Dynamics of bacteriological and cytological changes in the uterus of postpartum dairy cows

Autores
Prunner, I.; Pothmann, H.; Wagener, K.; Giuliodori, Mauricio Javier; Huber, J.; Ehling Schulz, M.; Drillich, M.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objectives of this study were to characterize clinical, intrauterine, bacteriologic and cytologic changes during the first month after parturition in healthy dairy cows and in cows with subclinical endometritis (SE) or clinical endometritis (CE). Furthermore, risk factors related to clinical bacteriologic and cytologic findings were determined. A total of 170 calvings were enrolled, and intrauterine samples were collected on Days 0, 3, 9, 15, 21, and 28 postpartum using the cytobrush technique. The presence of Escherichia coli and Trueperella pyogenes was determined by Fourier transform infrared spectroscopy. The cows were categorized according to their uterine health status (UHS) on Day 21 as healthy (clear or absent vaginal discharge and <5% polymorphonuclear cells [PMN] in the cytologic sample), SE (clear or absent vaginal discharge and 5% PMN), or CE (vaginal mucus containingany signs of pus). The prevalence of SE and CE on Day 21 was 27.9% and 58.4%, respectively. Generally, samples from cows with SE and CE showed a greater bacterial growth density (BGD) than those from healthy cows. The BGD tended to be affected by the interaction of time by UHS (P ¼ 0.057). Differences between healthy, SE, and CE cows were found from Day 3 to the last sampling day. Furthermore, the percentage of PMN differed between healthy, SE, and CE cows and was affected by time in a cubic way (decrease/increase/decrease). Overall, E coli was found in 25.4% of the samples, and T pyogenes was identified in 30.2% of the samples. The risk for CE was increased by BGD and the presence of T pyogenes. Conversely, the presence of E coli had no effect on the risk of CE or the risk of SE. The risk for an infection with T pyogenes was greater in the first-parity cows and in cows with assisted calving. In conclusion, changes in BGD and proportion of PMN varied with the UHS (healthy, SE, and CE), which was affected by the presence of T pyogenes but not E coli.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
Uterine disease
Bacteria
Cytology
Dairy cow
Fourier transform infrared spectroscopy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/159586

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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Dynamics of bacteriological and cytological changes in the uterus of postpartum dairy cowsPrunner, I.Pothmann, H.Wagener, K.Giuliodori, Mauricio JavierHuber, J.Ehling Schulz, M.Drillich, M.Ciencias VeterinariasUterine diseaseBacteriaCytologyDairy cowFourier transform infrared spectroscopyThe objectives of this study were to characterize clinical, intrauterine, bacteriologic and cytologic changes during the first month after parturition in healthy dairy cows and in cows with subclinical endometritis (SE) or clinical endometritis (CE). Furthermore, risk factors related to clinical bacteriologic and cytologic findings were determined. A total of 170 calvings were enrolled, and intrauterine samples were collected on Days 0, 3, 9, 15, 21, and 28 postpartum using the cytobrush technique. The presence of Escherichia coli and Trueperella pyogenes was determined by Fourier transform infrared spectroscopy. The cows were categorized according to their uterine health status (UHS) on Day 21 as healthy (clear or absent vaginal discharge and &lt;5% polymorphonuclear cells [PMN] in the cytologic sample), SE (clear or absent vaginal discharge and 5% PMN), or CE (vaginal mucus containingany signs of pus). The prevalence of SE and CE on Day 21 was 27.9% and 58.4%, respectively. Generally, samples from cows with SE and CE showed a greater bacterial growth density (BGD) than those from healthy cows. The BGD tended to be affected by the interaction of time by UHS (P ¼ 0.057). Differences between healthy, SE, and CE cows were found from Day 3 to the last sampling day. Furthermore, the percentage of PMN differed between healthy, SE, and CE cows and was affected by time in a cubic way (decrease/increase/decrease). Overall, E coli was found in 25.4% of the samples, and T pyogenes was identified in 30.2% of the samples. The risk for CE was increased by BGD and the presence of T pyogenes. Conversely, the presence of E coli had no effect on the risk of CE or the risk of SE. The risk for an infection with T pyogenes was greater in the first-parity cows and in cows with assisted calving. In conclusion, changes in BGD and proportion of PMN varied with the UHS (healthy, SE, and CE), which was affected by the presence of T pyogenes but not E coli.Facultad de Ciencias Veterinarias2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1316-1322http://sedici.unlp.edu.ar/handle/10915/159586enginfo:eu-repo/semantics/altIdentifier/issn/0093-691Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.theriogenology.2014.08.018info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:41:41Zoai:sedici.unlp.edu.ar:10915/159586Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:41:41.638SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Dynamics of bacteriological and cytological changes in the uterus of postpartum dairy cows
title Dynamics of bacteriological and cytological changes in the uterus of postpartum dairy cows
spellingShingle Dynamics of bacteriological and cytological changes in the uterus of postpartum dairy cows
Prunner, I.
