Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in paspalum notatum

Autores
Podio, Maricel; Rodríguez, María Pía; Felitti, Silvina Andrea; Stein, Juliana; Martínez, Eric Javier; Siena, Lorena Adelina; Quarin, Camilo Luis; Pessino, Silvina Claudia; Ortíz, Juan Pablo Amelio
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Podio, Maricel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Podio, Maricel. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias, Argentina.
Fil. Rodríguez, María Pía. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias, Argentina.
Fil: Felitti, Silvina Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias, Argentina.
Fil: Stein, Juliana. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias, Argentina.
Fil: Martínez, Eric J. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Siena, Lorena Adelina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias, Argentina.
Fil: Quarin, Camilo Luis. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias, Argentina.
Fil: Ortíz, Juan Pablo Amelio. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias, Argentina.
In previous studies we reported the identification of several AFLP, RAPD and RFLP molecular markers linked to apospory in Paspalum notatum. The objective of this work was to sequence these markers, obtain their flanking regions by chromosome walking and perform an in silico mapping analysis in rice and maize. The methylation status of two apospory-related sequences was also assessed using methylation-sensitive RFLP experiments. Fourteen molecular markers were analyzed and several protein-coding sequences were identified. Copy number estimates and RFLP linkage analysis showed that the sequence PnMAI3 displayed 2-4 copies per genome and linkage to apospory. Extension of this marker by chromosome walking revealed an additional protein-coding sequence mapping in silico in the apospory-syntenic regions of rice and maize. Approximately 5 kb corresponding to different markers were characterized through the global sequencing procedure. A more refined analysis based on sequence information indicated synteny with segments of chromosomes 2 and 12 of rice and chromosomes 3 and 5 of maize. Two loci associated with apomixis locus were tested in methylation-sensitive RFLP experiments using genomic DNA extracted from leaves. Although both target sequences were methylated no methylation polymorphisms associated with the mode of reproduction were detected.
Fuente
Genetics and Molecular Biology, 2012, vol. 35, no. 4, p. 827-837.
Materia
Apomixis
Chromosome walking
Gene mapping
Molecular markers
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/33815
Acceder
id RIUNNE_69a89e90b4a095d037c8c12ce92926ea
oai_identifier_str oai:repositorio.unne.edu.ar:123456789/33815
network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in paspalum notatumPodio, MaricelRodríguez, María PíaFelitti, Silvina AndreaStein, JulianaMartínez, Eric JavierSiena, Lorena AdelinaQuarin, Camilo LuisPessino, Silvina ClaudiaOrtíz, Juan Pablo AmelioApomixisChromosome walkingGene mappingMolecular markersFil: Podio, Maricel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Podio, Maricel. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias, Argentina.Fil. Rodríguez, María Pía. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias, Argentina.Fil: Felitti, Silvina Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias, Argentina.Fil: Stein, Juliana. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias, Argentina.Fil: Martínez, Eric J. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Siena, Lorena Adelina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias, Argentina.Fil: Quarin, Camilo Luis. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias, Argentina.Fil: Ortíz, Juan Pablo Amelio. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias, Argentina.In previous studies we reported the identification of several AFLP, RAPD and RFLP molecular markers linked to apospory in Paspalum notatum. The objective of this work was to sequence these markers, obtain their flanking regions by chromosome walking and perform an in silico mapping analysis in rice and maize. The methylation status of two apospory-related sequences was also assessed using methylation-sensitive RFLP experiments. Fourteen molecular markers were analyzed and several protein-coding sequences were identified. Copy number estimates and RFLP linkage analysis showed that the sequence PnMAI3 displayed 2-4 copies per genome and linkage to apospory. Extension of this marker by chromosome walking revealed an additional protein-coding sequence mapping in silico in the apospory-syntenic regions of rice and maize. Approximately 5 kb corresponding to different markers were characterized through the global sequencing procedure. A more refined analysis based on sequence information indicated synteny with segments of chromosomes 2 and 12 of rice and chromosomes 3 and 5 of maize. Two loci associated with apomixis locus were tested in methylation-sensitive RFLP experiments using genomic DNA extracted from leaves. Although both target sequences were methylated no methylation polymorphisms associated with the mode of reproduction were detected.Sociedade Brasileira de Genética2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfPodio, Maricel, et al., 2012. Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in paspalum notatum. Genetics and Molecular Biology. Ribeirão Preto: Sociedade Brasileira de Genética, vol. 35, no. 4, p. 827-837. ISSN 1678-4685.0100-8455http://repositorio.unne.edu.ar/handle/123456789/33815Genetics and Molecular Biology, 2012, vol. 35, no. 4, p. 827-837.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenghttps://www.scielo.br/j/gmb/a/SZJB4G9dwpP5hzHSmD9gfyg/?lang=eninfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-11-06T10:09:02Zoai:repositorio.unne.edu.ar:123456789/33815instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-11-06 10:09:02.668Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in paspalum notatum
title Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in paspalum notatum
spellingShingle Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in paspalum notatum
Podio, Maricel
Apomixis
Chromosome walking
Gene mapping
Molecular markers
title_short Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in paspalum notatum
title_full Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in paspalum notatum
title_fullStr Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in paspalum notatum
title_full_unstemmed Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in paspalum notatum
title_sort Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in paspalum notatum
dc.creator.none.fl_str_mv Podio, Maricel
Rodríguez, María Pía
Felitti, Silvina Andrea
Stein, Juliana
Martínez, Eric Javier
Siena, Lorena Adelina
Quarin, Camilo Luis
Pessino, Silvina Claudia
Ortíz, Juan Pablo Amelio
author Podio, Maricel
author_facet Podio, Maricel
Rodríguez, María Pía
Felitti, Silvina Andrea
Stein, Juliana
Martínez, Eric Javier
Siena, Lorena Adelina
Quarin, Camilo Luis
Pessino, Silvina Claudia
Ortíz, Juan Pablo Amelio
author_role author
author2 Rodríguez, María Pía
Felitti, Silvina Andrea
Stein, Juliana
Martínez, Eric Javier
Siena, Lorena Adelina
Quarin, Camilo Luis
Pessino, Silvina Claudia
Ortíz, Juan Pablo Amelio
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Apomixis
Chromosome walking
Gene mapping
Molecular markers
topic Apomixis
Chromosome walking
Gene mapping
Molecular markers
dc.description.none.fl_txt_mv Fil: Podio, Maricel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Podio, Maricel. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias, Argentina.
Fil. Rodríguez, María Pía. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias, Argentina.
Fil: Felitti, Silvina Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias, Argentina.
Fil: Stein, Juliana. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias, Argentina.
Fil: Martínez, Eric J. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Siena, Lorena Adelina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias, Argentina.
Fil: Quarin, Camilo Luis. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias, Argentina.
Fil: Ortíz, Juan Pablo Amelio. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias, Argentina.
In previous studies we reported the identification of several AFLP, RAPD and RFLP molecular markers linked to apospory in Paspalum notatum. The objective of this work was to sequence these markers, obtain their flanking regions by chromosome walking and perform an in silico mapping analysis in rice and maize. The methylation status of two apospory-related sequences was also assessed using methylation-sensitive RFLP experiments. Fourteen molecular markers were analyzed and several protein-coding sequences were identified. Copy number estimates and RFLP linkage analysis showed that the sequence PnMAI3 displayed 2-4 copies per genome and linkage to apospory. Extension of this marker by chromosome walking revealed an additional protein-coding sequence mapping in silico in the apospory-syntenic regions of rice and maize. Approximately 5 kb corresponding to different markers were characterized through the global sequencing procedure. A more refined analysis based on sequence information indicated synteny with segments of chromosomes 2 and 12 of rice and chromosomes 3 and 5 of maize. Two loci associated with apomixis locus were tested in methylation-sensitive RFLP experiments using genomic DNA extracted from leaves. Although both target sequences were methylated no methylation polymorphisms associated with the mode of reproduction were detected.
description Fil: Podio, Maricel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Podio, Maricel, et al., 2012. Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in paspalum notatum. Genetics and Molecular Biology. Ribeirão Preto: Sociedade Brasileira de Genética, vol. 35, no. 4, p. 827-837. ISSN 1678-4685.
0100-8455
http://repositorio.unne.edu.ar/handle/123456789/33815
identifier_str_mv Podio, Maricel, et al., 2012. Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in paspalum notatum. Genetics and Molecular Biology. Ribeirão Preto: Sociedade Brasileira de Genética, vol. 35, no. 4, p. 827-837. ISSN 1678-4685.
0100-8455
url http://repositorio.unne.edu.ar/handle/123456789/33815
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.scielo.br/j/gmb/a/SZJB4G9dwpP5hzHSmD9gfyg/?lang=en
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology, 2012, vol. 35, no. 4, p. 827-837.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
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score 12.976206

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