Genetic diversity of Burkholderia contaminans isolates from cystic fibrosis patients in Argentina

Autores
Martina, Pablo Francisco; Bettiol, Marisa; Vescina, Cecilia; Montanaro, Patricia; Mannino, María Constanza; Prieto, Claudia Inés; Vay, Carlos; Naumann, Dieter; Schmitt, Juergen; Yantorno, Osvaldo Miguel; Lagares, Antonio; Bosch, María Alejandra
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A total of 120 Burkholderia cepacia complex isolates collected during 2004-2010 from 66 patients in two cystic fibrosis reference centers in Argentina were analyzed. Burkholderia contaminans was the species most frequently recovered (57.6%), followed by Burkholderia cenocepacia (15%), a species distribution not reported so far. The recA-PCR-based techniques applied to the B. contaminans isolates revealed that 85% of the population carried the recA-ST-71 allele. Our results showed the utility of BOXPCR genotyping in analyzing B. contaminans diversity. This approach allowed us to address clonal transmission during an outbreak and the genetic changes occurring in infecting bacteria over the course of chronic infection.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Instituto de Biotecnologia y Biologia Molecular
Materia
Biología
Burkholderia
cystic fibrosis
BOXPCR genotyping
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/84971
Acceder
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network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Genetic diversity of Burkholderia contaminans isolates from cystic fibrosis patients in ArgentinaMartina, Pablo FranciscoBettiol, MarisaVescina, CeciliaMontanaro, PatriciaMannino, María ConstanzaPrieto, Claudia InésVay, CarlosNaumann, DieterSchmitt, JuergenYantorno, Osvaldo MiguelLagares, AntonioBosch, María AlejandraBiologíaBurkholderiacystic fibrosisBOXPCR genotypingA total of 120 Burkholderia cepacia complex isolates collected during 2004-2010 from 66 patients in two cystic fibrosis reference centers in Argentina were analyzed. Burkholderia contaminans was the species most frequently recovered (57.6%), followed by Burkholderia cenocepacia (15%), a species distribution not reported so far. The recA-PCR-based techniques applied to the B. contaminans isolates revealed that 85% of the population carried the recA-ST-71 allele. Our results showed the utility of BOXPCR genotyping in analyzing B. contaminans diversity. This approach allowed us to address clonal transmission during an outbreak and the genetic changes occurring in infecting bacteria over the course of chronic infection.Centro de Investigación y Desarrollo en Fermentaciones IndustrialesInstituto de Biotecnologia y Biologia Molecular2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf339-344http://sedici.unlp.edu.ar/handle/10915/84971enginfo:eu-repo/semantics/altIdentifier/issn/0095-1137info:eu-repo/semantics/altIdentifier/doi/10.1128/JCM.02500-12info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:48:50Zoai:sedici.unlp.edu.ar:10915/84971Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:48:51.064SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Genetic diversity of Burkholderia contaminans isolates from cystic fibrosis patients in Argentina
title Genetic diversity of Burkholderia contaminans isolates from cystic fibrosis patients in Argentina
spellingShingle Genetic diversity of Burkholderia contaminans isolates from cystic fibrosis patients in Argentina
Martina, Pablo Francisco
Biología
Burkholderia
cystic fibrosis
BOXPCR genotyping
title_short Genetic diversity of Burkholderia contaminans isolates from cystic fibrosis patients in Argentina
title_full Genetic diversity of Burkholderia contaminans isolates from cystic fibrosis patients in Argentina
title_fullStr Genetic diversity of Burkholderia contaminans isolates from cystic fibrosis patients in Argentina
title_full_unstemmed Genetic diversity of Burkholderia contaminans isolates from cystic fibrosis patients in Argentina
title_sort Genetic diversity of Burkholderia contaminans isolates from cystic fibrosis patients in Argentina
dc.creator.none.fl_str_mv Martina, Pablo Francisco
Bettiol, Marisa
Vescina, Cecilia
Montanaro, Patricia
Mannino, María Constanza
Prieto, Claudia Inés
Vay, Carlos
Naumann, Dieter
Schmitt, Juergen
Yantorno, Osvaldo Miguel
Lagares, Antonio
Bosch, María Alejandra
author Martina, Pablo Francisco
author_facet Martina, Pablo Francisco
Bettiol, Marisa
Vescina, Cecilia
Montanaro, Patricia
Mannino, María Constanza
Prieto, Claudia Inés
Vay, Carlos
Naumann, Dieter
Schmitt, Juergen
Yantorno, Osvaldo Miguel
Lagares, Antonio
Bosch, María Alejandra
author_role author
author2 Bettiol, Marisa
Vescina, Cecilia
Montanaro, Patricia
Mannino, María Constanza
Prieto, Claudia Inés
Vay, Carlos
Naumann, Dieter
Schmitt, Juergen
Yantorno, Osvaldo Miguel
Lagares, Antonio
Bosch, María Alejandra
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Burkholderia
cystic fibrosis
BOXPCR genotyping
topic Biología
Burkholderia
cystic fibrosis
BOXPCR genotyping
dc.description.none.fl_txt_mv A total of 120 Burkholderia cepacia complex isolates collected during 2004-2010 from 66 patients in two cystic fibrosis reference centers in Argentina were analyzed. Burkholderia contaminans was the species most frequently recovered (57.6%), followed by Burkholderia cenocepacia (15%), a species distribution not reported so far. The recA-PCR-based techniques applied to the B. contaminans isolates revealed that 85% of the population carried the recA-ST-71 allele. Our results showed the utility of BOXPCR genotyping in analyzing B. contaminans diversity. This approach allowed us to address clonal transmission during an outbreak and the genetic changes occurring in infecting bacteria over the course of chronic infection.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Instituto de Biotecnologia y Biologia Molecular
description A total of 120 Burkholderia cepacia complex isolates collected during 2004-2010 from 66 patients in two cystic fibrosis reference centers in Argentina were analyzed. Burkholderia contaminans was the species most frequently recovered (57.6%), followed by Burkholderia cenocepacia (15%), a species distribution not reported so far. The recA-PCR-based techniques applied to the B. contaminans isolates revealed that 85% of the population carried the recA-ST-71 allele. Our results showed the utility of BOXPCR genotyping in analyzing B. contaminans diversity. This approach allowed us to address clonal transmission during an outbreak and the genetic changes occurring in infecting bacteria over the course of chronic infection.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/84971
url http://sedici.unlp.edu.ar/handle/10915/84971
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0095-1137
info:eu-repo/semantics/altIdentifier/doi/10.1128/JCM.02500-12
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
339-344
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.13397

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