Immunochemical reactivity of soybean β-conglycinin subunits
- Autores
- Petruccelli, Silvana; Chirdo, Fernando Gabriel; Añón, María Cristina
- Año de publicación
- 2005
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The immunochemical reactivity of two polyclonal sera raised against soybean purified αα' and β subunits was analysed. We show that anti-αα' serum mainly reacts with αα' subunits and β' subunit, but it does not recognize β subunit. In addition, β subunit is not able to compete with αα' subunit for anti-αα' serum recognition sites; therefore the main immunogenic region of αα' subunits is the extension region. Both anti-αα' and anti-β sera were able to recognize E. coli produced α subunit, consequently glycosylation is apparently not required for antigen-antibody interaction. Anti-β subunit serum shows strong reactivity against soybean produced β-subunit and recombinant α subunit, while it shows weak recognition of soybean purified αα' subunits. A mutant of α subunit with the N terminal β barrel region and C terminal α helices deleted, failed to be recognized by anti-β serum. For that reason although N and C terminal domains are structurally equivalent, their immunochemical reactivity is different.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos - Materia
-
Química
β-conglycinin
Recombinant soybean subunits
Immunoreactivity
Soybean 7S globulin - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/123858
Ver los metadatos del registro completo
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Immunochemical reactivity of soybean β-conglycinin subunitsPetruccelli, SilvanaChirdo, Fernando GabrielAñón, María CristinaQuímicaβ-conglycininRecombinant soybean subunitsImmunoreactivitySoybean 7S globulinThe immunochemical reactivity of two polyclonal sera raised against soybean purified αα' and β subunits was analysed. We show that anti-αα' serum mainly reacts with αα' subunits and β' subunit, but it does not recognize β subunit. In addition, β subunit is not able to compete with αα' subunit for anti-αα' serum recognition sites; therefore the main immunogenic region of αα' subunits is the extension region. Both anti-αα' and anti-β sera were able to recognize E. coli produced α subunit, consequently glycosylation is apparently not required for antigen-antibody interaction. Anti-β subunit serum shows strong reactivity against soybean produced β-subunit and recombinant α subunit, while it shows weak recognition of soybean purified αα' subunits. A mutant of α subunit with the N terminal β barrel region and C terminal α helices deleted, failed to be recognized by anti-β serum. For that reason although N and C terminal domains are structurally equivalent, their immunochemical reactivity is different.Centro de Investigación y Desarrollo en Criotecnología de Alimentos2005info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf17-28http://sedici.unlp.edu.ar/handle/10915/123858enginfo:eu-repo/semantics/altIdentifier/issn/0954-0105info:eu-repo/semantics/altIdentifier/issn/1465-3443info:eu-repo/semantics/altIdentifier/doi/10.1080/09540100500054727info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:29:28Zoai:sedici.unlp.edu.ar:10915/123858Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:29:28.882SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Immunochemical reactivity of soybean β-conglycinin subunits |
title |
Immunochemical reactivity of soybean β-conglycinin subunits |
spellingShingle |
Immunochemical reactivity of soybean β-conglycinin subunits Petruccelli, Silvana Química β-conglycinin Recombinant soybean subunits Immunoreactivity Soybean 7S globulin |
title_short |
Immunochemical reactivity of soybean β-conglycinin subunits |
title_full |
Immunochemical reactivity of soybean β-conglycinin subunits |
title_fullStr |
Immunochemical reactivity of soybean β-conglycinin subunits |
title_full_unstemmed |
Immunochemical reactivity of soybean β-conglycinin subunits |
title_sort |
Immunochemical reactivity of soybean β-conglycinin subunits |
dc.creator.none.fl_str_mv |
Petruccelli, Silvana Chirdo, Fernando Gabriel Añón, María Cristina |
author |
Petruccelli, Silvana |
author_facet |
Petruccelli, Silvana Chirdo, Fernando Gabriel Añón, María Cristina |
author_role |
author |
author2 |
Chirdo, Fernando Gabriel Añón, María Cristina |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Química β-conglycinin Recombinant soybean subunits Immunoreactivity Soybean 7S globulin |
topic |
Química β-conglycinin Recombinant soybean subunits Immunoreactivity Soybean 7S globulin |
dc.description.none.fl_txt_mv |
The immunochemical reactivity of two polyclonal sera raised against soybean purified αα' and β subunits was analysed. We show that anti-αα' serum mainly reacts with αα' subunits and β' subunit, but it does not recognize β subunit. In addition, β subunit is not able to compete with αα' subunit for anti-αα' serum recognition sites; therefore the main immunogenic region of αα' subunits is the extension region. Both anti-αα' and anti-β sera were able to recognize E. coli produced α subunit, consequently glycosylation is apparently not required for antigen-antibody interaction. Anti-β subunit serum shows strong reactivity against soybean produced β-subunit and recombinant α subunit, while it shows weak recognition of soybean purified αα' subunits. A mutant of α subunit with the N terminal β barrel region and C terminal α helices deleted, failed to be recognized by anti-β serum. For that reason although N and C terminal domains are structurally equivalent, their immunochemical reactivity is different. Centro de Investigación y Desarrollo en Criotecnología de Alimentos |
description |
The immunochemical reactivity of two polyclonal sera raised against soybean purified αα' and β subunits was analysed. We show that anti-αα' serum mainly reacts with αα' subunits and β' subunit, but it does not recognize β subunit. In addition, β subunit is not able to compete with αα' subunit for anti-αα' serum recognition sites; therefore the main immunogenic region of αα' subunits is the extension region. Both anti-αα' and anti-β sera were able to recognize E. coli produced α subunit, consequently glycosylation is apparently not required for antigen-antibody interaction. Anti-β subunit serum shows strong reactivity against soybean produced β-subunit and recombinant α subunit, while it shows weak recognition of soybean purified αα' subunits. A mutant of α subunit with the N terminal β barrel region and C terminal α helices deleted, failed to be recognized by anti-β serum. For that reason although N and C terminal domains are structurally equivalent, their immunochemical reactivity is different. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/123858 |
url |
http://sedici.unlp.edu.ar/handle/10915/123858 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/0954-0105 info:eu-repo/semantics/altIdentifier/issn/1465-3443 info:eu-repo/semantics/altIdentifier/doi/10.1080/09540100500054727 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf 17-28 |
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reponame:SEDICI (UNLP) instname:Universidad Nacional de La Plata instacron:UNLP |
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SEDICI (UNLP) - Universidad Nacional de La Plata |
repository.mail.fl_str_mv |
alira@sedici.unlp.edu.ar |
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