Fatty acid-binding proteins in Echinococcus spp.: the family has grown

Autores
Pórfido, Jorge Luis; Herz, Michaela; Kiss, Ferenc; Kamenetzky, Laura; Brehm, Klaus; Rosenzvit, Mara Cecilia; Córsico, Betina; Franchini, Gisela Raquel
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fatty acid-binding proteins (FABPs) are small intracellular proteins that reversibly bind fatty acids and other hydrophobic ligands. In cestodes, due to their inability to synthesise fatty acids de novo, FABPs have been proposed as essential proteins, and thus, as possible drug targets and/or carriers against these parasites. We performed data mining in Echinococcus multilocularis and Echinococcus granulosus genomes in order to test whether this family of proteins is more complex than previously reported. By exploring the genomes of E. multilocularis and E. granulosus, six genes coding for FABPs were found in each organism. In the case of E. granulosus, all of them have different coding sequences, whereas in E. multilocularis, two of the genes code for the same protein. Remarkably, one of the genes (in both cestodes) encodes a FABP with a C-terminal extension unusual for this family of proteins. The newly described genes present variations in their structure in comparison with previously described FABP genes in Echinococcus spp. The coding sequences for E. multilocularis were validated by cloning and sequencing. Moreover, differential expression patterns of FABPs were observed at different stages of the life cycle of E. multilocularis by exploring transcriptomic data from several sources. In summary, FABP family in cestodes is far more complex than previously thought and includes new members that seem to be only present in flatworms.
Instituto de Investigaciones Bioquímicas de La Plata
Materia
Bioquímica
Ciencias Exactas
Echinococcus multilocularis
FABPs
Fatty acids
Cestodes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/140067

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spelling Fatty acid-binding proteins in Echinococcus spp.: the family has grownPórfido, Jorge LuisHerz, MichaelaKiss, FerencKamenetzky, LauraBrehm, KlausRosenzvit, Mara CeciliaCórsico, BetinaFranchini, Gisela RaquelBioquímicaCiencias ExactasEchinococcus multilocularisFABPsFatty acidsCestodesFatty acid-binding proteins (FABPs) are small intracellular proteins that reversibly bind fatty acids and other hydrophobic ligands. In cestodes, due to their inability to synthesise fatty acids de novo, FABPs have been proposed as essential proteins, and thus, as possible drug targets and/or carriers against these parasites. We performed data mining in Echinococcus multilocularis and Echinococcus granulosus genomes in order to test whether this family of proteins is more complex than previously reported. By exploring the genomes of E. multilocularis and E. granulosus, six genes coding for FABPs were found in each organism. In the case of E. granulosus, all of them have different coding sequences, whereas in E. multilocularis, two of the genes code for the same protein. Remarkably, one of the genes (in both cestodes) encodes a FABP with a C-terminal extension unusual for this family of proteins. The newly described genes present variations in their structure in comparison with previously described FABP genes in Echinococcus spp. The coding sequences for E. multilocularis were validated by cloning and sequencing. Moreover, differential expression patterns of FABPs were observed at different stages of the life cycle of E. multilocularis by exploring transcriptomic data from several sources. In summary, FABP family in cestodes is far more complex than previously thought and includes new members that seem to be only present in flatworms.Instituto de Investigaciones Bioquímicas de La Plata2020-03-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1401-1408http://sedici.unlp.edu.ar/handle/10915/140067enginfo:eu-repo/semantics/altIdentifier/issn/1432-1955info:eu-repo/semantics/altIdentifier/issn/0932-0113info:eu-repo/semantics/altIdentifier/doi/10.1007/s00436-020-06631-5info:eu-repo/semantics/altIdentifier/pmid/32130486info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:07Zoai:sedici.unlp.edu.ar:10915/140067Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:07.404SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Fatty acid-binding proteins in Echinococcus spp.: the family has grown
title Fatty acid-binding proteins in Echinococcus spp.: the family has grown
spellingShingle Fatty acid-binding proteins in Echinococcus spp.: the family has grown
Pórfido, Jorge Luis
Bioquímica
Ciencias Exactas
Echinococcus multilocularis
FABPs
Fatty acids
Cestodes
title_short Fatty acid-binding proteins in Echinococcus spp.: the family has grown
