Intersex and liver alterations induced by long‐term sublethal exposure to 17α‐ethinylestradiol in adult male <i>Cnesterodon decemmaculatus</i> (Pisces: Poeciliidae)

Autores
Young, Brian Jonathan; López, Gabriela Carina; Cristos, Diego Sebastián; Crespo, Diana Cristina; Somoza, Gustavo Manuel; Carriquiriborde, Pedro
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present study was to assess the responses of the gonopodium morphology and the gonadal and liver histology of adult male Cnesterodon decemmaculatus to sublethal long-term exposure concentrations of 17a-ethinylestradiol (EE2). Two experiments were conducted exposing the fish to waterborne concentrations of EE2 ranging from 20 ng/L to 200 ng/L for 8 wk, 12 wk, and 16 wk. Intersex gonads were observed after 8 wk and 16 wk in fish exposed to 200 ng EE2/L and 100 ng EE2/L, respectively. Oocytes’ development from testis germ cells and replacement of the efferent duct periodic acid-Schiff–positive secretion surrounding spermatozeugmata by parenchymal tissue and duct structure alterations were the major observed changes in the gonads. In contrast, no response was observed in the gonopodium morphology. Liver histology was also altered, showing increasing steatosis, single-cell necrosis to generalized necrosis, and disruption of acinar organization from 100 ng EE2/L to 200 ng EE2/L. In summary, the present results showed that although EE2 was not able to alter the morphology of a developed gonopodium, it was capable of inducing development of testicular oocytes in adult male C. decemmaculatus at environmentally relevant concentrations. Thus, externally normal but intersex C. decemmaculatus males would be expected in the wastewater-receiving streams that the species inhabits. According to the literature, the present study would be the first indicating estrogen-induced intersex in adult male poeciliid.
Centro de Investigaciones del Medioambiente
Materia
Química
Ciencias Exactas
Ecotoxicology
Estrogenic compounds
Testis-ova
Hepatotoxicity
Cnesterodon decemmaculatus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/104815

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oai_identifier_str oai:sedici.unlp.edu.ar:10915/104815
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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Intersex and liver alterations induced by long‐term sublethal exposure to 17α‐ethinylestradiol in adult male <i>Cnesterodon decemmaculatus</i> (Pisces: Poeciliidae)Young, Brian JonathanLópez, Gabriela CarinaCristos, Diego SebastiánCrespo, Diana CristinaSomoza, Gustavo ManuelCarriquiriborde, PedroQuímicaCiencias ExactasEcotoxicologyEstrogenic compoundsTestis-ovaHepatotoxicityCnesterodon decemmaculatusThe aim of the present study was to assess the responses of the gonopodium morphology and the gonadal and liver histology of adult male <i>Cnesterodon decemmaculatus</i> to sublethal long-term exposure concentrations of 17a-ethinylestradiol (EE2). Two experiments were conducted exposing the fish to waterborne concentrations of EE2 ranging from 20 ng/L to 200 ng/L for 8 wk, 12 wk, and 16 wk. Intersex gonads were observed after 8 wk and 16 wk in fish exposed to 200 ng EE2/L and 100 ng EE2/L, respectively. Oocytes’ development from testis germ cells and replacement of the efferent duct periodic acid-Schiff–positive secretion surrounding spermatozeugmata by parenchymal tissue and duct structure alterations were the major observed changes in the gonads. In contrast, no response was observed in the gonopodium morphology. Liver histology was also altered, showing increasing steatosis, single-cell necrosis to generalized necrosis, and disruption of acinar organization from 100 ng EE2/L to 200 ng EE2/L. In summary, the present results showed that although EE2 was not able to alter the morphology of a developed gonopodium, it was capable of inducing development of testicular oocytes in adult male <i>C. decemmaculatus</i> at environmentally relevant concentrations. Thus, externally normal but intersex <i>C. decemmaculatus</i> males would be expected in the wastewater-receiving streams that the species inhabits. According to the literature, the present study would be the first indicating estrogen-induced intersex in adult male poeciliid.Centro de Investigaciones del Medioambiente2016-07-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1738–1745http://sedici.unlp.edu.ar/handle/10915/104815enginfo:eu-repo/semantics/altIdentifier/issn/1552-8618info:eu-repo/semantics/altIdentifier/doi/10.1002/etc.3547info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:14:49Zoai:sedici.unlp.edu.ar:10915/104815Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:14:49.924SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Intersex and liver alterations induced by long‐term sublethal exposure to 17α‐ethinylestradiol in adult male <i>Cnesterodon decemmaculatus</i> (Pisces: Poeciliidae)
title Intersex and liver alterations induced by long‐term sublethal exposure to 17α‐ethinylestradiol in adult male <i>Cnesterodon decemmaculatus</i> (Pisces: Poeciliidae)
spellingShingle Intersex and liver alterations induced by long‐term sublethal exposure to 17α‐ethinylestradiol in adult male <i>Cnesterodon decemmaculatus</i> (Pisces: Poeciliidae)
Young, Brian Jonathan
Química
Ciencias Exactas
Ecotoxicology
Estrogenic compounds
