R-spondin3 Is Associated with Basal-Progenitor Behavior in Normal and Tumor Mammary Cells

Autores
Tocci, Johanna M.; Felcher, Carla M.; García Solá, Martín E.; Goddio, María Victoria; Zimberlin, María Noel; Rubinstein, Natalia; Srebrow, Anabella; Coso, Omar A.; Abba, Martín Carlos; Meiss, Roberto P.; Kordon, Edith C.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
R-spondin3 (RSPO3) is a member of a family of secreted proteins that enhance Wnt signaling pathways in diverse processes, including cancer. However, the role of RSPO3 in mammary gland and breast cancer development remains unclear. In this study, we show that RSPO3 is expressed in the basal stem cell-enriched compartment of normal mouse mammary glands but is absent from committed mature luminal cells in which exogenous RSPO3 impairs lactogenic differentiation. RSPO3 knockdown in basal-like mouse mammary tumor cells reduced canonical Wnt signaling, epithelial-to-mesenchymal transition-like features, migration capacity, and tumor formation in vivo. Conversely, RSPO3 overexpression, which was associated with some LGR and RUNX factors, highly correlated with the basal-like subtype among patients with breast cancer. Thus, we identified RSPO3 as a novel key modulator of breast cancer development and a potential target for treatment of basal-like breast cancers.
Facultad de Ciencias Médicas
Centro de Investigaciones Inmunológicas Básicas y Aplicadas
Materia
Medicina
R-spondin3 (RSPO3)
Cancer
Breast cancer
Mammary cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/124844
Acceder
id SEDICI_2e4925635216211cb8fd57106c466491
oai_identifier_str oai:sedici.unlp.edu.ar:10915/124844
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling R-spondin3 Is Associated with Basal-Progenitor Behavior in Normal and Tumor Mammary CellsTocci, Johanna M.Felcher, Carla M.García Solá, Martín E.Goddio, María VictoriaZimberlin, María NoelRubinstein, NataliaSrebrow, AnabellaCoso, Omar A.Abba, Martín CarlosMeiss, Roberto P.Kordon, Edith C.MedicinaR-spondin3 (RSPO3)CancerBreast cancerMammary cellsR-spondin3 (RSPO3) is a member of a family of secreted proteins that enhance Wnt signaling pathways in diverse processes, including cancer. However, the role of RSPO3 in mammary gland and breast cancer development remains unclear. In this study, we show that RSPO3 is expressed in the basal stem cell-enriched compartment of normal mouse mammary glands but is absent from committed mature luminal cells in which exogenous RSPO3 impairs lactogenic differentiation. RSPO3 knockdown in basal-like mouse mammary tumor cells reduced canonical Wnt signaling, epithelial-to-mesenchymal transition-like features, migration capacity, and tumor formation <i>in vivo</i>. Conversely, RSPO3 overexpression, which was associated with some LGR and RUNX factors, highly correlated with the basal-like subtype among patients with breast cancer. Thus, we identified RSPO3 as a novel key modulator of breast cancer development and a potential target for treatment of basal-like breast cancers.Facultad de Ciencias MédicasCentro de Investigaciones Inmunológicas Básicas y Aplicadas2018-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf4497-4511http://sedici.unlp.edu.ar/handle/10915/124844enginfo:eu-repo/semantics/altIdentifier/issn/1538-7445info:eu-repo/semantics/altIdentifier/issn/0008-5472info:eu-repo/semantics/altIdentifier/pmid/29748375info:eu-repo/semantics/altIdentifier/doi/10.1158/0008-5472.can-17-2676info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:01:53Zoai:sedici.unlp.edu.ar:10915/124844Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:01:53.779SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv R-spondin3 Is Associated with Basal-Progenitor Behavior in Normal and Tumor Mammary Cells
title R-spondin3 Is Associated with Basal-Progenitor Behavior in Normal and Tumor Mammary Cells
spellingShingle R-spondin3 Is Associated with Basal-Progenitor Behavior in Normal and Tumor Mammary Cells
Tocci, Johanna M.