title_full Fatty acid-binding proteins in Echinococcus spp.: the family has grown
title_fullStr Fatty acid-binding proteins in Echinococcus spp.: the family has grown
title_full_unstemmed Fatty acid-binding proteins in Echinococcus spp.: the family has grown
title_sort Fatty acid-binding proteins in Echinococcus spp.: the family has grown
dc.creator.none.fl_str_mv Pórfido, Jorge Luis
Herz, Michaela
Kiss, Ferenc
Kamenetzky, Laura
Brehm, Klaus
Rosenzvit, Mara Cecilia
Córsico, Betina
Franchini, Gisela Raquel
author Pórfido, Jorge Luis
author_facet Pórfido, Jorge Luis
Herz, Michaela
Kiss, Ferenc
Kamenetzky, Laura
Brehm, Klaus
Rosenzvit, Mara Cecilia
Córsico, Betina
Franchini, Gisela Raquel
author_role author
author2 Herz, Michaela
Kiss, Ferenc
Kamenetzky, Laura
Brehm, Klaus
Rosenzvit, Mara Cecilia
Córsico, Betina
Franchini, Gisela Raquel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bioquímica
Ciencias Exactas
Echinococcus multilocularis
FABPs
Fatty acids
Cestodes
topic Bioquímica
Ciencias Exactas
Echinococcus multilocularis
FABPs
Fatty acids
Cestodes
dc.description.none.fl_txt_mv Fatty acid-binding proteins (FABPs) are small intracellular proteins that reversibly bind fatty acids and other hydrophobic ligands. In cestodes, due to their inability to synthesise fatty acids de novo, FABPs have been proposed as essential proteins, and thus, as possible drug targets and/or carriers against these parasites. We performed data mining in Echinococcus multilocularis and Echinococcus granulosus genomes in order to test whether this family of proteins is more complex than previously reported. By exploring the genomes of E. multilocularis and E. granulosus, six genes coding for FABPs were found in each organism. In the case of E. granulosus, all of them have different coding sequences, whereas in E. multilocularis, two of the genes code for the same protein. Remarkably, one of the genes (in both cestodes) encodes a FABP with a C-terminal extension unusual for this family of proteins. The newly described genes present variations in their structure in comparison with previously described FABP genes in Echinococcus spp. The coding sequences for E. multilocularis were validated by cloning and sequencing. Moreover, differential expression patterns of FABPs were observed at different stages of the life cycle of E. multilocularis by exploring transcriptomic data from several sources. In summary, FABP family in cestodes is far more complex than previously thought and includes new members that seem to be only present in flatworms.
Instituto de Investigaciones Bioquímicas de La Plata
description Fatty acid-binding proteins (FABPs) are small intracellular proteins that reversibly bind fatty acids and other hydrophobic ligands. In cestodes, due to their inability to synthesise fatty acids de novo, FABPs have been proposed as essential proteins, and thus, as possible drug targets and/or carriers against these parasites. We performed data mining in Echinococcus multilocularis and Echinococcus granulosus genomes in order to test whether this family of proteins is more complex than previously reported. By exploring the genomes of E. multilocularis and E. granulosus, six genes coding for FABPs were found in each organism. In the case of E. granulosus, all of them have different coding sequences, whereas in E. multilocularis, two of the genes code for the same protein. Remarkably, one of the genes (in both cestodes) encodes a FABP with a C-terminal extension unusual for this family of proteins. The newly described genes present variations in their structure in comparison with previously described FABP genes in Echinococcus spp. The coding sequences for E. multilocularis were validated by cloning and sequencing. Moreover, differential expression patterns of FABPs were observed at different stages of the life cycle of E. multilocularis by exploring transcriptomic data from several sources. In summary, FABP family in cestodes is far more complex than previously thought and includes new members that seem to be only present in flatworms.
publishDate 2020
dc.date.none.fl_str_mv 2020-03-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/140067
url http://sedici.unlp.edu.ar/handle/10915/140067
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1432-1955
info:eu-repo/semantics/altIdentifier/issn/0932-0113
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00436-020-06631-5
info:eu-repo/semantics/altIdentifier/pmid/32130486
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
1401-1408
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instname:Universidad Nacional de La Plata
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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