ISSR markers to explore entomopathogenic fungi genetic diversity: Implications for biological control of tobacco pests

Autores
Vianna, María Florencia; Pelizza, Sebastián Alberto; Russo, María Leticia; Toledo, Andrea Vanesa; Mourelos, Cecilia Alejandra; Scorsetti, Ana Clara
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Tobacco is one of the major industrial crops cultivated worldwide. Chemical control is the main method employed to reduce damage by insect pests. The use of entomopathogenic fungi represents an alternative to replace insecticides. The search for effective strains in the field constitutes a first step when developing a formulation. The objective of this work was to study genetic differences among isolates of entomopathogenic fungi obtained from tobacco grown soils using ISSR markers. The pathogenicity of the strains towards Helicoverpa gelotopoeon and Diabrotica speciosa was also assessed in order to search for a relationship between virulence and genetic diversity. Nineteen isolates were identified according to morphological features and molecular techniques as Beauveria bassiana (11) and Purpureocillium lilacinum (8). The diversity tree generated by ISSR analysis showed a high diversity among the strains. The pathogenicity towards H. gelotopoeon and D. speciosa was assessed and the logistic models generated showed that B. bassiana isolates LPSc1215 and LPSc1364 were the most pathogenic against both insect pests tested. In the diversity tree, these strains were grouped in a same cluster with a similarity level of approximately 85%, indicating a possible relationship between virulence and the band pattern generated.
Facultad de Ciencias Naturales y Museo
Instituto de Botánica "Dr. Carlos Spegazzini"
Facultad de Ciencias Agrarias y Forestales
Centro de Investigaciones en Fitopatología
Materia
Ciencias Naturales
Ciencias Agrarias
Beauveria bassiana
Diabrotica speciose
genetic polymorphism
Helicoverpa gelotopoeon
pathogenicity
Purpureocillium lilacinum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/130867
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/130867
network_acronym_str SEDICI
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network_name_str SEDICI (UNLP)
spelling ISSR markers to explore entomopathogenic fungi genetic diversity: Implications for biological control of tobacco pestsVianna, María FlorenciaPelizza, Sebastián AlbertoRusso, María LeticiaToledo, Andrea VanesaMourelos, Cecilia AlejandraScorsetti, Ana ClaraCiencias NaturalesCiencias AgrariasBeauveria bassianaDiabrotica speciosegenetic polymorphismHelicoverpa gelotopoeonpathogenicityPurpureocillium lilacinumTobacco is one of the major industrial crops cultivated worldwide. Chemical control is the main method employed to reduce damage by insect pests. The use of entomopathogenic fungi represents an alternative to replace insecticides. The search for effective strains in the field constitutes a first step when developing a formulation. The objective of this work was to study genetic differences among isolates of entomopathogenic fungi obtained from tobacco grown soils using ISSR markers. The pathogenicity of the strains towards <i>Helicoverpa gelotopoeon</i> and <i>Diabrotica speciosa</i> was also assessed in order to search for a relationship between virulence and genetic diversity. Nineteen isolates were identified according to morphological features and molecular techniques as <i>Beauveria bassiana</i> (11) and <i>Purpureocillium lilacinum</i> (8). The diversity tree generated by ISSR analysis showed a high diversity among the strains. The pathogenicity towards <i>H. gelotopoeon</i> and <i>D. speciosa</i> was assessed and the logistic models generated showed that <i>B. bassiana</i> isolates LPSc1215 and LPSc1364 were the most pathogenic against both insect pests tested. In the diversity tree, these strains were grouped in a same cluster with a similarity level of approximately 85%, indicating a possible relationship between virulence and the band pattern generated.Facultad de Ciencias Naturales y MuseoInstituto de Botánica "Dr. Carlos Spegazzini"Facultad de Ciencias Agrarias y ForestalesCentro de Investigaciones en Fitopatología2020-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/130867enginfo:eu-repo/semantics/altIdentifier/issn/0973-7138info:eu-repo/semantics/altIdentifier/issn/0250-5991info:eu-repo/semantics/altIdentifier/doi/10.1007/s12038-020-00108-4info:eu-repo/semantics/altIdentifier/pmid/33361627info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:31:42Zoai:sedici.unlp.edu.ar:10915/130867Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:31:42.575SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv ISSR markers to explore entomopathogenic fungi genetic diversity: Implications for biological control of tobacco pests
title ISSR markers to explore entomopathogenic fungi genetic diversity: Implications for biological control of tobacco pests
spellingShingle ISSR markers to explore entomopathogenic fungi genetic diversity: Implications for biological control of tobacco pests
Vianna, María Florencia
Ciencias Naturales
Ciencias Agrarias
