<i>Nezara viridula</i> (Hemiptera: Pentatomidae) transcriptomic analysis and neuropeptidomics

Autores
Lavore, Andrés; Perez-Gianmarco, Lucila; Esponda Behrens, Natalia; Palacio, Victorio; Catalano, Maria Ines; Rivera Pomar, Rolando Víctor; Ons, Sheila
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Stinkbugs (Hemiptera: Pentatomidae) are of major economic importance as pest of crops. Among the species composing the stinkbug complex, Nezara viridula is one of the most abundant in Brazil, Argentina and the Southern USA. However, this species has been poorly characterized at the genetic and physiological level. Here we sequenced and analyzed the complete transcriptome of N. viridula male and female adults. We identifed neuropeptide precursor genes and G-protein coupled receptors for neuropeptides in this transcriptome. Mature neuropeptides were identifed in N. viridula brain extracts by liquid chromatography-tandem mass spectrometry. We also analyzed the neuropeptide precursor complement in the genome sequence of Halyomorpha halys, another pentatomid of economic relevance. We compared the results in both pentatomids with the well-characterized neuropeptide repertoire from the kissing bug Rhodnius prolixus (Hemiptera: Reduviidae). We identifed both groupspecifc features (which could be related to the diferent feeding habits) and similarities that could be characteristic of Heteroptera. This work contributes to a deeper knowledge of the genetic information of these pests, with a focus on neuroendocrine system characterization.
Centro Regional de Estudios Genómicos
Materia
Ciencias Exactas
Viridula
Neuropeptide
Precursor Gene
Pentatomid
Stinkbug
Prolixus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/107344
Acceder
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network_name_str SEDICI (UNLP)
spelling <i>Nezara viridula</i> (Hemiptera: Pentatomidae) transcriptomic analysis and neuropeptidomicsLavore, AndrésPerez-Gianmarco, LucilaEsponda Behrens, NataliaPalacio, VictorioCatalano, Maria InesRivera Pomar, Rolando VíctorOns, SheilaCiencias ExactasViridulaNeuropeptidePrecursor GenePentatomidStinkbugProlixusStinkbugs (Hemiptera: Pentatomidae) are of major economic importance as pest of crops. Among the species composing the stinkbug complex, Nezara viridula is one of the most abundant in Brazil, Argentina and the Southern USA. However, this species has been poorly characterized at the genetic and physiological level. Here we sequenced and analyzed the complete transcriptome of N. viridula male and female adults. We identifed neuropeptide precursor genes and G-protein coupled receptors for neuropeptides in this transcriptome. Mature neuropeptides were identifed in N. viridula brain extracts by liquid chromatography-tandem mass spectrometry. We also analyzed the neuropeptide precursor complement in the genome sequence of Halyomorpha halys, another pentatomid of economic relevance. We compared the results in both pentatomids with the well-characterized neuropeptide repertoire from the kissing bug Rhodnius prolixus (Hemiptera: Reduviidae). We identifed both groupspecifc features (which could be related to the diferent feeding habits) and similarities that could be characteristic of Heteroptera. This work contributes to a deeper knowledge of the genetic information of these pests, with a focus on neuroendocrine system characterization.Centro Regional de Estudios Genómicos2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/107344enginfo:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6250713&blobtype=pdfinfo:eu-repo/semantics/altIdentifier/issn/2045-2322info:eu-repo/semantics/altIdentifier/pmid/30467353info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-35386-4info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:56:06Zoai:sedici.unlp.edu.ar:10915/107344Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:56:06.84SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv <i>Nezara viridula</i> (Hemiptera: Pentatomidae) transcriptomic analysis and neuropeptidomics
title <i>Nezara viridula</i> (Hemiptera: Pentatomidae) transcriptomic analysis and neuropeptidomics
spellingShingle <i>Nezara viridula</i> (Hemiptera: Pentatomidae) transcriptomic analysis and neuropeptidomics
Lavore, Andrés
Ciencias Exactas
Viridula
Neuropeptide
Precursor Gene