Ciencias Veterinarias
Uterine disease
Bacteria
Cytology
Dairy cow
Fourier transform infrared spectroscopy
title_short Dynamics of bacteriological and cytological changes in the uterus of postpartum dairy cows
title_full Dynamics of bacteriological and cytological changes in the uterus of postpartum dairy cows
title_fullStr Dynamics of bacteriological and cytological changes in the uterus of postpartum dairy cows
title_full_unstemmed Dynamics of bacteriological and cytological changes in the uterus of postpartum dairy cows
title_sort Dynamics of bacteriological and cytological changes in the uterus of postpartum dairy cows
dc.creator.none.fl_str_mv Prunner, I.
Pothmann, H.
Wagener, K.
Giuliodori, Mauricio Javier
Huber, J.
Ehling Schulz, M.
Drillich, M.
author Prunner, I.
author_facet Prunner, I.
Pothmann, H.
Wagener, K.
Giuliodori, Mauricio Javier
Huber, J.
Ehling Schulz, M.
Drillich, M.
author_role author
author2 Pothmann, H.
Wagener, K.
Giuliodori, Mauricio Javier
Huber, J.
Ehling Schulz, M.
Drillich, M.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Uterine disease
Bacteria
Cytology
Dairy cow
Fourier transform infrared spectroscopy
topic Ciencias Veterinarias
Uterine disease
Bacteria
Cytology
Dairy cow
Fourier transform infrared spectroscopy
dc.description.none.fl_txt_mv The objectives of this study were to characterize clinical, intrauterine, bacteriologic and cytologic changes during the first month after parturition in healthy dairy cows and in cows with subclinical endometritis (SE) or clinical endometritis (CE). Furthermore, risk factors related to clinical bacteriologic and cytologic findings were determined. A total of 170 calvings were enrolled, and intrauterine samples were collected on Days 0, 3, 9, 15, 21, and 28 postpartum using the cytobrush technique. The presence of Escherichia coli and Trueperella pyogenes was determined by Fourier transform infrared spectroscopy. The cows were categorized according to their uterine health status (UHS) on Day 21 as healthy (clear or absent vaginal discharge and &lt;5% polymorphonuclear cells [PMN] in the cytologic sample), SE (clear or absent vaginal discharge and 5% PMN), or CE (vaginal mucus containingany signs of pus). The prevalence of SE and CE on Day 21 was 27.9% and 58.4%, respectively. Generally, samples from cows with SE and CE showed a greater bacterial growth density (BGD) than those from healthy cows. The BGD tended to be affected by the interaction of time by UHS (P ¼ 0.057). Differences between healthy, SE, and CE cows were found from Day 3 to the last sampling day. Furthermore, the percentage of PMN differed between healthy, SE, and CE cows and was affected by time in a cubic way (decrease/increase/decrease). Overall, E coli was found in 25.4% of the samples, and T pyogenes was identified in 30.2% of the samples. The risk for CE was increased by BGD and the presence of T pyogenes. Conversely, the presence of E coli had no effect on the risk of CE or the risk of SE. The risk for an infection with T pyogenes was greater in the first-parity cows and in cows with assisted calving. In conclusion, changes in BGD and proportion of PMN varied with the UHS (healthy, SE, and CE), which was affected by the presence of T pyogenes but not E coli.
Facultad de Ciencias Veterinarias
description The objectives of this study were to characterize clinical, intrauterine, bacteriologic and cytologic changes during the first month after parturition in healthy dairy cows and in cows with subclinical endometritis (SE) or clinical endometritis (CE). Furthermore, risk factors related to clinical bacteriologic and cytologic findings were determined. A total of 170 calvings were enrolled, and intrauterine samples were collected on Days 0, 3, 9, 15, 21, and 28 postpartum using the cytobrush technique. The presence of Escherichia coli and Trueperella pyogenes was determined by Fourier transform infrared spectroscopy. The cows were categorized according to their uterine health status (UHS) on Day 21 as healthy (clear or absent vaginal discharge and &lt;5% polymorphonuclear cells [PMN] in the cytologic sample), SE (clear or absent vaginal discharge and 5% PMN), or CE (vaginal mucus containingany signs of pus). The prevalence of SE and CE on Day 21 was 27.9% and 58.4%, respectively. Generally, samples from cows with SE and CE showed a greater bacterial growth density (BGD) than those from healthy cows. The BGD tended to be affected by the interaction of time by UHS (P ¼ 0.057). Differences between healthy, SE, and CE cows were found from Day 3 to the last sampling day. Furthermore, the percentage of PMN differed between healthy, SE, and CE cows and was affected by time in a cubic way (decrease/increase/decrease). Overall, E coli was found in 25.4% of the samples, and T pyogenes was identified in 30.2% of the samples. The risk for CE was increased by BGD and the presence of T pyogenes. Conversely, the presence of E coli had no effect on the risk of CE or the risk of SE. The risk for an infection with T pyogenes was greater in the first-parity cows and in cows with assisted calving. In conclusion, changes in BGD and proportion of PMN varied with the UHS (healthy, SE, and CE), which was affected by the presence of T pyogenes but not E coli.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
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dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/159586
url http://sedici.unlp.edu.ar/handle/10915/159586
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language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0093-691X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.theriogenology.2014.08.018
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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