Intersex and liver alterations induced by long‐term sublethal exposure to 17α‐ethinylestradiol in adult male <i>Cnesterodon decemmaculatus</i> (Pisces: Poeciliidae)
- Autores
- Young, Brian Jonathan; López, Gabriela Carina; Cristos, Diego Sebastián; Crespo, Diana Cristina; Somoza, Gustavo Manuel; Carriquiriborde, Pedro
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The aim of the present study was to assess the responses of the gonopodium morphology and the gonadal and liver histology of adult male Cnesterodon decemmaculatus to sublethal long-term exposure concentrations of 17a-ethinylestradiol (EE2). Two experiments were conducted exposing the fish to waterborne concentrations of EE2 ranging from 20 ng/L to 200 ng/L for 8 wk, 12 wk, and 16 wk. Intersex gonads were observed after 8 wk and 16 wk in fish exposed to 200 ng EE2/L and 100 ng EE2/L, respectively. Oocytes’ development from testis germ cells and replacement of the efferent duct periodic acid-Schiff–positive secretion surrounding spermatozeugmata by parenchymal tissue and duct structure alterations were the major observed changes in the gonads. In contrast, no response was observed in the gonopodium morphology. Liver histology was also altered, showing increasing steatosis, single-cell necrosis to generalized necrosis, and disruption of acinar organization from 100 ng EE2/L to 200 ng EE2/L. In summary, the present results showed that although EE2 was not able to alter the morphology of a developed gonopodium, it was capable of inducing development of testicular oocytes in adult male C. decemmaculatus at environmentally relevant concentrations. Thus, externally normal but intersex C. decemmaculatus males would be expected in the wastewater-receiving streams that the species inhabits. According to the literature, the present study would be the first indicating estrogen-induced intersex in adult male poeciliid.
Centro de Investigaciones del Medioambiente - Materia
-
Química
Ciencias Exactas
Ecotoxicology
Estrogenic compounds
Testis-ova
Hepatotoxicity
Cnesterodon decemmaculatus - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/104815
Ver los metadatos del registro completo
id |
SEDICI_303ef540971c7c54164db75163c19e6e |
---|---|
oai_identifier_str |
oai:sedici.unlp.edu.ar:10915/104815 |
network_acronym_str |
SEDICI |
repository_id_str |
1329 |
network_name_str |
SEDICI (UNLP) |
spelling |
Intersex and liver alterations induced by long‐term sublethal exposure to 17α‐ethinylestradiol in adult male <i>Cnesterodon decemmaculatus</i> (Pisces: Poeciliidae)Young, Brian JonathanLópez, Gabriela CarinaCristos, Diego SebastiánCrespo, Diana CristinaSomoza, Gustavo ManuelCarriquiriborde, PedroQuímicaCiencias ExactasEcotoxicologyEstrogenic compoundsTestis-ovaHepatotoxicityCnesterodon decemmaculatusThe aim of the present study was to assess the responses of the gonopodium morphology and the gonadal and liver histology of adult male <i>Cnesterodon decemmaculatus</i> to sublethal long-term exposure concentrations of 17a-ethinylestradiol (EE2). Two experiments were conducted exposing the fish to waterborne concentrations of EE2 ranging from 20 ng/L to 200 ng/L for 8 wk, 12 wk, and 16 wk. Intersex gonads were observed after 8 wk and 16 wk in fish exposed to 200 ng EE2/L and 100 ng EE2/L, respectively. Oocytes’ development from testis germ cells and replacement of the efferent duct periodic acid-Schiff–positive secretion surrounding spermatozeugmata by parenchymal tissue and duct structure alterations were the major observed changes in the gonads. In contrast, no response was observed in the gonopodium morphology. Liver histology was also altered, showing increasing steatosis, single-cell necrosis to generalized necrosis, and disruption of acinar organization from 100 ng EE2/L to 200 ng EE2/L. In summary, the present results showed that although EE2 was not able to alter the morphology of a developed gonopodium, it was capable of inducing development of testicular oocytes in adult male <i>C. decemmaculatus</i> at environmentally relevant concentrations. Thus, externally normal but intersex <i>C. decemmaculatus</i> males would be expected in the wastewater-receiving streams that the species inhabits. According to the literature, the present study would be the first indicating estrogen-induced intersex in adult male poeciliid.Centro de Investigaciones del Medioambiente2016-07-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1738–1745http://sedici.unlp.edu.ar/handle/10915/104815enginfo:eu-repo/semantics/altIdentifier/issn/1552-8618info:eu-repo/semantics/altIdentifier/doi/10.1002/etc.3547info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:14:49Zoai:sedici.unlp.edu.ar:10915/104815Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:14:49.924SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Intersex and liver alterations induced by long‐term sublethal exposure to 17α‐ethinylestradiol in adult male <i>Cnesterodon decemmaculatus</i> (Pisces: Poeciliidae) |
title |
Intersex and liver alterations induced by long‐term sublethal exposure to 17α‐ethinylestradiol in adult male <i>Cnesterodon decemmaculatus</i> (Pisces: Poeciliidae) |
spellingShingle |
Intersex and liver alterations induced by long‐term sublethal exposure to 17α‐ethinylestradiol in adult male <i>Cnesterodon decemmaculatus</i> (Pisces: Poeciliidae) Young, Brian Jonathan Química Ciencias Exactas Ecotoxicology Estrogenic compounds Testis-ova Hepatotoxicity Cnesterodon decemmaculatus |
title_short |