Testis-ova
Hepatotoxicity
Cnesterodon decemmaculatus
title_short Intersex and liver alterations induced by long‐term sublethal exposure to 17α‐ethinylestradiol in adult male <i>Cnesterodon decemmaculatus</i> (Pisces: Poeciliidae)
title_full Intersex and liver alterations induced by long‐term sublethal exposure to 17α‐ethinylestradiol in adult male <i>Cnesterodon decemmaculatus</i> (Pisces: Poeciliidae)
title_fullStr Intersex and liver alterations induced by long‐term sublethal exposure to 17α‐ethinylestradiol in adult male <i>Cnesterodon decemmaculatus</i> (Pisces: Poeciliidae)
title_full_unstemmed Intersex and liver alterations induced by long‐term sublethal exposure to 17α‐ethinylestradiol in adult male <i>Cnesterodon decemmaculatus</i> (Pisces: Poeciliidae)
title_sort Intersex and liver alterations induced by long‐term sublethal exposure to 17α‐ethinylestradiol in adult male <i>Cnesterodon decemmaculatus</i> (Pisces: Poeciliidae)
dc.creator.none.fl_str_mv Young, Brian Jonathan
López, Gabriela Carina
Cristos, Diego Sebastián
Crespo, Diana Cristina
Somoza, Gustavo Manuel
Carriquiriborde, Pedro
author Young, Brian Jonathan
author_facet Young, Brian Jonathan
López, Gabriela Carina
Cristos, Diego Sebastián
Crespo, Diana Cristina
Somoza, Gustavo Manuel
Carriquiriborde, Pedro
author_role author
author2 López, Gabriela Carina
Cristos, Diego Sebastián
Crespo, Diana Cristina
Somoza, Gustavo Manuel
Carriquiriborde, Pedro
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Química
Ciencias Exactas
Ecotoxicology
Estrogenic compounds
Testis-ova
Hepatotoxicity
Cnesterodon decemmaculatus
topic Química
Ciencias Exactas
Ecotoxicology
Estrogenic compounds
Testis-ova
Hepatotoxicity
Cnesterodon decemmaculatus
dc.description.none.fl_txt_mv The aim of the present study was to assess the responses of the gonopodium morphology and the gonadal and liver histology of adult male <i>Cnesterodon decemmaculatus</i> to sublethal long-term exposure concentrations of 17a-ethinylestradiol (EE2). Two experiments were conducted exposing the fish to waterborne concentrations of EE2 ranging from 20 ng/L to 200 ng/L for 8 wk, 12 wk, and 16 wk. Intersex gonads were observed after 8 wk and 16 wk in fish exposed to 200 ng EE2/L and 100 ng EE2/L, respectively. Oocytes’ development from testis germ cells and replacement of the efferent duct periodic acid-Schiff–positive secretion surrounding spermatozeugmata by parenchymal tissue and duct structure alterations were the major observed changes in the gonads. In contrast, no response was observed in the gonopodium morphology. Liver histology was also altered, showing increasing steatosis, single-cell necrosis to generalized necrosis, and disruption of acinar organization from 100 ng EE2/L to 200 ng EE2/L. In summary, the present results showed that although EE2 was not able to alter the morphology of a developed gonopodium, it was capable of inducing development of testicular oocytes in adult male <i>C. decemmaculatus</i> at environmentally relevant concentrations. Thus, externally normal but intersex <i>C. decemmaculatus</i> males would be expected in the wastewater-receiving streams that the species inhabits. According to the literature, the present study would be the first indicating estrogen-induced intersex in adult male poeciliid.
Centro de Investigaciones del Medioambiente
description The aim of the present study was to assess the responses of the gonopodium morphology and the gonadal and liver histology of adult male <i>Cnesterodon decemmaculatus</i> to sublethal long-term exposure concentrations of 17a-ethinylestradiol (EE2). Two experiments were conducted exposing the fish to waterborne concentrations of EE2 ranging from 20 ng/L to 200 ng/L for 8 wk, 12 wk, and 16 wk. Intersex gonads were observed after 8 wk and 16 wk in fish exposed to 200 ng EE2/L and 100 ng EE2/L, respectively. Oocytes’ development from testis germ cells and replacement of the efferent duct periodic acid-Schiff–positive secretion surrounding spermatozeugmata by parenchymal tissue and duct structure alterations were the major observed changes in the gonads. In contrast, no response was observed in the gonopodium morphology. Liver histology was also altered, showing increasing steatosis, single-cell necrosis to generalized necrosis, and disruption of acinar organization from 100 ng EE2/L to 200 ng EE2/L. In summary, the present results showed that although EE2 was not able to alter the morphology of a developed gonopodium, it was capable of inducing development of testicular oocytes in adult male <i>C. decemmaculatus</i> at environmentally relevant concentrations. Thus, externally normal but intersex <i>C. decemmaculatus</i> males would be expected in the wastewater-receiving streams that the species inhabits. According to the literature, the present study would be the first indicating estrogen-induced intersex in adult male poeciliid.
publishDate 2016
dc.date.none.fl_str_mv 2016-07-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/104815
url http://sedici.unlp.edu.ar/handle/10915/104815
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1552-8618
info:eu-repo/semantics/altIdentifier/doi/10.1002/etc.3547
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
1738–1745
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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