Medicina
R-spondin3 (RSPO3)
Cancer
Breast cancer
Mammary cells
title_short R-spondin3 Is Associated with Basal-Progenitor Behavior in Normal and Tumor Mammary Cells
title_full R-spondin3 Is Associated with Basal-Progenitor Behavior in Normal and Tumor Mammary Cells
title_fullStr R-spondin3 Is Associated with Basal-Progenitor Behavior in Normal and Tumor Mammary Cells
title_full_unstemmed R-spondin3 Is Associated with Basal-Progenitor Behavior in Normal and Tumor Mammary Cells
title_sort R-spondin3 Is Associated with Basal-Progenitor Behavior in Normal and Tumor Mammary Cells
dc.creator.none.fl_str_mv Tocci, Johanna M.
Felcher, Carla M.
García Solá, Martín E.
Goddio, María Victoria
Zimberlin, María Noel
Rubinstein, Natalia
Srebrow, Anabella
Coso, Omar A.
Abba, Martín Carlos
Meiss, Roberto P.
Kordon, Edith C.
author Tocci, Johanna M.
author_facet Tocci, Johanna M.
Felcher, Carla M.
García Solá, Martín E.
Goddio, María Victoria
Zimberlin, María Noel
Rubinstein, Natalia
Srebrow, Anabella
Coso, Omar A.
Abba, Martín Carlos
Meiss, Roberto P.
Kordon, Edith C.
author_role author
author2 Felcher, Carla M.
García Solá, Martín E.
Goddio, María Victoria
Zimberlin, María Noel
Rubinstein, Natalia
Srebrow, Anabella
Coso, Omar A.
Abba, Martín Carlos
Meiss, Roberto P.
Kordon, Edith C.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Medicina
R-spondin3 (RSPO3)
Cancer
Breast cancer
Mammary cells
topic Medicina
R-spondin3 (RSPO3)
Cancer
Breast cancer
Mammary cells
dc.description.none.fl_txt_mv R-spondin3 (RSPO3) is a member of a family of secreted proteins that enhance Wnt signaling pathways in diverse processes, including cancer. However, the role of RSPO3 in mammary gland and breast cancer development remains unclear. In this study, we show that RSPO3 is expressed in the basal stem cell-enriched compartment of normal mouse mammary glands but is absent from committed mature luminal cells in which exogenous RSPO3 impairs lactogenic differentiation. RSPO3 knockdown in basal-like mouse mammary tumor cells reduced canonical Wnt signaling, epithelial-to-mesenchymal transition-like features, migration capacity, and tumor formation <i>in vivo</i>. Conversely, RSPO3 overexpression, which was associated with some LGR and RUNX factors, highly correlated with the basal-like subtype among patients with breast cancer. Thus, we identified RSPO3 as a novel key modulator of breast cancer development and a potential target for treatment of basal-like breast cancers.
Facultad de Ciencias Médicas
Centro de Investigaciones Inmunológicas Básicas y Aplicadas
description R-spondin3 (RSPO3) is a member of a family of secreted proteins that enhance Wnt signaling pathways in diverse processes, including cancer. However, the role of RSPO3 in mammary gland and breast cancer development remains unclear. In this study, we show that RSPO3 is expressed in the basal stem cell-enriched compartment of normal mouse mammary glands but is absent from committed mature luminal cells in which exogenous RSPO3 impairs lactogenic differentiation. RSPO3 knockdown in basal-like mouse mammary tumor cells reduced canonical Wnt signaling, epithelial-to-mesenchymal transition-like features, migration capacity, and tumor formation <i>in vivo</i>. Conversely, RSPO3 overexpression, which was associated with some LGR and RUNX factors, highly correlated with the basal-like subtype among patients with breast cancer. Thus, we identified RSPO3 as a novel key modulator of breast cancer development and a potential target for treatment of basal-like breast cancers.
publishDate 2018
dc.date.none.fl_str_mv 2018-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/124844
url http://sedici.unlp.edu.ar/handle/10915/124844
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1538-7445
info:eu-repo/semantics/altIdentifier/issn/0008-5472
info:eu-repo/semantics/altIdentifier/pmid/29748375
info:eu-repo/semantics/altIdentifier/doi/10.1158/0008-5472.can-17-2676
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
4497-4511
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1842260516721917952
score 13.13397

Publicaciones similares