Immunochemical reactivity of soybean β-conglycinin subunits

Autores
Petruccelli, Silvana; Chirdo, Fernando Gabriel; Añón, María Cristina
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The immunochemical reactivity of two polyclonal sera raised against soybean purified αα' and β subunits was analysed. We show that anti-αα' serum mainly reacts with αα' subunits and β' subunit, but it does not recognize β subunit. In addition, β subunit is not able to compete with αα' subunit for anti-αα' serum recognition sites; therefore the main immunogenic region of αα' subunits is the extension region. Both anti-αα' and anti-β sera were able to recognize E. coli produced α subunit, consequently glycosylation is apparently not required for antigen-antibody interaction. Anti-β subunit serum shows strong reactivity against soybean produced β-subunit and recombinant α subunit, while it shows weak recognition of soybean purified αα' subunits. A mutant of α subunit with the N terminal β barrel region and C terminal α helices deleted, failed to be recognized by anti-β serum. For that reason although N and C terminal domains are structurally equivalent, their immunochemical reactivity is different.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Materia
Química
β-conglycinin
Recombinant soybean subunits
Immunoreactivity
Soybean 7S globulin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/123858
Acceder
id SEDICI_26764820f8ac2aa4afd72e00148dd38d
oai_identifier_str oai:sedici.unlp.edu.ar:10915/123858
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Immunochemical reactivity of soybean β-conglycinin subunitsPetruccelli, SilvanaChirdo, Fernando GabrielAñón, María CristinaQuímicaβ-conglycininRecombinant soybean subunitsImmunoreactivitySoybean 7S globulinThe immunochemical reactivity of two polyclonal sera raised against soybean purified αα' and β subunits was analysed. We show that anti-αα' serum mainly reacts with αα' subunits and β' subunit, but it does not recognize β subunit. In addition, β subunit is not able to compete with αα' subunit for anti-αα' serum recognition sites; therefore the main immunogenic region of αα' subunits is the extension region. Both anti-αα' and anti-β sera were able to recognize E. coli produced α subunit, consequently glycosylation is apparently not required for antigen-antibody interaction. Anti-β subunit serum shows strong reactivity against soybean produced β-subunit and recombinant α subunit, while it shows weak recognition of soybean purified αα' subunits. A mutant of α subunit with the N terminal β barrel region and C terminal α helices deleted, failed to be recognized by anti-β serum. For that reason although N and C terminal domains are structurally equivalent, their immunochemical reactivity is different.Centro de Investigación y Desarrollo en Criotecnología de Alimentos2005info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf17-28http://sedici.unlp.edu.ar/handle/10915/123858enginfo:eu-repo/semantics/altIdentifier/issn/0954-0105info:eu-repo/semantics/altIdentifier/issn/1465-3443info:eu-repo/semantics/altIdentifier/doi/10.1080/09540100500054727info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:10:24Zoai:sedici.unlp.edu.ar:10915/123858Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:10:24.444SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Immunochemical reactivity of soybean β-conglycinin subunits
title Immunochemical reactivity of soybean β-conglycinin subunits
spellingShingle Immunochemical reactivity of soybean β-conglycinin subunits
Petruccelli, Silvana
Química
β-conglycinin
Recombinant soybean subunits
Immunoreactivity
Soybean 7S globulin
title_short Immunochemical reactivity of soybean β-conglycinin subunits
title_full Immunochemical reactivity of soybean β-conglycinin subunits
title_fullStr Immunochemical reactivity of soybean β-conglycinin subunits
title_full_unstemmed Immunochemical reactivity of soybean β-conglycinin subunits
title_sort Immunochemical reactivity of soybean β-conglycinin subunits
dc.creator.none.fl_str_mv Petruccelli, Silvana
Chirdo, Fernando Gabriel
Añón, María Cristina
author Petruccelli, Silvana
author_facet Petruccelli, Silvana
Chirdo, Fernando Gabriel
Añón, María Cristina
author_role author
author2 Chirdo, Fernando Gabriel
Añón, María Cristina
author2_role author
author
dc.subject.none.fl_str_mv Química
β-conglycinin
Recombinant soybean subunits
Immunoreactivity
Soybean 7S globulin
topic Química
β-conglycinin
Recombinant soybean subunits
Immunoreactivity
Soybean 7S globulin
dc.description.none.fl_txt_mv The immunochemical reactivity of two polyclonal sera raised against soybean purified αα' and β subunits was analysed. We show that anti-αα' serum mainly reacts with αα' subunits and β' subunit, but it does not recognize β subunit. In addition, β subunit is not able to compete with αα' subunit for anti-αα' serum recognition sites; therefore the main immunogenic region of αα' subunits is the extension region. Both anti-αα' and anti-β sera were able to recognize E. coli produced α subunit, consequently glycosylation is apparently not required for antigen-antibody interaction. Anti-β subunit serum shows strong reactivity against soybean produced β-subunit and recombinant α subunit, while it shows weak recognition of soybean purified αα' subunits. A mutant of α subunit with the N terminal β barrel region and C terminal α helices deleted, failed to be recognized by anti-β serum. For that reason although N and C terminal domains are structurally equivalent, their immunochemical reactivity is different.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
description The immunochemical reactivity of two polyclonal sera raised against soybean purified αα' and β subunits was analysed. We show that anti-αα' serum mainly reacts with αα' subunits and β' subunit, but it does not recognize β subunit. In addition, β subunit is not able to compete with αα' subunit for anti-αα' serum recognition sites; therefore the main immunogenic region of αα' subunits is the extension region. Both anti-αα' and anti-β sera were able to recognize E. coli produced α subunit, consequently glycosylation is apparently not required for antigen-antibody interaction. Anti-β subunit serum shows strong reactivity against soybean produced β-subunit and recombinant α subunit, while it shows weak recognition of soybean purified αα' subunits. A mutant of α subunit with the N terminal β barrel region and C terminal α helices deleted, failed to be recognized by anti-β serum. For that reason although N and C terminal domains are structurally equivalent, their immunochemical reactivity is different.
publishDate 2005
dc.date.none.fl_str_mv 2005
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/123858
url http://sedici.unlp.edu.ar/handle/10915/123858
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0954-0105
info:eu-repo/semantics/altIdentifier/issn/1465-3443
info:eu-repo/semantics/altIdentifier/doi/10.1080/09540100500054727
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
17-28
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 12.982451

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