role author
dc.subject.none.fl_str_mv Neurociencia
Patch Clamp
Corrientes
Receptores
Patch Clamp
Currents
Receptors
topic Neurociencia
Patch Clamp
Corrientes
Receptores
Patch Clamp
Currents
Receptors
dc.description.none.fl_txt_mv Encephalopsin (OPN3) is part of the opsin family of light-sensitive G-protein coupled receptors. OPN3 was discovered in deep brain regions, specifically the arcuate nucleus and the paraventricular nucleus of the hypothalamus, however there have been limited research in the functionality of OPN3. Oancea lab has discovered that OPN3 functions in a light independent manner and signals through Gai to negatively regulate the melanogenic Gαs-coupled MC1R in human epidermal melanocytes. Neuronal melanocortin receptors (MC3R and MC4R) are found in the arcuate nucleus and paraventricular nucleus of the hypothalamus and play non-redundant roles in mediating energy balance. Both MC3R and MC4R share similar structural identities to MC1R and have been found to couple to Gαs. Additionally, Cone and his colleagues found that MC4R couples to Kir7.1 in hypothalamic neurons independent of G-protein activity. Previous data in our lab has revealed that OPN3 colocalizes and forms a physical complex with MC4R. The goal of this study is to test the hypothesis that OPN3 functions to negatively modulate MC4R-mediated signaling. More specifically, we overexpress OPN3 and MC4R in HEK293 cells together with Kir7.1 and measured the potassium current at diferent potentials. We only observed a significant amount of current in cells coexpressing OPN3 and this effect seems to be occluded by MC4R addition. More experiments are required to fully understand the signaling cascade involved and what is the effect of MC4R agonists.
Carrera: Doctorado en Ciencias Exactas Tipo de beca: Beca Doctoral Año de inicio de beca: 2022 Año de finalización de beca: 2027 Organismo: AGENCIA Apellido, Nombre del Director/a/e: Raingo, Jesica Lugar de desarrollo: Instituto Multidisciplinario de Biología Celular (IMBICE) Tipo de investigación: Básica
Facultad de Ciencias Exactas
description Encephalopsin (OPN3) is part of the opsin family of light-sensitive G-protein coupled receptors. OPN3 was discovered in deep brain regions, specifically the arcuate nucleus and the paraventricular nucleus of the hypothalamus, however there have been limited research in the functionality of OPN3. Oancea lab has discovered that OPN3 functions in a light independent manner and signals through Gai to negatively regulate the melanogenic Gαs-coupled MC1R in human epidermal melanocytes. Neuronal melanocortin receptors (MC3R and MC4R) are found in the arcuate nucleus and paraventricular nucleus of the hypothalamus and play non-redundant roles in mediating energy balance. Both MC3R and MC4R share similar structural identities to MC1R and have been found to couple to Gαs. Additionally, Cone and his colleagues found that MC4R couples to Kir7.1 in hypothalamic neurons independent of G-protein activity. Previous data in our lab has revealed that OPN3 colocalizes and forms a physical complex with MC4R. The goal of this study is to test the hypothesis that OPN3 functions to negatively modulate MC4R-mediated signaling. More specifically, we overexpress OPN3 and MC4R in HEK293 cells together with Kir7.1 and measured the potassium current at diferent potentials. We only observed a significant amount of current in cells coexpressing OPN3 and this effect seems to be occluded by MC4R addition. More experiments are required to fully understand the signaling cascade involved and what is the effect of MC4R agonists.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-23
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/publishedVersion
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http://purl.org/coar/resource_type/c_5794
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url http://sedici.unlp.edu.ar/handle/10915/145690
dc.language.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
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