Intersex and liver alterations induced by long‐term sublethal exposure to 17α‐ethinylestradiol in adult male <i>Cnesterodon decemmaculatus</i> (Pisces: Poeciliidae) |
title_full |
Intersex and liver alterations induced by long‐term sublethal exposure to 17α‐ethinylestradiol in adult male <i>Cnesterodon decemmaculatus</i> (Pisces: Poeciliidae) |
title_fullStr |
Intersex and liver alterations induced by long‐term sublethal exposure to 17α‐ethinylestradiol in adult male <i>Cnesterodon decemmaculatus</i> (Pisces: Poeciliidae) |
title_full_unstemmed |
Intersex and liver alterations induced by long‐term sublethal exposure to 17α‐ethinylestradiol in adult male <i>Cnesterodon decemmaculatus</i> (Pisces: Poeciliidae) |
title_sort |
Intersex and liver alterations induced by long‐term sublethal exposure to 17α‐ethinylestradiol in adult male <i>Cnesterodon decemmaculatus</i> (Pisces: Poeciliidae) |
dc.creator.none.fl_str_mv |
Young, Brian Jonathan López, Gabriela Carina Cristos, Diego Sebastián Crespo, Diana Cristina Somoza, Gustavo Manuel Carriquiriborde, Pedro |
author |
Young, Brian Jonathan |
author_facet |
Young, Brian Jonathan López, Gabriela Carina Cristos, Diego Sebastián Crespo, Diana Cristina Somoza, Gustavo Manuel Carriquiriborde, Pedro |
author_role |
author |
author2 |
López, Gabriela Carina Cristos, Diego Sebastián Crespo, Diana Cristina Somoza, Gustavo Manuel Carriquiriborde, Pedro |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Química Ciencias Exactas Ecotoxicology Estrogenic compounds Testis-ova Hepatotoxicity Cnesterodon decemmaculatus |
topic |
Química Ciencias Exactas Ecotoxicology Estrogenic compounds Testis-ova Hepatotoxicity Cnesterodon decemmaculatus |
dc.description.none.fl_txt_mv |
The aim of the present study was to assess the responses of the gonopodium morphology and the gonadal and liver histology of adult male <i>Cnesterodon decemmaculatus</i> to sublethal long-term exposure concentrations of 17a-ethinylestradiol (EE2). Two experiments were conducted exposing the fish to waterborne concentrations of EE2 ranging from 20 ng/L to 200 ng/L for 8 wk, 12 wk, and 16 wk. Intersex gonads were observed after 8 wk and 16 wk in fish exposed to 200 ng EE2/L and 100 ng EE2/L, respectively. Oocytes’ development from testis germ cells and replacement of the efferent duct periodic acid-Schiff–positive secretion surrounding spermatozeugmata by parenchymal tissue and duct structure alterations were the major observed changes in the gonads. In contrast, no response was observed in the gonopodium morphology. Liver histology was also altered, showing increasing steatosis, single-cell necrosis to generalized necrosis, and disruption of acinar organization from 100 ng EE2/L to 200 ng EE2/L. In summary, the present results showed that although EE2 was not able to alter the morphology of a developed gonopodium, it was capable of inducing development of testicular oocytes in adult male <i>C. decemmaculatus</i> at environmentally relevant concentrations. Thus, externally normal but intersex <i>C. decemmaculatus</i> males would be expected in the wastewater-receiving streams that the species inhabits. According to the literature, the present study would be the first indicating estrogen-induced intersex in adult male poeciliid. Centro de Investigaciones del Medioambiente |
description |
The aim of the present study was to assess the responses of the gonopodium morphology and the gonadal and liver histology of adult male <i>Cnesterodon decemmaculatus</i> to sublethal long-term exposure concentrations of 17a-ethinylestradiol (EE2). Two experiments were conducted exposing the fish to waterborne concentrations of EE2 ranging from 20 ng/L to 200 ng/L for 8 wk, 12 wk, and 16 wk. Intersex gonads were observed after 8 wk and 16 wk in fish exposed to 200 ng EE2/L and 100 ng EE2/L, respectively. Oocytes’ development from testis germ cells and replacement of the efferent duct periodic acid-Schiff–positive secretion surrounding spermatozeugmata by parenchymal tissue and duct structure alterations were the major observed changes in the gonads. In contrast, no response was observed in the gonopodium morphology. Liver histology was also altered, showing increasing steatosis, single-cell necrosis to generalized necrosis, and disruption of acinar organization from 100 ng EE2/L to 200 ng EE2/L. In summary, the present results showed that although EE2 was not able to alter the morphology of a developed gonopodium, it was capable of inducing development of testicular oocytes in adult male <i>C. decemmaculatus</i> at environmentally relevant concentrations. Thus, externally normal but intersex <i>C. decemmaculatus</i> males would be expected in the wastewater-receiving streams that the species inhabits. According to the literature, the present study would be the first indicating estrogen-induced intersex in adult male poeciliid. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/104815 |
url |
http://sedici.unlp.edu.ar/handle/10915/104815 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/1552-8618 info:eu-repo/semantics/altIdentifier/doi/10.1002/etc.3547 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf 1738–1745 |
dc.source.none.fl_str_mv |
reponame:SEDICI (UNLP) instname:Universidad Nacional de La Plata instacron:UNLP |
reponame_str |
SEDICI (UNLP) |
collection |
SEDICI (UNLP) |
instname_str |
Universidad Nacional de La Plata |
instacron_str |
UNLP |
institution |
UNLP |
repository.name.fl_str_mv |
SEDICI (UNLP) - Universidad Nacional de La Plata |
repository.mail.fl_str_mv |
alira@sedici.unlp.edu.ar |
_version_ |
1846064206192836608 |
score |
13.22299 |