Technique for the identification of osmophores in flowers of herbarium material (TIOFH)

Autores
Hernández, Marcelo Paulo; Katinas, Liliana
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The histochemical studies that search for osmophores differ in the protocol they follow for fixation and discoloration of the samples, and also in the type and number of stains utilized. Despite these differences, all the studies have one point in common: the use of fresh material either collected directly in the field or cultivated in botanical gardens and greenhouses. This is an obvious limitation for the studies of osmophores. Flower parts of herbarium specimens of different dates of collection and different plant families were exposed to variable times and percentages of discoloration agents and under different stains for finding if it is possible to positively test osmophores in this type of material. We obtained positive results discoloring the samples with ethyl alcohol 96° and sodium hypochlorite, and staining with Lugol, Oil Red O, and Neutral Red (TIOFH). A protocol (TIOFH3) for osmophore testing that combines these three stains into a single procedure is proposed.
Laboratorio de Morfología Comparada de Espermatófitas
Materia
Ciencias Agrarias
Biología
Floral osmophores
Protocol
Herbarium material
Histochemical technique
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/131989
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/131989
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Technique for the identification of osmophores in flowers of herbarium material (TIOFH)Hernández, Marcelo PauloKatinas, LilianaCiencias AgrariasBiologíaFloral osmophoresProtocolHerbarium materialHistochemical techniqueThe histochemical studies that search for osmophores differ in the protocol they follow for fixation and discoloration of the samples, and also in the type and number of stains utilized. Despite these differences, all the studies have one point in common: the use of fresh material either collected directly in the field or cultivated in botanical gardens and greenhouses. This is an obvious limitation for the studies of osmophores. Flower parts of herbarium specimens of different dates of collection and different plant families were exposed to variable times and percentages of discoloration agents and under different stains for finding if it is possible to positively test osmophores in this type of material. We obtained positive results discoloring the samples with ethyl alcohol 96° and sodium hypochlorite, and staining with Lugol, Oil Red O, and Neutral Red (TIOFH). A protocol (TIOFH3) for osmophore testing that combines these three stains into a single procedure is proposed.Laboratorio de Morfología Comparada de Espermatófitas2019-06-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1753-1765http://sedici.unlp.edu.ar/handle/10915/131989enginfo:eu-repo/semantics/altIdentifier/issn/1615-6102info:eu-repo/semantics/altIdentifier/issn/0033-183xinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00709-019-01398-8info:eu-repo/semantics/altIdentifier/pmid/31183550info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:23:33Zoai:sedici.unlp.edu.ar:10915/131989Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:23:34.234SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Technique for the identification of osmophores in flowers of herbarium material (TIOFH)
title Technique for the identification of osmophores in flowers of herbarium material (TIOFH)
spellingShingle Technique for the identification of osmophores in flowers of herbarium material (TIOFH)
Hernández, Marcelo Paulo
Ciencias Agrarias
Biología
Floral osmophores
Protocol
Herbarium material
Histochemical technique
title_short Technique for the identification of osmophores in flowers of herbarium material (TIOFH)
title_full Technique for the identification of osmophores in flowers of herbarium material (TIOFH)
title_fullStr Technique for the identification of osmophores in flowers of herbarium material (TIOFH)
title_full_unstemmed Technique for the identification of osmophores in flowers of herbarium material (TIOFH)
title_sort Technique for the identification of osmophores in flowers of herbarium material (TIOFH)
dc.creator.none.fl_str_mv Hernández, Marcelo Paulo
Katinas, Liliana
author Hernández, Marcelo Paulo
author_facet Hernández, Marcelo Paulo
Katinas, Liliana
author_role author
author2 Katinas, Liliana
author2_role author
dc.subject.none.fl_str_mv Ciencias Agrarias
Biología
Floral osmophores
Protocol
Herbarium material
Histochemical technique
topic Ciencias Agrarias
Biología
Floral osmophores
Protocol
Herbarium material
Histochemical technique
dc.description.none.fl_txt_mv The histochemical studies that search for osmophores differ in the protocol they follow for fixation and discoloration of the samples, and also in the type and number of stains utilized. Despite these differences, all the studies have one point in common: the use of fresh material either collected directly in the field or cultivated in botanical gardens and greenhouses. This is an obvious limitation for the studies of osmophores. Flower parts of herbarium specimens of different dates of collection and different plant families were exposed to variable times and percentages of discoloration agents and under different stains for finding if it is possible to positively test osmophores in this type of material. We obtained positive results discoloring the samples with ethyl alcohol 96° and sodium hypochlorite, and staining with Lugol, Oil Red O, and Neutral Red (TIOFH). A protocol (TIOFH3) for osmophore testing that combines these three stains into a single procedure is proposed.
Laboratorio de Morfología Comparada de Espermatófitas
description The histochemical studies that search for osmophores differ in the protocol they follow for fixation and discoloration of the samples, and also in the type and number of stains utilized. Despite these differences, all the studies have one point in common: the use of fresh material either collected directly in the field or cultivated in botanical gardens and greenhouses. This is an obvious limitation for the studies of osmophores. Flower parts of herbarium specimens of different dates of collection and different plant families were exposed to variable times and percentages of discoloration agents and under different stains for finding if it is possible to positively test osmophores in this type of material. We obtained positive results discoloring the samples with ethyl alcohol 96° and sodium hypochlorite, and staining with Lugol, Oil Red O, and Neutral Red (TIOFH). A protocol (TIOFH3) for osmophore testing that combines these three stains into a single procedure is proposed.
publishDate 2019
dc.date.none.fl_str_mv 2019-06-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/131989
url http://sedici.unlp.edu.ar/handle/10915/131989
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1615-6102
info:eu-repo/semantics/altIdentifier/issn/0033-183x
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00709-019-01398-8
info:eu-repo/semantics/altIdentifier/pmid/31183550
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
1753-1765
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.22299

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