Corrigendum to “CpG-ODN formulated with a nanostructure as adjuvant for anticrotalic serum production. Studies in mice” [Toxicon 215 (2022) 28–36]

Autores
Fusco, Luciano S.; Pascual, María M.; Hernandez, David; Sánchez Vallecillo, María F.; Arrieta, María B.; Moron, Gabriel; Palma, Santiago; Maletto, Belkys A.; Leiva, Laura C.
Año de publicación
2022
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Fusco, Luciano S. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.
Fil: Fusco, Luciano S. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
Fil: Pascual, María M. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina.
Fil: Pascual, María M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina.
Fil: Hernandez, David. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Sánchez Vallecillo, María F. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina.
Fil: Sánchez Vallecillo, María F. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina.
Fil: Arrieta, María B. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura; Argentina.
Fil: Moron, Gabriel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina.
Fil: Moron, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina.
Fil: Palma, Santiago. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina.
Fil: Palma, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina.
Fil: Maletto, Belkys A. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina.
Fil: Maletto, Belkys A. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina.
Fil: Leiva, Laura C. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.
Fil: Fil: Leiva, Laura C. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura.; Argentina.
Antivenom is the only safe and effective treatment to neutralize snake venom. Specific anti-venom used to treat snake bite is usually obtained from horses after hyperimmunization with crude snake venom in combination with Freund’s Adjuvant. Freund’s complete and incomplete adjuvant can cause severe local and sys- temic acute and chronic inflammation, its potentially severe inflammatory effects have led many researchers to seek alternative immunological adjuvants. CpG-ODN formulated in a 6-O-ascorbyl palmitate nanostructure (Coa-ASC16) was more efficient as adjuvant than CpG-ODN alone using ovalbumin (OVA) as an antigen model. Particularly, immunization of mice with OVA/CpG-ODN/Coa-ASC16 resulted in high OVA specific antibody titers and IFN-γ and IL-17 secretion compared to im- munization with OVA/CpG-ODN. First of all, we estimated the effect of Coa-ASC16 nanostructure preparation on venom activity. Additionally, in order to evaluate the immune response induced by this adjuvant strategy using Crotalus durissus terrificus (C. d. terrificus) venom (CdtV), we determined the titer of antibodies (IgG, IgG1 and IgG2) and their specificity. BALB/c mice were subcutaneously immunizated on days 0, 15 and 30 with CdtV/CpG-ODN/Coa-ASC16 or CdtV/Freund’s Adyuvant (complete first and incomplete-booster) (dose/mice: CdtV: 6–10 μg, CpG-ODN: 30 μg). On day 45 mice were sacrificed. The neutralizing ability of serum from animals immunized with CdtV/CpG-ODN/Coa-ASC16 or CdtV/Freund’s adjuvant was tested against PLA2 activity and lethality. In both immunized group mice, the antibody titers in plasma were high (1 × 105 ), with a similar IgG1/IgG2a ratio. The antibodies recognized phospholipase A2 and thrombin-like proteins, the main toxins from C. d. terrificus venom. Macroscopic and microscopic analysis at the site of injection of mice injected with Freund’s adjuvant showed local damage (with non-infectious abscesses) and hypertrophy of inguinal lymph nodes, whereas mice injected with CpG-ODN/Coa/ASC16 did not. Our results show that CpG-ODN/Coa- ASC16 produces a humoral response as strong and specific as Freund’s adjuvant, with minor or null local deleterious effect, demonstrating the potentiality and advisability of an alternative formulation as a new adjuvant option for future immunizations to produce C. d. terrificus antivenom.
Fuente
Toxicon, 2022, vol. 216, p. 114.