Beauveria bassiana
Diabrotica speciose
genetic polymorphism
Helicoverpa gelotopoeon
pathogenicity
Purpureocillium lilacinum
title_short ISSR markers to explore entomopathogenic fungi genetic diversity: Implications for biological control of tobacco pests
title_full ISSR markers to explore entomopathogenic fungi genetic diversity: Implications for biological control of tobacco pests
title_fullStr ISSR markers to explore entomopathogenic fungi genetic diversity: Implications for biological control of tobacco pests
title_full_unstemmed ISSR markers to explore entomopathogenic fungi genetic diversity: Implications for biological control of tobacco pests
title_sort ISSR markers to explore entomopathogenic fungi genetic diversity: Implications for biological control of tobacco pests
dc.creator.none.fl_str_mv Vianna, María Florencia
Pelizza, Sebastián Alberto
Russo, María Leticia
Toledo, Andrea Vanesa
Mourelos, Cecilia Alejandra
Scorsetti, Ana Clara
author Vianna, María Florencia
author_facet Vianna, María Florencia
Pelizza, Sebastián Alberto
Russo, María Leticia
Toledo, Andrea Vanesa
Mourelos, Cecilia Alejandra
Scorsetti, Ana Clara
author_role author
author2 Pelizza, Sebastián Alberto
Russo, María Leticia
Toledo, Andrea Vanesa
Mourelos, Cecilia Alejandra
Scorsetti, Ana Clara
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Naturales
Ciencias Agrarias
Beauveria bassiana
Diabrotica speciose
genetic polymorphism
Helicoverpa gelotopoeon
pathogenicity
Purpureocillium lilacinum
topic Ciencias Naturales
Ciencias Agrarias
Beauveria bassiana
Diabrotica speciose
genetic polymorphism
Helicoverpa gelotopoeon
pathogenicity
Purpureocillium lilacinum
dc.description.none.fl_txt_mv Tobacco is one of the major industrial crops cultivated worldwide. Chemical control is the main method employed to reduce damage by insect pests. The use of entomopathogenic fungi represents an alternative to replace insecticides. The search for effective strains in the field constitutes a first step when developing a formulation. The objective of this work was to study genetic differences among isolates of entomopathogenic fungi obtained from tobacco grown soils using ISSR markers. The pathogenicity of the strains towards <i>Helicoverpa gelotopoeon</i> and <i>Diabrotica speciosa</i> was also assessed in order to search for a relationship between virulence and genetic diversity. Nineteen isolates were identified according to morphological features and molecular techniques as <i>Beauveria bassiana</i> (11) and <i>Purpureocillium lilacinum</i> (8). The diversity tree generated by ISSR analysis showed a high diversity among the strains. The pathogenicity towards <i>H. gelotopoeon</i> and <i>D. speciosa</i> was assessed and the logistic models generated showed that <i>B. bassiana</i> isolates LPSc1215 and LPSc1364 were the most pathogenic against both insect pests tested. In the diversity tree, these strains were grouped in a same cluster with a similarity level of approximately 85%, indicating a possible relationship between virulence and the band pattern generated.
Facultad de Ciencias Naturales y Museo
Instituto de Botánica "Dr. Carlos Spegazzini"
Facultad de Ciencias Agrarias y Forestales
Centro de Investigaciones en Fitopatología
description Tobacco is one of the major industrial crops cultivated worldwide. Chemical control is the main method employed to reduce damage by insect pests. The use of entomopathogenic fungi represents an alternative to replace insecticides. The search for effective strains in the field constitutes a first step when developing a formulation. The objective of this work was to study genetic differences among isolates of entomopathogenic fungi obtained from tobacco grown soils using ISSR markers. The pathogenicity of the strains towards <i>Helicoverpa gelotopoeon</i> and <i>Diabrotica speciosa</i> was also assessed in order to search for a relationship between virulence and genetic diversity. Nineteen isolates were identified according to morphological features and molecular techniques as <i>Beauveria bassiana</i> (11) and <i>Purpureocillium lilacinum</i> (8). The diversity tree generated by ISSR analysis showed a high diversity among the strains. The pathogenicity towards <i>H. gelotopoeon</i> and <i>D. speciosa</i> was assessed and the logistic models generated showed that <i>B. bassiana</i> isolates LPSc1215 and LPSc1364 were the most pathogenic against both insect pests tested. In the diversity tree, these strains were grouped in a same cluster with a similarity level of approximately 85%, indicating a possible relationship between virulence and the band pattern generated.
publishDate 2020
dc.date.none.fl_str_mv 2020-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/130867
url http://sedici.unlp.edu.ar/handle/10915/130867
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0973-7138
info:eu-repo/semantics/altIdentifier/issn/0250-5991
info:eu-repo/semantics/altIdentifier/doi/10.1007/s12038-020-00108-4
info:eu-repo/semantics/altIdentifier/pmid/33361627
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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