Pentatomid
Stinkbug
Prolixus
title_short <i>Nezara viridula</i> (Hemiptera: Pentatomidae) transcriptomic analysis and neuropeptidomics
title_full <i>Nezara viridula</i> (Hemiptera: Pentatomidae) transcriptomic analysis and neuropeptidomics
title_fullStr <i>Nezara viridula</i> (Hemiptera: Pentatomidae) transcriptomic analysis and neuropeptidomics
title_full_unstemmed <i>Nezara viridula</i> (Hemiptera: Pentatomidae) transcriptomic analysis and neuropeptidomics
title_sort <i>Nezara viridula</i> (Hemiptera: Pentatomidae) transcriptomic analysis and neuropeptidomics
dc.creator.none.fl_str_mv Lavore, Andrés
Perez-Gianmarco, Lucila
Esponda Behrens, Natalia
Palacio, Victorio
Catalano, Maria Ines
Rivera Pomar, Rolando Víctor
Ons, Sheila
author Lavore, Andrés
author_facet Lavore, Andrés
Perez-Gianmarco, Lucila
Esponda Behrens, Natalia
Palacio, Victorio
Catalano, Maria Ines
Rivera Pomar, Rolando Víctor
Ons, Sheila
author_role author
author2 Perez-Gianmarco, Lucila
Esponda Behrens, Natalia
Palacio, Victorio
Catalano, Maria Ines
Rivera Pomar, Rolando Víctor
Ons, Sheila
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Viridula
Neuropeptide
Precursor Gene
Pentatomid
Stinkbug
Prolixus
topic Ciencias Exactas
Viridula
Neuropeptide
Precursor Gene
Pentatomid
Stinkbug
Prolixus
dc.description.none.fl_txt_mv Stinkbugs (Hemiptera: Pentatomidae) are of major economic importance as pest of crops. Among the species composing the stinkbug complex, Nezara viridula is one of the most abundant in Brazil, Argentina and the Southern USA. However, this species has been poorly characterized at the genetic and physiological level. Here we sequenced and analyzed the complete transcriptome of N. viridula male and female adults. We identifed neuropeptide precursor genes and G-protein coupled receptors for neuropeptides in this transcriptome. Mature neuropeptides were identifed in N. viridula brain extracts by liquid chromatography-tandem mass spectrometry. We also analyzed the neuropeptide precursor complement in the genome sequence of Halyomorpha halys, another pentatomid of economic relevance. We compared the results in both pentatomids with the well-characterized neuropeptide repertoire from the kissing bug Rhodnius prolixus (Hemiptera: Reduviidae). We identifed both groupspecifc features (which could be related to the diferent feeding habits) and similarities that could be characteristic of Heteroptera. This work contributes to a deeper knowledge of the genetic information of these pests, with a focus on neuroendocrine system characterization.
Centro Regional de Estudios Genómicos
description Stinkbugs (Hemiptera: Pentatomidae) are of major economic importance as pest of crops. Among the species composing the stinkbug complex, Nezara viridula is one of the most abundant in Brazil, Argentina and the Southern USA. However, this species has been poorly characterized at the genetic and physiological level. Here we sequenced and analyzed the complete transcriptome of N. viridula male and female adults. We identifed neuropeptide precursor genes and G-protein coupled receptors for neuropeptides in this transcriptome. Mature neuropeptides were identifed in N. viridula brain extracts by liquid chromatography-tandem mass spectrometry. We also analyzed the neuropeptide precursor complement in the genome sequence of Halyomorpha halys, another pentatomid of economic relevance. We compared the results in both pentatomids with the well-characterized neuropeptide repertoire from the kissing bug Rhodnius prolixus (Hemiptera: Reduviidae). We identifed both groupspecifc features (which could be related to the diferent feeding habits) and similarities that could be characteristic of Heteroptera. This work contributes to a deeper knowledge of the genetic information of these pests, with a focus on neuroendocrine system characterization.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/107344
url http://sedici.unlp.edu.ar/handle/10915/107344
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6250713&blobtype=pdf
info:eu-repo/semantics/altIdentifier/issn/2045-2322
info:eu-repo/semantics/altIdentifier/pmid/30467353
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-35386-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.13397

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