Materia
CpG-ODN
Snake venom
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/58048
Acceder
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network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Corrigendum to “CpG-ODN formulated with a nanostructure as adjuvant for anticrotalic serum production. Studies in mice” [Toxicon 215 (2022) 28–36]Fusco, Luciano S.Pascual, María M.Hernandez, DavidSánchez Vallecillo, María F.Arrieta, María B.Moron, GabrielPalma, SantiagoMaletto, Belkys A.Leiva, Laura C.CpG-ODNSnake venomFil: Fusco, Luciano S. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.Fil: Fusco, Luciano S. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.Fil: Pascual, María M. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina.Fil: Pascual, María M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina.Fil: Hernandez, David. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.Fil: Sánchez Vallecillo, María F. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina.Fil: Sánchez Vallecillo, María F. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina.Fil: Arrieta, María B. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura; Argentina.Fil: Moron, Gabriel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina.Fil: Moron, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina.Fil: Palma, Santiago. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina.Fil: Palma, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina.Fil: Maletto, Belkys A. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina.Fil: Maletto, Belkys A. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina.Fil: Leiva, Laura C. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.Fil: Fil: Leiva, Laura C. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura.; Argentina.Antivenom is the only safe and effective treatment to neutralize snake venom. Specific anti-venom used to treat snake bite is usually obtained from horses after hyperimmunization with crude snake venom in combination with Freund’s Adjuvant. Freund’s complete and incomplete adjuvant can cause severe local and sys- temic acute and chronic inflammation, its potentially severe inflammatory effects have led many researchers to seek alternative immunological adjuvants. CpG-ODN formulated in a 6-O-ascorbyl palmitate nanostructure (Coa-ASC16) was more efficient as adjuvant than CpG-ODN alone using ovalbumin (OVA) as an antigen model. Particularly, immunization of mice with OVA/CpG-ODN/Coa-ASC16 resulted in high OVA specific antibody titers and IFN-γ and IL-17 secretion compared to im- munization with OVA/CpG-ODN. First of all, we estimated the effect of Coa-ASC16 nanostructure preparation on venom activity. Additionally, in order to evaluate the immune response induced by this adjuvant strategy using Crotalus durissus terrificus (C. d. terrificus) venom (CdtV), we determined the titer of antibodies (IgG, IgG1 and IgG2) and their specificity. BALB/c mice were subcutaneously immunizated on days 0, 15 and 30 with CdtV/CpG-ODN/Coa-ASC16 or CdtV/Freund’s Adyuvant (complete first and incomplete-booster) (dose/mice: CdtV: 6–10 μg, CpG-ODN: 30 μg). On day 45 mice were sacrificed. The neutralizing ability of serum from animals immunized with CdtV/CpG-ODN/Coa-ASC16 or CdtV/Freund’s adjuvant was tested against PLA2 activity and lethality. In both immunized group mice, the antibody titers in plasma were high (1 × 105 ), with a similar IgG1/IgG2a ratio. The antibodies recognized phospholipase A2 and thrombin-like proteins, the main toxins from C. d. terrificus venom. Macroscopic and microscopic analysis at the site of injection of mice injected with Freund’s adjuvant showed local damage (with non-infectious abscesses) and hypertrophy of inguinal lymph nodes, whereas mice injected with CpG-ODN/Coa/ASC16 did not. Our results show that CpG-ODN/Coa- ASC16 produces a humoral response as strong and specific as Freund’s adjuvant, with minor or null local deleterious effect, demonstrating the potentiality and advisability of an alternative formulation as a new adjuvant option for future immunizations to produce C. d. terrificus antivenom.Elsevier Ltd.2022-09-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfp. 1-1application/pdfFusco, Luciano S., et. al, 2022. Corrigendum to “CpG-ODN formulated with a nanostructure as adjuvant for anticrotalic serum production. Studies in mice” [Toxicon 215 (2022) 28–36]. Toxicon: Países BajosElsevier Ltd., p. 1-1, ISSN 0041-0101.0041-0101http://repositorio.unne.edu.ar/handle/123456789/58048Toxicon, 2022, vol. 216, p. 114.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordestespahttps://doi.org/10.1016/j.toxicon.2022.07.002https://doi.org/10.1016/j.toxicon.2022.05.045info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-09-29T14:30:49Zoai:repositorio.unne.edu.ar:123456789/58048instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-09-29 14:30:50.011Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Corrigendum to “CpG-ODN formulated with a nanostructure as adjuvant for anticrotalic serum production. Studies in mice” [Toxicon 215 (2022) 28–36]
title Corrigendum to “CpG-ODN formulated with a nanostructure as adjuvant for anticrotalic serum production. Studies in mice” [Toxicon 215 (2022) 28–36]
spellingShingle Corrigendum to “CpG-ODN formulated with a nanostructure as adjuvant for anticrotalic serum production. Studies in mice” [Toxicon 215 (2022) 28–36]
Fusco, Luciano S.
CpG-ODN
Snake venom
title_short Corrigendum to “CpG-ODN formulated with a nanostructure as adjuvant for anticrotalic serum production. Studies in mice” [Toxicon 215 (2022) 28–36]
title_full Corrigendum to “CpG-ODN formulated with a nanostructure as adjuvant for anticrotalic serum production. Studies in mice” [Toxicon 215 (2022) 28–36]
title_fullStr Corrigendum to “CpG-ODN formulated with a nanostructure as adjuvant for anticrotalic serum production. Studies in mice” [Toxicon 215 (2022) 28–36]
title_full_unstemmed Corrigendum to “CpG-ODN formulated with a nanostructure as adjuvant for anticrotalic serum production. Studies in mice” [Toxicon 215 (2022) 28–36]
title_sort Corrigendum to “CpG-ODN formulated with a nanostructure as adjuvant for anticrotalic serum production. Studies in mice” [Toxicon 215 (2022) 28–36]
dc.creator.none.fl_str_mv Fusco, Luciano S.
Pascual, María M.
Hernandez, David
Sánchez Vallecillo, María F.
Arrieta, María B.
Moron, Gabriel
Palma, Santiago
Maletto, Belkys A.
Leiva, Laura C.
author Fusco, Luciano S.
author_facet Fusco, Luciano S.
Pascual, María M.
Hernandez, David
Sánchez Vallecillo, María F.
Arrieta, María B.
Moron, Gabriel
Palma, Santiago
Maletto, Belkys A.
Leiva, Laura C.
author_role author
author2 Pascual, María M.
Hernandez, David
Sánchez Vallecillo, María F.
Arrieta, María B.
Moron, Gabriel
Palma, Santiago
Maletto, Belkys A.
Leiva, Laura C.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CpG-ODN
Snake venom
topic CpG-ODN
Snake venom
dc.description.none.fl_txt_mv Fil: Fusco, Luciano S. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.
Fil: Fusco, Luciano S. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
Fil: Pascual, María M. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina.
Fil: Pascual, María M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina.
Fil: Hernandez, David. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Sánchez Vallecillo, María F. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina.
Fil: Sánchez Vallecillo, María F. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina.
Fil: Arrieta, María B. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura; Argentina.
Fil: Moron, Gabriel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina.
Fil: Moron, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina.
Fil: Palma, Santiago. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina.
Fil: Palma, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina.
Fil: Maletto, Belkys A. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina.
Fil: Maletto, Belkys A. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina.
Fil: Leiva, Laura C. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.
Fil: Fil: Leiva, Laura C. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura.; Argentina.
Antivenom is the only safe and effective treatment to neutralize snake venom. Specific anti-venom used to treat snake bite is usually obtained from horses after hyperimmunization with crude snake venom in combination with Freund’s Adjuvant. Freund’s complete and incomplete adjuvant can cause severe local and sys- temic acute and chronic inflammation, its potentially severe inflammatory effects have led many researchers to seek alternative immunological adjuvants. CpG-ODN formulated in a 6-O-ascorbyl palmitate nanostructure (Coa-ASC16) was more efficient as adjuvant than CpG-ODN alone using ovalbumin (OVA) as an antigen model. Particularly, immunization of mice with OVA/CpG-ODN/Coa-ASC16 resulted in high OVA specific antibody titers and IFN-γ and IL-17 secretion compared to im- munization with OVA/CpG-ODN. First of all, we estimated the effect of Coa-ASC16 nanostructure preparation on venom activity. Additionally, in order to evaluate the immune response induced by this adjuvant strategy using Crotalus durissus terrificus (C. d. terrificus) venom (CdtV), we determined the titer of antibodies (IgG, IgG1 and IgG2) and their specificity. BALB/c mice were subcutaneously immunizated on days 0, 15 and 30 with CdtV/CpG-ODN/Coa-ASC16 or CdtV/Freund’s Adyuvant (complete first and incomplete-booster) (dose/mice: CdtV: 6–10 μg, CpG-ODN: 30 μg). On day 45 mice were sacrificed. The neutralizing ability of serum from animals immunized with CdtV/CpG-ODN/Coa-ASC16 or CdtV/Freund’s adjuvant was tested against PLA2 activity and lethality. In both immunized group mice, the antibody titers in plasma were high (1 × 105 ), with a similar IgG1/IgG2a ratio. The antibodies recognized phospholipase A2 and thrombin-like proteins, the main toxins from C. d. terrificus venom. Macroscopic and microscopic analysis at the site of injection of mice injected with Freund’s adjuvant showed local damage (with non-infectious abscesses) and hypertrophy of inguinal lymph nodes, whereas mice injected with CpG-ODN/Coa/ASC16 did not. Our results show that CpG-ODN/Coa- ASC16 produces a humoral response as strong and specific as Freund’s adjuvant, with minor or null local deleterious effect, demonstrating the potentiality and advisability of an alternative formulation as a new adjuvant option for future immunizations to produce C. d. terrificus antivenom.
description Fil: Fusco, Luciano S. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-25
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Fusco, Luciano S., et. al, 2022. Corrigendum to “CpG-ODN formulated with a nanostructure as adjuvant for anticrotalic serum production. Studies in mice” [Toxicon 215 (2022) 28–36]. Toxicon: Países BajosElsevier Ltd., p. 1-1, ISSN 0041-0101.
0041-0101
http://repositorio.unne.edu.ar/handle/123456789/58048
identifier_str_mv Fusco, Luciano S., et. al, 2022. Corrigendum to “CpG-ODN formulated with a nanostructure as adjuvant for anticrotalic serum production. Studies in mice” [Toxicon 215 (2022) 28–36]. Toxicon: Países BajosElsevier Ltd., p. 1-1, ISSN 0041-0101.
0041-0101
url http://repositorio.unne.edu.ar/handle/123456789/58048
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.toxicon.2022.07.002
https://doi.org/10.1016/j.toxicon.2022.05.045
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
p. 1-1
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ltd.
publisher.none.fl_str_mv Elsevier Ltd.
dc.source.none.fl_str_mv Toxicon, 2022, vol. 216, p. 114.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
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score 12.558318

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